Located in the sub-basement of Whistler,
in rooms S039 and S045, The Purdue Genomics Core Facility provides both low-throughput
and high-throughput DNA sequencing, Affymetrix GeneChip processing, colony
picking, library duplication, rearraying and filter printing services on a
recharge basis.
The following are the services currently
available as Purdue University. Most of these services are also available to
users outside Purdue. Charges will be approximately 1.52X for external users. Contact
us for exact prices.
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Bring us your DNA, a
primer and we return a sequence read to you. More details available here: LTL. Currently $9.20/sample or
$5.60/sample for Purdue Cancer Center Members. |
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We sequence from 96-well
or 384-well plates of glycerol stocks. Contact
us if you have a large job. These
prices include minipreps, sequencing reactions, clean-ups, runs on our ABI
3730XL sequencers and some data analysis (e.g. blast searches or sequence assembly.)
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$264.40 for four 22x22 cm
filters. Usually 18,432 glycerol stocks (in 384-well plates) are spotted per
filter. We incubate the filters, lyse the resulting bacterial colonies and
crosslink their DNA to the filter using UV irradiation. |
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Bring your titred
transformation mix. We plate the bacteria on appropriate media and pick the
resulting colonies into 384-well plate glycerol stocks. $10.60/plate of
glycerol stocks requested. |
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Replicate a library of
glycerol stocks in 96 or 384 well plates. If your library is in Genetix
384-well plates the charge is $5/plate duplicated. If your library is not in
Genetix 384-well plates we have to use a more cumbersome method of
duplication for which the charge is $6.70/plate. |
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Bring your library of
glycerol stocks in 384-well plates (source plates) and a list of the plate
and wells you wish to be placed in new destination plates. In most cases a
fresh replicate of the library must be used for good results. Charge is $7.60
for each source and destination plate. For example, rearraying the contents
of 9 source plates into 1 destination plate would cost $76. |
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Bring us DNA, we
mechanically shear it to a specified size-range, blunt the resulting
fragments and clone into the Invitrogen vector, pCR4TOPO, and electroporate
into DH10B host cells. Charge is $251.50—not including cost of colony
picking. |
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Bring us total RNA and
the Affy chip you want run. For single-cycle labeling protocol (works best
with >5000 ng of total RNA, 500 ng minimum) the charge is $249.10. For two-cycle
labeling (10 ng minimum total RNA) the charge is $313.10. More information
here: Affy |