(Linear) MAP of: ./Fgr-Venn_Contig0258.seq check: 882 from: 1 to: 2067 Contig258 Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * January 12, 2002 14:43 .. DrdI MluI| HindIII EarI || | | TCGACCCACGCGTCCGCAATCAACCACACTCGCTACAATGAAGCTTCAACTTTCTCTTCTCGGGCTGGTCGTCCTGGCCTTTGCAAAGCCACAAGACCCT 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 AGCTGGGTGCGCAGGCGTTAGTTGGTGTGAGCGATGTTACTTCGAAGTTGAAAGAGAAGAGCCCGACCAGCAGGACCGGAAACGTTTCGGTGTTCTGGGA a S T H A S A I N H T R Y N E A S T F S S R A G R P G L C K A T R P F - b R P T R P Q S T T L A T M K L Q L S L L G L V V L A F A K P Q D P - c D P R V R N Q P H S L Q * S F N F L F S G W S S W P L Q S H K T L - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d R G V R G C D V V S A V I F S * S E R R P S T T R A K A F G C S G - e V W A D A I L W V R * L S A E V K E E R A P R G P R Q L A V L G - f S G R T R L * G C E S C H L K L K R K E P Q D D Q G K C L W L V R - BseRI BsgI PvuII EarI BseRI BpmI |AlwNI | BpmI BpmI | AceIII BseRI| BsmBI | | | | | | | | || | TCTGATCCAGCCCCTGCTCCTCCAGTATCATCTGCACCAGCTGAACCTCCAGCTCCAGTTCCACTTCCCTCTTCTCAGCCGACTCCTCCTACGTCTCCGG 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 AGACTAGGTCGGGGACGAGGAGGTCATAGTAGACGTGGTCGACTTGGAGGTCGAGGTCAAGGTGAAGGGAGAAGAGTCGGCTGAGGAGGATGCAGAGGCC a * S S P C S S S I I C T S * T S S S S S T S L F S A D S S Y V S G - b S D P A P A P P V S S A P A E P P A P V P L P S S Q P T P P T S P V - c L I Q P L L L Q Y H L H Q L N L Q L Q F H F P L L S R L L L R L R - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d E S G A G A G G T D D A G A S G G A G T G S G E E * G V G G V D G - e K Q D L G Q E E L I M Q V L Q V E L E L E V E R K E A S E E * T E P - f R I W G R S R W Y * R C W S F R W S W N W K G R R L R S R R R R R - AceIII BsgI BspEI | SalI | DrdI KpnI BspMI RsrII | | SmaI | | MluI| | | | | | | | | || TACCTGCTCCTCCTGGCTGATCCAGCTCCGGTCCGGATTCCCGGGTCGACCCACGCGTCCGGCTGCACCTACCCCAGAAGGACCAATCTGCGAGTGTGGA 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 ATGGACGAGGAGGACCGACTAGGTCGAGGCCAGGCCTAAGGGCCCAGCTGGGTGCGCAGGCCGACGTGGATGGGGTCTTCCTGGTTAGACGCTCACACCT a T C S S W L I Q L R S G F P G R P T R P A A P T P E G P I C E C G - b P A P P G * S S S G P D S R V D P R V R L H L P Q K D Q S A S V D - c Y L L L L A D P A P V R I P G S T H A S G C T Y P R R T N L R V W I - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d T G A G G P Q D L E P G S E R T S G R T R S C R G W F S W D A L T S - e V Q E E Q S I W S R D P N G P R G V R G A A G V G S P G I Q S H P - f Y R S R R A S G A G T R I G P D V W A D P Q V * G L L V L R R T H - BbsI Eco57I NcoI BclI | | | | | TATACCTACTGTGCTGAAGTTCTCAAAGCGATGCCTGACAAGCCATGGAGTGATGATCAACTGTCTTCTGCGTACTGCAAGACCCCTAATGCAGTTTGCA 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 ATATGGATGACACGACTTCAAGAGTTTCGCTACGGACTGTTCGGTACCTCACTACTAGTTGACAGAAGACGCATGACGTTCTGGGGATTACGTCAAACGT a Y T Y C A E V L K A M P D K P W S D D Q L S S A Y C K T P N A V C N - b I P T V L K F S K R C L T S H G V M I N C L L R T A R P L M Q F A - c Y L L C * S S Q S D A * Q A M E * * S T V F C V L Q D P * C S L Q - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d I G V T S F N E F R H R V L W P T I I L Q R R R V A L G R I C N A - e Y V * Q A S T R L A I G S L G H L S S * S D E A Y Q L V G L A T Q - f I Y R S H Q L E * L S A Q C A M S H H D V T K Q T S C S G * H L K C - SalI BstXI | | ACGGGCAGTCACCAGCAAGCAAGGTCGACAACGCTCTGTTCATTTGTCTCTGTGCCCAACCTGACCAAAAGGTTGGAGATCACATTGAACTTCTGTGCGG 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 TGCCCGTCAGTGGTCGTTCGTTCCAGCTGTTGCGAGACAAGTAAACAGAGACACGGGTTGGACTGGTTTTCCAACCTCTAGTGTAACTTGAAGACACGCC a G Q S P A S K V D N A L F I C L C A Q P D Q K V G D H I E L L C G - b T G S H Q Q A R S T T L C S F V S V P N L T K R L E I T L N F C A A - c R A V T S K Q G R Q R S V H L S L C P T * P K G W R S H * T S V R - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d V P L * W C A L D V V S Q E N T E T G L R V L L N S I V N F K Q A - e L P C D G A L L T S L A R N M Q R Q A W G S W F T P S * M S S R H P - f R A T V L L C P R C R E T * K D R H G V Q G F P Q L D C Q V E T R - BspLU11I AceIII MscI | | | CTGCGATACATGTCTTAATGTTGGGCCAGACTTTAGAGGACGGTGCAAGAGTCCTTGCAGAGCTGGCCACGGAAAGGGAGGCAGTGGTGGTGGTAAAGGA 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 GACGCTATGTACAGAATTACAACCCGGTCTGAAATCTCCTGCCACGTTCTCAGGAACGTCTCGACCGGTGCCTTTCCCTCCGTCACCACCACCATTTCCT a C D T C L N V G P D F R G R C K S P C R A G H G K G G S G G G K G - b A I H V L M L G Q T L E D G A R V L A E L A T E R E A V V V V K E - c L R Y M S * C W A R L * R T V Q E S L Q S W P R K G R Q W W W * R R - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d A A I C T K I N P W V K S S P A L T R A S S A V S L S A T T T T F S - e Q S V H R L T P G S K L P R H L L G Q L A P W P F P P L P P P L P - f S R Y M D * H Q A L S * L V T C S D K C L Q G R F P L C H H H Y L - HindIII BseRI | | GGTGGCAAGATCAGGGGAAACAATCGGATGGGATTTTGGTAGAGATGAAGTGATGGATGTAATGGTGGATTGAGGAGGAAGCTTTACATGATTCAAATTG 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 CCACCGTTCTAGTCCCCTTTGTTAGCCTACCCTAAAACCATCTCTACTTCACTACCTACATTACCACCTAACTCCTCCTTCGAAATGTACTAAGTTTAAC a G G K I R G N N R M G F W * R * S D G C N G G L R R K L Y M I Q I D - b V A R S G E T I G W D F G R D E V M D V M V D * G G S F T * F K L - c W Q D Q G K Q S D G I L V E M K * W M * W W I E E E A L H D S N * - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d T A L D P S V I P H S K P L S S T I S T I T S Q P P L K V H N L N - e P P L I L P F L R I P N Q Y L H L S P H L P P N L L F S * M I * I - f L H C S * P F C D S P I K T S I F H H I Y H H I S S S A K C S E F Q - XbaI BsrBI | MluI EagI | | HindIII BsiWI | EarI NotI | | BamHI | SnaBI | SphI AatII SapI | | | | | | | | | | ACACATTCTTTACCAAAAAAAAAAAAAAAGGGCGGCCGCTCTAGAGGATCCAAGCTTACGTACGCGTGCATGCGACGTCATAGCTCTTCTATAGTGTCAC 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 TGTGTAAGAAATGGTTTTTTTTTTTTTTTCCCGCCGGCGAGATCTCCTAGGTTCGAATGCATGCGCACGTACGCTGCAGTATCGAGAAGATATCACAGTG a T F F T K K K K K G R P L * R I Q A Y V R V H A T S * L F Y S V T - b T H S L P K K K K K G G R S R G S K L T Y A C M R R H S S S I V S P - c H I L Y Q K K K K R A A A L E D P S L R T R A C D V I A L L * C H - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d V C E K G F F F F F P P R E L P D L S V Y A H M R R * L E E I T D - e S V N K V L F F F F P R G S * L I W A * T R T C A V D Y S K * L T V - f C M R * W F F F F L A A A R S S G L K R V R A H S T M A R R Y H * - CTAAATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 GATTTAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN a * I ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? - b K ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? - c L N ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? - 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 d G L N ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? - e * I ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? - f R F ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? - NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGACGCGCCCTGTAGCGGCGCATTAAGCGCG 901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1000 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGCGCGGGACATCGCCGCGTAATTCGCGC a ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? R A L * R R I K R G - b ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? D A P C S G A L S A - c ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? T R P V A A H * A R - 901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1000 d ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? S A G Q L P A N L A - e ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? R A R Y R R M L R - f ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? V R G T A A C * A R - BsrBI NgoAIV | | GCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCG 1001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1100 CGCCCACACCACCAATGCGCGTCGCACTGGCGATGTGAACGGTCGCGGGATCGCGGGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGGC a G C G G Y A Q R D R Y T C Q R P S A R S F R F L P F L S R H V R R - b A G V V V T R S V T A T L A S A L A P A P F A F F P S F L A T F A G - c R V W W L R A A * P L H L P A P * R P L L S L S S L P F S P R S P - 1001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1100 d A P T T T V R L T V A V S A L A R A G A G K A K K G E K R A V N A - e P P H P P * A C R S R * V Q W R G L A R E K R K R G K R E R W T R R - f R T H H N R A A H G S C K G A G * R G S R E S E E R G K E G R E G - DrdII | GCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTC 1101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1200 CGAAAGGGGCAGTTCGAGATTTAGCCCCCGAGGGAAATCCCAAGGCTAAATCACGAAATGCCGTGGAGCTGGGGTTTTTTGAACTAATCCCACTACCAAG a L S P S S S K S G A P F R V P I * C F T A P R P Q K T * L G * W F - b F P R Q A L N R G L P L G F R F S A L R H L D P K K L D * G D G S - c A F P V K L * I G G S L * G S D L V L Y G T S T P K N L I R V M V H - 1101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1200 d P K G R * A R F R P S G K P N R N L A K R C R S G L F S S * P S P E - e S E G D L E L D P A G K L T G I * H K V A G R G W F V Q N P H H N - f A K G T L S * I P P E R * P E S K T S * P V E V G F F K I L T I T - BsaXI DraIII DrdI | BsbI | | | | ACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCA 1201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1300 TGCATCACCCGGTAGCGGGACTATCTGCCAAAAAGCGGGAAACTGCAACCTCAGGTGCAAGAATTATCACCTGAGAACAAGGTTTGACCTTGTTGTGAGT a T * W A I A L I D G F S P F D V G V H V L N S G L L F Q T G T T L N - b R S G P S P * * T V F R P L T L E S T F L I V D S C S K L E Q H S - c V V G H R P D R R F F A L * R W S P R S * * W T L V P N W N N T Q - 1201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1300 d R L P G D G Q Y V T K R G K V N S D V N K I T S E Q E L S S C C E - e V Y H A M A R I S P K E G K S T P T W T R L L P S K N W V P V V S - f * T T P W R G S L R N K A R Q R Q L G R E * Y H V R T G F Q F L V * - AceIII | ACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTT 1301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1400 TGGGATAGAGCCAGATAAGAAAACTAAATATTCCCTAAAACGGCTAAAGCCGGATAACCAATTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTTAAA a P I S V Y S F D L * G I L P I S A Y W L K N E L I * Q K F N A N F - b T L S R S I L L I Y K G F C R F R P I G * K M S * F N K N L T R I L - c P Y L G L F F * F I R D F A D F G L L V K K * A D L T K I * R E F - 1301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1400 d V R D R D I R K I * L P N Q R N R G I P * F I L Q N L L F K V R I - e L G I E T * E K S K Y P I K G I E A * Q N F F S S I * C F N L A F K - f G * R P R N K Q N I L S K A S K P R N T L F H A S K V F I * R S N - SspI | TAACAAAATATTAACGCTTACAATTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1500 ATTGTTTTATAATTGCGAATGTTAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN a N K I L T L T I ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? - b T K Y * R L Q F ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? - c * Q N I N A Y N ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? - 1401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1500 d K V F Y * R K C N ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? - e L L I N V S V I ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? - f * C F I L A * L K ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? - NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1600 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN a ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? - b ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? - c ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? - 1501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1600 d ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? - e ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? - f ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? - ApaI XhoI | | NNNNNNNNNNNNNNNNNNNTACCGGGCCCCCCCTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGAGAGTTGTTAATATGTATTCAAAGAACTCCTA 1601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1700 NNNNNNNNNNNNNNNNNNNATGGCCCGGGGGGGAGCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCTCTCAACAATTATACATAAGTTTCTTGAGGAT a ? ? ? ? ? ? T G P P L E F F F F F F F F F S R V V N M Y S K N S Y - b ? ? ? ? ? ? ? P G P P S S F F F F F F F F F R E L L I C I Q R T P M - c ? ? ? ? ? ? Y R A P P R V F F F F F F F F F E S C * Y V F K E L L - 1601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1700 d ? ? ? ? ? ? ? G P G G E L K K K K K K K K K R S N N I H I * L V G - e ? ? ? ? ? ? ? V P G G R S N K K K K K K K K E L T T L I Y E F F E * - f ? ? ? ? ? ? ? R A G G R T K K K K K K K K K S L Q * Y T N L S S R - EarI PinAI BfiI SapI BbsI | BpmI | | | | | TGGCTATCTCATCGCTTTGAGAGCTTACTGGGGGAACTTGAAGCCGGGAGGGACACAGTAGGGGCTGGCGCTCTTCTTGTAGAAGACACAACCGGTCTTG 1701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1800 ACCGATAGAGTAGCGAAACTCTCGAATGACCCCCTTGAACTTCGGCCCTCCCTGTGTCATCCCCGACCGCGAGAAGAACATCTTCTGTGTTGGCCAGAAC a G Y L I A L R A Y W G N L K P G G T Q * G L A L F L * K T Q P V L - b A I S S L * E L T G G T * S R E G H S R G W R S S C R R H N R S W - c W L S H R F E S L L G E L E A G R D T V G A G A L L V E D T T G L G - 1701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1800 d I A I E D S Q S S V P P V Q L R S P C L L P Q R E E Q L L C L R D Q - e P * R M A K L A * Q P F K F G P P V C Y P S A S K K Y F V C G T K - f H S D * R K S L K S P S S S A P L S V T P A P A R R T S S V V P R - AlwNI PflMI BspMI KpnI BsgI BsmBI| | | | | || GGAGGGCAAACTCCAGCAGCAACCCACTGGCTGGTGACATCGCAGACCTGCCAGCCACTCTTGTCGGGAGTGCAGGAGTAGGTACCAGGCTTGCAGCCGC 1801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1900 CCTCCCGTTTGAGGTCGTCGTTGGGTGACCGACCACTGTAGCGTCTGGACGGTCGGTGAGAACAGCCCTCACGTCCTCATCCATGGTCCGAACGTCGGCG a G G Q T P A A T H W L V T S Q T C Q P L L S G V Q E * V P G L Q P L - b E G K L Q Q Q P T G W * H R R P A S H S C R E C R S R Y Q A C S R - c R A N S S S N P L A G D I A D L P A T L V G S A G V G T R L A A A - 1801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1900 d S P L S W C C G V P Q H C R L G A L W E Q R S H L L L Y W A Q L R - e P P C V G A A V W Q S T V D C V Q W G S K D P T C S Y T G P K C G - f P L A F E L L L G S A P S M A S R G A V R T P L A P T P V L S A A A - BsaXI BssSI | | TGGGAGACGCGGCGGCGACGGAGGCGAGAGCAGTGATGGCGACGGCGGTGAACTTCATGTTTGCGGTTGGTGGTTTGTTTGGAGANAGTGTGCCTCGTGC 1901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 2000 ACCCTCTGCGCCGCCGCTGCCTCCGCTCTCGTCACTACCGCTGCCGCCACTTGAAGTACAAACGCCAACCACCAAACAAACCTCTNTCACACGGAGCACG a G D A A A T E A R A V M A T A V N F M F A V G G L F G ? S V P R A - b W E T R R R R R R E Q * W R R R * T S C L R L V V C L E ? V C L V P - c G R R G G D G G E S S D G D G G E L H V C G W W F V W R ? C A S C - 1901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 2000 d Q S V R R R R L R S C H H R R R H V E H K R N T T Q K S ? T H R T - e S P S A A A V S A L A T I A V A T F K M N A T P P K N P S L T G R A - f P L R P P S P P S L L S P S P P S S * T Q P Q H N T Q L ? H A E H - BamHI EagI SmaI | NotI EcoRI PstI | | SpeI XbaI BsrBI|BpmI SacII SacI | | | | | | || | | | CGAATTCCTGCAGCCCGGGGGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGGGAGCTCCAGCTTT 2001 ---------+---------+---------+---------+---------+---------+------- 2067 GCTTAAGGACGTCGGGCCCCCTAGGTGATCAAGATCTCGCCGGCGGTGGCGCCCCTCGAGGTCGAAA a E F L Q P G G S T S S R A A A T A G S S S F - b N S C S P G D P L V L E R P P P R G A P A - c R I P A A R G I H * F * S G R H R G E L Q L - 2001 ---------+---------+---------+---------+---------+---------+------- 2067 d G F E Q L G P S G S T R S R G G G R P A G A K - e S N R C G P P D V L E L A A A V A P L E L K - f R I G A A R P I W * N * L P R W R P S S W S - Enzymes that do cut: AatII AceIII AlwNI ApaI BamHI BbsI BclI BfiI BpmI BsaXI BsbI BseRI BsgI BsiWI BsmBI BspEI BspLU11I BspMI BsrBI BssSI BstXI DraIII DrdI DrdII EagI EarI Eco57I EcoRI HindIII KpnI MluI MscI NcoI NgoAIV NotI PflMI PinAI PstI PvuII RsrII SacI SacII SalI SapI SmaI SnaBI SpeI SphI SspI XbaI XhoI Enzymes that do not cut: AflII AhdI ApaBI ApaLI AscI AvrII Bce83I BcgI BglI BglII BplI Bpu10I Bpu1102I BsaI BsaBI BsmI BspGI BsrDI BsrGI BssHII Bst1107I BstEII Bsu36I ClaI DraI EciI Eco47III EcoNI EcoRV FseI FspI HgiEII HpaI MfeI NarI NdeI NheI NruI NsiI NspV PacI Pfl1108I PmeI PmlI PshAI Psp1406I PvuI RcaI SanDI ScaI SexAI SfiI SgfI SgrAI SrfI Sse8387I Sse8647I StuI SwaI Tth111I VspI XcmI XmnI