The query sequence for this search has been filtered. Filtering
eliminates low complexity regions that commonly give spuriously high
scores that reflect compositional bias rather than significant
position-by-position alignment. Filtering can eliminate these potentially
confounding matches (e.g., hits against proline-rich regions or poly-A
tails) from the blast reports, leaving regions whose blast statistics
reflect the specificity of their pairwise alignment.
BLASTX 2.0.13 [May-26-2000]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fgr_0_e05_t3_018.ab1.seq Fgr_0_E05_T3_018.ab1 0 0 0 1
837
(830 letters)
Database: nr
509,459 sequences; 159,777,284 total letters
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_033456.1| trypsin 2 >gi|136428|sp|P07146|TRYP_MOUSE ... 35 1.1
ref|NP_035776.1| trypsin 4 >gi|2358085|gb|AAB69056.1| (AE00... 33 4.2
gb|AAB69057.1| (AE000664) trypsinogen 9 [Mus musculus] >gi|... 33 4.2
sp|P17923|NFIA_CHICK NUCLEAR FACTOR 1 A1 (NF-I) (CCAAT BOX-... 32 7.2
gb|AAF56722.1| (AE003761) CG5639 gene product [Drosophila m... 32 9.4
>ref|NP_033456.1| trypsin 2
sp|P07146|TRYP_MOUSE TRYPSINOGEN PRECURSOR
pir||B25528 trypsin (EC 3.4.21.4) precursor - mouse
emb|CAA28243.1| (X04574) preprotrypsin (246 aa) [Mus musculus]
gb|AAB69089.1| (AE000665) trypsinogen 20 [Mus musculus]
dbj|BAA74759.1| (AB017030) pancreatic trypsin [Mus musculus]
Length = 246
Score = 34.8 bits (78), Expect = 1.1
Identities = 16/51 (31%), Positives = 26/51 (50%)
Frame = +2
Query: 17 HKNHSTRYAPTTTLIMKLIPLVTMAAFVAATPAPKGCTPGTYSCMADLKGW 169
H N+++ +++KL VT+ A VA+ P P C P C+ + GW
Sbjct: 96 HPNYNSWTLDNDIMLIKLASPVTLNARVASVPLPSSCAPAGTQCL--ISGW 144
>ref|NP_035776.1| trypsin 4
gb|AAB69056.1| (AE000664) trypsinogen 8 [Mus musculus]
dbj|BAA74761.1| (AB017032) pancreatic trypsin [Mus musculus]
Length = 246
Score = 32.8 bits (73), Expect = 4.2
Identities = 16/51 (31%), Positives = 24/51 (46%)
Frame = +2
Query: 17 HKNHSTRYAPTTTLIMKLIPLVTMAAFVAATPAPKGCTPGTYSCMADLKGW 169
H N ++R +++KL VT+ A VA P C P C+ + GW
Sbjct: 96 HPNFNSRTLNNDIMLIKLASPVTLNARVATVALPSSCAPAGTQCL--ISGW 144
>gb|AAB69057.1| (AE000664) trypsinogen 9 [Mus musculus]
dbj|BAA74760.1| (AB017031) TESP4 [Mus musculus]
dbj|BAA85187.1| (AB009661) TESP4 [Mus musculus]
Length = 246
Score = 32.8 bits (73), Expect = 4.2
Identities = 16/51 (31%), Positives = 24/51 (46%)
Frame = +2
Query: 17 HKNHSTRYAPTTTLIMKLIPLVTMAAFVAATPAPKGCTPGTYSCMADLKGW 169
H N ++R +++KL VT+ A VA P C P C+ + GW
Sbjct: 96 HPNFNSRTLNNDIMLIKLASPVTLNARVATVALPSSCAPAGTQCL--ISGW 144
>sp|P17923|NFIA_CHICK NUCLEAR FACTOR 1 A1 (NF-I) (CCAAT BOX-BINDING TRANSCRIPTION FACTOR)
(CTF) (TGGCA-BINDING PROTEIN)
pir||S09996 nuclear factor I-A1 - chicken
emb|CAA35853.1| (X51486) cNFI-A1 protein (AA 1-522) [Gallus gallus]
Length = 522
Score = 32.1 bits (71), Expect = 7.2
Identities = 24/66 (36%), Positives = 36/66 (54%), Gaps = 13/66 (19%)
Frame = +3
Query: 192 PGW*ASPSFSGALTRERKLTGMRLSFK--FAGACPPKTACL---FNKHN--------GSP 332
PG+ +P GAL T ++ S K F+ P +T+ L F +H+ SP
Sbjct: 315 PGYLRNPEHRGALHGMPSPTALKKSEKSGFSSPSPSQTSSLGTAFTQHHRPVITGPRASP 374
Query: 333 YCVPPGLHF*ASSVLD*PG 389
+ P LHF S ++ PG
Sbjct: 375 HATPSTLHFPTSPIIQQPG 393
>gb|AAF56722.1| (AE003761) CG5639 gene product [Drosophila melanogaster]
Length = 1511
Score = 31.7 bits (70), Expect = 9.4
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 285 CPPKTACLFNKHNGSPYCVPPG 350
CPP ACL K PY VPPG
Sbjct: 772 CPPVPACLPRKPGQCPYLVPPG 793
Database: nr
Posted date: Jun 11, 2000 9:46 PM
Number of letters in database: 159,777,284
Number of sequences in database: 509,459
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.270 0.0470 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 201388120
Number of Sequences: 509459
Number of extensions: 4164467
Number of successful extensions: 12970
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 12965
Number of HSP's gapped (non-prelim): 8
length of query: 276
length of database: 159,777,284
effective HSP length: 55
effective length of query: 221
effective length of database: 131,757,039
effective search space: 29118305619
effective search space used: 29118305619
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 70 (31.7 bits)