The query sequence for this search has been filtered. Filtering
eliminates low complexity regions that commonly give spuriously high
scores that reflect compositional bias rather than significant
position-by-position alignment. Filtering can eliminate these potentially
confounding matches (e.g., hits against proline-rich regions or poly-A
tails) from the blast reports, leaving regions whose blast statistics
reflect the specificity of their pairwise alignment.
BLASTX 2.1.1 [Aug-8-2000]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Contig10.seq Contig10
(822 letters)
Database: nr
565,281 sequences; 177,575,912 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AAA85880.1| (U33331) orf UL141 [human herpesvirus 5] 32 6.1
pir||T16582 hypothetical protein K08A8.3 - Caenorhabditis e... 32 6.1
>gb|AAA85880.1| (U33331) orf UL141 [human herpesvirus 5]
Length = 425
Score = 32.5 bits (72), Expect = 6.1
Identities = 30/86 (34%), Positives = 50/86 (57%), Gaps = 32/86 (37%)
Frame = -3
Query: 433 VGKTGDGDGN---DTARKTSRTMLGM---RVIQTL-STRPKQVMSVA------------- 314
VG+ GDG+G T +KT R++ G+ ++ +T+ +T P + +S
Sbjct: 21 VGRDGDGEGEAAKKTCKKTGRSVAGIPGEKLRRTVVTTTPARRLSGRHTEQEQAGMRLCE 80
Query: 313 ------VQCLLSVLSRMPWLYPVPIDFPRLISNTEDS------DINRPQWSSYREKCS 176
+ C L +PWL+ V + PRL+ + S DI W+ E S
Sbjct: 81 KGKKRIIMCRRESLRTLPWLFWVLLSCPRLLEYSSSSFPFATADIAEKMWAENYETTS 138
>pir||T16582 hypothetical protein K08A8.3 - Caenorhabditis elegans
gb|AAA79748.1| (U38377) weak similarity to M. musculus ubiquitin carboxyl-terminal
hydrolase (SP:UBP_MOUSE, P35123); similar to S. pombe
double-strand-break repair protein, RAD21
(SP:RA21_SCHPO,P30776) [Caenorhabditis elegans]
Length = 652
Score = 32.5 bits (72), Expect = 6.1
Identities = 13/29 (44%), Positives = 20/29 (68%)
Frame = +3
Query: 378 VRDVFRAVSLPSPSPVFPTHSSQDLFSSF 464
++ VFR+ +L P+PV PT S QD++ F
Sbjct: 140 IKLVFRSGALDQPNPVLPTFSIQDIYGDF 168
Database: nr
Posted date: Sep 29, 2000 9:53 PM
Number of letters in database: 177,575,912
Number of sequences in database: 565,281
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.270 0.0470 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 266245222
Number of Sequences: 565281
Number of extensions: 5548710
Number of successful extensions: 18841
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 18839
Number of HSP's gapped (non-prelim): 3
length of query: 274
length of database: 177,575,912
effective HSP length: 54
effective length of query: 219
effective length of database: 147,050,738
effective search space: 32204111622
effective search space used: 32204111622
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 70 (31.7 bits)