The query sequence for this search has been filtered. Filtering
eliminates low complexity regions that commonly give spuriously high
scores that reflect compositional bias rather than significant
position-by-position alignment. Filtering can eliminate these potentially
confounding matches (e.g., hits against proline-rich regions or poly-A
tails) from the blast reports, leaving regions whose blast statistics
reflect the specificity of their pairwise alignment.
TBLASTX 2.1.1 [Aug-8-2000]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Contig471.seq Contig471
(356 letters)
Database: preblast_est
5,879,525 sequences; -1,931,041,176 total letters
Score E
Sequences producing significant alignments: (bits) Value
dbj|AV540841.1|AV540841 AV540841 Arabidopsis thaliana roots... 32 7.9
dbj|AV613466.1|AV613466 AV613466 Bos taurus adipocyte cell ... 33 8.8
gb|AW345852.1|AW345852 15820 MARC 1BOV Bos taurus cDNA 5'. 33 8.8
>dbj|AV540841.1|AV540841 AV540841 Arabidopsis thaliana roots Columbia Arabidopsis thaliana
cDNA clone RZ156b01F 3'.
Length = 247
Score = 31.8 bits (63), Expect(2) = 7.9
Identities = 10/20 (50%), Positives = 17/20 (85%)
Frame = -3 / +1
Query: 339 TTITPFLNNFLPTTKQSSQW 280
+T+ +LN +LPTTK++S+W
Sbjct: 109 STVQSWLNRWLPTTKKTSRW 168
Score = 16.2 bits (29), Expect(2) = 7.9
Identities = 8/17 (47%), Positives = 10/17 (58%)
Frame = -2 / +2
Query: 160 PSLGQDTSFDDESRTEL 110
P G +SF + SRT L
Sbjct: 158 PPGGAPSSFINLSRTTL 208
>dbj|AV613466.1|AV613466 AV613466 Bos taurus adipocyte cell line Bos taurus cDNA clone
E0AD005F07 5'.
Length = 422
Score = 32.7 bits (65), Expect = 8.8
Identities = 10/13 (76%), Positives = 11/13 (83%)
Frame = -3 / -3
Query: 153 WVKIHPLTTNHGR 115
W+KIHP TT HGR
Sbjct: 141 WIKIHPFTTPHGR 103
>gb|AW345852.1|AW345852 15820 MARC 1BOV Bos taurus cDNA 5'.
Length = 419
Score = 32.7 bits (65), Expect = 8.8
Identities = 10/13 (76%), Positives = 11/13 (83%)
Frame = -3 / -2
Query: 153 WVKIHPLTTNHGR 115
W+KIHP TT HGR
Sbjct: 151 WIKIHPFTTPHGR 113
Database: preblast_est
Posted date: Sep 30, 2000 7:07 PM
Number of letters in database: -1,931,041,176
Number of sequences in database: 5,879,525
Lambda K H
0.318 0.135 0.00
Matrix: BLOSUM62
Number of Hits to DB: 1st pass: 1516306599, 2nd pass: 158223
Number of Sequences: 1st pass: 5879525, 2nd pass: 2850
Number of extensions: 1st pass: 11995888, 2nd pass: 131158
Number of successful extensions: 1st pass: 475, 2nd pass: 1007
Number of sequences better than 10.0: 4
length of query: 118
length of database: 787,975,373
effective HSP length: 54
effective length of query: 64
effective length of database: 470,481,023
effective search space: 30110785472
effective search space used: 30110785472
frameshift window, decay const: 50, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)
S2: 63 (31.8 bits)