(Linear) MAP of: ./Contig018.seq check: 3568 from: 1 to: 802 Fgr.fasta.screen.Contig18 Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * March 20, 2001 16:46 .. EarI EcoRI BssSI BspMI XmnI EarI NruI | | | | | | GAATTCGGCACGAGGACTCAACCTGCGCTGGAAAACGCAGAGAAACTCTTCATAACACCAAAACCCCCATCCGATAAAAACTCTTCGCGAACGAGCTTCG 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 CTTAAGCCGTGCTCCTGAGTTGGACGCGACCTTTTGCGTCTCTTTGAGAAGTATTGTGGTTTTGGGGGTAGGCTATTTTTGAGAAGCGCTTGCTCGAAGC a E F G T R T Q P A L E N A E K L F I T P K P P S D K N S S R T S F V - b N S A R G L N L R W K T Q R N S S * H Q N P H P I K T L R E R A S - c I R H E D S T C A G K R R E T L H N T K T P I R * K L F A N E L R - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d I R C S S E V Q A P F R L S V R * L V L V G M R Y F S K A F S S R - e F E A R P S L R R Q F V C L F E E Y C W F G W G I F V R R S R A E - f N P V L V * G A S S F A S F S K M V G F G G D S L F E E R V L K - BsrDI KpnI NheI | | | TAACGCGCTGTCGCAATGGGCGCTCTTTTGTCTCTACCGCTTCTTGCGGTACCCAGCGTCGGTACTATTGTATCCTTCGCTGCTAGCTGCTGCGGTGCTG 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 ATTGCGCGACAGCGTTACCCGCGAGAAAACAGAGATGGCGAAGAACGCCATGGGTCGCAGCCATGATAACATAGGAAGCGACGATCGACGACGCCACGAC a T R C R N G R S F V S T A S C G T Q R R Y Y C I L R C * L L R C C - b * R A V A M G A L L S L P L L A V P S V G T I V S F A A S C C G A A - c N A L S Q W A L F C L Y R F L R Y P A S V L L Y P S L L A A A V L - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d L A S D C H A S K Q R * R K K R Y G A D T S N Y G E S S A A A T S - e Y R A T A I P A R K D R G S R A T G L T P V I T D K A A L Q Q P A - f T V R Q R L P R E K T E V A E Q P V W R R Y * Q I R R Q * S S R H Q - BseRI AlwNI NdeI | EcoRI NcoI PstI |BcgI BcgI | | BssSI | | | | | | | | | | CGACCTGTTCCATGGTCTGCAGTGCCTGTGGCAAGTGCGGCAATAGTGTCGCAACCCGTATCGCATATGCGCTCCTTCTCCTCGTGAATTCGATTATAGC 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 GCTGGACAAGGTACCAGACGTCACGGACACCGTTCACGCCGTTATCACAGCGTTGGGCATAGCGTATACGCGAGGAAGAGGAGCACTTAAGCTAATATCG a D L F H G L Q C L W Q V R Q * C R N P Y R I C A P S P R E F D Y S - b T C S M V C S A C G K C G N S V A T R I A Y A L L L L V N S I I A - c R P V P W S A V P V A S A A I V S Q P V S H M R S F S S * I R L * L - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d R G T G H D A T G T A L A A I T D C G T D C I R E K E E H I R N Y - e A V Q E M T Q L A Q P L H P L L T A V R I A Y A S R R R T F E I I A - f S R N W P R C H R H C T R C Y H R L G Y R M H A G E G R S N S * L - Eco57I Tth111I NcoI | HindIII MfeI BspGI | DrdII | | | | | | | TTGGATAATGCTCACGCCATGGGCAATCAAAAAGCTTCAGAAACTCACACTCGATTACGTTACGATCAATTGTCCAACTGGACAATGTCACGGCTGGTTC 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 AACCTATTACGAGTGCGGTACCCGTTAGTTTTTCGAAGTCTTTGAGTGTGAGCTAATGCAATGCTAGTTAACAGGTTGACCTGTTACAGTGCCGACCAAG a L D N A H A M G N Q K A S E T H T R L R Y D Q L S N W T M S R L V R - b W I M L T P W A I K K L Q K L T L D Y V T I N C P T G Q C H G W F - c G * C S R H G Q S K S F R N S H S I T L R S I V Q L D N V T A G S - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d S P Y H E R W P C D F L K L F E C E I V N R D I T W S S L T V A P E - e Q I I S V G H A I L F S * F S V S S * T V I L Q G V P C H * P Q N - f K S L A * A M P L * F A E S V * V R N R * S * N D L Q V I D R S T - EarI BsaI | BssSI | | | GCAGTCCATCGAATCAACTTCGCCCTCGGTCTCTTCCATCTCGTCCTAGCTGGTTTGCTTTTCGGTGTCGCAACCTCCAAGAATCCTCGTGCCTCTATTC 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 CGTCAGGTAGCTTAGTTGAAGCGGGAGCCAGAGAAGGTAGAGCAGGATCGACCAAACGAAAAGCCACAGCGTTGGAGGTTCTTAGGAGCACGGAGATAAG a S P S N Q L R P R S L P S R P S W F A F R C R N L Q E S S C L Y S - b A V H R I N F A L G L F H L V L A G L L F G V A T S K N P R A S I Q - c Q S I E S T S P S V S S I S S * L V C F S V S Q P P R I L V P L F - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d C D M S D V E G E T E E M E D * S T Q K E T D C G G L I R T G R N - e A T W R I L K A R P R K W R T R A P K S K P T A V E L F G R A E I - f R L G D F * S R G R D R G D R G L Q N A K R H R L R W S D E H R * E - SanDI BfiI | BcgI | | | AGAATGGCTACTGGGGTCCCAAGATTATCGCCTGGCTCGCCTTTGTTGTTCTTTCTTTCCTGATTCCCGACGAGTTCTTTTTATTCTGGGGCAACTATAT 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 TCTTACCGATGACCCCAGGGTTCTAATAGCGGACCGAGCGGAAACAACAAGAAAGAAAGGACTAAGGGCTGCTCAAGAAAAATAAGACCCCGTTGATATA a E W L L G S Q D Y R L A R L C C S F F P D S R R V L F I L G Q L Y - b N G Y W G P K I I A W L A F V V L S F L I P D E F F L F W G N Y I - c R M A T G V P R L S P G S P L L F F L S * F P T S S F Y S G A T I S - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d L I A V P T G L N D G P E G K N N K R E Q N G V L E K * E P A V I - e * F P * Q P G L I I A Q S A K T T R E K R I G S S N K K N Q P L * I - f S H S S P D W S * R R A R R Q Q E K K G S E R R T R K I R P C S Y - BcgI PstI| BsmI ApaBI || EarI BfiI | | || | | CGCATTCGCTGCAGCTATGCTGTTCCTCTTCCTCGGCCTTATACTGCTTGTCGATCTTGCTCATAACTGGGCCGAATACTGCCTCGCCCAGATCGAGGAT 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 GCGTAAGCGACGTCGATACGACAAGGAGAAGGAGCCGGAATATGACGAACAGCTAGAACGAGTATTGACCCGGCTTATGACGGAGCGGGTCTAGCTCCTA a R I R C S Y A V P L P R P Y T A C R S C S * L G R I L P R P D R G Y - b A F A A A M L F L F L G L I L L V D L A H N W A E Y C L A Q I E D - c H S L Q L C C S S S S A L Y C L S I L L I T G P N T A S P R S R I - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d D C E S C S H Q E E E E A K Y Q K D I K S M V P G F V A E G L D L I - e A N A A A I S N R K R P R I S S T S R A * L Q A S Y Q R A W I S S - f R M R Q L * A T G R G R G * V A Q R D Q E Y S P R I S G R G S R P - XhoI BcgI BsrGI BsaI BcgI BsgI | | | | | | ACCGACTCGAGAAGTTGGCGATTCATCTTGATTGGCTCCACTCTGGGCATGTACATCGGGTCTCTTGCCATGACCATTGTGCAGTATATCTTTTTTGCGC 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 TGGCTGAGCTCTTCAACCGCTAAGTAGAACTAACCGAGGTGAGACCCGTACATGTAGCCCAGAGAACGGTACTGGTAACACGTCATATAGAAAAAACGCG a R L E K L A I H L D W L H S G H V H R V S C H D H C A V Y L F C A - b T D S R S W R F I L I G S T L G M Y I G S L A M T I V Q Y I F F A R - c P T R E V G D S S * L A P L W A C T S G L L P * P L C S I S F L R - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d G V R S T P S E D Q N A G S Q A H V D P R K G H G N H L I D K K R - e V S E L L Q R N M K I P E V R P M Y M P D R A M V M T C Y I K K A - f Y R S S F N A I * R S Q S W E P C T C R T E Q W S W Q A T Y R K Q A - GG 801 -- 802 CC a - b - c - 801 -- 802 d P - e R - f - Enzymes that do cut: AlwNI ApaBI BcgI BfiI BsaI BseRI BsgI BsmI BspGI BspMI BsrDI BsrGI BssSI DrdII EarI Eco57I EcoRI HindIII KpnI MfeI NcoI NdeI NheI NruI PstI SanDI Tth111I XhoI XmnI Enzymes that do not cut: AatII AceIII AflII AhdI ApaI ApaLI AscI AvrII BamHI BbsI Bce83I BclI BglI BglII BplI BpmI Bpu10I Bpu1102I BsaBI BsaXI BsbI BsiWI BsmBI BspEI BspLU11I BsrBI BssHII Bst1107I BstEII BstXI Bsu36I ClaI DraI DraIII DrdI EagI EciI Eco47III EcoNI EcoRV FseI FspI HgiEII HpaI MluI MscI NarI NgoAIV NotI NsiI NspV PacI Pfl1108I PflMI PinAI PmeI PmlI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI Sse8387I Sse8647I SspI StuI SwaI VspI XbaI XcmI