(Linear) MAP of: ./Contig021.seq check: 5602 from: 1 to: 1208 Fgr.fasta.screen.Contig21 Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * March 20, 2001 16:46 .. BamHI BssSI EcoRI BssSI | Bpu10I SacI | BcgI | | | | | | | GAATTCGGCACGAGGATCCTCCGAAACGACCCTGAGCTCAACCACGAGTACGCCCCCATCGCGGGTATTGCCAGCTTCACATCCAAGGCTGCCGAGCTAG 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 CTTAAGCCGTGCTCCTAGGAGGCTTTGCTGGGACTCGAGTTGGTGCTCATGCGGGGGTAGCGCCCATAACGGTCGAAGTGTAGGTTCCGACGGCTCGATC a E F G T R I L R N D P E L N H E Y A P I A G I A S F T S K A A E L V - b N S A R G S S E T T L S S T T S T P P S R V L P A S H P R L P S * - c I R H E D P P K R P * A Q P R V R P H R G Y C Q L H I Q G C R A S - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d N P V L I R R F S G S S L W S Y A G M A P I A L K V D L A A S S - e I R C S S G G F R G Q A * G R T R G W R P Y Q W S * M W P Q R A L - f F E A R P D E S V V R L E V V L V G G D R T N G A E C G L S G L * - Eco47III KpnI BcgI | BsbI PinAI| ApaLI SacI | | | || | | TCTTTGGCCCCGATTCTTCTGCCATCCAAGACAAGCGCTCAACAACACTACAGACAATCTCTGGTACCGGTGCCGTGCACTTGGGAGCTCTTTTCCTTGC 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 AGAAACCGGGGCTAAGAAGACGGTAGGTTCTGTTCGCGAGTTGTTGTGATGTCTGTTAGAGACCATGGCCACGGCACGTGAACCCTCGAGAAAAGGAACG a F G P D S S A I Q D K R S T T L Q T I S G T G A V H L G A L F L A - b S L A P I L L P S K T S A Q Q H Y R Q S L V P V P C T W E L F S L P - c L W P R F F C H P R Q A L N N T T D N L W Y R C R A L G S S F P C - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d T K P G S E E A M W S L R E V V S C V I E P V P A T C K P A R K R A - e R Q G R N K Q W G L C A S L L V V S L R Q Y R H R A S P L E K G Q - f D K A G I R R G D L V L A * C C * L C D R T G T G H V Q S S K E K - DrdII BglII BcgI PvuI | | | | CAAGTTCTACAAGGGCAACCAGACTGTCTACCTCTCAAATCCCACATGGGCGAACCACCACCAGATCTTCAAGAATGTTGGCATGTCGATCGACACATAC 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 GTTCAAGATGTTCCCGTTGGTCTGACAGATGGAGAGTTTAGGGTGTACCCGCTTGGTGGTGGTCTAGAAGTTCTTACAACCGTACAGCTAGCTGTGTATG a K F Y K G N Q T V Y L S N P T W A N H H Q I F K N V G M S I D T Y - b S S T R A T R L S T S Q I P H G R T T T R S S R M L A C R S T H T - c Q V L Q G Q P D C L P L K S H M G E P P P D L Q E C W H V D R H I P - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d L N * L P L W V T * R E F G V H A F W W W I K L F T P M D I S V Y - e W T R C P C G S Q R G R L D W M P S G G G S R * S H Q C T S R C M - f G L E V L A V L S D V E * I G C P R V V V L D E L I N A H R D V C V - FspI BcgI AhdI BsaI BbsI BsrBI SphI| | | | | | || CCCTACTTCCACAAGGAGACTAAGGGTCTCGATTTTGAGGGGTTCAAGAAGACTCTGCAATCCGCTCCCGAGGGTTCCGTCTTTGTGCTTCACGCATGCG 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 GGGATGAAGGTGTTCCTCTGATTCCCAGAGCTAAAACTCCCCAAGTTCTTCTGAGACGTTAGGCGAGGGCTCCCAAGGCAGAAACACGAAGTGCGTACGC a P Y F H K E T K G L D F E G F K K T L Q S A P E G S V F V L H A C A - b P T S T R R L R V S I L R G S R R L C N P L P R V P S L C F T H A - c L L P Q G D * G S R F * G V Q E D S A I R S R G F R L C A S R M R - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d G * K W L S V L P R S K S P N L F V R C D A G S P E T K T S * A H - e G R S G C P S * P D R N Q P T * S S E A I R E R P N R R Q A E R M R - f G V E V L L S L T E I K L P E L L S Q L G S G L T G D K H K V C A - XmnI PinAI SalI RcaI DrdII | | | | | | CACACAACCCTACCGGTGTCGACCCTACGCAAGATCAGTGGACTGAGATTGCTTCAATCATGAAGGAGAAGAACCACTTCCCCTTCTTTGACACCGCCTA 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 GTGTGTTGGGATGGCCACAGCTGGGATGCGTTCTAGTCACCTGACTCTAACGAAGTTAGTACTTCCTCTTCTTGGTGAAGGGGAAGAAACTGTGGCGGAT a H N P T G V D P T Q D Q W T E I A S I M K E K N H F P F F D T A Y - b H T T L P V S T L R K I S G L R L L Q S * R R R T T S P S L T P P T - c T Q P Y R C R P Y A R S V D * D C F N H E G E E P L P L L * H R L - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d A C L G V P T S G V C S * H V S I A E I M F S F F W K G K K S V A * - e V C G * R H R G * A L D T S Q S Q K L * S P S S G S G R R Q C R R - f C V V R G T D V R R L I L P S L N S * D H L L L V V E G E K V G G - NsiI | CCAAGGATTTGCCTCTGGCGATCTTGTCAAGGATGCATGGGCCATCCGCTACTTTGTCGATCAGGGCTTTGAGCTTGTTGTTGCTCAGAGTTTCGCCAAG 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 GGTTCCTAAACGGAGACCGCTAGAACAGTTCCTACGTACCCGGTAGGCGATGAAACAGCTAGTCCCGAAACTCGAACAACAACGAGTCTCAAAGCGGTTC a Q G F A S G D L V K D A W A I R Y F V D Q G F E L V V A Q S F A K - b K D L P L A I L S R M H G P S A T L S I R A L S L L L L R V S P R - c P R I C L W R S C Q G C M G H P L L C R S G L * A C C C S E F R Q E - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d W P N A E P S R T L S A H A M R * K T S * P K S S T T A * L K A L - e G L I Q R Q R D Q * P H M P W G S S Q R D P S Q A Q Q Q E S N R W - f V L S K G R A I K D L I C P G D A V K D I L A K L K N N S L T E G L - Bsu36I MfeI BsaI BsrBI | BpmI | | | | | | AACTTTGGTCTCTACGGAGAGCGTGCCGGTTGCTTCCACGCCGTCACATCTCCCGCTCCTGAGGCTTCAAACACAATCACTCGCATTGGCTCTCAATTGG 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 TTGAAACCAGAGATGCCTCTCGCACGGCCAACGAAGGTGCGGCAGTGTAGAGGGCGAGGACTCCGAAGTTTGTGTTAGTGAGCGTAACCGAGAGTTAACC a N F G L Y G E R A G C F H A V T S P A P E A S N T I T R I G S Q L A - b T L V S T E S V P V A S T P S H L P L L R L Q T Q S L A L A L N W - c L W S L R R A C R L L P R R H I S R S * G F K H N H S H W L S I G - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d F K P R * P S R A P Q K W A T V D G A G S A E F V I V R M P E * N - e S S Q D R R L A H R N S G R R * M E R E Q P K L C L * E C Q S E I P - f V K T E V S L T G T A E V G D C R G S R L S * V C D S A N A R L Q - BseRI BglII | NruI EarI PvuI | | PvuI | EarI | | | | | | | | CTATCCTCCAGCGATCGGAGATCTCCAACCCTCCTCTGTACGGTGCCCGTATCGTCAGCACTGTTCTCAACGATCGCGACCTCTTCGCTGAGTGGGAAGA 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 GATAGGAGGTCGCTAGCCTCTAGAGGTTGGGAGGAGACATGCCACGGGCATAGCAGTCGTGACAAGAGTTGCTAGCGCTGGAGAAGCGACTCACCCTTCT a I L Q R S E I S N P P L Y G A R I V S T V L N D R D L F A E W E E - b L S S S D R R S P T L L C T V P V S S A L F S T I A T S S L S G K R - c Y P P A I G D L Q P S S V R C P Y R Q H C S Q R S R P L R * V G R - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d A I R W R D S I E L G G R Y P A R I T L V T R L S R S R K A S H S S - e * G G A I P S R W G E E T R H G Y R * C Q E * R D R G R R Q T P L - f S D E L S R L D G V R R Q V T G T D D A S N E V I A V E E S L P F - EarI SacI BspMI SapI XhoI| FspI | SphI AatII HindIII| BsaI || KpnI DrdII | | | | || | || | | GAACCTGCGCACCATGTCTGGCCGCATCATCAGCATGCGCGACGTCCTCCGAGCCAAGCTTGAAGAGCTCGAGACCCCTGGTACCTGGAACCACATCACC 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 CTTGGACGCGTGGTACAGACCGGCGTAGTAGTCGTACGCGCTGCAGGAGGCTCGGTTCGAACTTCTCGAGCTCTGGGGACCATGGACCTTGGTGTAGTGG a N L R T M S G R I I S M R D V L R A K L E E L E T P G T W N H I T - b T C A P C L A A S S A C A T S S E P S L K S S R P L V P G T T S P - c E P A H H V W P H H Q H A R R P P S Q A * R A R D P W Y L E P H H R - 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 d F R R V M D P R M M L M R S T R R A L S S S S S V G P V Q F W M V - e S G A C W T Q G C * * C A R R G G L W A Q L A R S G Q Y R S G C * - f L V Q A G H R A A D D A H A V D E S G L K F L E L G R T G P V V D G - Bce83I Eco57I ScaI | EcoRI HgiEII | | | | | GACCAGATCGGCATGTTCAGCTTCACTGGCTTGAGCGAGAAGCAAGTACTTCAGCTTCGAGAAGAATTCCACGTCTACATGACCAAGAACGGTCGTATCA 901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1000 CTGGTCTAGCCGTACAAGTCGAAGTGACCGAACTCGCTCTTCGTTCATGAAGTCGAAGCTCTTCTTAAGGTGCAGATGTACTGGTTCTTGCCAGCATAGT a D Q I G M F S F T G L S E K Q V L Q L R E E F H V Y M T K N G R I S - b T R S A C S A S L A * A R S K Y F S F E K N S T S T * P R T V V S - c P D R H V Q L H W L E R E A S T S A S R R I P R L H D Q E R S Y Q - 901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1000 d S W I P M N L K V P K L S F C T S * S R S S N W T * M V L F P R I - e R G S R C T * S * Q S S R S A L V E A E L L I G R R C S W S R D Y * - f V L D A H E A E S A Q A L L L Y K L K S F F E V D V H G L V T T D - BsaI Psp1406I ScaI | | | GCATGGCTGGTCTCAACGAAAACAACGTTGAGTACTTTGCAAAGGCTGTTGATAAGGTTGTTCGTGACGCTGCTTAGATAGACTTTTGAATGGTTGGGAT 1001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1100 CGTACCGACCAGAGTTGCTTTTGTTGCAACTCATGAAACGTTTCCGACAACTATTCCAACAAGCACTGCGACGAATCTATCTGAAAACTTACCAACCCTA a M A G L N E N N V E Y F A K A V D K V V R D A A * I D F * M V G I - b A W L V S T K T T L S T L Q R L L I R L F V T L L R * T F E W L G L - c H G W S Q R K Q R * V L C K G C * * G C S * R C L D R L L N G W D - 1001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1100 d L M A P R L S F L T S Y K A F A T S L T T R S A A * I S K Q I T P I - e C P Q D * R F C R Q T S Q L P Q Q Y P Q E H R Q K S L S K F P Q S - f A H S T E V F V V N L V K C L S N I L N N T V S S L Y V K S H N P - BfiI Tth111I RcaI RcaI | | | | TGTGTACGAACTCATGANAGTGGTCAATTACCTATAGGACCCTGGAAAGAATCGGCGTTTGGGAGATGTTTATTATTACCACACCTTTCATGACTGGGTC 1101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1200 ACACATGCTTGAGTACTNTCACCAGTTAATGGATATCCTGGGACCTTTCTTAGCCGCAAACCCTCTACAAATAATAATGGTGTGGAAAGTACTGACCCAG a V Y E L M ? V V N Y L * D P G K N R R L G D V Y Y Y H T F H D W V - b C T N S * ? W S I T Y R T L E R I G V W E M F I I T T P F M T G S - c C V R T H ? S G Q L P I G P W K E S A F G R C L L L P H L S * L G L - 1101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1200 d T Y S S M ? T T L * R Y S G P F F R R K P S T * * * W V K * S Q T - e Q T R V * S L P * N G I P G Q F S D A N P L H K N N G C R E H S P - f N H V F E H ? H D I V * L V R S L I P T Q S I N I I V V G K M V P D - BsaI | TCTGCAAA 1201 -------- 1208 AGACGTTT a S A - b L Q - c C K - 1201 -------- 1208 d E A F - e R Q L - f R C - Enzymes that do cut: AatII AhdI ApaLI BamHI BbsI Bce83I BcgI BfiI BglII BpmI Bpu10I BsaI BsbI BseRI BspMI BsrBI BssSI Bsu36I DrdII EarI Eco47III Eco57I EcoRI FspI HgiEII HindIII KpnI MfeI NruI NsiI PinAI Psp1406I PvuI RcaI SacI SalI SapI ScaI SphI Tth111I XhoI XmnI Enzymes that do not cut: AceIII AflII AlwNI ApaI ApaBI AscI AvrII BclI BglI BplI Bpu1102I BsaBI BsaXI BsgI BsiWI BsmI BsmBI BspEI BspGI BspLU11I BsrDI BsrGI BssHII Bst1107I BstEII BstXI ClaI DraI DraIII DrdI EagI EciI EcoNI EcoRV FseI HpaI MluI MscI NarI NcoI NdeI NgoAIV NheI NotI NspV PacI Pfl1108I PflMI PmeI PmlI PshAI PstI PvuII RsrII SacII SanDI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI Sse8387I Sse8647I SspI StuI SwaI VspI XbaI XcmI