(Linear) MAP of: ./Contig352.seq check: 7680 from: 1 to: 1160 Contig352 Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * March 20, 2001 16:46 .. SspI BsrGI BglII | | | ccccctccgagttttttttttttttttttggcagaatatttgtagtattacatatcgtgacaagtgtacactgatcctaaGATCTAATACGGATTTCTTT 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 gggggaggctcaaaaaaaaaaaaaaaaaaccgtcttataaacatcataatgtatagcactgttcacatgtgactaggattCTAGATTATGCCTAAAGAAA a P P P S F F F F F L A E Y L * Y Y I S * Q V Y T D P K I * Y G F L L - b P L R V F F F F F W Q N I C S I T Y R D K C T L I L R S N T D F F - c P S E F F F F F F G R I F V V L H I V T S V H * S * D L I R I S F - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d G G L K K K K K K A S Y K Y Y * M D H C T Y V S G L I * Y P N R - e G E S N K K K K K P L I N T T N C I T V L T C Q D * S R I R I E K - f G R R T K K K K K Q C F I Q L I V Y R S L H V S I R L D L V S K K - BsmBI EarI | AatII AatII | | | | TAAGCCTTCGCCTGTCCACCCTTTCCACCAGCGCCCTTCTTATCCAGAAGAGACGTCAACTTTGTCTGGAACGAGTTGACGTCGTGGGTAGTGTACGTCT 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 ATTCGGAAGCGGACAGGTGGGAAAGGTGGTCGCGGGAAGAATAGGTCTTCTCTGCAGTTGAAACAGACCTTGCTCAACTGCAGCACCCATCACATGCAGA a S L R L S T L S T S A L L I Q K R R Q L C L E R V D V V G S V R L - b * A F A C P P F P P A P F L S R R D V N F V W N E L T S W V V Y V S - c K P S P V H P F H Q R P S Y P E E T S T L S G T S * R R G * C T S - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d K L R R R D V R E V L A R R I W F L R * S Q R S R T S T T P L T R R - e L G E G T W G K W W R G E * G S S V D V K D P V L Q R R P Y H V D - f * A K A Q G G K G G A G K K D L L S T L K T Q F S N V D H T T Y T - BsmBI BsrBI BssSI BspGI AceIII BseRI | | | | | | CAGATCGGAGATGCCATGAGCGGGACACTCCAACGGGGTCTGATGCGAACTTGGGTGTCTCGTGCTGGCCCTGGACAGCTCGCTGTGTAATGACCTGACG 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 GTCTAGCCTCTACGGTACTCGCCCTGTGAGGTTGCCCCAGACTACGCTTGAACCCACAGAGCACGACCGGGACCTGTCGAGCGACACATTACTGGACTGC a R S E M P * A G H S N G V * C E L G C L V L A L D S S L C N D L T - b D R R C H E R D T P T G S D A N L G V S C W P W T A R C V M T * R - c Q I G D A M S G T L Q R G L M R T W V S R A G P G Q L A V * * P D G - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d L D S I G H A P C E L P T Q H S S P H R T S A R S L E S H L S R V - e * I P S A M L P V S W R P R I R V Q T D R A P G P C S A T Y H G S - f E S R L H W S R S V G V P D S A F K P T E H Q G Q V A R Q T I V Q R - AflII BsmI BglI ApaI| BcgI | | || | | GATGGCCTCCTCGGCTTCGGCAACGATAGGGCCCTTAAGCTCCTTGCCTTCGGCTCTGGCCCTTCCTTGGAGGTACTGCTCGGCGGCATTCTTGGCATCG 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 CTACCGGAGGAGCCGAAGCCGTTGCTATCCCGGGAATTCGAGGAACGGAAGCCGAGACCGGGAAGGAACCTCCATGACGAGCCGCCGTAAGAACCGTAGC a D G L L G F G N D R A L K L L A F G S G P S L E V L L G G I L G I G - b M A S S A S A T I G P L S S L P S A L A L P W R Y C S A A F L A S - c W P P R L R Q R * G P * A P C L R L W P F L G G T A R R H S W H R - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d S P R R P K P L S L A R L S R A K P E P G E K S T S S P P M R P M - e P H G G R S R C R Y P G * A G Q R R S Q G K R P P V A R R C E Q C R - f I A E E A E A V I P G K L E K G E A R A R G Q L Y Q E A A N K A D - NgoAIV BsaXI BcgI | BsaI | Tth111I BpmI | | | | | | GCATCGGCGAAGAAGCGAATACCGCCGGCCTCGGTCTCGGAGGCGTAGTATTTGGGCTGGAAGGGTTCTGGAGCACCGACGTGGTCTGCTGTGCCGAGAA 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 CGTAGCCGCTTCTTCGCTTATGGCGGCCGGAGCCAGAGCCTCCGCATCATAAACCCGACCTTCCCAAGACCTCGTGGCTGCACCAGACGACACGGCTCTT a I G E E A N T A G L G L G G V V F G L E G F W S T D V V C C A E N - b A S A K K R I P P A S V S E A * Y L G W K G S G A P T W S A V P R T - c H R R R S E Y R R P R S R R R S I W A G R V L E H R R G L L C R E - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d P M P S S A F V A P R P R P P T T N P S S P N Q L V S T T Q Q A S F - e C R R L L S Y R R G R D R L R L I Q A P L T R S C R R P R S H R S - f A D A F F R I G G A E T E S A Y Y K P Q F P E P A G V H D A T G L - AlwNI Bpu10I PvuII AlwNI XhoI | | | | CGCTGAGGTTCTGCATAACAGCAGCTGACTTTCCAGCAGCAGTGCCTGGTGCGACaggggcAAATTGGTTGACGGACTCGAGTGTGAAGGATGGTGTGTA 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 GCGACTCCAAGACGTATTGTCGTCGACTGAAAGGTCGTCGTCACGGACCACGCTGtccccgTTTAACCAACTGCCTGAGCTCACACTTCCTACCACACAT a A E V L H N S S * L S S S S A W C D R G K L V D G L E C E G W C V - b L R F C I T A A D F P A A V P G A T G A N W L T D S S V K D G V Y - c R * G S A * Q Q L T F Q Q Q C L V R Q G Q I G * R T R V * R M V C T - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d A S T R C L L L Q S E L L L A Q H S L P L N T S P S S H S P H H T - e R Q P E A Y C C S V K W C C H R T R C P C I P Q R V R T H L I T H - f V S L N Q M V A A S K G A A T G P A V P A F Q N V S E L T F S P T Y - BcgI BcgI SphI BsmI XmnI BamHI EcoRI | | | | | | CTCGGAGCGCATGCCGAATCGAATGCTATCGTCGAAAGACTTCTCATCGGGATCCATGTTGTTGAATTCATTTCGGTCCTTGTTTCTATccttgtcGTCA 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 GAGCCTCGCGTACGGCTTAGCTTACGATAGCAGCTTTCTGAAGAGTAGCCCTAGGTACAACAACTTAAGTAAAGCCAGGAACAAAGATAggaacagCAGT a L G A H A E S N A I V E R L L I G I H V V E F I S V L V S I L V V I - b S E R M P N R M L S S K D F S S G S M L L N S F R S L F L S L S S - c R S A C R I E C Y R R K T S H R D P C C * I H F G P C F Y P C R H - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d S P A C A S D F A I T S L S R M P I W T T S N M E T R T E I R T T - e V R L A H R I S H * R R F V E * R S G H Q Q I * K P G Q K * G Q R * - f E S R M G F R I S D D F S K E D P D M N N F E N R D K N R D K D D - BseRI BssSI DrdI SacII NarI EciI | | | | | | TCATCccTTCGTCGATCacctTTGCCTCctcgtggaccTCGACCACGACCACGACCTCCACGTCCACCGCGGCCtCTGGCGCCAAAGCCGCCTCCGCCTC 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 AGTAGggAAGCAGCTAGtggaAACGGAGgagcacctggAGCTGGTGCTGGTGCTGGAGGTGCAGGTGGCGCCGGaGACCGCGGTTTCGGCGGAGGCGGAG a I P S S I T F A S S W T S T T T T T S T S T A A S G A K A A S A S - b S S L R R S P L P P R G P R P R P R P P R P P R P L A P K P P P P R - c H P F V D H L C L L V D L D H D H D L H V H R G L W R Q S R L R L - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d M M G E D I V K A E E H V E V V V V V E V D V A A E P A L A A E A E - e * G K T S * R Q R R T S R S W S W S R W T W R P R Q R W L R R R R - f D D R R R D G K G G R P G R G R G R G G R G G R G R A G F G G G G - AhdI MscI Eco57I EciI | | | | GACCTCTTGTCATAGGCTGGCCAGTTCGCATAGGCGAGGATGGGCGATCCTGGGCTGAAGATCCTCCCCTACCCGCAAATCCGCCTCGTCCACGGAAACT 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 CTGGAGAACAGTATCCGACCGGTCAAGCGTATCCGCTCCTACCCGCTAGGACCCGACTTCTAGGAGGGGATGGGCGTTTAGGCGGAGCAGGTGCCTTTGA a T S C H R L A S S H R R G W A I L G * R S S P T R K S A S S T E T - b P L V I G W P V R I G E D G R S W A E D P P L P A N P P R P R K L - c D L L S * A G Q F A * A R M G D P G L K I L P Y P Q I R L V H G N Y - 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 d V E Q * L S A L E C L R P H A I R P Q L D E G V R L D A E D V S V - e S R K D Y A P W N A Y A L I P S G P S F I R G * G C I R R T W P F - f R G R T M P Q G T R M P S S P R D Q A S S G G R G A F G G R G R F S - BsrBI BseRI Bpu10I | NgoAIV | | | | ACCACGACCCCTTAGACCTCCTCTGCCACGCAGGGGCAGACTCTTTATGTCGATGACCTTGGGACCACCAGCCTGAGCGGGACGGGCAGCCGGCCTCGGG 901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1000 TGGTGCTGGGGAATCTGGAGGAGACGGTGCGTCCCCGTCTGAGAAATACAGCTACTGGAACCCTGGTGGTCGGACTCGCCCTGCCCGTCGGCCGGAGCCC a T T T P * T S S A T Q G Q T L Y V D D L G T T S L S G T G S R P R G - b P R P L R P P L P R R G R L F M S M T L G P P A * A G R A A G L G - c H D P L D L L C H A G A D S L C R * P W D H Q P E R D G Q P A S G - 901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1000 d V V V G * V E E A V C P C V R * T S S R P V V L R L P V P L R G R - e * W S G K S R R Q W A P A S E K H R H G Q S W W G S R S P C G A E P - f G R G R L G G R G R L P L S K I D I V K P G G A Q A P R A A P R P - BplI BsgI StuI|XhoI SacI || | | GTGCTGGGACCATCGGCGGCCTGAGTCTGTGAAGTCGAGAATTGGCGGGCGTAGTTTCGGAGAGCATCCGAGGCCTGCTCGAGCTCTGGCCTTGTCCGGC 1001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1100 CACGACCCTGGTAGCCGCCGGACTCAGACACTTCAGCTCTTAACCGCCCGCATCAAAGCCTCTCGTAGGCTCCGGACGAGCTCGAGACCGGAACAGGCCG a A G T I G G L S L * S R E L A G V V S E S I R G L L E L W P C P A - b V L G P S A A * V C E V E N W R A * F R R A S E A C S S S G L V R L - c C W D H R R P E S V K S R I G G R S F G E H P R P A R A L A L S G - 1001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1100 d P A P V M P P R L R H L R S N A P T T E S L M R P R S S S Q G Q G A - e H Q S W R R G S D T F D L I P P R L K P S C G L G A R A R A K D P - f T S P G D A A Q T Q S T S F Q R A Y N R L A D S A Q E L E P R T R - BssSI EcoRI | | TGCACACCGAACGGAAGGCGTGGCGTTGCATATTGGCGGTGGTTGCCTCGTGCCgaattc 1101 ---------+---------+---------+---------+---------+---------+ 1160 ACGTGTGGCTTGCCTTCCGCACCGCAACGTATAACCGCCACCAACGGAGCACGGcttaag a A H R T E G V A L H I G G G C L V P N - b H T E R K A W R C I L A V V A S C R I - c C T P N G R R G V A Y W R W L P R A E F - 1101 ---------+---------+---------+---------+---------+---------+ 1160 d A C R V S P T A N C I P P P Q R T G F E - e Q V G F P L R P T A Y Q R H N G R A S N - f S C V S R F A H R Q M N A T T A E H R I - Enzymes that do cut: AatII AceIII AflII AhdI AlwNI ApaI BamHI BcgI BglI BglII BplI BpmI Bpu10I BsaI BsaXI BseRI BsgI BsmI BsmBI BspGI BsrBI BsrGI BssSI DrdI EarI EciI Eco57I EcoRI MscI NarI NgoAIV PvuII SacI SacII SphI SspI StuI Tth111I XhoI XmnI Enzymes that do not cut: ApaBI ApaLI AscI AvrII BbsI Bce83I BclI BfiI Bpu1102I BsaBI BsbI BsiWI BspEI BspLU11I BspMI BsrDI BssHII Bst1107I BstEII BstXI Bsu36I ClaI DraI DraIII DrdII EagI Eco47III EcoNI EcoRV FseI FspI HgiEII HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI NotI NruI NsiI NspV PacI Pfl1108I PflMI PinAI PmeI PmlI PshAI Psp1406I PstI PvuI RcaI RsrII SalI SanDI SapI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI Sse8387I Sse8647I SwaI VspI XbaI XcmI