(Linear) MAP of: ./Contig375.seq check: 3166 from: 1 to: 1117 Contig375 Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * March 20, 2001 16:46 .. Eco57I BspLU11I ApaI RcaI | XmnI BsmI | | | | | | | gggcccccccctccgagtttttttttttttttttatcctgaaggttcattgaatcatgaggtTGAAAGTTttcCCCATCAACCACGAATgcttacATGTG 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 cccggggggggaggctcaaaaaaaaaaaaaaaaataggacttccaagtaacttagtactccaACTTTCAAaagGGGTAGTTGGTGCTTAcgaatgTACAC a G P P P P S F F F F F Y P E G S L N H E V E S F P H Q P R M L T C V - b G P P L R V F F F F F I L K V H * I M R L K V F P I N H E C L H V - c A P P S E F F F F F L S * R F I E S * G * K F S P S T T N A Y M C - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d A G G E S N K K K K K D Q L N M S D H P Q F N E G D V V F A * M H - e P G G R R T K K K K K I R F T * Q I M L N F T K G M L W S H K C T - f G G G G L K K K K K * G S P E N F * S T S L K G W * G R I S V H - BglI | TCCCCCTAGCTAAACAACGCACACAAGATCGAAACAGCAGCAACGTCCACCTTTTTACTGAGCCTTCTTGGCAAACTCCACCCGGAGAATGAGATGCTTG 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 AGGGGGATCGATTTGTTGCGTGTGTTCTAGCTTTGTCGTCGTTGCAGGTGGAAAAATGACTCGGAAGAACCGTTTGAGGTGGGCCTCTTACTCTACGAAC a P L A K Q R T Q D R N S S N V H L F T E P S W Q T P P G E * D A * - b S P * L N N A H K I E T A A T S T F L L S L L G K L H P E N E M L E - c P P S * T T H T R S K Q Q Q R P P F Y * A F L A N S T R R M R C L - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d G G L * V V C V L D F C C C R G G K * Q A K K A F E V R L I L H K - e D G * S F L A C L I S V A A V D V K K S L R R P L S W G S F S I S - f T G R A L C R V C S R F L L L T W R K V S G E Q C V G G P S H S A Q - Tth111I BglI | RsrII MscI | | | | AAACCAAATCCGTCCATCTTCGCACAAGCCTTGACGGCGTCGCCTCGGTCCGCAAAGCTGATAAAGGCGAAACCCTTGGCCATGCCAGTCTCTCTGTCCT 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 TTTGGTTTAGGCAGGTAGAAGCGTGTTCGGAACTGCCGCAGCGGAGCCAGGCGTTTCGACTATTTCCGCTTTGGGAACCGGTACGGTCAGAGAGACAGGA a N Q I R P S S H K P * R R R L G P Q S * * R R N P W P C Q S L C P - b T K S V H L R T S L D G V A S V R K A D K G E T L G H A S L S V L - c K P N P S I F A Q A L T A S P R S A K L I K A K P L A M P V S L S L - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d F G F G D M K A C A K V A D G R D A F S I F A F G K A M G T E R D - e S V L D T W R R V L R S P T A E T R L A S L P S V R P W A L R E T R - f F W I R G D E C L G Q R R R R P G C L Q Y L R F G Q G H W D R Q G - BglI MscI BseRI AceIII| Psp1406I DraIII | | || | | TGGCCAAGAACACTCGGGTGACACGACCGAAACGCTCGAACATATCCCGCAGCTCTGCCTCCTCGGCCATCTCGGAAACGTTGGTGACACGCAGTGTAGC 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 ACCGGTTCTTGTGAGCCCACTGTGCTGGCTTTGCGAGCTTGTATAGGGCGTCGAGACGGAGGAGCCGGTAGAGCCTTTGCAACCACTGTGCGTCACATCG a W P R T L G * H D R N A R T Y P A A L P P R P S R K R W * H A V * Q - b G Q E H S G D T T E T L E H I P Q L C L L G H L G N V G D T Q C S - c A K N T R V T R P K R S N I S R S S A S S A I S E T L V T R S V A - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d K A L F V R T V R G F R E F M D R L E A E E A M E S V N T V R L T A - e P W S C E P S V V S V S S C I G C S Q R R P W R P F T P S V C H L - f Q G L V S P H C S R F A R V Y G A A R G G R G D R F R Q H C A T Y - BamHI NdeI | | AAAGTCGTCCCTCTCGCCAAACTTGGATCCCATTCTCTCGCCAGCACTTCCGTTGCCACGCAGGGCAGGGGGAACATATGAACCCTTCTTGGCACCAGCA 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 TTTCAGCAGGGAGAGCGGTTTGAACCTAGGGTAAGAGAGCGGTCGTGAAGGCAACGGTGCGTCCCGTCCCCCTTGTATACTTGGGAAGAACCGTGGTCGT a S R P S R Q T W I P F S R Q H F R C H A G Q G E H M N P S W H Q Q - b K V V P L A K L G S H S L A S T S V A T Q G R G N I * T L L G T S S - c K S S L S P N L D P I L S P A L P L P R R A G G T Y E P F L A P A - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d F D D R E G F K S G M R E G A S G N G R L A P P V Y S G K K A G A - e L T T G R A L S P D W E R A L V E T A V C P L P F M H V R R P V L - f C L R G E R W V Q I G N E R W C K R Q W A P C P S C I F G E Q C W C - BglI BsmBI BcgI NcoI SacII | BcgI |Eco57I BsrBI | NarI | | | | | | | | | | GCCGCGGCGGCTGAAGGCTCGTCTCCCATACCAGCAGCGACATCGGCAGCGCCGCTCTCTCCAATGGGCGCCATGGTGTCCTTGTAAGGACATCGGGCAG 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 CGGCGCCGCCGACTTCCGAGCAGAGGGTATGGTCGTCGCTGTAGCCGTCGCGGCGAGAGAGGTTACCCGCGGTACCACAGGAACATTCCTGTAGCCCGTC a P R R L K A R L P Y Q Q R H R Q R R S L Q W A P W C P C K D I G Q - b R G G * R L V S H T S S D I G S A A L S N G R H G V L V R T S G S - c A A A A E G S S P I P A A T S A A P L S P M G A M V S L * G H R A V - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d A A A A S P E D G M G A A V D A A G S E G I P A M T D K Y P C R A - e L R P P Q L S T E W V L L S M P L A A R E L P R W P T R T L V D P L - f G R R S F A R R G Y W C R C R C R R E R W H A G H H G Q L S M P C - BspMI DrdI | AceIII | | Bce83I | | | | TGAAATGCTGACCGTTGCAAATACGACACTTGACCTGCTTGTCCTTGAGCTGGTCCTTCATGGTCTGGGTAGCAGTGTCGGCGGTATCGTCCTTCTCGGC 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 ACTTTACGACTGGCAACGTTTATGCTGTGAACTGGACGAACAGGAACTCGACCAGGAAGTACCAGACCCATCGTCACAGCCGCCATAGCAGGAAGAGCCG a * N A D R C K Y D T * P A C P * A G P S W S G * Q C R R Y R P S R P - b E M L T V A N T T L D L L V L E L V L H G L G S S V G G I V L L G - c K C * P L Q I R H L T C L S L S W S F M V W V A V S A V S S F S A - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d T F H Q G N C I R C K V Q K D K L Q D K M T Q T A T D A T D D K E A - e S I S V T A F V V S S R S T R S S T R * P R P L L T P P I T R R P - f H F A S R Q L Y S V Q G A Q G Q A P G E H D P Y C H R R Y R G E R - AhdI XcmI | HgiEII AlwNI BamHI | | | | | CTTTCGCCAGTTGACGCTTGGTCGGAAGATGATGTTCTCACCGACAGAGGTGGTGTCGGGGGCAGGGCCTGGGGGATCCTTTGCACTGAGGCCGAACTTT 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 GAAAGCGGTCAACTGCGAACCAGCCTTCTACTACAAGAGTGGCTGTCTCCACCACAGCCCCCGTCCCGGACCCCCTAGGAAACGTGACTCCGGCTTGAAA a F A S * R L V G R * C S H R Q R W C R G Q G L G D P L H * G R T L - b L S P V D A W S E D D V L T D R G G V G G R A W G I L C T E A E L W - c F R Q L T L G R K M M F S P T E V V S G A G P G G S F A L R P N F - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d K R W N V S P R F I I N E G V S T T D P A P G P P D K A S L G F K - e R E G T S A Q D S S S T R V S L P P T P P L A Q P I R Q V S A S S - f G K A L Q R K T P L H H E * R C L H H R P C P R P S G K C Q P R V K - MscI BsaI EagI Pfl1108I | | | | GGCCAGGTTTTTCGGTCGGCGACGCGGGGGTTTACGGTCTCGGTGTGGGTAATGAATCGGACGCGGCGAACGGTCTTGACCTTGCGGCCGTCGTCATCGT 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 CCGGTCCAAAAAGCCAGCCGCTGCGCCCCCAAATGCCAGAGCCACACCCATTACTTAGCCTGCGCCGCTTGCCAGAACTGGAACGCCGGCAGCAGTAGCA a A R F F G R R R G G L R S R C G * * I G R G E R S * P C G R R H R - b P G F S V G D A G V Y G L G V G N E S D A A N G L D L A A V V I V - c G Q V F R S A T R G F T V S V W V M N R T R R T V L T L R P S S S * - 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 d P W T K R D A V R P N V T E T H T I F R V R R V T K V K R G D D D - e Q G P K E T P S A P T * P R P T P L S D S A A F P R S R A A T T M T - f A L N K P R R R P P K R D R H P Y H I P R P S R D Q G Q P R R * R - DrdI AatII | Tth111I| | BsaI || | Bpu10I BseRI BplI | || | BfiI | | | | || | | AGCGGAATGTGATGATGGTCTTTGTTCCATCCTTGTTGGCGATGGTCTGAGGCTCAGGGAGATCGGTGGAGGTCTCCTCGACGTCGTCGTCGTCGGCCCA 901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1000 TCGCCTTACACTACTACCAGAAACAAGGTAGGAACAACCGCTACCAGACTCCGAGTCCCTCTAGCCACCTCCAGAGGAGCTGCAGCAGCAGCAGCCGGGT a S G M * * W S L F H P C W R W S E A Q G D R W R S P R R R R R R P S - b A E C D D G L C S I L V G D G L R L R E I G G G L L D V V V V G P - c R N V M M V F V P S L L A M V * G S G R S V E V S S T S S S S A Q - 901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1000 d Y R F T I I T K T G D K N A I T Q P E P L D T S T E E V D D D D A W - e A S H S S P R Q E M R T P S P R L S L S I P P P R R S T T T T P G - f L P I H H H D K N W G Q Q R H D S A * P S R H L D G R R R R R R G - DrdII BsaI | | GTCGTGCTTGGGCTGGTTCGCAACTGCGGCAGCCATGATGAATCGAGCGTATCCGTGCGACTTTTTGAGTTATAGGGGTGTAGGTCtCGCGGTAAGGTCG 1001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1100 CAGCACGAACCCGACCAAGCGTTGACGCCGTCGGTACTACTTAGCTCGCATAGGCACGCTGAAAAACTCAATATCCCCACATCCAGaGCGCCATTCCAGC a R A W A G S Q L R Q P * * I E R I R A T F * V I G V * V S R * G R - b V V L G L V R N C G S H D E S S V S V R L F E L * G C R S R G K V G - c S C L G W F A T A A A M M N R A Y P C D F L S Y R G V G L A V R S - 1001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1100 d D H K P Q N A V A A A M I F R A Y G H S K K L * L P T P R A T L D - e T T S P S T R L Q P L W S S D L T D T R S K S N Y P H L D R P L T - f L R A Q A P E C S R C G H H I S R I R A V K Q T I P T Y T E R Y P R - BssSI EcoRI | | GGcctcgtgccgaattc 1101 ---------+------- 1117 CCggagcacggcttaag a A S C R I - b P R A E F - c G L V P N - 1101 ---------+------- 1117 d P R T G F E - e P G R A S N - f A E H R I - Enzymes that do cut: AatII AceIII AhdI AlwNI ApaI BamHI Bce83I BcgI BfiI BglI BplI Bpu10I BsaI BseRI BsmI BsmBI BspLU11I BspMI BsrBI BssSI DraIII DrdI DrdII EagI Eco57I EcoRI HgiEII MscI NarI NcoI NdeI Pfl1108I Psp1406I RcaI RsrII SacII Tth111I XcmI XmnI Enzymes that do not cut: AflII ApaBI ApaLI AscI AvrII BbsI BclI BglII BpmI Bpu1102I BsaBI BsaXI BsbI BsgI BsiWI BspEI BspGI BsrDI BsrGI BssHII Bst1107I BstEII BstXI Bsu36I ClaI DraI EarI EciI Eco47III EcoNI EcoRV FseI FspI HindIII HpaI KpnI MfeI MluI NgoAIV NheI NotI NruI NsiI NspV PacI PflMI PinAI PmeI PmlI PshAI PstI PvuI PvuII SacI SalI SanDI SapI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI Sse8387I Sse8647I SspI StuI SwaI VspI XbaI XhoI