(Linear) MAP of: ./Contig540.seq check: 5681 from: 1 to: 1253 Contig540 Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * March 20, 2001 16:47 .. XhoI KpnI ApaI | SalI BsmBI | | | | | ccccttgggtaccgggcccccctcgagtttttTTTTTTTTTTTTTAAGTCTCTTAACTTCTTTTTAACTACAAAGTCGACAAATACACGTCTCCCCTTAG 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 ggggaacccatggcccggggggagctcaaaaaAAAAAAAAAAAAATTCAGAGAATTGAAGAAAAATTGATGTTTCAGCTGTTTATGTGCAGAGGGGAATC a P L G Y R A P L E F F F F F F K S L N F F L T T K S T N T R L P L E - b P L G T G P P S S F F F F F L S L L T S F * L Q S R Q I H V S P * - c P W V P G P P R V F F F F F * V S * L L F N Y K V D K Y T S P L R - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d R P Y R A G R S N K K K K K L D R L K K K V V F D V F V R R G K - e G Q T G P G G R T K K K K K * T E * S R K L * L T S L Y V D G R L - f G K P V P G G E L K K K K K K L R K V E K * S C L R C I C T E G * - EciI BsaI | EarI BsaBI BsmBI | | | | | AGCCTTCCTTTTCAAGACTCGGCGGAGACCAACCTCTCGCTAGTGATGTCACCTTCTCTTCCCAAAGGATATATATCGCAACTCTTACTACAAAGGAGAC 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 TCGGAAGGAAAAGTTCTGAGCCGCCTCTGGTTGGAGAGCGATCACTACAGTGGAAGAGAAGGGTTTCCTATATATAGCGTTGAGAATGATGTTTCCTCTG a P S F S R L G G D Q P L A S D V T F S S Q R I Y I A T L T T K E T - b S L P F Q D S A E T N L S L V M S P S L P K G Y I S Q L L L Q R R R - c A F L F K T R R R P T S R * * C H L L F P K D I Y R N S Y Y K G D - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d S G E K E L S P P S W G R A L S T V K E E W L I Y I A V R V V F S V - e A K R K L V R R L G V E R * H H * R R K G F S I Y R L E * * L P S - f L R G K * S E A S V L R E S T I D G E R G L P Y I D C S K S C L L - SphI NsiI | BsaXI BfiI BssSI Psp1406I | | | | | | GCTGCCCTGCGTAGAATCATGCATGCCAAGTTTCCCAACAAGCTAACAAAAACTCCTTCCCAGTCCTCGTACTCATCCTCGTGTCCGTAAACGTTTAGCT 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 CGACGGGACGCATCTTAGTACGTACGGTTCAAAGGGTTGTTCGATTGTTTTTGAGGAAGGGTCAGGAGCATGAGTAGGAGCACAGGCATTTGCAAATCGA a L P C V E S C M P S F P T S * Q K L L P S P R T H P R V R K R L A - b C P A * N H A C Q V S Q Q A N K N S F P V L V L I L V S V N V * L - c A A L R R I M H A K F P N K L T K T P S Q S S Y S S S C P * T F S L - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d S G Q T S D H M G L K G V L * C F S R G L G R V * G R T R L R K A - e A A R R L I M C A L N G L L S V F V G E W D E Y E D E H G Y V N L - f R Q G A Y F * A H W T E W C A L L F E K G T R T S M R T D T F T * S - BspGI EcoNI | | | TAGTATGTACGCTTGAAAGGTGGCACGGGCTTGCACTCGGCCTCGTCCAGGGTGGTGCCGATGACCTTTCGGTAGGACAGATCGACCTTGCCGTGAGGCT 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 ATCATACATGCGAACTTTCCACCGTGCCCGAACGTGAGCCGGAGCAGGTCCCACCACGGCTACTGGAAAGCCATCCTGTCTAGCTGGAACGGCACTCCGA a * Y V R L K G G T G L H S A S S R V V P M T F R * D R S T L P * G C - b S M Y A * K V A R A C T R P R P G W C R * P F G R T D R P C R E A - c V C T L E R W H G L A L G L V Q G G A D D L S V G Q I D L A V R L - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d * Y T R K F P P V P K C E A E D L T T G I V K R Y S L D V K G H P - e K T H V S S L H C P S A S P R T W P P A S S R E T P C I S R A T L S - f L I Y A Q F T A R A Q V R G R G P H H R H G K P L V S R G Q R S A - BssSI | GCTTCTGGAAAGAGAGTGTGTGCTTCATCCAGTTCTCGTCATCTCGGTCGGGGAAATCCTCGCGGGCGTGAGCACCACGAGACTCCTTTCGGTTGGCGGC 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 CGAAGACCTTTCTCTCACACACGAAGTAGGTCAAGAGCAGTAGAGCCAGCCCCTTTAGGAGCGCCCGCACTCGTGGTGCTCTGAGGAAAGCCAACCGCCG a F W K E S V C F I Q F S S S R S G K S S R A * A P R D S F R L A A - b A S G K R V C A S S S S R H L G R G N P R G R E H H E T P F G W R Q - c L L E R E C V L H P V L V I S V G E I L A G V S T T R L L S V G G - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d Q K Q F S L T H K M W N E D D R D P F D E R A H A G R S E K R N A A - e S R S L S H T S * G T R T M E T P S I R A P T L V V L S R E T P P - f A E P F L T H A E D L E R * R P R P F G R P R S C W S V G K P Q R - Bce83I NspV BglI | | | AGACTCGGCGGTTTGGATAGCGCAAGTCAGTAGGTTTCGAAGCTCAAGAGTCTCAACGAGATCAGAGTTCCAGATCATGCTGCGGTCCTTCATGCCAACC 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 TCTGAGCCGCCAAACCTATCGCGTTCAGTCATCCAAAGCTTCGAGTTCTCAGAGTTGCTCTAGTCTCAAGGTCTAGTACGACGCCAGGAAGTACGGTTGG a D S A V W I A Q V S R F R S S R V S T R S E F Q I M L R S F M P T - b T R R F G * R K S V G F E A Q E S Q R D Q S S R S C C G P S C Q P - c R L G G L D S A S Q * V S K L K S L N E I R V P D H A A V L H A N L - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d S E A T Q I A C T L L N R L E L T E V L D S N W I M S R D K M G V - e L S P P K S L A L * Y T E F S L L R L S I L T G S * A A T R * A L - f C V R R N P Y R L D T P K S A * S D * R S * L E L D H Q P G E H W G - BspGI BsrGI ClaI DrdI | PshAI | | | | | TGGCTGTACATGCCATCGATCTCCTTCATCTTGCGGACACCCTCGTCCAGACTCTCCTGGGTTCGGAACACGCTGACCTCGGTCTGCATAGCCTTCTGCA 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 ACCGACATGTACGGTAGCTAGAGGAAGTAGAACGCCTGTGGGAGCAGGTCTGAGAGGACCCAAGCCTTGTGCGACTGGAGCCAGACGTATCGGAAGACGT a W L Y M P S I S F I L R T P S S R L S W V R N T L T S V C I A F C M - b G C T C H R S P S S C G H P R P D S P G F G T R * P R S A * P S A - c A V H A I D L L H L A D T L V Q T L L G S E H A D L G L H S L L H - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d Q S Y M G D I E K M K R V G E D L S E Q T R F V S V E T Q M A K Q - e R A T C A M S R R * R A S V R T W V R R P E S C A S R P R C L R R C - f P Q V H W R D G E D Q P C G R G S E G P N P V R Q G R D A Y G E A - EcoNI Tth111I | BsaI Eco57I | | | | TGGCAAGTCGGATCTCAGCAGTGCTCTTGGGGCCGTCGGCGGTTCGGACCTTGTCGAGGGTCTCGATGTGGTCAGAAGCAGCGTCAGCAGCGGTGGGCTT 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 ACCGTTCAGCCTAGAGTCGTCACGAGAACCCCGGCAGCCGCCAAGCCTGGAACAGCTCCCAGAGCTACACCAGTCTTCGTCGCAGTCGTCGCCACCCGAA a A S R I S A V L L G P S A V R T L S R V S M W S E A A S A A V G F - b W Q V G S Q Q C S W G R R R F G P C R G S R C G Q K Q R Q Q R W A S - c G K S D L S S A L G A V G G S D L V E G L D V V R S S V S S G G L - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d M A L R I E A T S K P G D A T R V K D L T E I H D S A A D A A T P K - e P L D S R L L A R P A T P P E S R T S P R S T T L L L T L L P P S - f H C T P D * C H E Q P R R R N P G Q R P D R H P * F C R * C R H A - BcgI NruI BcgI EarI | | | | CAGAGCATCACCGGGGTTGAACTTGTCGCGAATGGTGTGCGAAACGGCACGACCAAAGACAACAAGATCGAGAAGAGAGTTGGCACCCAGACGGTTAGCA 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 GTCTCGTAGTGGCCCCAACTTGAACAGCGCTTACCACACGCTTTGCCGTGCTGGTTTCTGTTGTTCTAGCTCTTCTCTCAACCGTGGGTCTGCCAATCGT a R A S P G L N L S R M V C E T A R P K T T R S R R E L A P R R L A - b E H H R G * T C R E W C A K R H D Q R Q Q D R E E S W H P D G * H - c Q S I T G V E L V A N G V R N G T T K D N K I E K R V G T Q T V S T - 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 d L A D G P N F K D R I T H S V A R G F V V L D L L S N A G L R N A - e * L M V P T S S T A F P T R F P V V L S L L I S F L T P V W V T L - f E S C * R P Q V Q R S H H A F R C S W L C C S R S S L Q C G S P * C - CCGTGGACGGACACACAAGCGGCCTCACCACAAGCGTACAGACCAGGAACAACCTTGTCCTCACCCTTCTCATCAACGGTAAGAACCTCACCAGTGTATC 901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1000 GGCACCTGCCTGTGTGTTCGCCGGAGTGGTGTTCGCATGTCTGGTCCTTGTTGGAACAGGAGTGGGAAGAGTAGTTGCCATTCTTGGAGTGGTCACATAG a P W T D T Q A A S P Q A Y R P G T T L S S P F S S T V R T S P V Y R - b R G R T H K R P H H K R T D Q E Q P C P H P S H Q R * E P H Q C I - c V D G H T S G L T T S V Q T R N N L V L T L L I N G K N L T S V S - 901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1000 d G H V S V C A A E G C A Y L G P V V K D E G K E D V T L V E G T Y - e V T S P C V L P R V V L T C V L F L R T R V R R M L P L F R V L T D - f R P R V C L R G * W L R V S W S C G Q G * G E * * R Y S G * W H I - BsaI | GTGTAGGGATACCACCCATGTTGTAGTGGACGGTGGGGAGAACGGGAATAGGCTGCTTGCGGACATCGACACCAGCGAAAATACCAGCGGTCTCAGAGAT 1001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1100 CACATCCCTATGGTGGGTACAACATCACCTGCCACCCCTCTTGCCCTTATCCGACGAACGCCTGTAGCTGTGGTCGCTTTTATGGTCGCCAGAGTCTCTA a V G I P P M L * W T V G R T G I G C L R T S T P A K I P A V S E I - b V * G Y H P C C S G R W G E R E * A A C G H R H Q R K Y Q R S Q R Y - c C R D T T H V V V D G G E N G N R L L A D I D T S E N T S G L R D - 1001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1100 d R T P I G G M N Y H V T P L V P I P Q K R V D V G A F I G A T E S I - e H L S V V W T T T S P P S F P F L S S A S M S V L S F V L P R L S - f T Y P Y W G H Q L P R H P S R S Y A A Q P C R C W R F Y W R D * L - Bpu1102I AceIII Bpu1102I BpmI BsbI NcoI | | | | | | ACCGGGCAGACGCTCAGCAAGGATCTCAGCGGGGAGATGGCTGAGCTGGAGGTAGATGTGATCCTTCTCGGCACCAACACCACGGCCCTCACGGATCTCC 1101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1200 TGGCCCGTCTGCGAGTCGTTCCTAGAGTCGCCCCTCTACCGACTCGACCTCCATCTACACTAGGAAGAGCCGTGGTTGTGGTGCCGGGAGTGCCTAGAGG a P G R R S A R I S A G R W L S W R * M * S F S A P T P R P S R I S - b R A D A Q Q G S Q R G D G * A G G R C D P S R H Q H H G P H G S P - c T G Q T L S K D L S G E M A E L E V D V I L L G T N T T A L T D L H - 1101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1200 d G P L R E A L I E A P L H S L Q L Y I H D K E A G V G R G E R I E - e V P C V S L L S R L P S I A S S S T S T I R R P V L V V A R V S R - f Y R A S A * C P D * R P S P Q A P P L H S G E R C W C W P G * P D G - BstXI BssSI BssSI EcoRI | | | | ATGGTCATGGATCGGGAAACGACATCACGAGAAGCAAGCCTCGTGCCGAATTC 1201 ---------+---------+---------+---------+---------+--- 1253 TACCAGTACCTAGCCCTTTGCTGTAGTGCTCTTCGTTCGGAGCACGGCTTAAG a M V M D R E T T S R E A S L V P N - b W S W I G K R H H E K Q A S C R I - c G H G S G N D I T R S K P R A E F - 1201 ---------+---------+---------+---------+---------+--- 1253 d M T M S R S V V D R S A L R T G F E - e W P * P D P F S M V L L L G R A S N - f H D H I P F R C * S F C A E H R I - Enzymes that do cut: AceIII ApaI Bce83I BcgI BfiI BglI BpmI Bpu1102I BsaI BsaBI BsaXI BsbI BsmBI BspGI BsrGI BssSI BstXI ClaI DrdI EarI EciI Eco57I EcoNI EcoRI KpnI NcoI NruI NsiI NspV PshAI Psp1406I SalI SphI Tth111I XhoI Enzymes that do not cut: AatII AflII AhdI AlwNI ApaBI ApaLI AscI AvrII BamHI BbsI BclI BglII BplI Bpu10I BseRI BsgI BsiWI BsmI BspEI BspLU11I BspMI BsrBI BsrDI BssHII Bst1107I BstEII Bsu36I DraI DraIII DrdII EagI Eco47III EcoRV FseI FspI HgiEII HindIII HpaI MfeI MluI MscI NarI NdeI NgoAIV NheI NotI PacI Pfl1108I PflMI PinAI PmeI PmlI PstI PvuI PvuII RcaI RsrII SacI SacII SanDI SapI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI Sse8387I Sse8647I SspI StuI SwaI VspI XbaI XcmI XmnI