(Linear) MAP of: ./Contig549.seq check: 6935 from: 1 to: 1444 Contig549 Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * March 20, 2001 16:47 .. VspI | ccccctccgagtttttttttttttttttttcaacagggacatcagagatgtattcaattagggACAAATTAATGTTCGTAAATAGCTTCGTATGGATCGC 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 gggggaggctcaaaaaaaaaaaaaaaaaaagttgtccctgtagtctctacataagttaatcccTGTTTAATTACAAGCATTTATCGAAGCATACCTAGCG a P P P S F F F F F F Q Q G H Q R C I Q L G T N * C S * I A S Y G S L - b P L R V F F F F F F N R D I R D V F N * G Q I N V R K * L R M D R - c P S E F F F F F F S T G T S E M Y S I R D K L M F V N S F V W I A - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d G E S N K K K K K E V P V D S I Y E I L S L N I N T F L K T H I A - e G R R T K K K K K K L L S M L S T N L * P C I L T R L Y S R I S R - f G G L K K K K K K * C P C * L H I * N P V F * H E Y I A E Y P D - ApaBI AlwNI| BspGI NsiI BbsI PvuII| | | | || TCCCAAGTTTTTCACGCCTGTGCTTGTACCCGTCCAGTTCCCGCTATGCATCATACATGATCCAACTTGGTCTTCCCTCTAAACAAATGCAGCAGCTGCC 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 AGGGTTCAAAAAGTGCGGACACGAACATGGGCAGGTCAAGGGCGATACGTAGTATGTACTAGGTTGAACCAGAAGGGAGATTTGTTTACGTCGTCGACGG a P S F S R L C L Y P S S S R Y A S Y M I Q L G L P S K Q M Q Q L P - b S Q V F H A C A C T R P V P A M H H T * S N L V F P L N K C S S C Q - c P K F F T P V L V P V Q F P L C I I H D P T W S S L * T N A A A A - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d G L N K V G T S T G T W N G S H M M C S G V Q D E R * V F A A A A - e E W T K * A Q A Q V R G T G A I C * V H D L K T K G R F L H L L Q - f S G L K E R R H K Y G D L E R * A D Y M I W S P R G E L C I C C S G - BglI NheI BamHI DrdII PvuII | | | | | AGAATGGCGGCACCAAGAGCCCCGATGCTAGCCCCGACCTTGGATCCGGCGTTACCAGAACCACCAGTCTCACTTGCATTGGCTCCATCAGCTGTAGATG 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 TCTTACCGCCGTGGTTCTCGGGGCTACGATCGGGGCTGGAACCTAGGCCGCAATGGTCTTGGTGGTCAGAGTGAACGTAACCGAGGTAGTCGACATCTAC a E W R H Q E P R C * P R P W I R R Y Q N H Q S H L H W L H Q L * M - b N G G T K S P D A S P D L G S G V T R T T S L T C I G S I S C R C - c R M A A P R A P M L A P T L D P A L P E P P V S L A L A P S A V D V - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d L I A A G L A G I S A G V K S G A N G S G G T E S A N A G D A T S - e W F P P V L L G S A L G S R P D P T V L V V L R V Q M P E M L Q L H - f S H R C W S G R H * G R G Q I R R * W F W W D * K C Q S W * S Y I - BsrDI | TTCCCGAGCCACTGCCGCTGCCGTTACCATTGCCACCCTTTCCTCCGCTGCCACTTCCACTTCCGCTTCCACCAGAACCTCCACTGCCACCAGATCCAGA 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 AAGGGCTCGGTGACGGCGACGGCAATGGTAACGGTGGGAAAGGAGGCGACGGTGAAGGTGAAGGCGAAGGTGGTCTTGGAGGTGACGGTGGTCTAGGTCT a F P S H C R C R Y H C H P F L R C H F H F R F H Q N L H C H Q I Q S - b S R A T A A A V T I A T L S S A A T S T S A S T R T S T A T R S R - c P E P L P L P L P L P P F P P L P L P L P L P P E P P L P P D P E - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d T G S G S G S G N G N G G K G G S G S G S G S G G S G G S G G S G S - e E R A V A A A T V M A V R E E A A V E V E A E V L V E V A V L D L - f N G L W Q R Q R * W Q W G K R R Q W K W K R K W W F R W Q W W I W - KpnI Psp1406I | | GCTTGACTTTATTCCGCTGTCGGTACCAGCACGAACGTTGCCAACGGTAAGAGGCGAAGGCTTGTCACCACACGACTGCGTGACTTGCACATTCGCAGCG 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 CGAACTGAAATAAGGCGACAGCCATGGTCGTGCTTGCAACGGTTGCCATTCTCCGCTTCCGAACAGTGGTGTGCTGACGCACTGAACGTGTAAGCGTCGC a L T L F R C R Y Q H E R C Q R * E A K A C H H T T A * L A H S Q R - b A * L Y S A V G T S T N V A N G K R R R L V T T R L R D L H I R S A - c L D F I P L S V P A R T L P T V R G E G L S P H D C V T C T F A A - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d S S K I G S D T G A R V N G V T L P S P K D G C S Q T V Q V N A A - e A Q S * E A T P V L V F T A L P L L R L S T V V R S R S K C M R L - f L K V K N R Q R Y W C S R Q W R Y S A F A Q * W V V A H S A C E C R - BbsI NgoAIV | | CCGGGGTTATCCGAAGACCGAATACCGTTGCTCTTGATGTCCTGGAACTTGCCATCGGTGGTGTATAGAGGTTCGCTGTAGACGTTTGCGCTGCCGGCTT 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 GGCCCCAATAGGCTTCTGGCTTATGGCAACGAGAACTACAGGACCTTGAACGGTAGCCACCACATATCTCCAAGCGACATCTGCAAACGCGACGGCCGAA a R G Y P K T E Y R C S * C P G T C H R W C I E V R C R R L R C R L - b G V I R R P N T V A L D V L E L A I G G V * R F A V D V C A A G L - c P G L S E D R I P L L L M S W N L P S V V Y R G S L * T F A L P A W - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d G P N D S S R I G N S K I D Q F K G D T T Y L P E S Y V N A S G A - e A P T I R L G F V T A R S T R S S A M P P T Y L N A T S T Q A A P K - f R P * G F V S Y R Q E Q H G P V Q W R H H I S T R Q L R K R Q R S - PstI BcgI Pfl1108I BcgI | | | | GGATCTGCAGGGTACAGTCCGTCTTTGTAGCTCGCTTTCTGAGTTTGGCACTTCCCTCGCCTACGAGGATGGATGCGACAATGTAGATGAGGTCGTCGTC 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 CCTAGACGTCCCATGTCAGGCAGAAACATCGAGCGAAAGACTCAAACCGTGAAGGGAGCGGATGCTCCTACCTACGCTGTTACATCTACTCCAGCAGCAG a G S A G Y S P S L * L A F * V W H F P R L R G W M R Q C R * G R R R - b D L Q G T V R L C S S L S E F G T S L A Y E D G C D N V D E V V V - c I C R V Q S V F V A R F L S L A L P S P T R M D A T M * M R S S S - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d Q I Q L T C D T K T A R K R L K A S G E G V L I S A V I Y I L D D D - e S R C P V T R R Q L E S E S N P V E R A * S S P H S L T S S T T T - f P D A P Y L G D K Y S A K Q T Q C K G R R R P H I R C H L H P R R - PinAI Eco57I | SalI| | Eco47III BclI || | | | GCTGAAGTTCTCGCTATCGGTGTCGACCGGTACGGTGAAGGTTGCCTTGCCGTTGGCGGGAGCGCTGAGTTTTATGGCGGGCTTTCCGTCAACTTTGATC 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 CGACTTCAAGAGCGATAGCCACAGCTGGCCATGCCACTTCCAACGGAACGGCAACCGCCCTCGCGACTCAAAATACCGCCCGAAAGGCAGTTGAAACTAG a * S S R Y R C R P V R * R L P C R W R E R * V L W R A F R Q L * S - b A E V L A I G V D R Y G E G C L A V G G S A E F Y G G L S V N F D Q - c L K F S L S V S T G T V K V A L P L A G A L S F M A G F P S T L I - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d S F N E S D T D V P V T F T A K G N A P A S L K I A P K G D V K I - e A S T R A I P T S R Y P S P Q R A T P P L A S N * P P S E T L K S - f R Q L E R * R H R G T R H L N G Q R Q R S R Q T K H R A K R * S Q D - NarI AceIII Bce83I| BcgI | || | AGCTCTGCGTTGTCGCCGATGGTGGCGTTCTTGAGATCGACTTCTACTACGGCGCCAGTGCTTGTTGGGGccgCGCtCTCGGTAGCAGTCATGGTGGCTG 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 TCGAGACGCAACAGCGGCTACCACCGCAAGAACTCTAGCTGAAGATGATGCCGCGGTCACGAACAACCCCggcGCGaGAGCCATCGTCAGTACCACCGAC a A L R C R R W W R S * D R L L L R R Q C L L G P R S R * Q S W W L - b L C V V A D G G V L E I D F Y Y G A S A C W G R A L G S S H G G C - c S S A L S P M V A F L R S T S T T A P V L V G A A L S V A V M V A V - 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 d L E A N D G I T A N K L D V E V V A G T S T P A A S E T A T M T A - e * S Q T T A S P P T R S I S K * * P A L A Q Q P R A R P L L * P P Q - f A R R Q R R H H R E Q S R S R S R R W H K N P G R E R Y C D H H S - AlwNI BcgI PvuII | BcgI |Bpu10I BcgI Bpu10I | | | | | | | | | TATCGGTCGCATCGTCATCGTCTGTTTCGGTGGCTGTAGCTGAGGCATCGTCGTCGGTAGCAGTAGCATCTGCGGTTGCTGTTCCCTCAGCTGCTGTTGT 901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1000 ATAGCCAGCGTAGCAGTAGCAGACAAAGCCACCGACATCGACTCCGTAGCAGCAGCCATCGTCATCGTAGACGCCAACGACAAGGGAGTCGACGACAACA a Y R S H R H R L F R W L * L R H R R R * Q * H L R L L F P Q L L L W - b I G R I V I V C F G G C S * G I V V G S S S I C G C C S L S C C C - c S V A S S S S V S V A V A E A S S S V A V A S A V A V P S A A V V - 901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1000 d T D T A D D D D T E T A T A S A D D D T A T A D A T A T G E A A T T - e I P R M T M T Q K P P Q L Q P M T T P L L L M Q P Q Q E R L Q Q Q - f Y R D C R * R R N R H S Y S L C R R R Y C Y C R R N S N G * S S N - GGCCTCGTCATCGTCGTCGCTATCGGTTGCTGTAGCTGTAGGCTGATCTCCGTCCGAGACTTGGGAAGCATCCCCTGTGGCTACGGGCTCGTCTGCTGTG 1001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1100 CCGGAGCAGTAGCAGCAGCGATAGCCAACGACATCGACATCCGACTAGAGGCAGGCTCTGAACCCTTCGTAGGGGACACCGATGCCCGAGCAGACGACAC a P R H R R R Y R L L * L * A D L R P R L G K H P L W L R A R L L W - b G L V I V V A I G C C S C R L I S V R D L G S I P C G Y G L V C C G - c A S S S S S L S V A V A V G * S P S E T W E A S P V A T G S S A V - 1001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1100 d A E D D D D S D T A T A T P Q D G D S V Q S A D G T A V P E D A T - e P R T M T T A I P Q Q L Q L S I E T R S K P L M G Q P * P S T Q Q - f H G R * R R R * R N S Y S Y A S R R G L S P F C G R H S R A R R S H - BcgI Bpu10I | PvuII BcgI PstI AlwNI| | | | | || | GGTTCTGCGACAGCTGTAGGCTCTGCATTTGTTTGTGGTGTGTCATCGTCGCCGCTATCTGATGGACTCGAAGGTTCTGCAGCAGGCGCTGAGGGTTGCG 1101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1200 CCAAGACGCTGTCGACATCCGAGACGTAAACAAACACCACACAGTAGCAGCGGCGATAGACTACCTGAGCTTCCAAGACGTCGTCCGCGACTCCCAACGC a V L R Q L * A L H L F V V C H R R R Y L M D S K V L Q Q A L R V A - b F C D S C R L C I C L W C V I V A A I * W T R R F C S R R * G L R - c G S A T A V G S A F V C G V S S S P L S D G L E G S A A G A E G C A - 1101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1200 d P E A V A T P E A N T Q P T D D D G S D S P S S P E A A P A S P Q - e P N Q S L Q L S Q M Q K H H T M T A A I Q H V R L N Q L L R Q P N R - f T R R C S Y A R C K N T T H * R R R * R I S E F T R C C A S L T A - Bpu10I Eco57I | | | CTGCGGGTTCTGAAGGTTGAGGGTCGGCGATGGCTGAGGGTTCGGGTTGTTGTTCTGGGGCTGGGTCTGCGACTGTTTGGGCGAGGCCGTTGGCAGCCAA 1201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1300 GACGCCCAAGACTTCCAACTCCCAGCCGCTACCGACTCCCAAGCCCAACAACAAGACCCCGACCCAGACGCTGACAAACCCGCTCCGGCAACCGTCGGTT a L R V L K V E G R R W L R V R V V V L G L G L R L F G R G R W Q P K - b C G F * R L R V G D G * G F G L L F W G W V C D C L G E A V G S Q - c A G S E G * G S A M A E G S G C C S G A G S A T V W A R P L A A K - 1201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1300 d A A P E S P Q P D A I A S P E P Q Q E P A P D A V T Q A L G N A A L - e Q P N Q L N L T P S P Q P N P N N N Q P Q T Q S Q K P S A T P L W - f S R T R F T S P R R H S L T R T T T R P S P R R S N P R P R Q C G - StuI BbsI | | AAGGCCTATGATGATGCTTGAAGCGAAGCGCATGTCGGCGAACGGTTGTCGGGTATATGTCGGTTTGAGTCTTCTGTTGATGCTATATTGCAAGCAAAAC 1301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1400 TTCCGGATACTACTACGAACTTCGCTTCGCGTACAGCCGCTTGCCAACAGCCCATATACAGCCAAACTCAGAAGACAACTACGATATAACGTTCGTTTTG a G L * * C L K R S A C R R T V V G Y M S V * V F C * C Y I A S K T - b K A Y D D A * S E A H V G E R L S G I C R F E S S V D A I L Q A K H - c R P M M M L E A K R M S A N G C R V Y V G L S L L L M L Y C K Q N - 1301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1400 d L G I I I S S A F R M D A F P Q R T Y T P K L R R N I S Y Q L C F - e F A * S S A Q L S A C T P S R N D P I H R N S D E T S A I N C A F - f F P R H H H K F R L A H R R V T T P Y I D T Q T K Q Q H * I A L L V - SspI BssSI EcoRI | | | ACGTAGAATATTCCGTGAAAATAGTaaagcctcgtgccgaattc 1401 ---------+---------+---------+---------+---- 1444 TGCATCTTATAAGGCACTTTTATCAtttcggagcacggcttaag a R R I F R E N S K A S C R I - b V E Y S V K I V K P R A E F - c T * N I P * K * * S L V P N - 1401 ---------+---------+---------+---------+---- 1444 d V Y F I G H F Y Y L R T G F E - e C T S Y E T F I T F G R A S N - f R L I N R S F L L A E H R I - Enzymes that do cut: AceIII AlwNI ApaBI BamHI BbsI Bce83I BcgI BclI BglI Bpu10I BspGI BsrDI BssSI DrdII Eco47III Eco57I EcoRI KpnI NarI NgoAIV NheI NsiI Pfl1108I PinAI Psp1406I PstI PvuII SalI SspI StuI VspI Enzymes that do not cut: AatII AflII AhdI ApaI ApaLI AscI AvrII BfiI BglII BplI BpmI Bpu1102I BsaI BsaBI BsaXI BsbI BseRI BsgI BsiWI BsmI BsmBI BspEI BspLU11I BspMI BsrBI BsrGI BssHII Bst1107I BstEII BstXI Bsu36I ClaI DraI DraIII DrdI EagI EarI EciI EcoNI EcoRV FseI FspI HgiEII HindIII HpaI MfeI MluI MscI NcoI NdeI NotI NruI NspV PacI PflMI PmeI PmlI PshAI PvuI RcaI RsrII SacI SacII SanDI SapI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI Sse8387I Sse8647I SwaI Tth111I XbaI XcmI XhoI XmnI