(Linear) MAP of: ./Contig699.seq check: 6481 from: 1 to: 1188 Contig699 Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * March 20, 2001 16:47 .. SalI EcoRI BssSI EarI | BspMI | | | | | GAATTCGGCACGAGGCTTCTGAATCATCAATCGCCTACCTATCTCTTCACTTGTCGACATACCAAACTAGCGAACTTTCCTCTTACACCTGCCTACCTAC 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 CTTAAGCCGTGCTCCGAAGACTTAGTAGTTAGCGGATGGATAGAGAAGTGAACAGCTGTATGGTTTGATCGCTTGAAAGGAGAATGTGGACGGATGGATG a E F G T R L L N H Q S P T Y L F T C R H T K L A N F P L T P A Y L P - b N S A R G F * I I N R L P I S S L V D I P N * R T F L L H L P T Y - c I R H E A S E S S I A Y L S L H L S T Y Q T S E L S S Y T C L P T - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d F E A R P K Q I M L R R G I E E S T S M G F * R V K R K C R G V * - e N P V L S R F * * D G V * R K V Q R C V L S A F K G R V G A * R - f I R C S A E S D D I A * R D R * K D V Y W V L S S E E * V Q R G V - EciI | CAGATCAACTTCGCAACCAGTTAAATACTGTCCACTGCCTATCCGCCCTCTCTGAGAATACGAATTGGCACTGTAACATCGCCTTTATCAACCCAAGTCC 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 GTCTAGTTGAAGCGTTGGTCAATTTATGACAGGTGACGGATAGGCGGGAGAGACTCTTATGCTTAACCGTGACATTGTAGCGGAAATAGTTGGGTTCAGG a D Q L R N Q L N T V H C L S A L S E N T N W H C N I A F I N P S P - b Q I N F A T S * I L S T A Y P P S L R I R I G T V T S P L S T Q V L - c R S T S Q P V K Y C P L P I R P L * E Y E L A L * H R L Y Q P K S - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d W I L K A V L * I S D V A * G G E R L I R I P V T V D G K D V W T - e G S * S R L W N F V T W Q R D A R E S F V F Q C Q L M A K I L G L G - f L D V E C G T L Y Q G S G I R G R Q S Y S N A S Y C R R * * G L D - AflII | TGCTTCAAGAAGTGTCTCGTTTTTCATCATCTCCCACTGCGAGGGCAAGCGACCTTTGACTGAACTTGAAGCAACAACATCTCGTCTTAAGCATCCCATA 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 ACGAAGTTCTTCACAGAGCAAAAAGTAGTAGAGGGTGACGCTCCCGTTCGCTGGAAACTGACTTGAACTTCGTTGTTGTAGAGCAGAATTCGTAGGGTAT a A S R S V S F F I I S H C E G K R P L T E L E A T T S R L K H P I - b L Q E V S R F S S S P T A R A S D L * L N L K Q Q H L V L S I P Y - c C F K K C L V F H H L P L R G Q A T F D * T * S N N I S S * A S H T - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d R S * S T D R K E D D G V A L A L S R Q S F K F C C C R T K L M G Y - e A E L L T E N K M M E W Q S P L R G K V S S S A V V D R R L C G M - f Q K L F H R T K * * R G S R P C A V K S Q V Q L L L M E D * A D W - BsaXI | CATATCCTCAATTACCGCACTCGCAAATCGCCTCGCGCTAACTACACCCTCTCCATCCTGCAAAATGACCCGATCTCACAAGGCCAACGACCGTGACCAC 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 GTATAGGAGTTAATGGCGTGAGCGTTTAGCGGAGCGCGATTGATGTGGGAGAGGTAGGACGTTTTACTGGGCTAGAGTGTTCCGGTTGCTGGCACTGGTG a H I L N Y R T R K S P R A N Y T L S I L Q N D P I S Q G Q R P * P Q - b I S S I T A L A N R L A L T T P S P S C K M T R S H K A N D R D H - c Y P Q L P H S Q I A S R * L H P L H P A K * P D L T R P T T V T T - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d M D E I V A S A F R R A S V V G E G D Q L I V R D * L A L S R S W - e C I R L * R V R L D G R A L * V R E M R C F S G I E C P W R G H G - f V Y G * N G C E C I A E R * S C G R W G A F H G S R V L G V V T V V - AatII BsrBI SalI| | || AAGGGCATCGCCGAGGGGACTATGCTCCCTACGGAAACTGTCCCTCGCTTCTTTGGTAAGAGCGGCTTTGCCGACGTCGACCCCAAGAAGGTGAAGAAGG 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 TTCCCGTAGCGGCTCCCCTGATACGAGGGATGCCTTTGACAGGGAGCGAAGAAACCATTCTCGCCGAAACGGCTGCAGCTGGGGTTCTTCCACTTCTTCC a G H R R G D Y A P Y G N C P S L L W * E R L C R R R P Q E G E E G - b K G I A E G T M L P T E T V P R F F G K S G F A D V D P K K V K K D - c R A S P R G L C S L R K L S L A S L V R A A L P T S T P R R * R R - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d L P M A S P V I S G V S V T G R K K P L L P K A S T S G L F T F F - e C P C R R P S * A G * P F Q G E S R Q Y S R S Q R R R G W S P S S P - f L A D G L P S H E R R F S D R A E K T L A A K G V D V G L L H L L - BfiI BsrBI | | ACGGCTCTGGTCGAGGCAACTGGGGAACTGTCAACGATGACATCGCTGGCGAGCAGTTCACCTTTACCAATACCCGCCGCCGCTCCAACAGTAGCAGCTT 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 TGCCGAGACCAGCTCCGTTGACCCCTTGACAGTTGCTACTGTAGCGACCGCTCGTCAAGTGGAAATGGTTATGGGCGGCGGCGAGGTTGTCATCGTCGAA a R L W S R Q L G N C Q R * H R W R A V H L Y Q Y P P P L Q Q * Q L - b G S G R G N W G T V N D D I A G E Q F T F T N T R R R S N S S S F - c T A L V E A T G E L S T M T S L A S S S P L P I P A A A P T V A A S - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d S P E P R P L Q P V T L S S M A P S C N V K V L V R R R E L L L L K - e R S Q D L C S P F Q * R H C R Q R A T * R * W Y G G G S W C Y C S - f V A R T S A V P S S D V I V D S A L L E G K G I G A A A G V T A A - EarI Eco57I BbsI BseRI| BbsI | | || | | CTCTCATGTTACCGATTTCAAGACTAAGTTTGAGGTCAATGAGCCCGAACCCGTCTTCGAGGAGTCACTTCACGGCCCTGAAGAGGAAGACCATGAAGAC 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 GAGAGTACAATGGCTAAAGTTCTGATTCAAACTCCAGTTACTCGGGCTTGGGCAGAAGCTCCTCAGTGAAGTGCCGGGACTTCTCCTTCTGGTACTTCTG a L S C Y R F Q D * V * G Q * A R T R L R G V T S R P * R G R P * R P - b S H V T D F K T K F E V N E P E P V F E E S L H G P E E E D H E D - c L M L P I S R L S L R S M S P N P S S R S H F T A L K R K T M K T - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d E * T V S K L V L N S T L S G S G T K S S D S * P G S S S S W S S - e R E H * R N * S * T Q P * H A R V R R R P T V E R G Q L P L G H L - f E R M N G I E L S L K L D I L G F G D E L L * K V A R F L F V M F V - BbsI EarI ClaI BsmI ApaBI | | | | | CTCACCAAGACCGACTCTTCCGAGAGTGGTCGATCTTCTTCTTGATCGATCTACTGTATCTTTACTGAGGGATCGGCATTCACTGCTGGTCGCTCTGTAT 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 GAGTGGTTCTGGCTGAGAAGGCTCTCACCAGCTAGAAGAAGAACTAGCTAGATGACATAGAAATGACTCCCTAGCCGTAAGTGACGACCAGCGAGACATA a H Q D R L F R E W S I F F L I D L L Y L Y * G I G I H C W S L C I - b L T K T D S S E S G R S S S * S I Y C I F T E G S A F T A G R S V * - c S P R P T L P R V V D L L L D R S T V S L L R D R H S L L V A L Y - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d R V L V S E E S L P R D E E Q D I * Q I K V S P D A N V A P R E T - e G * W S R S K R S H D I K K K I S R S Y R * Q P I P M * Q Q D S Q I - f E G L G V R G L T T S R R R S R D V T D K S L S R C E S S T A R Y - Pfl1108I | AGTGGCAGCGGCACATGAGGGGGATGGCATGTATTGAAATTGTGATGGACGTTTGATCTTTCATTTACAGCTACGAAACGAGATGCGATGGAACGAATGG 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 TCACCGTCGCCGTGTACTCCCCCTACCGTACATAACTTTAACACTACCTGCAAACTAGAAAGTAAATGTCGATGCTTTGCTCTACGCTACCTTGCTTACC a V A A A H E G D G M Y * N C D G R L I F H L Q L R N E M R W N E W - b W Q R H M R G M A C I E I V M D V * S F I Y S Y E T R C D G T N G - c S G S G T * G G W H V L K L * W T F D L S F T A T K R D A M E R M E - 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 d Y H C R C M L P I A H I S I T I S T Q D K M * L * S V L H S P V F P - e T A A A C S P S P M Y Q F Q S P R K I K * K C S R F S I R H F S H - f L P L P V H P P H C T N F N H H V N S R E N V A V F R S A I S R I - BglII BssSI | | AAACCAGAAATTATAACGCTTTTACAGCGGCAACGACGGATCAAACAATTTAGGGGCTATGGTAGCGGTTATGGATGGAGATGAGAAACTCAGATCTCCA 901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1000 TTTGGTCTTTAATATTGCGAAAATGTCGCCGTTGCTGCCTAGTTTGTTAAATCCCCGATACCATCGCCAATACCTACCTCTACTCTTTGAGTCTAGAGGT a K P E I I T L L Q R Q R R I K Q F R G Y G S G Y G W R * E T Q I S T - b N Q K L * R F Y S G N D G S N N L G A M V A V M D G D E K L R S P - c T R N Y N A F T A A T T D Q T I * G L W * R L W M E M R N S D L H - 901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1000 d F W F N Y R K * L P L S P D F L K P A I T A T I S P S S F S L D G - e F G S I I V S K C R C R R I L C N L P * P L P * P H L H S V * I E - f S V L F * L A K V A A V V S * V I * P S H Y R N H I S I L F E S R W - XcmI | CGAGGATCATGGCTTTTACGACCGGCTGCGAAGTTATCTTCCAAGCGGAACGATGTATTACTTGTATCACTATCTTAATTAGCTTTGACATCGAAGCGAG 1001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1100 GCTCCTAGTACCGAAAATGCTGGCCGACGCTTCAATAGAAGGTTCGCCTTGCTACATAATGAACATAGTGATAGAATTAATCGAAACTGTAGCTTCGCTC a R I M A F T T G C E V I F Q A E R C I T C I T I L I S F D I E A S - b R G S W L L R P A A K L S S K R N D V L L V S L S * L A L T S K R A - c E D H G F Y D R L R S Y L P S G T M Y Y L Y H Y L N * L * H R S E - 1001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1100 d R P D H S K R G A A F N D E L R F S T N S T D S D * N A K V D F R - e V L I M A K V V P Q S T I K W A S R H I V Q I V I K I L K S M S A L - f S S * P K * S R S R L * R G L P V I Y * K Y * * R L * S Q C R L S - PvuII PvuII XhoI ApaI KpnI | | | | | CAATACAGCTGGGCACAGCTGACAAACATCAgacaaagacaaaaaaaaaaaaaaaaaaaaactcgaggggggcccggtacccaaacgg 1101 ---------+---------+---------+---------+---------+---------+---------+---------+-------- 1188 GTTATGTCGACCCGTGTCGACTGTTTGTAGTctgtttctgtttttttttttttttttttttgagctccccccgggccatgggtttgcc a N T A G H S * Q T S D K D K K K K K K K L E G G P V P K R - b I Q L G T A D K H Q T K T K K K K K K N S R G A R Y P N - c Q Y S W A Q L T N I R Q R Q K K K K K K T R G G P G T Q T - 1101 ---------+---------+---------+---------+---------+---------+---------+---------+-------- 1188 d A I C S P V A S L C * V F V F F F F F F F E L P A R Y G F P - e L V A P C L Q C V D S L S L F F F F F F S S P P G T G L R - f C Y L Q A C S V F M L C L C F F F F F F V R P P G P V W V - Enzymes that do cut: AatII AflII ApaI ApaBI BbsI BfiI BglII BsaXI BseRI BsmI BspMI BsrBI BssSI ClaI EarI EciI Eco57I EcoRI KpnI Pfl1108I PvuII SalI XcmI XhoI Enzymes that do not cut: AceIII AhdI AlwNI ApaLI AscI AvrII BamHI Bce83I BcgI BclI BglI BplI BpmI Bpu10I Bpu1102I BsaI BsaBI BsbI BsgI BsiWI BsmBI BspEI BspGI BspLU11I BsrDI BsrGI BssHII Bst1107I BstEII BstXI Bsu36I DraI DraIII DrdI DrdII EagI Eco47III EcoNI EcoRV FseI FspI HgiEII HindIII HpaI MfeI MluI MscI NarI NcoI NdeI NgoAIV NheI NotI NruI NsiI NspV PacI PflMI PinAI PmeI PmlI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SacII SanDI SapI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI Sse8387I Sse8647I SspI StuI SwaI Tth111I VspI XbaI XmnI