(Linear) MAP of: ./Contig860.seq check: 5774 from: 1 to: 1009 Contig860 Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * March 20, 2001 16:47 .. EcoRI BssSI Bce83I AceIII EciI | | | | | GAATTCGGCACGAGGAACAATACCAGTACGCTCTCCAACAACAAGAACAACCTACACATCTACAACCATGTCAGCTCAAGACTACTACAGTGGCGGCGGA 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 CTTAAGCCGTGCTCCTTGTTATGGTCATGCGAGAGGTTGTTGTTCTTGTTGGATGTGTAGATGTTGGTACAGTCGAGTTCTGATGATGTCACCGCCGCCT a E F G T R N N T S T L S N N K N N L H I Y N H V S S R L L Q W R R R - b N S A R G T I P V R S P T T R T T Y T S T T M S A Q D Y Y S G G G - c I R H E E Q Y Q Y A L Q Q Q E Q P T H L Q P C Q L K T T T V A A E - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d I R C S S C Y W Y A R W C C S C G V C R C G H * S L V V V T A A S - e F E A R P V I G T R E G V V L V V * V D V V M D A * S * * L P P P - f N P V L F L V L V S E L L L F L R C M * L W T L E L S S C H R R - MscI Bpu10I | | GGCAGTGGTGGTGGCTACAACCAGCAGCAGCAAGGCTACGGACAGCAGCAAGGCTACGGACAACAAGAAGGCTATGGCCAACAGCAGGGCGGTTATCCTC 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 CCGTCACCACCACCGATGTTGGTCGTCGTCGTTCCGATGCCTGTCGTCGTTCCGATGCCTGTTGTTCTTCCGATACCGGTTGTCGTCCCGCCAATAGGAG a Q W W W L Q P A A A R L R T A A R L R T T R R L W P T A G R L S S - b G S G G G Y N Q Q Q Q G Y G Q Q Q G Y G Q Q E G Y G Q Q Q G G Y P Q - c A V V V A T T S S S K A T D S S K A T D N K K A M A N S R A V I L - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d A T T T A V V L L L L A V S L L L A V S L L F A I A L L L A T I R - e P L P P P * L W C C C P * P C C C P * P C C S P * P W C C P P * G - f L C H H H S C G A A A L S R V A A L S R V V L L S H G V A P R N D E - ScaI BsbI BglI | | | AGCAGCAACAGTACTCACAGGGCTCCAACTACGGTTCCCAACAGCCCAGCCACGATAACCGCGACCAGTCCTACGGTCAACACCAAGGCGGCCAATACGG 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 TCGTCGTTGTCATGAGTGTCCCGAGGTTGATGCCAAGGGTTGTCGGGTCGGTGCTATTGGCGCTGGTCAGGATGCCAGTTGTGGTTCCGCCGGTTATGCC a A A T V L T G L Q L R F P T A Q P R * P R P V L R S T P R R P I R - b Q Q Q Y S Q G S N Y G S Q Q P S H D N R D Q S Y G Q H Q G G Q Y G - c S S N S T H R A P T T V P N S P A T I T A T S P T V N T K A A N T A - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d L L L L V * L A G V V T G L L G A V I V A V L G V T L V L A A L V - e * C C C Y E C P E L * P E W C G L W S L R S W D * P * C W P P W Y P - f A A V T S V P S W S R N G V A W G R Y G R G T R R D V G L R G I R - BamHI BglI EciI| SfiI EciI BfiI || | | | CGGATCCAACGCCTACCCTGGTGGCCCTGGTGGCCCAGGTGGACCTGGCGGACCCGGAGGACCCGACGGCGAACGAGGACTGGGTGCTACACTTGCCGGA 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 GCCTAGGTTGCGGATGGGACCACCGGGACCACCGGGTCCACCTGGACCGCCTGGGCCTCCTGGGCTGCCGCTTGCTCCTGACCCACGATGTGAACGGCCT a R I Q R L P W W P W W P R W T W R T R R T R R R T R T G C Y T C R R - b G S N A Y P G G P G G P G G P G G P G G P D G E R G L G A T L A G - c D P T P T L V A L V A Q V D L A D P E D P T A N E D W V L H L P E - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d A S G V G V R T A R T A W T S R A S G S S G V A F S S Q T S C K G S - e P D L A * G P P G P P G P P G P P G P P G S P S R P S P A V S A P - f R I W R R G Q H G Q H G L H V Q R V R L V R R R V L V P H * V Q R - NgoAIV EagI | BglI| | BsaI Eco47III EagI || | | | | GGCGGTGCCGCTGGGTGGGCTGCCCACTCGGCCGGCAGTGGTCTCATAGGCAGTCTTGCCAGCGCTGCGGCCGGTGCCATTGGCGCAAACTTCATCGAGC 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 CCGCCACGGCGACCCACCCGACGGGTGAGCCGGCCGTCACCAGAGTATCCGTCAGAACGGTCGCGACGCCGGCCACGGTAACCGCGTTTGAAGTAGCTCG a R C R W V G C P L G R Q W S H R Q S C Q R C G R C H W R K L H R A - b G G A A G W A A H S A G S G L I G S L A S A A A G A I G A N F I E H - c A V P L G G L P T R P A V V S * A V L P A L R P V P L A Q T S S S - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d A T G S P P S G V R G A T T E Y A T K G A S R G T G N A C V E D L - e P P A A P H A A W E A P L P R M P L R A L A A A P A M P A F K M S - f L R H R Q T P Q G S P R C H D * L C D Q W R Q P R H W Q R L S * R A - BstXI XhoI SgrAI | | | ACAAGTTCAAGAAGCACAAGAAGGAGAAGAACAAGAAGCACCACAGAGATGGACGTGCTCGAGCTTACACCGGCGACTCGACCAGCAGCAGTGACTCCGA 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 TGTTCAAGTTCTTCGTGTTCTTCCTCTTCTTGTTCTTCGTGGTGTCTCTACCTGCACGAGCTCGAATGTGGCCGCTGAGCTGGTCGTCGTCACTGAGGCT a Q V Q E A Q E G E E Q E A P Q R W T C S S L H R R L D Q Q Q * L R - b K F K K H K K E K N K K H H R D G R A R A Y T G D S T S S S D S D - c T S S R S T R R R R T R S T T E M D V L E L T P A T R P A A V T P T - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d V L E L L V L L L L V L L V V S I S T S S S V G A V R G A A T V G - e C L N L F C L F S F F L F C W L S P R A R A * V P S E V L L L S E S - f C T * S A C S P S S C S A G C L H V H E L K C R R S S W C C H S R - NcoI XcmI | | | CTCTGGTGATGATCGACGCCGCAGTGACGACCGCCGACGACGTAGGGACGAGGATCTCGCCTACGGCAAGTCACAATATGACAACCAATCTAGCCATGGA 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 GAGACCACTACTAGCTGCGGCGTCACTGCTGGCGGCTGCTGCATCCCTGCTCCTAGAGCGGATGCCGTTCAGTGTTATACTGTTGGTTAGATCGGTACCT a L W * * S T P Q * R P P T T * G R G S R L R Q V T I * Q P I * P W R - b S G D D R R R S D D R R R R R D E D L A Y G K S Q Y D N Q S S H G - c L V M I D A A V T T A D D V G T R I S P T A S H N M T T N L A M E - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d V R T I I S A A T V V A S S T P V L I E G V A L * L I V V L R A M S - e E P S S R R R L S S R R R R L S S S R A * P L D C Y S L W D L W P - f S Q H H D V G C H R G G V V Y P R P D R R R C T V I H C G I * G H - NdeI | GGTTCTAGTCACTACGGTGGCTCACATGGCGGTTCATATGGTGGTCAGCAAGGCTATGGCAACCAAGGATACGGAGGACAGGGTGGATATGGAGGTAGAT 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 CCAAGATCAGTGATGCCACCGAGTGTACCGCCAAGTATACCACCAGTCGTTCCGATACCGTTGGTTCCTATGCCTCCTGTCCCACCTATACCTCCATCTA a F * S L R W L T W R F I W W S A R L W Q P R I R R T G W I W R * I - b G S S H Y G G S H G G S Y G G Q Q G Y G N Q G Y G G Q G G Y G G R Y - c V L V T T V A H M A V H M V V S K A M A T K D T E D R V D M E V D - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d T R T V V T A * M A T * I T T L L A I A V L S V S S L T S I S T S - e P E L * * P P E C P P E Y P P * C P * P L W P Y P P C P P Y P P L - f L N * D S R H S V H R N M H H D A L S H C G L I R L V P H I H L Y I - DraI | ACTAATTCCTTTGCATTTAAAAGTACAAAATGCGAGAGGATATAATGAGGCATGAATGAATAGATTAGTCATTAGGCACGTTGGAACAGACATGAGAATA 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 TGATTAAGGAAACGTAAATTTTCATGTTTTACGCTCTCCTATATTACTCCGTACTTACTTATCTAATCAGTAATCCGTGCAACCTTGTCTGTACTCTTAT a L I P L H L K V Q N A R G Y N E A * M N R L V I R H V G T D M R I - b * F L C I * K Y K M R E D I M R H E * I D * S L G T L E Q T * E Y - c T N S F A F K S T K C E R I * * G M N E * I S H * A R W N R H E N T - 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 d V L E K A N L L V F H S L I Y H P M F S Y I L * * A R Q F L C S F - e Y * N R Q M * F Y L I R S S I I L C S H I S * D N P V N S C V H S Y - f S I G K C K F T C F A L P Y L S A H I F L N T M L C T P V S M L I - ApaI | CGAACAATAAGAACAATGCTTCACTAAAAAAAAAAAAAAAAAAAAAAaaaaaaaacaaaaaaaaaaaaaaaaaaaaaaaactcgaaggggggggcccggt 901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1000 GCTTGTTATTCTTGTTACGAAGTGATTTTTTTTTTTTTTTTTTTTTTttttttttgttttttttttttttttttttttttgagcttccccccccgggcca a R T I R T M L H * K K K K K K K K K T K K K K K K K N S K G G A R Y - b E Q * E Q C F T K K K K K K K K K K Q K K K K K K K T R R G G P G - c N N K N N A S L K K K K K K K K K N K K K K K K K K L E G G G P V - 901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1000 d V F L L F L A E S F F F F F F F F F F L F F F F F F F S S P P P G T - e S C Y S C H K V L F F F F F F F F F C F F F F F F F V R L P P G P - f R V I L V I S * * F F F F F F F F F V F F F F F F F F E F P P A R - KpnI | acccaaggt 1001 --------- 1009 tgggttcca a P R - b T Q G - c P K - 1001 --------- 1009 d G L T - e V W P - f Y G L - Enzymes that do cut: AceIII ApaI BamHI Bce83I BfiI BglI Bpu10I BsaI BsbI BssSI BstXI DraI EagI EciI Eco47III EcoRI KpnI MscI NcoI NdeI NgoAIV ScaI SfiI SgrAI XcmI XhoI Enzymes that do not cut: AatII AflII AhdI AlwNI ApaBI ApaLI AscI AvrII BbsI BcgI BclI BglII BplI BpmI Bpu1102I BsaBI BsaXI BseRI BsgI BsiWI BsmI BsmBI BspEI BspGI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII Bst1107I BstEII Bsu36I ClaI DraIII DrdI DrdII EarI Eco57I EcoNI EcoRV FseI FspI HgiEII HindIII HpaI MfeI MluI NarI NheI NotI NruI NsiI NspV PacI Pfl1108I PflMI PinAI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SanDI SapI SexAI SgfI SmaI SnaBI SpeI SphI SrfI Sse8387I Sse8647I SspI StuI SwaI Tth111I VspI XbaI XmnI