BLASTX 2.1.3 [Apr-1-2001]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query=
/home/local/jxu/web/Fgr/NCPvsCOGs/blast/0941_A06_A11ZS5.seq
(963 letters)
Database: COGs
78,847 sequences; 26,228,252 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ML1556 ML1556 COG0532 [J] Translation initiation factor 2 (GTPa... 33 0.72
VNG1142G VNG1142G COG0148 [G] Enolase 30 6.1
RV2839C Rv2839c COG0532 [J] Translation initiation factor 2 (GT... 30 8.0
>ML1556 ML1556 COG0532 [J] Translation initiation factor 2 (GTPase)
Length = 924
Score = 33.1 bits (74), Expect = 0.72
Identities = 24/70 (34%), Positives = 26/70 (36%), Gaps = 14/70 (20%)
Frame = +1
Query: 694 GAGGCGCPVVLLLEVGG---------AWEGRGXWWVAGQRGGGKHAGDA-----GAVCGX 831
G G G PV + GG A G G G+ GGG H G G G
Sbjct: 231 GGGRPGAPVGGRSDAGGGNYRGGGVGALPGGGSGGFRGRPGGGGHGGGGRPGQRGGAAGA 290
Query: 832 LSRPMGGPRR 861
RP G PRR
Sbjct: 291 FGRPGGAPRR 300
>VNG1142G VNG1142G COG0148 [G] Enolase
Length = 398
Score = 30.0 bits (66), Expect = 6.1
Identities = 29/83 (34%), Positives = 35/83 (41%), Gaps = 1/83 (1%)
Frame = +1
Query: 610 ADDSSDLWRPVAVSAHAVVPCRRRASGWGAGGCGCPVVLLLEVGGAWEGRGXWWVAGQ-R 786
ADD++D AV A+ V A+ A G V +GGA GR G
Sbjct: 87 ADDTTDF---SAVGANVAVATSMAAAKAAADVLG--VHTYQHLGGALRGRNFPVPLGNVL 141
Query: 787 GGGKHAGDAGAVCGXLSRPMGGP 855
GGG HA DA A+ L G P
Sbjct: 142 GGGAHAADATAIQEFLVAATGAP 164
>RV2839C Rv2839c COG0532 [J] Translation initiation factor 2
(GTPase)
Length = 900
Score = 29.6 bits (65), Expect = 8.0
Identities = 17/40 (42%), Positives = 18/40 (44%)
Frame = +1
Query: 742 GAWEGRGXWWVAGQRGGGKHAGDAGAVCGXLSRPMGGPRR 861
GA G G G GGG+ G G G RP G PRR
Sbjct: 238 GAAPGTGFRGRPGGGGGGR-PGQRGGAAGAFGRPGGAPRR 276
Database: COGs
Posted date: Jan 30, 2002 4:01 PM
Number of letters in database: 26,228,252
Number of sequences in database: 78,847
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 27788303
Number of Sequences: 78847
Number of extensions: 381266
Number of successful extensions: 1167
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 1136
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1164
length of query: 321
length of database: 26,228,252
effective HSP length: 49
effective length of query: 271
effective length of database: 22,364,749
effective search space: 6060846979
effective search space used: 6060846979
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 64 (29.3 bits)