BLASTX 2.1.3 [Apr-1-2001]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query=
/home/local/jxu/web/Fgr/NCPvsCOGs/blast/0941_C08_E15ZS5.seq
         (897 letters)

Database: COGs
           78,847 sequences; 26,228,252 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

L56488 L56488  COG0436 [E] PLP-dependent aminotransferases             33  0.51
MTH1109 MTH1109  COG1641 [S] Uncharacterized ACR                       32  1.9
CJ0625 Cj0625  COG0409 [O] Hydrogenase maturation factor               30  4.3

>L56488 L56488  COG0436 [E] PLP-dependent aminotransferases
          Length = 391

 Score = 33.5 bits (75), Expect = 0.51
 Identities = 20/77 (25%), Positives = 35/77 (44%)
 Frame = +2

Query: 371 FNLRAHRFEIAVIEIYDQSRADNKDVLKLILGKHNLSAPMTSYSRPEVMVKSQSYFFTHS 550
           FN    + EI++I  +DQ  +   DV+KL LG+ +   P        V +++    +T  
Sbjct: 7   FNPNLDKIEISLIRQFDQQVSSIPDVIKLTLGEPDFYTPEHVKQAGIVAIENNQSHYTGM 66

Query: 551 VNAMAVTQTAKGFTFKQ 601
              + + Q A  F  K+
Sbjct: 67  AGLLELRQAASEFMNKK 83



>MTH1109 MTH1109  COG1641 [S] Uncharacterized ACR
          Length = 418

 Score = 31.6 bits (70), Expect = 1.9
 Identities = 29/111 (26%), Positives = 45/111 (40%)
 Frame = +2

Query: 83  ISVSEARKMNEVVHTQAKIIADQDVMYKYLSKNLIFVATVSPKAAGEIGSAAPEEASLVA 262
           +SVSE ++   +  T   + AD+ +   YL    +      P    E+ S A      +A
Sbjct: 54  VSVSEVKRAG-LRATYVDVRADESLSVGYLEFLRLLEGISHPALDDEMLSMARAVFHTIA 112

Query: 263 YLIDAVSGRILHRVTHHGAQGPVHAVVSENWVVYHYFNLRAHRFEIAVIEI 415
               +V G  L  V  H   G   AV      V+ YF+L  HR E+  + +
Sbjct: 113 QAEASVHGVKLDEVHFHEV-GAADAVADVMGAVFAYFDLNLHRDEVYTLPV 162



>CJ0625 Cj0625  COG0409 [O] Hydrogenase maturation factor
          Length = 363

 Score = 30.4 bits (67), Expect = 4.3
 Identities = 28/130 (21%), Positives = 51/130 (38%)
 Frame = +2

Query: 53  GPSFSLPSRKISVSEARKMNEVVHTQAKIIADQDVMYKYLSKNLIFVATVSPKAAGEIGS 232
           G    +P  +IS+ + R     V      +   ++  + L+KN+IF A           +
Sbjct: 90  GDLLRVPGSEISLLDLRAKGADVRALYSPLEVLEIAKQNLNKNIIFFAI-------GFET 142

Query: 233 AAPEEASLVAYLIDAVSGRILHRVTHHGAQGPVHAVVSENWVVYHYFNLRAHRFEIAVIE 412
             P  A L+  +I+     +   + H     PV A++++  V  + F   +H   I    
Sbjct: 143 TTPMSALLLQKVIEEKINNVFFHINHITVPAPVEAIMNDENVKINAFLGPSHVSVITGYG 202

Query: 413 IYDQSRADNK 442
           IY+   A  K
Sbjct: 203 IYEPLAAKFK 212



  Database: COGs
    Posted date:  Jan 30, 2002  4:01 PM
  Number of letters in database: 26,228,252
  Number of sequences in database:  78,847
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39612597
Number of Sequences: 78847
Number of extensions: 792570
Number of successful extensions: 1916
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 1877
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1916
length of query: 299
length of database: 26,228,252
effective HSP length: 49
effective length of query: 249
effective length of database: 22,364,749
effective search space: 5568822501
effective search space used: 5568822501
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 64 (29.3 bits)