BLASTX 2.1.3 [Apr-1-2001]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query=
/home/local/jxu/web/Fgr/NCPvsCOGs/blast/0941_E04_I07ZS5.seq
(909 letters)
Database: COGs
78,847 sequences; 26,228,252 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BS_GLTX BS_gltX COG0008 [J] Glutamyl- and glutaminyl-tRNA synth... 30 5.7
BS_YQXC BS_yqxC COG1189 [J] Predicted rRNA methylases 30 5.7
MLR3498 mlr3498 COG1130 [G] ABC-type sugar/spermidine/putrescin... 30 7.5
>BS_GLTX BS_gltX COG0008 [J] Glutamyl- and glutaminyl-tRNA
synthetases
Length = 483
Score = 30.0 bits (66), Expect = 5.7
Identities = 25/71 (35%), Positives = 35/71 (49%), Gaps = 6/71 (8%)
Frame = +3
Query: 711 SGHLYAGPYSXALHCSFNYGF---K*SKFCL--DSSKKKKNFKGGPGSHLS-LIWSGFTI 872
+GHL+ G AL FNY F + KF + + + KK+N +GG S L+ L W G
Sbjct: 14 TGHLHIGNARTAL---FNYLFARNQGGKFIIRVEDTDKKRNIEGGEQSQLNYLKWLGIDW 70
Query: 873 QRXVILTPVYG 905
V + YG
Sbjct: 71 DESVDVGGEYG 81
>BS_YQXC BS_yqxC COG1189 [J] Predicted rRNA methylases
Length = 269
Score = 30.0 bits (66), Expect = 5.7
Identities = 15/39 (38%), Positives = 24/39 (61%)
Frame = +3
Query: 222 ENQKEVIVDQTGEREATYEDFTKTLPENDCRFAVFDFDF 338
++++ V++++T R AT DFTK +PE FA D F
Sbjct: 124 QDERVVVMERTNFRYATPADFTKGMPE----FATIDVSF 158
>MLR3498 mlr3498 COG1130 [G] ABC-type
sugar/spermidine/putrescine/iron/thiamine transpor
Length = 360
Score = 29.6 bits (65), Expect = 7.5
Identities = 26/96 (27%), Positives = 37/96 (38%), Gaps = 8/96 (8%)
Frame = -2
Query: 500 FARVSGLHLNLDAVQG----PLELLVGARVRHLAPHLCG----VRRPEDIEDPALWHILR 345
F + GLH + V L++ G+R + P CG +R E P I+
Sbjct: 3 FLEIKGLHKHYGPVAALAGVDLDVASGSRTAIVGPSGCGKTTLLRLIAGFEAPDQGRIVL 62
Query: 344 GGEVEVKHCESAVVLRQGLGEVLIGGLALPSLVHND 237
GEV + R+G+G V G P L D
Sbjct: 63 DGEVLANGGAAVPAHRRGIGVVAQDGALFPHLTIGD 98
Database: COGs
Posted date: Jan 30, 2002 4:01 PM
Number of letters in database: 26,228,252
Number of sequences in database: 78,847
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39429767
Number of Sequences: 78847
Number of extensions: 789986
Number of successful extensions: 2071
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 2029
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2071
length of query: 303
length of database: 26,228,252
effective HSP length: 49
effective length of query: 253
effective length of database: 22,364,749
effective search space: 5658281497
effective search space used: 5658281497
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 64 (29.3 bits)