BLASTX 2.1.3 [Apr-1-2001]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query=
/home/local/jxu/web/Fgr/NCPvsCOGs/blast/0941_F12_K23ZS5.seq
(923 letters)
Database: COGs
78,847 sequences; 26,228,252 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
NMB0406 NMB0406 COG3087 [D] Cell division protein 31 2.6
YLR197W YLR197w COG1498 [J] Protein implicated in ribosomal bio... 31 3.4
YPL240C YPL240c COG0326 [O] Molecular chaperone, HSP90 family 30 4.5
AQ_203 aq_203 COG0224 [C] F0F1-type ATP synthase gamma subunit 30 5.8
AQ_1677 aq_1677 COG0173 [J] Aspartyl-tRNA synthetase 30 7.6
YLL019C YLL019c COG0515 [T] Serine/threonine protein kinases 30 7.6
>NMB0406 NMB0406 COG3087 [D] Cell division protein
Length = 289
Score = 31.2 bits (69), Expect = 2.6
Identities = 19/43 (44%), Positives = 25/43 (57%)
Frame = -3
Query: 747 INQK*EEVKEKYTTGKRKREENEGQGKRPKGKEIEEEQHRREK 619
+ K +EV+EK G+ +REE +GQ R K E EQ REK
Sbjct: 100 VADKADEVEEK--AGEPEREEPDGQAVRKKALTEEREQTVREK 140
>YLR197W YLR197w COG1498 [J] Protein implicated in ribosomal
biogenesis, Nop56p homolog
Length = 504
Score = 30.8 bits (68), Expect = 3.4
Identities = 14/43 (32%), Positives = 27/43 (62%)
Frame = -3
Query: 732 EEVKEKYTTGKRKREENEGQGKRPKGKEIEEEQHRREKTGSNK 604
EE KEK ++ KRK E+++ + K K K+ ++E+ +++ K
Sbjct: 445 EETKEKESSKKRKLEDDDEEKKEKKEKKSKKEKKEKKEKKDKK 487
>YPL240C YPL240c COG0326 [O] Molecular chaperone, HSP90 family
Length = 709
Score = 30.4 bits (67), Expect = 4.5
Identities = 12/36 (33%), Positives = 23/36 (63%)
Frame = -3
Query: 723 KEKYTTGKRKREENEGQGKRPKGKEIEEEQHRREKT 616
+EK K+ E+ + K+PK +E++EE+ ++ KT
Sbjct: 222 EEKKDEEKKDEEKKDEDDKKPKLEEVDEEEEKKPKT 257
>AQ_203 aq_203 COG0224 [C] F0F1-type ATP synthase gamma subunit
Length = 393
Score = 30.0 bits (66), Expect = 5.8
Identities = 13/23 (56%), Positives = 17/23 (73%)
Frame = -3
Query: 690 EENEGQGKRPKGKEIEEEQHRRE 622
E EG+GKR GK I+EE+ +RE
Sbjct: 165 ERAEGKGKRETGKSIKEEEVKRE 187
>AQ_1677 aq_1677 COG0173 [J] Aspartyl-tRNA synthetase
Length = 603
Score = 29.6 bits (65), Expect = 7.6
Identities = 14/25 (56%), Positives = 17/25 (68%)
Frame = +2
Query: 362 ATGKDRVVALPSALSGMRNTLGFPK 436
A G DRVVAL L +R+T+ FPK
Sbjct: 550 AFGLDRVVALMLGLDSIRDTIAFPK 574
>YLL019C YLL019c COG0515 [T] Serine/threonine protein kinases
Length = 737
Score = 29.6 bits (65), Expect = 7.6
Identities = 14/41 (34%), Positives = 19/41 (46%)
Frame = +3
Query: 366 PGRIGWWHYQAPFLG*GIHWGFPRECSFWEAYCAGCS*ILG 488
P I HY+AP + G+ W FP C W C ++G
Sbjct: 502 PPVISTRHYRAPEIVLGLGWSFP--CDIWSIACVLVELVIG 540
Database: COGs
Posted date: Jan 30, 2002 4:01 PM
Number of letters in database: 26,228,252
Number of sequences in database: 78,847
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37566362
Number of Sequences: 78847
Number of extensions: 759923
Number of successful extensions: 2190
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 2089
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2183
length of query: 307
length of database: 26,228,252
effective HSP length: 49
effective length of query: 258
effective length of database: 22,364,749
effective search space: 5770105242
effective search space used: 5770105242
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 64 (29.3 bits)