BLASTX 2.1.3 [Apr-1-2001]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query=
/home/local/jxu/web/Fgr/NCPvsCOGs/blast/0941_G01_M01ZS5.seq
(797 letters)
Database: COGs
78,847 sequences; 26,228,252 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
YECS yecS COG0765 [E] ABC-type amino acid transport system, per... 31 2.2
ZYECS ZyecS COG0765 [E] ABC-type amino acid transport system, p... 31 2.2
CDA6787 CDa6787 COG0657 [I] Esterase/lipase 31 2.9
APE1140 APE1140 COG0527 [E] Aspartokinases 30 4.9
>YECS yecS COG0765 [E] ABC-type amino acid transport system,
permease component
Length = 222
Score = 31.2 bits (69), Expect = 2.2
Identities = 17/47 (36%), Positives = 21/47 (44%)
Frame = +1
Query: 322 PWNNQRRATFPPIRRIEGPPLSREFLKKS*TPKILALGDKKKTPHLF 462
PW RRA P R+ PPLS F+ K +L + P LF
Sbjct: 129 PWQTMRRAILPQAARVALPPLSNSFIS---LVKDTSLAATIQVPELF 172
>ZYECS ZyecS COG0765 [E] ABC-type amino acid transport system,
permease component
Length = 222
Score = 31.2 bits (69), Expect = 2.2
Identities = 17/47 (36%), Positives = 21/47 (44%)
Frame = +1
Query: 322 PWNNQRRATFPPIRRIEGPPLSREFLKKS*TPKILALGDKKKTPHLF 462
PW RRA P R+ PPLS F+ K +L + P LF
Sbjct: 129 PWQTMRRAILPQAARVALPPLSNSFIS---LVKDTSLAATIQVPELF 172
>CDA6787 CDa6787 COG0657 [I] Esterase/lipase
Length = 416
Score = 30.8 bits (68), Expect = 2.9
Identities = 22/78 (28%), Positives = 35/78 (44%), Gaps = 13/78 (16%)
Frame = -1
Query: 470 PQKKRWGVFFLSPKAKIFGVQ---------DFFKNSLERGGPSILRMG----GKVALLWL 330
PQK + + L K G + D + N ++ G +IL MG G +AL++L
Sbjct: 141 PQKSKLSILVLDYKLACHGYKVPYQLTELIDTYTNLVKDGSKNILLMGDSAGGNLALIFL 200
Query: 329 FQGRESKLDMPAPGETML 276
+ KL +P P +L
Sbjct: 201 QSLKIDKLSLPYPSSVLL 218
>APE1140 APE1140 COG0527 [E] Aspartokinases
Length = 473
Score = 30.0 bits (66), Expect = 4.9
Identities = 29/99 (29%), Positives = 42/99 (42%), Gaps = 8/99 (8%)
Frame = -1
Query: 416 GVQDFFKNSLERGGPSILRMGGKVALLWLFQGRESKL--------DMPAPGETMLNWGSK 261
GV D +S ER P + GG V +L RE L + + + L W ++
Sbjct: 69 GVTDALLSSFERRDPGV---GGAVGKTYLSIARELGLPPESVRDVSLASAEVSKLLWAAE 125
Query: 260 TSIPWTTR*RLGIVNGHVRLGTALRCXLVFRQFFLERYW 144
WT R R +V+ RL AL L+ R+ +E W
Sbjct: 126 VLGQWTPRVRDLVVSYGERLSAALMAGLL-RRLGVEARW 163
Database: COGs
Posted date: Jan 30, 2002 4:01 PM
Number of letters in database: 26,228,252
Number of sequences in database: 78,847
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 33485875
Number of Sequences: 78847
Number of extensions: 681343
Number of successful extensions: 1482
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 1445
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1482
length of query: 265
length of database: 26,228,252
effective HSP length: 49
effective length of query: 216
effective length of database: 22,364,749
effective search space: 4830785784
effective search space used: 4830785784
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 63 (28.9 bits)