BLASTX 2.1.3 [Apr-1-2001]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query=
/home/local/jxu/web/Fgr/NCPvsCOGs/blast/0941_H11_O21ZS5.seq
(900 letters)
Database: COGs
78,847 sequences; 26,228,252 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BU166 BU166 COG1007 [C] NADH:ubiquinone oxidoreductase subunit ... 31 2.5
TP0714 TP0714 COG1298 [N] Flagellar biosynthesis/type III secre... 29 9.7
>BU166 BU166 COG1007 [C] NADH:ubiquinone oxidoreductase subunit 2
(chain N)
Length = 469
Score = 31.2 bits (69), Expect = 2.5
Identities = 24/77 (31%), Positives = 38/77 (49%), Gaps = 3/77 (3%)
Frame = -2
Query: 755 PVH-WTKDIYRHCFDNVCSGHSTLGKASFYPIM--FKKPDSPDEANKAFLIISSNKHIXR 585
P H WT DIY+ +V S ST GK S + ++ F S + + I+S +
Sbjct: 206 PFHLWTPDIYQGSPTSVLSFFSTAGKISVFSVLLNFLSYFSNSDNKVIYFILSLIIILSI 265
Query: 584 LLLPYVASFLLDLERFI 534
L+ +A F D++RF+
Sbjct: 266 LVGNLMALFQKDIKRFL 282
>TP0714 TP0714 COG1298 [N] Flagellar biosynthesis/type III
secretory pathway protein
Length = 707
Score = 29.3 bits (64), Expect = 9.7
Identities = 10/21 (47%), Positives = 15/21 (70%)
Frame = +1
Query: 205 GYLPSLMSRNALRSMMWGCFF 267
GY+P L++ + +RS MW FF
Sbjct: 647 GYIPVLLTTDTIRSAMWNLFF 667
Database: COGs
Posted date: Jan 30, 2002 4:01 PM
Number of letters in database: 26,228,252
Number of sequences in database: 78,847
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 36973622
Number of Sequences: 78847
Number of extensions: 707787
Number of successful extensions: 1770
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 1749
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1770
length of query: 300
length of database: 26,228,252
effective HSP length: 49
effective length of query: 250
effective length of database: 22,364,749
effective search space: 5591187250
effective search space used: 5591187250
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 64 (29.3 bits)