BLASTX 2.1.3 [Apr-1-2001]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query=
/home/local/jxu/web/Fgr/NCPvsCOGs/blast/0941_H12_O23ZS5.seq
(928 letters)
Database: COGs
78,847 sequences; 26,228,252 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
MLL2069 mll2069 COG2319 [R] WD40 repeat protein 35 0.24
BS_YVRK BS_yvrK COG2140 [G] Thermophilic glucose-6-phosphate is... 34 0.41
PM1080 PM1080 COG1120 [P] ABC-type cobalamin/Fe3+-siderophores ... 32 1.5
MJ0791 MJ0791 COG0165 [E] Argininosuccinate lyase 30 4.5
>MLL2069 mll2069 COG2319 [R] WD40 repeat protein
Length = 586
Score = 34.7 bits (78), Expect = 0.24
Identities = 20/50 (40%), Positives = 29/50 (58%), Gaps = 1/50 (2%)
Frame = +2
Query: 206 CDHNLSVNIDDPSRADI-YNPRAGTITRLNSQTFPILNIVQMSATRVHLY 352
CDHNL ++ PS A + + PR T+TRL + +L + +A VHLY
Sbjct: 36 CDHNLPISTPMPSPAGLPHEPR--TMTRLADRLLAVLLMALAAACLVHLY 83
>BS_YVRK BS_yvrK COG2140 [G] Thermophilic glucose-6-phosphate
isomerase and related meta
Length = 385
Score = 33.9 bits (76), Expect = 0.41
Identities = 27/117 (23%), Positives = 52/117 (44%)
Frame = +2
Query: 326 MSATRVHLYQNAIISPLWNINAHSVMYMIQGHIWVQVVNDHGRNVFNDLLSPGQLLIIPQ 505
+++ + L AI W+ A YMI G V +V++ GR+ +D + G L P
Sbjct: 79 LASVNMRLKPGAIRELHWHKEAEWA-YMIYGSARVTIVDEKGRSFIDD-VGEGDLWYFPS 136
Query: 506 NYVVLKKAQRDGSKYIEFKTNANSMVSHIAGKNSILGALPVDVIANAYGISRTEARN 676
+A +G++++ + + + L P +VIA +G+++ E N
Sbjct: 137 GLPHSIQALEEGAEFLLVFDDGSFSENSTFQLTDWLAHTPKEVIAANFGVTKEEISN 193
>PM1080 PM1080 COG1120 [P] ABC-type cobalamin/Fe3+-siderophores
transport systems, ATPa
Length = 245
Score = 32.0 bits (71), Expect = 1.5
Identities = 14/49 (28%), Positives = 24/49 (48%)
Frame = -3
Query: 239 DRRCSHLDYDHKSSPLNHSNHHKLIVMHEIALHEVDIPDMALPEIVLLE 93
D C+HLD H+ S + HK + LH++D+ I+L++
Sbjct: 157 DEPCNHLDIRHQQSLMQFLKQHKTQFNAVMVLHDLDLAASYADHIILMQ 205
>MJ0791 MJ0791 COG0165 [E] Argininosuccinate lyase
Length = 484
Score = 30.4 bits (67), Expect = 4.5
Identities = 20/69 (28%), Positives = 37/69 (52%), Gaps = 5/69 (7%)
Frame = -1
Query: 592 DVTNHGVRISLEFNVLASITLCLLQNNIVLWYD**LPR--AKKVIEHISTMV---IDNLN 428
DV + +S + + + LC + + I+L+ + + AKK+IE + + ++NLN
Sbjct: 16 DVAKYTTSLSFDKEIFEADILCDIAHVIMLYEQGIIKKEDAKKIIEGLKEIYKKGMENLN 75
Query: 427 PDMSLDHVH 401
D SLD +H
Sbjct: 76 LDPSLDDIH 84
Database: COGs
Posted date: Jan 30, 2002 4:01 PM
Number of letters in database: 26,228,252
Number of sequences in database: 78,847
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39552097
Number of Sequences: 78847
Number of extensions: 798279
Number of successful extensions: 2154
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 2102
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2153
length of query: 309
length of database: 26,228,252
effective HSP length: 49
effective length of query: 259
effective length of database: 22,364,749
effective search space: 5792469991
effective search space used: 5792469991
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 64 (29.3 bits)