BLASTX 2.1.3 [Apr-1-2001]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query=
/home/local/jxu/web/Fgr/NCPvsCOGs/blast/1605_B06_C11ZS5.seq
(1332 letters)
Database: COGs
78,847 sequences; 26,228,252 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CDA8833 CDa8833 COG0457 [R] TPR-repeat-containing proteins 31 0.11
TP0001 TP0001 COG0593 [L] ATPase involved in DNA replication in... 33 1.4
CDA9099 CDa9099 COG1112 [L] Superfamily I DNA and RNA helicases... 31 5.2
BH0276 BH0276 COG0577 [R] ABC-type transport systems, involved ... 31 5.2
CDA8969 CDa8969 COG0470 [L] ATPase involved in DNA replication 30 8.9
SLR0744 slr0744 COG0532 [J] Translation initiation factor 2 (GT... 30 8.9
>CDA8833 CDa8833 COG0457 [R] TPR-repeat-containing proteins
Length = 1080
Score = 31.2 bits (69), Expect(2) = 0.11
Identities = 21/97 (21%), Positives = 36/97 (36%), Gaps = 1/97 (1%)
Frame = -1
Query: 579 PHTKNN-EERTKIETQGATHVTDRHAVALLFAVYSNTPHRPPQHGRTSPGPTPPARPILR 403
PH K E+ TK + +G TH + + Q + P PP + +
Sbjct: 466 PHIKARLEQLTKYQQEGNTHPPQPPPSSQQPRLPQGMVLESTQQQQQQQPPPPPQQQQQQ 525
Query: 402 RNHKARAQTPPTQQNHITQEQKTTQHNTQQPTTEAHP 292
H++++Q P Q + QH + P + P
Sbjct: 526 LQHQSQSQPQPQQPPQTQSQPSLLQHQSSLPPQQIQP 562
Score = 23.9 bits (50), Expect(2) = 0.11
Identities = 19/78 (24%), Positives = 27/78 (34%), Gaps = 3/78 (3%)
Frame = -3
Query: 247 PKTTSHCITKATRNKNEPTEHNGVTSHPTPQPNPNCNKQHTEQTKPTRD---TTGSTPXE 77
P H + + PT+ H P P P +Q Q +P T +P
Sbjct: 598 PHLPPHTQQPSQIQEKPPTQEQ---PHYQPPPPPQHQQQSQSQPQPPHQPQHTQNQSPQL 654
Query: 76 KTHPXAISXPXKRPNRAP 23
P S P +P+ AP
Sbjct: 655 AQLPPHHSNPPAKPHGAP 672
>TP0001 TP0001 COG0593 [L] ATPase involved in DNA replication
initiation
Length = 464
Score = 32.7 bits (73), Expect = 1.4
Identities = 22/81 (27%), Positives = 35/81 (43%), Gaps = 8/81 (9%)
Frame = -1
Query: 510 HAVALLFAVYSNTPH---RPPQHGRTSPGPTPPARPILRRNHKARAQTPPTQQNHITQEQ 340
H + LLFAV TPH PP+H T PA +++ T +N ++ E+
Sbjct: 74 HPIKLLFAVKKGTPHGNTAPPKHVHTYLEKNSPAEVPSKKSFHPDLNRDYTFENFVSGEE 133
Query: 339 KTTQHN-----TQQPTTEAHP 292
H+ ++ P T +P
Sbjct: 134 TKFSHSAAISVSKNPGTSYNP 154
>CDA9099 CDa9099 COG1112 [L] Superfamily I DNA and RNA helicases and
helicase subunits
Length = 2018
Score = 30.8 bits (68), Expect = 5.2
Identities = 20/76 (26%), Positives = 32/76 (41%), Gaps = 3/76 (3%)
Frame = -3
Query: 247 PKTTSHCITKATRNKNEPTEHNGVTSHPTP---QPNPNCNKQHTEQTKPTRDTTGSTPXE 77
P +++ + + + N+ T+ N P P QP P Q + PTR T S P
Sbjct: 1882 PDYSANNLQSSAKQSNKDTKSNTTDDAPKPYINQPKP----QGDPKNGPTRSGTISKPST 1937
Query: 76 KTHPXAISXPXKRPNR 29
+ P S +PN+
Sbjct: 1938 TSTPAPTSSGTVKPNK 1953
>BH0276 BH0276 COG0577 [R] ABC-type transport systems, involved in
lipoprotein release,
Length = 541
Score = 30.8 bits (68), Expect = 5.2
Identities = 20/65 (30%), Positives = 31/65 (46%), Gaps = 3/65 (4%)
Frame = +1
Query: 1144 FVATRAFFIPVTFSLTFTFAYLGL*YFVLHRHSAGQQESWHSVRS---QKVXIFVHITRN 1314
FV FFI SL F A + + YF L AG++E + +R + V +TR
Sbjct: 419 FVKRIMFFIGFMLSLLFLSAAMSILYFYLQTSLAGEKEKYLGIRKIGLSMKEMSVIVTRE 478
Query: 1315 IYIML 1329
+ I++
Sbjct: 479 LSILI 483
>CDA8969 CDa8969 COG0470 [L] ATPase involved in DNA replication
Length = 897
Score = 30.0 bits (66), Expect = 8.9
Identities = 19/83 (22%), Positives = 32/83 (37%)
Frame = -3
Query: 895 TSNSRRGKPASTTRGINT*QMRYTSHDTVISGTQTSTQNRDDSSSCCELIKPHSNQK*NA 716
T N K ST++ Y + V++GT +NR S + ++ + + +
Sbjct: 41 TGNQNTKKNTSTSKIRKDKDQSYGGDEKVVNGTTEKLENRPTSLTSKDIFRIYREKDKQK 100
Query: 715 YTLCTPQHNKCNTPRHHKHFQIP 647
YTL Q + H H P
Sbjct: 101 YTLPDDQEPPVHHQPHETHQAFP 123
>SLR0744 slr0744 COG0532 [J] Translation initiation factor 2
(GTPase)
Length = 1001
Score = 30.0 bits (66), Expect = 8.9
Identities = 18/60 (30%), Positives = 26/60 (43%), Gaps = 1/60 (1%)
Frame = -1
Query: 468 HRPPQHGRTSPG-PTPPARPILRRNHKARAQTPPTQQNHITQEQKTTQHNTQQPTTEAHP 292
H+ + G SP PTPP RP L+ PP + + QK + + P + A P
Sbjct: 88 HKQEKSGGPSPARPTPPPRPKLQAPKAPTPPQPPVAKASAPKIQKQEEPAQEAPKSVAPP 147
Database: COGs
Posted date: Jan 30, 2002 4:01 PM
Number of letters in database: 26,228,252
Number of sequences in database: 78,847
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 56762639
Number of Sequences: 78847
Number of extensions: 1122300
Number of successful extensions: 3143
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 2935
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3117
length of query: 444
length of database: 26,228,252
effective HSP length: 49
effective length of query: 394
effective length of database: 22,364,749
effective search space: 8811711106
effective search space used: 8811711106
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 66 (30.0 bits)