The query sequence for this search has been filtered. Filtering
eliminates low complexity regions that commonly give spuriously high
scores that reflect compositional bias rather than significant
position-by-position alignment. Filtering can eliminate these potentially
confounding matches (e.g., hits against proline-rich regions or poly-A
tails) from the blast reports, leaving regions whose blast statistics
reflect the specificity of their pairwise alignment.
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FgrNT3_0_E01.seq Fgr_0_E01 LENGTH:261bp ; DIRECTION:5 ;
CLONE:Fgr_0_E01 ; CLONELIB: n/a ; TISSUE:
(261 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF
907,641 sequences; 284,333,007 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AAG09197.1|AF175209_1 (AF175209) Indian hedgehog protein... 31 3.2
ref|XP_058767.2| (XM_058767) similar to hemoglobin, zeta [H... 30 4.2
sp|P97812|IHH_MOUSE INDIAN HEDGEHOG PROTEIN PRECURSOR (IHH)... 29 9.5
ref|NP_034674.1| (NM_010544) Indian hedgehog homolog, (Dros... 29 9.5
pir||C49425 Indian hedgehog protein - mouse (fragment) 29 9.5
emb|CAA53923.1| (X76291) indian hedgehog [Mus musculus] 29 9.5
>gb|AAG09197.1|AF175209_1 (AF175209) Indian hedgehog protein [Rattus norvegicus]
Length = 125
Score = 30.8 bits (68), Expect = 3.2
Identities = 15/27 (55%), Positives = 17/27 (62%)
Frame = +1
Query: 1 PRRLFQAPLRILFISDNMAEPIRGKRA 81
PRRL P +LFI+DN AEP RA
Sbjct: 50 PRRLALTPAHLLFIADNHAEPAARFRA 76
>ref|XP_058767.2| (XM_058767) similar to hemoglobin, zeta [Homo sapiens]
Length = 115
Score = 30.4 bits (67), Expect = 4.2
Identities = 22/62 (35%), Positives = 26/62 (41%)
Frame = +3
Query: 3 KTFIPSASTHSFHIRQYGRAHPWKACSGRRGSYASEHSRNRRSYVFSR*GARCL*HQGGG 182
KT+ P H +R RA GRRG EH R+RR V + AR H G
Sbjct: 55 KTYFPHFDLHPGSVRAVARAR--LQGGGRRGRRGEEHRRHRRRPVQAERAARL--HPARG 110
Query: 183 SG 188
G
Sbjct: 111 PG 112
>sp|P97812|IHH_MOUSE INDIAN HEDGEHOG PROTEIN PRECURSOR (IHH) (HHG-2)
Length = 411
Score = 29.3 bits (64), Expect = 9.5
Identities = 14/27 (51%), Positives = 16/27 (58%)
Frame = +1
Query: 1 PRRLFQAPLRILFISDNMAEPIRGKRA 81
PRRL P +LFI+DN EP RA
Sbjct: 266 PRRLALTPAHLLFIADNHTEPAAHFRA 292
>ref|NP_034674.1| (NM_010544) Indian hedgehog homolog, (Drosophila) [Mus musculus]
gb|AAB49692.1| (U85610) Indian hedgehog protein [Mus musculus]
Length = 449
Score = 29.3 bits (64), Expect = 9.5
Identities = 14/27 (51%), Positives = 16/27 (58%)
Frame = +1
Query: 1 PRRLFQAPLRILFISDNMAEPIRGKRA 81
PRRL P +LFI+DN EP RA
Sbjct: 304 PRRLALTPAHLLFIADNHTEPAAHFRA 330
>pir||C49425 Indian hedgehog protein - mouse (fragment)
Length = 336
Score = 29.3 bits (64), Expect = 9.5
Identities = 14/27 (51%), Positives = 16/27 (58%)
Frame = +1
Query: 1 PRRLFQAPLRILFISDNMAEPIRGKRA 81
PRRL P +LFI+DN EP RA
Sbjct: 191 PRRLALTPAHLLFIADNHTEPAAHFRA 217
>emb|CAA53923.1| (X76291) indian hedgehog [Mus musculus]
Length = 336
Score = 29.3 bits (64), Expect = 9.5
Identities = 14/27 (51%), Positives = 16/27 (58%)
Frame = +1
Query: 1 PRRLFQAPLRILFISDNMAEPIRGKRA 81
PRRL P +LFI+DN EP RA
Sbjct: 191 PRRLALTPAHLLFIADNHTEPAAHFRA 217
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF
Posted date: Mar 29, 2002 2:16 AM
Number of letters in database: 284,333,007
Number of sequences in database: 907,641
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.267 0.0410 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 137,072,073
Number of Sequences: 907641
Number of extensions: 2456212
Number of successful extensions: 6710
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 6488
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6703
length of database: 284,333,007
effective HSP length: 62
effective length of database: 228,059,265
effective search space used: 5473422360
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)