The query sequence for this search has been filtered. Filtering
eliminates low complexity regions that commonly give spuriously high
scores that reflect compositional bias rather than significant
position-by-position alignment. Filtering can eliminate these potentially
confounding matches (e.g., hits against proline-rich regions or poly-A
tails) from the blast reports, leaving regions whose blast statistics
reflect the specificity of their pairwise alignment.
BLASTX 2.2.1 [Apr-13-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Fgr-S_8_A11_T7.seq Fgr-S_8_A11_T7 0 0 0 1 268
(267 letters)
Database: nr
722,277 sequences; 228,742,487 total letters
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAA15117.1| (D90787) Respiratory nitrate reductase 2 a ... 30 5.8
sp|P19319|NARZ_ECOLI RESPIRATORY NITRATE REDUCTASE 2 ALPHA ... 30 5.8
gb|AAG56302.1|AE005359_2 (AE005359) cryptic nitrate reducta... 30 5.8
pir||S11427 narZ protein - Escherichia coli 30 5.8
emb|CAA34964.1| (X17110) narZ product [Escherichia coli] 30 5.8
emb|CAC09479.1| (AL442113) hypothetical protein [Oryza sativa] 29 7.6
pir||H83158 Fe(III) dicitrate transport protein FecA PA3901... 29 7.6
emb|CAC30418.1| (AL583922) possible ribonuclease [Mycobacte... 29 7.6
>dbj|BAA15117.1| (D90787) Respiratory nitrate reductase 2 a chain (EC 1.7.99.4).
[Escherichia coli]
Length = 1245
Score = 29.6 bits (65), Expect = 5.8
Identities = 24/81 (29%), Positives = 36/81 (43%), Gaps = 2/81 (2%)
Frame = -2
Query: 254 RGQPSKQMYNQDDGSGCSG--YGVSDNIDAEDLDGSGETGEDEWQIL*MKGKFDFSVRLN 81
+G Q Y SG G G SD I E++ EWQ ++GK D V L+
Sbjct: 721 KGHEYMQKYLLGTESGIQGEELGASDGIKPEEV---------EWQTAAIEGKLDLLVTLD 771
Query: 80 LHMTTRWVNAPVCEVNTSTTWCE 12
M++ + + + V + TW E
Sbjct: 772 FRMSSTCLFSDI--VLPTATWYE 792
>sp|P19319|NARZ_ECOLI RESPIRATORY NITRATE REDUCTASE 2 ALPHA CHAIN
pir||G64899 nitrate reductase (EC 1.7.99.4) 2 alpha chain - Escherichia coli
gb|AAC74550.1| (AE000243) cryptic nitrate reductase 2, alpha subunit [Escherichia
coli K12]
Length = 1246
Score = 29.6 bits (65), Expect = 5.8
Identities = 24/81 (29%), Positives = 36/81 (43%), Gaps = 2/81 (2%)
Frame = -2
Query: 254 RGQPSKQMYNQDDGSGCSG--YGVSDNIDAEDLDGSGETGEDEWQIL*MKGKFDFSVRLN 81
+G Q Y SG G G SD I E++ EWQ ++GK D V L+
Sbjct: 722 KGHEYMQKYLLGTESGIQGEELGASDGIKPEEV---------EWQTAAIEGKLDLLVTLD 772
Query: 80 LHMTTRWVNAPVCEVNTSTTWCE 12
M++ + + + V + TW E
Sbjct: 773 FRMSSTCLFSDI--VLPTATWYE 793
>gb|AAG56302.1|AE005359_2 (AE005359) cryptic nitrate reductase 2, alpha subunit [Escherichia
coli O157:H7 EDL933]
dbj|BAB35494.1| (AP002557) cryptic nitrate reductase 2 alpha subunit [Escherichia
coli O157:H7]
Length = 1246
Score = 29.6 bits (65), Expect = 5.8
Identities = 24/81 (29%), Positives = 36/81 (43%), Gaps = 2/81 (2%)
Frame = -2
Query: 254 RGQPSKQMYNQDDGSGCSG--YGVSDNIDAEDLDGSGETGEDEWQIL*MKGKFDFSVRLN 81
+G Q Y SG G G SD I E++ EWQ ++GK D V L+
Sbjct: 722 KGHEYMQKYLLGTESGIQGEELGASDGIKPEEV---------EWQTAAIEGKLDLLVTLD 772
Query: 80 LHMTTRWVNAPVCEVNTSTTWCE 12
M++ + + + V + TW E
Sbjct: 773 FRMSSTCLFSDI--VLPTATWYE 793
>pir||S11427 narZ protein - Escherichia coli
Length = 1246
Score = 29.6 bits (65), Expect = 5.8
Identities = 24/81 (29%), Positives = 36/81 (43%), Gaps = 2/81 (2%)
Frame = -2
Query: 254 RGQPSKQMYNQDDGSGCSG--YGVSDNIDAEDLDGSGETGEDEWQIL*MKGKFDFSVRLN 81
+G Q Y SG G G SD I E++ EWQ ++GK D V L+
Sbjct: 722 KGHEYMQKYLLGTESGIQGEELGASDGIKPEEV---------EWQTAAIEGKLDLLVTLD 772
Query: 80 LHMTTRWVNAPVCEVNTSTTWCE 12
M++ + + + V + TW E
Sbjct: 773 FRMSSTCLFSDI--VLPTATWYE 793
>emb|CAA34964.1| (X17110) narZ product [Escherichia coli]
Length = 1246
Score = 29.6 bits (65), Expect = 5.8
Identities = 24/81 (29%), Positives = 36/81 (43%), Gaps = 2/81 (2%)
Frame = -2
Query: 254 RGQPSKQMYNQDDGSGCSG--YGVSDNIDAEDLDGSGETGEDEWQIL*MKGKFDFSVRLN 81
+G Q Y SG G G SD I E++ EWQ ++GK D V L+
Sbjct: 722 KGHEYMQKYLLGTESGIQGEELGASDGIKPEEV---------EWQTAAIEGKLDLLVTLD 772
Query: 80 LHMTTRWVNAPVCEVNTSTTWCE 12
M++ + + + V + TW E
Sbjct: 773 FRMSSTCLFSDI--VLPTATWYE 793
>emb|CAC09479.1| (AL442113) hypothetical protein [Oryza sativa]
Length = 536
Score = 29.3 bits (64), Expect = 7.6
Identities = 17/46 (36%), Positives = 22/46 (46%)
Frame = -2
Query: 260 RARGQPSKQMYNQDDGSGCSGYGVSDNIDAEDLDGSGETGEDEWQI 123
R R PS + DD G N DA+D G G+ GEDE ++
Sbjct: 444 RRRRTPSYGHDHDDDDDNYDG-----NADADDNGGGGDDGEDEEEV 484
>pir||H83158 Fe(III) dicitrate transport protein FecA PA3901 [imported] -
Pseudomonas aeruginosa (strain PAO1)
gb|AAG07288.1|AE004807_7 (AE004807) Fe(III) dicitrate transport protein FecA [Pseudomonas
aeruginosa]
Length = 784
Score = 29.3 bits (64), Expect = 7.6
Identities = 18/50 (36%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
Frame = -2
Query: 230 YNQDDGSGCSGYGVSDNIDAEDLDGSGETGE-DEWQIL*MKGKFDFSVRLN 81
YN D S Y + N ++E DGS TG W + +G +DF +LN
Sbjct: 692 YNLDGSFQTSQYADNANTESESADGS--TGRIAGWMVWSARGTYDFGPQLN 740
>emb|CAC30418.1| (AL583922) possible ribonuclease [Mycobacterium leprae]
Length = 924
Score = 29.3 bits (64), Expect = 7.6
Identities = 17/45 (37%), Positives = 22/45 (48%), Gaps = 2/45 (4%)
Frame = -2
Query: 266 RGRARGQPSKQMYNQDDGSGCSGYGVSDNIDAEDLDGSGET--GEDE 132
RGR RG+ DD G S++ D +D D S +T G DE
Sbjct: 160 RGRGRGEQGGSDVQSDDVDGAVDSTESEDFDGQDFDESEDTEIGYDE 206
Database: nr
Posted date: Jul 23, 2001 8:52 PM
Number of letters in database: 228,742,487
Number of sequences in database: 722,277
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.267 0.0410 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 118,238,011
Number of Sequences: 722277
Number of extensions: 2171467
Number of successful extensions: 4763
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 4634
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4755
length of database: 228,742,487
effective HSP length: 64
effective length of database: 182,516,759
effective search space used: 4380402216
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)