(Linear) MAP of: ./Fgr-S_1_A04_T7.seq check: 7652 from: 1 to: 982 Fgr-S_1_A04_T7 982 127 541 ABI Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * July 30, 2001 22:34 .. BssHII ApaI | SmaI BsaXI BseRI SnaBI | | | | | | CGGGCCCAGCGCGCCCGGCCCGGGGCNGGGCCGAGGGGAGGAGGCCNGGTGNTTTTGANNAGNAAGCNNTTGNAGTNAGTGACATACGTATTGTGATGCT 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 GCCCGGGTCGCGCGGGCCGGGCCCCGNCCCGGCTCCCCTCCTCCGGNCCACNAAAACTNNTCNTTCGNNAACNTCANTCACTGTATGCATAACACTACGA a R A Q R A R P G A G P R G G G ? V ? L ? ? K ? L ? ? V T Y V L * C Y - b G P S A P G P G ? G R G E E A ? ? F * ? ? S ? ? S ? * H T Y C D A - c G P A R P A R G ? A E G R R P G ? F ? ? ? A ? ? V S D I R I V M L - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d P G A R G A R P ? A S P L L G P ? K S ? ? A ? ? T L S M R I T I S - e P G L A G P G P ? P R P S S A ? H ? Q ? ? L ? Q L ? H C V Y Q S A - f A W R A R G P A P G L P P P ? T ? K ? L ? ? ? ? ? T V Y T N H H - EcoRI Bce83I EagI BsaBI PstI|Bpu1102I EagI | HindIII | | || | | | | ACNANNCGGCCGCCAGTGTGATCGGAATCTGCAGAATTCGGCTTAGCGTGGTCGCGGCCGAGGTACAAATCTACTCAAGTTACAAGCTTGGACTCCATCA 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 TGNTNNGCCGGCGGTCACACTAGCCTTAGACGTCTTAAGCCGAATCGCACCAGCGCCGGCTCCATGTTTAGATGAGTTCAATGTTCGAACCTGAGGTAGT a ? ? A A S V I G I C R I R L S V V A A E V Q I Y S S Y K L G L H H - b T ? R P P V * S E S A E F G L A W S R P R Y K S T Q V T S L D S I I - c ? ? G R Q C D R N L Q N S A * R G R G R G T N L L K L Q A W T P S - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d ? ? P R W H S R F R C F E A * R P R P R P V F R S L N C A Q V G D - e V ? R G G T H D S D A S N P K A H D R G L Y L D V * T V L K S E M - f * ? ? A A L T I P I Q L I R S L T T A A S T C I * E L * L S P S W * - XhoI XbaI DraIII ApaI | | | | TAGGTGTTACTCGAGACTTGATCTCTATTCTAGAACTCTCTAGGCGATTTCACCGGGTGAGTAAGCAACATCAGTCGGGCCCAAAGAACAACCTAAACGG 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 ATCCACAATGAGCTCTGAACTAGAGATAAGATCTTGAGAGATCCGCTAAAGTGGCCCACTCATTCGTTGTAGTCAGCCCGGGTTTCTTGTTGGATTTGCC a R C Y S R L D L Y S R T L * A I S P G E * A T S V G P K E Q P K R - b G V T R D L I S I L E L S R R F H R V S K Q H Q S G P K N N L N G - c * V L L E T * S L F * N S L G D F T G * V S N I S R A Q R T T * T V - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d Y T N S S V Q D R N * F E R P S K V P H T L L M L R A W L V V * V - e M P T V R S K I E I R S S E L R N * R T L L C C * D P G F F L R F P - f L H * E L S S R * E L V R * A I E G P S Y A V D T P G L S C G L R - SexAI BpmI BstEII HgiEII | | | | TGCGTGAATGTTCCTTTTGCTCCTTGCACAAAGATGACCAGGTTCACATTACATCAAGCAATCCAAAACCCACCTCCAGTGAGGTAACCATCACCATCCT 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 ACGCACTTACAAGGAAAACGAGGAACGTGTTTCTACTGGTCCAAGTGTAATGTAGTTCGTTAGGTTTTGGGTGGAGGTCACTCCATTGGTAGTGGTAGGA a C V N V P F A P C T K M T R F T L H Q A I Q N P P P V R * P S P S S - b A * M F L L L L A Q R * P G S H Y I K Q S K T H L Q * G N H H H P - c R E C S F C S L H K D D Q V H I T S S N P K P T S S E V T I T I L - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d T R S H E K Q E K C L S S W T * M V D L L G F G V E L S T V M V M R - e A H I N R K S R A C L H G P E C * M L C D L V W R W H P L W * W G - f H T F T G K A G Q V F I V L N V N C * A I W F G G G T L Y G D G D - BsmI NcoI BsgI | | | CGGTGCCCTTCTTCTCTTGTGCATTCATGGATGATGAACTGCCTGCAAAACCATGGGACGTGCAGACGTTAGAACGAACTCGTAAAAACCTGAACATTGA 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 GCCACGGGAAGAAGAGAACACGTAAGTACCTACTACTTGACGGACGTTTTGGTACCCTGCACGTCTGCAATCTTGCTTGAGCATTTTTGGACTTGTAACT a V P F F S C A F M D D E L P A K P W D V Q T L E R T R K N L N I D - b R C P S S L V H S W M M N C L Q N H G T C R R * N E L V K T * T L I - c G A L L L L C I H G * * T A C K T M G R A D V R T N S * K P E H * - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d P A R R R K H M * P H H V A Q L V M P R A S T L V F E Y F G S C Q - e R H G E E R T C E H I I F Q R C F W P V H L R * F S S T F V Q V N - f E T G K K E Q A N M S S S S G A F G H S T C V N S R V R L F R F M S - EciI BspMI | SmaI | | HindIII SrfI | | EcoRI BstXI EagI SpeI BamHI SacI KpnI | | | | | | | | | | | | TTACCTGCCCGGGCGGACGCTCGAAAGCCGAATTCCAGCACACTGGCGGCCGTTACTAGTGGATCCGAGCTCGGTACCAAGCTTGGCGTAATCATGGTCA 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 AATGGACGGGCCCGCCTGCGAGCTTTCGGCTTAAGGTCGTGTGACCGCCGGCAATGATCACCTAGGCTCGAGCCATGGTTCGAACCGCATTAGTACCAGT a Y L P G R T L E S R I P A H W R P L L V D P S S V P S L A * S W S - b T C P G G R S K A E F Q H T G G R Y * W I R A R Y Q A W R N H G H - c L P A R A D A R K P N S S T L A A V T S G S E L G T K L G V I M V I - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d N G A R A S A R F G F E L V S A A T V L P D S S P V L S P T I M T - e I V Q G P P R E F A S N W C V P P R * * H I R A R Y W A Q R L * P * - f * R G P R V S S L R I G A C Q R G N S T S G L E T G L K A Y D H D - BsrBI | TAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGCGTAAAGCCTGGGGTGCCTAATGAGTGAGCT 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 ATCGACAAAGGACACACTTTAACAATAGGCGAGTGTTAAGGTGTGTTGTATGCTCGGCCTTCGTATTTCGCATTTCGGACCCCACGGATTACTCACTCGA a * L F P V * N C Y P L T I P H N I R A G S I K R K A W G A * * V S * - b S C F L C E I V I R S Q F H T T Y E P E A * S V K P G V P N E * A - c A V S C V K L L S A H N S T Q H T S R K H K A * S L G C L M S E L - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d M A T E Q T F N N D A * L E V C C V L R F C L A Y L R P H R I L S S - e L Q K R H S I T I R E C N W V V Y S G S A Y L T F G P T G L S H A - f Y S N G T H F Q * G S V I G C L M R A P L M F R L A Q P A * H T L - VspI VspI | | AACTCACATTAATTGCGTTGCGCTCACTGTCCGCTTTGCAGTCGGGAAACCTGTCGTGCCCGCTGCATTAATGAATCGGCCAACGCGCGGTGAGAAGCCG 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 TTGAGTGTAATTAACGCAACGCGAGTGACAGGCGAAACGTCAGCCCTTTGGACAGCACGGGCGACGTAATTACTTAGCCGGTTGCGCGCCACTCTTCGGC a L T L I A L R S L S A L Q S G N L S C P L H * * I G Q R A V R S R - b N S H * L R C A H C P L C S R E T C R A R C I N E S A N A R * E A G - c T H I N C V A L T V R F A V G K P V V P A A L M N R P T R G E K P - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d V * M L Q T A S V T R K A T P F G T T G A A N I F R G V R P S F G - e L E C * N R Q A * Q G S Q L R S V Q R A R Q M L S D A L A R H S A - f * S V N I A N R E S D A K C D P F R D H G S C * H I P W R A T L L R - ApaBI AlwNI| HpaI || | GTTGCGTAATGGGCAGACTCTGCGCTTCCTTAGTTAATGACTTGCTTCGCCTCGTCGGTCGGCTGGGGGGAACGGGTTAACCTTACTTCAAGGCCGGGAT 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 CAACGCATTACCCGTCTGAGACGCGAAGGAATCAATTACTGAACGAAGCGGAGCAGCCAGCCGACCCCCCTTGCCCAATTGGAATGAAGTTCCGGCCCTA a L R N G Q T L R F L S * * L A S P R R S A G G N G L T L L Q G R D - b C V M G R L C A S L V N D L L R L V G R L G G T G * P Y F K A G I - c V A * W A D S A L P * L M T C F A S S V G W G E R V N L T S R P G F - 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 d T A Y H A S E A S G * N I V Q K A E D T P Q P S R T L R V E L G P - e P Q T I P L S Q A E K T L S K S R R T P R S P P V P * G * K L A P I - f N R L P C V R R K R L * H S A E G R R D A P P F P N V K S * P R S - DrdII | TCCGGTTTTCCCAGGATCAGGGGAATACGCCGGGAAAAAACCTGTGAACCAAAAGGGCCACCAAAAGGCCAGGAACCGTAAA 901 ---------+---------+---------+---------+---------+---------+---------+---------+-- 982 AGGCCAAAAGGGTCCTAGTCCCCTTATGCGGCCCTTTTTTGGACACTTGGTTTTCCCGGTGGTTTTCCGGTCCTTGGCATTT a S G F P R I R G I R R E K T C E P K G P P K G Q E P * - b P V F P G S G E Y A G K K P V N Q K G H Q K A R N R K - c R F S Q D Q G N T P G K N L * T K R A T K R P G T V - 901 ---------+---------+---------+---------+---------+---------+---------+---------+-- 982 d N R N E W S * P F V G P F F R H V L L A V L L G P V T F - e G T K G P D P S Y A P F F G T F W F P W W F A L F R L - f E P K G L I L P I R R S F V Q S G F P G G F P W S G Y - Enzymes that do cut: AlwNI ApaI ApaBI BamHI Bce83I BpmI Bpu1102I BsaBI BsaXI BseRI BsgI BsmI BspMI BsrBI BssHII BstEII BstXI DraIII DrdII EagI EciI EcoRI HgiEII HindIII HpaI KpnI NcoI PstI SacI SexAI SmaI SnaBI SpeI SrfI VspI XbaI XhoI Enzymes that do not cut: AatII AceIII AflII AhdI ApaLI AscI AvrII BbsI BcgI BclI BfiI BglI BglII BplI Bpu10I BsaI BsbI BsiWI BsmBI BspEI BspGI BspLU11I BsrDI BsrGI BssSI Bst1107I Bsu36I ClaI DraI DrdI EarI Eco47III Eco57I EcoNI EcoRV FseI FspI MfeI MluI MscI NarI NdeI NgoAIV NheI NotI NruI NsiI NspV PacI Pfl1108I PflMI PinAI PmeI PmlI PshAI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SanDI SapI ScaI SfiI SgfI SgrAI SphI Sse8387I Sse8647I SspI StuI SwaI Tth111I XcmI XmnI