(Linear) MAP of: ./Fgr-S_1_A09_T7.seq check: 4689 from: 1 to: 887 Fgr-S_1_A09_T7 887 133 574 ABI Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * July 30, 2001 22:34 .. BcgI | AGGCCNNAAANTGGGACATTTAGTGGGACNTCCTNNCTNAGTAANGGCATGGNCATNCGNAGNNCGGGCTCGCACAGATGGTGTATCGGCNATATCTGGC 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 TCCGGNNTTTNACCCTGTAAATCACCCTGNAGGANNGANTCATTNCCGTACCNGTANGCNTCNNGCCCGAGCGTGTCTACCACATAGCCGNTATAGACCG a R P ? ? G T F S G T S ? L S ? G M ? ? R ? ? G S H R W C I G ? I W Q - b G ? K ? G H L V G ? P ? ? V ? A W ? ? ? ? R A R T D G V S A I S G - c A ? ? W D I * W D ? L ? ? * ? H G H ? ? ? G L A Q M V Y R ? Y L A - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d G ? ? P V N L P V ? ? ? L L P M ? M R L ? P E C L H H I P ? I Q - e A ? F ? S M * H S ? R ? ? Y ? C P ? ? ? ? P S A C I T Y R ? Y R A - f P ? ? ? P C K T P ? G ? ? T ? A H ? ? ? ? R A R V S P T D A ? D P - BcgI | AGCACATTCGGCATTAGACGTGGTGCGCGGGCCGAGGTACAACAACAGCAAAAGGGAAGCGTTTTTTACACAGGAATTACCCATCCTAATCCCGCCCTAA 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 TCGTGTAAGCCGTAATCTGCACCACGCGCCCGGCTCCATGTTGTTGTCGTTTTCCCTTCGCAAAAAATGTGTCCTTAATGGGTAGGATTAGGGCGGGATT a H I R H * T W C A G R G T T T A K G K R F L H R N Y P S * S R P N - b S T F G I R R G A R A E V Q Q Q Q K G S V F Y T G I T H P N P A L I - c A H S A L D V V R G P R Y N N S K R E A F F T Q E L P I L I P P * - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d C C M R C * V H H A P R P V V V A F P F R K K C L F * G D * D R G L - e A C E A N S T T R P G L Y L L L L L S A N K V C S N G M R I G G * - f L V N P M L R P A R A S T C C C C F P L T K * V P I V W G L G A R - PacI PacI | BssSI VspI | AceIII | BspEI SacI | | | | | | TTTAATTAATTAACTAAATTTCAAGAACAGCTCAACGCCACGCCACGAGCTTATCCGGAACAGAGCTCATCGGGGCAGAGGCACGGACACGCCCTTGCGT 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 AAATTAATTAATTGATTTAAAGTTCTTGTCGAGTTGCGGTGCGGTGCTCGAATAGGCCTTGTCTCGAGTAGCCCCGTCTCCGTGCCTGTGCGGGAACGCA a L I N * L N F K N S S T P R H E L I R N R A H R G R G T D T P L R - b * L I N * I S R T A Q R H A T S L S G T E L I G A E A R T R P C V - c F N * L T K F Q E Q L N A T P R A Y P E Q S S S G Q R H G H A L A S - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d K I L * S F K L F L E V G R W S S I R F L A * R P L P V S V G K R - e N L * N V L N * S C S L A V G R A * G S C L E D P C L C P C A R A - f I * N I L * I E L V A * R W A V L K D P V S S M P A S A R V R G Q T - EagI NotI BglI| BspMI|| SmaI ||| HindIII ApaBI SrfI |||BsrBI EcoRI BstXI EagI SpeI BamHI SacI KpnI | | | ||| | | | | | | | | | CGGCGTCCGCAGCCGTTGCACCTGCCCGGGCGGCCGCTCGAAAGCCGAATTCCAGCACACTGGCGGCCGTTACTAGTGGATCCGAGCTCGGTACCAAGCT 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 GCCGCAGGCGTCGGCAACGTGGACGGGCCCGCCGGCGAGCTTTCGGCTTAAGGTCGTGTGACCGCCGGCAATGATCACCTAGGCTCGAGCCATGGTTCGA a R R P Q P L H L P G R P L E S R I P A H W R P L L V D P S S V P S L - b G V R S R C T C P G G R S K A E F Q H T G G R Y * W I R A R Y Q A - c A S A A V A P A R A A A R K P N S S T L A A V T S G S E L G T K L - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d R R G C G N C R G P R G S S L R I G A C Q R G N S T S G L E T G L - e D A D A A T A G A R A A A R F G F E L V S A A T V L P D S S P V L S - f P T R L R Q V Q G P P R E F A S N W C V P P R * * H I R A R Y W A - BsrBI | TGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGG 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 ACCGCATTAGTACCAGTATCGACAAAGGACACACTTTAACAATAGGCGAGTGTTAAGGTGTGTTGTATGCTCGGCCTTCGTATTTCACATTTCGGACCCC a A * S W S * L F P V * N C Y P L T I P H N I R A G S I K C K A W G - b W R N H G H S C F L C E I V I R S Q F H T T Y E P E A * S V K P G V - c G V I M V I A V S C V K L L S A H N S T Q H T S R K H K V * S L G - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d K A Y D H D Y S N G T H F Q * G S V I G C L M R A P L M F H L A Q P - e P T I M T M A T E Q T F N N D A * L E V C C V L R F C L T Y L R P - f Q R L * P * L Q K R H S I T I R E C N W V V Y S G S A Y L T F G P - VspI PvuII VspI | | | TGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAA 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 ACGGATTACTCACTCGATTGAGTGTAATTAACGCAACGCGAGTGACGGGCGAAAGGTCAGCCCTTTGGACAGCACGGTCGACGTAATTACTTAGCCGGTT a A * * V S * L T L I A L R S L P A F Q S G N L S C Q L H * * I G Q - b P N E * A N S H * L R C A H C P L S S R E T C R A S C I N E S A N - c C L M S E L T H I N C V A L T A R F P V G K P V V P A A L M N R P T - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d A * H T L * S V N I A N R E S G A K W D P F R D H W S C * H I P W - e H R I L S S V * M L Q T A S V A R K G T P F G T T G A A N I F R G - f T G L S H A L E C * N R Q A * Q G S E L R S V Q R A L Q M L S D A L - EarI BsaXI SapI BsrBI | | | CGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTC 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 GCGCGCCCCTCTCCGCCAAACGCATAACCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAG a R A G R G G L R I G R S S A S S L T D S L R S V V R L R R A V S A H - b A R G E A V C V L G A L P L P R S L T R C A R S F G C G E R Y Q L - c R G E R R F A Y W A L F R F L A H * L A A L G R S A A A S G I S S - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d R A P L P P K R I P R E E A E E S V S E S R E T T R S R R A T D A - e V R P S L R N A Y Q A S K R K R A * Q S A A S P R E A A A L P I L E - f A R P S A T Q T N P A R G S G R E S V R Q A R D N P Q P S R Y * S - AceIII BspLU11I | | ACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCACGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGC 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 TGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTGCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCG a S K A V I R L S T E S G D N A R K N M * A K G Q Q K A R N R K K A - b T Q R R * Y G Y P Q N Q G I T H E R T C E Q K A S K R P G T V K R P - c L K G G N T V I H R I R G * R T K E H V S K R P A K G Q E P * K G - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d * E F A T I R N D V S D P S L A R F F M H A F P W C F A L F R L F A - e S L P P L V T I W L I L P Y R V F S C T L L L G A F P W S G Y F P - f V * L R Y Y P * G C F * P I V C S L V H S C F A L L L G P V T F L - Bce83I DrdI | | CGCGTTGCTGGCGTTTTTCCATAGGCTCCGNCCCCCTGACGAGCATGACAAAAATCGACGCTCAAGTCAGAAGTGGCGAAACCCGAA 801 ---------+---------+---------+---------+---------+---------+---------+---------+------- 887 GCGCAACGACCGCAAAAAGGTATCCGAGGCNGGGGGACTGCTCGTACTGTTTTTAGCTGCGAGTTCAGTCTTCACCGCTTTGGGCTT a A L L A F F H R L R P P D E H D K N R R S S Q K W R N P - b R C W R F S I G S ? P L T S M T K I D A Q V R S G E T R - c R V A G V F P * A P ? P * R A * Q K S T L K S E V A K P E - 801 ---------+---------+---------+---------+---------+---------+---------+---------+------- 887 d A N S A N K W L S R G G S S C S L F R R E L * F H R F G F - e R T A P T K G Y A G ? G Q R A H C F D V S L D S T A F G S - f G R Q Q R K E M P E ? G R V L M V F I S A * T L L P S V R - Enzymes that do cut: AceIII ApaBI BamHI Bce83I BcgI BglI BsaXI BspEI BspLU11I BspMI BsrBI BssSI BstXI DrdI EagI EarI EcoRI HindIII KpnI NotI PacI PvuII SacI SapI SmaI SpeI SrfI VspI Enzymes that do not cut: AatII AflII AhdI AlwNI ApaI ApaLI AscI AvrII BbsI BclI BfiI BglII BplI BpmI Bpu10I Bpu1102I BsaI BsaBI BsbI BseRI BsgI BsiWI BsmI BsmBI BspGI BsrDI BsrGI BssHII Bst1107I BstEII Bsu36I ClaI DraI DraIII DrdII EciI Eco47III Eco57I EcoNI EcoRV FseI FspI HgiEII HpaI MfeI MluI MscI NarI NcoI NdeI NgoAIV NheI NruI NsiI NspV Pfl1108I PflMI PinAI PmeI PmlI PshAI Psp1406I PstI PvuI RcaI RsrII SacII SalI SanDI ScaI SexAI SfiI SgfI SgrAI SnaBI SphI Sse8387I Sse8647I SspI StuI SwaI Tth111I XbaI XcmI XhoI XmnI