(Linear) MAP of: ./Fgr-S_1_A10_T7.seq check: 6611 from: 1 to: 902 Fgr-S_1_A10_T7 902 42 727 ABI Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * July 30, 2001 22:34 .. EagI NotI EcoRV NsiI BsrBI| BsaBI | EcoRI SanDI BsrDI |XhoI || BstXI | | PstI|Bpu1102I EagI | | | | || | | | || | | GGGAATGGAATTGGGACCCCNCTTAGATGCATTGCTCGAGCGGCCGCCAGTGTGATGGATATCTGCAGAATTCGGCTTAGCGTGGTCGCGGCCGAGGTCC 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 CCCTTACCTTAACCCTGGGGNGAATCTACGTAACGAGCTCGCCGGCGGTCACACTACCTATAGACGTCTTAAGCCGAATCGCACCAGCGCCGGCTCCAGG a G N G I G T P L R C I A R A A A S V M D I C R I R L S V V A A E V R - b G M E L G P ? L D A L L E R P P V * W I S A E F G L A W S R P R S - c E W N W D P ? * M H C S S G R Q C D G Y L Q N S A * R G R G R G P - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d F P I P V G ? L H M A R A A A L T I S I Q L I R S L T T A A S T - e S H F Q S G ? * I C Q E L P R W H S P Y R C F E A * R P R P R P G - f P I S N P G ? K S A N S S R G G T H H I D A S N P K A H D R G L D - NsiI ApaBI PvuII HpaI | | | | GCACTGGATATGGCAAGGGATATGCATTGGTTGAATATGGATGTTTTGAGGAAGCACAAACTGCAATCAGAAGTATGAATGGGACACAGCTGTTAACAAA 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 CGTGACCTATACCGTTCCCTATACGTAACCAACTTATACCTACAAAACTCCTTCGTGTTTGACGTTAGTCTTCATACTTACCCTGTGTCGACAATTGTTT a T G Y G K G Y A L V E Y G C F E E A Q T A I R S M N G T Q L L T K - b A L D M A R D M H W L N M D V L R K H K L Q S E V * M G H S C * Q R - c H W I W Q G I C I G * I W M F * G S T N C N Q K Y E W D T A V N K - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d R V P Y P L P Y A N T S Y P H K S S A C V A I L L I F P V C S N V F - e C Q I H C P I H M P Q I H I N Q P L V F Q L * F Y S H S V A T L L - f A S S I A L S I C Q N F I S T K L F C L S C D S T H I P C L Q * C - ApaLI BsmI Sse8647I BssSI | | | | | GACCATCCATGTTGATTGGGCATTCAACAGAGGTCCTATACAGAACGTCACGAGTGCACGGCCAGCACAGCGACGGTCTAGGACTCCACCTCGCAGGCTT 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 CTGGTAGGTACAACTAACCCGTAAGTTGTCTCCAGGATATGTCTTGCAGTGCTCACGTGCCGGTCGTGTCGCTGCCAGATCCTGAGGTGGAGCGTCCGAA a T I H V D W A F N R G P I Q N V T S A R P A Q R R S R T P P R R L - b P S M L I G H S T E V L Y R T S R V H G Q H S D G L G L H L A G L - c D H P C * L G I Q Q R S Y T E R H E C T A S T A T V * D S T S Q A C - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d V M W T S Q A N L L P G I C F T V L A R G A C R R D L V G G R L S - e S W G H Q N P M * C L D * V S R * S H V A L V A V T * S E V E C A - f L G D M N I P C E V S T R Y L V D R T C P W C L S P R P S W R A P K - AceIII StuI BsrDI | | | GCTGCCATGACATACTGAGAGGGAGCTGCTAGGCCTGCAATGTTGGGCCAGCCTTGGTCAGTGTTTTTTTGTAGTCGCCAGGGATTTGCTATGTCATAGA 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 CGACGGTACTGTATGACTCTCCCTCGACGATCCGGACGTTACAACCCGGTCGGAACCAGTCACAAAAAAACATCAGCGGTCCCTAAACGATACAGTATCT a A A M T Y * E G A A R P A M L G Q P W S V F F C S R Q G F A M S * I - b L P * H T E R E L L G L Q C W A S L G Q C F F V V A R D L L C H R - c C H D I L R G S C * A C N V G P A L V S V F L * S P G I C Y V I D - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d A A M V Y Q S P A A L G A I N P W G Q D T N K Q L R W P N A I D Y - e Q Q W S M S L P L Q * A Q L T P G A K T L T K K Y D G P I Q * T M S - f S G H C V S L S S S P R C H Q A L R P * H K K T T A L S K S H * L - BstEII NheI | | TTTTAGTCTTAACCGTCGTCATCTGGCACTAAGCGGTAACCGAACAATGAACCGGGTATGTATCGTCGAGAAACCGAGTCCTGATTCTGCTAGCCTGTAC 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 AAAATCAGAATTGGCAGCAGTAGACCGTGATTCGCCATTGGCTTGTTACTTGGCCCATACATAGCAGCTCTTTGGCTCAGGACTAAGACGATCGGACATG a L V L T V V I W H * A V T E Q * T G Y V S S R N R V L I L L A C T - b F * S * P S S S G T K R * P N N E P G M Y R R E T E S * F C * P V P - c F S L N R R H L A L S G N R T M N R V C I V E K P S P D S A S L Y - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d I K T K V T T M Q C * A T V S C H V P Y T D D L F R T R I R S A Q V - e K L R L R R * R A S L P L R V I F R T H I T S F G L G S E A L R Y - f N * D * G D D D P V L R Y G F L S G P I Y R R S V S D Q N Q * G T - EagI NotI BglI| BspMI|| SmaI ||| HindIII SrfI |||BsrBI EcoRI BstXI EagI SpeI BamHI SacI KpnI | | ||| | | | | | | | | | CTGCCCGGGCGGCCGCTCGAAAGCCGAATTCCAGCACACTGGCGGCCGTTACTAGTGGATCCGAGCTCGGTACCAAGCTTGGCGTAATCATGGTCATAGC 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 GACGGGCCCGCCGGCGAGCTTTCGGCTTAAGGTCGTGTGACCGCCGGCAATGATCACCTAGGCTCGAGCCATGGTTCGAACCGCATTAGTACCAGTATCG a C P G G R S K A E F Q H T G G R Y * W I R A R Y Q A W R N H G H S - b A R A A A R K P N S S T L A A V T S G S E L G T K L G V I M V I A - c L P G R P L E S R I P A H W R P L L V D P S S V P S L A * S W S * L - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d Q G P P R E F A S N W C V P P R * * H I R A R Y W A Q R L * P * L - e R G P R G S S L R I G A C Q R G N S T S G L E T G L K A Y D H D Y - f G A R A A A R F G F E L V S A A T V L P D S S P V L S P T I M T M A - BsrBI | TGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACT 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 ACAAAGGACACACTTTAACAATAGGCGAGTGTTAAGGTGTGTTGTATGCTCGGCCTTCGTATTTCACATTTCGGACCCCACGGATTACTCACTCGATTGA a C F L C E I V I R S Q F H T T Y E P E A * S V K P G V P N E * A N S - b V S C V K L L S A H N S T Q H T S R K H K V * S L G C L M S E L T - c F P V * N C Y P L T I P H N I R A G S I K C K A W G A * * V S * L - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d Q K R H S I T I R E C N W V V Y S G S A Y L T F G P T G L S H A L - e S N G T H F Q * G S V I G C L M R A P L M F H L A Q P A * H T L * S - f T E Q T F N N D A * L E V C C V L R F C L T Y L R P H R I L S S V - VspI PvuII VspI | | | CACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAAGGCCGTTT 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 GTGTAATTAACGCAACGCGAGTGACGGGCGAAAGGTCAGCCCTTTGGACAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCCCTCTTCCGGCAAA a H * L R C A H C P L S S R E T C R A S C I N E S A N A R G E G R F - b H I N C V A L T A R F P V G K P V V P A A L M N R P T R G E K A V L - c T L I A L R S L P A F Q S G N L S C Q L H * * I G Q R A G R R P F - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d E C * N R Q A * Q G S E L R S V Q R A L Q M L S D A L A R P S P R K - e V N I A N R E S G A K W D P F R D H W S C * H I P W R A P L L G N - f * M L Q T A S V A R K G T P F G T T G A A N I F R G V R P S F A T - EarI SapI AceIII | | TGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTTCGGTCGTTCGGCTGCGGCGAAGCGTATCAGCTCACTCAAAAGCGGTAATAC 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 ACGCATAACCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAAGCCAGCAAGCCGACGCCGCTTCGCATAGTCGAGTGAGTTTTCGCCATTATG a A Y W A L F R F L A H * L A A L R S F G C G E A Y Q L T Q K R * Y - b R I G R S S A S S L T D S L R F G R S A A A K R I S S L K S G N T - c C V L G A L P L P R S L T R C A S V V R L R R S V S A H S K A V I R - 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 d A Y Q A S K R K R A * Q S A A S R D N P Q P S A Y * S V * F R Y Y - e Q T N P A R G S G R E S V R Q A E T T R S R R L T D A * E F A T I - f K R I P R E E A E E S V S E S R K P R E A A A F R I L E S L L P L V - GA 901 -- 902 CT a - b - c - 901 -- 902 d S - e R - f - Enzymes that do cut: AceIII ApaBI ApaLI BamHI BglI Bpu1102I BsaBI BsmI BspMI BsrBI BsrDI BssSI BstEII BstXI EagI EarI EcoRI EcoRV HindIII HpaI KpnI NheI NotI NsiI PstI PvuII SacI SanDI SapI SmaI SpeI SrfI Sse8647I StuI VspI XhoI Enzymes that do not cut: AatII AflII AhdI AlwNI ApaI AscI AvrII BbsI Bce83I BcgI BclI BfiI BglII BplI BpmI Bpu10I BsaI BsaXI BsbI BseRI BsgI BsiWI BsmBI BspEI BspGI BspLU11I BsrGI BssHII Bst1107I Bsu36I ClaI DraI DraIII DrdI DrdII EciI Eco47III Eco57I EcoNI FseI FspI HgiEII MfeI MluI MscI NarI NcoI NdeI NgoAIV NruI NspV PacI Pfl1108I PflMI PinAI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SexAI SfiI SgfI SgrAI SnaBI SphI Sse8387I SspI SwaI Tth111I XbaI XcmI XmnI