(Linear) MAP of: ./Fgr-S_1_A11_T7.seq check: 4758 from: 1 to: 892 Fgr-S_1_A11_T7 892 27 655 ABI Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * July 30, 2001 22:34 .. EagI NotI EcoRV BsrBI| BsaBI | EcoRI ApaI XhoI || BstXI | | PstI|Bpu1102I EagI | | || | | | || | | AGGATTGAATTGGGCCCTCTANATGCTGCTCGAGCGGCCGCCAGTGTGATGGATATCTGCAGAATTCGGCTTAGCGTGGTCGCGGCCGAGGTACAAGGCA 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 TCCTAACTTAACCCGGGAGATNTACGACGAGCTCGCCGGCGGTCACACTACCTATAGACGTCTTAAGCCGAATCGCACCAGCGCCGGCTCCATGTTCCGT a R I E L G P L ? A A R A A A S V M D I C R I R L S V V A A E V Q G M - b G L N W A L ? M L L E R P P V * W I S A E F G L A W S R P R Y K A - c D * I G P S ? C C S S G R Q C D G Y L Q N S A * R G R G R G T R H - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d S Q I P G E ? H Q E L P R W H S P Y R C F E A * R P R P R P V L C - e P N F Q A R * ? S S S R G G T H H I D A S N P K A H D R G L Y L A - f I S N P G R ? A A R A A A L T I S I Q L I R S L T T A A S T C P - FseI NgoAIV EagI | NgoAIV | | NsiI | | | SphI | | | | NsiI | | | | | BsaBI SphI | | | | | | DrdII DraI EcoRV | | | | | | | | | | | | TGCATGCATGGGCCGGCCGGCAAAACATATATAGGAACCAAGAAAGAAACCTCGAATTTAAACTAACAGATATCAATCCGGTATATATATAGCTTTTCCC 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 ACGTACGTACCCGGCCGGCCGTTTTGTATATATCCTTGGTTCTTTCTTTGGAGCTTAAATTTGATTGTCTATAGTTAGGCCATATATATATCGAAAAGGG a H A W A G R Q N I Y R N Q E R N L E F K L T D I N P V Y I * L F P - b C M H G P A G K T Y I G T K K E T S N L N * Q I S I R Y I Y S F S R - c A C M G R P A K H I * E P R K K P R I * T N R Y Q S G I Y I A F P - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d A H M P R G A F C I Y S G L F F G R I * V L L Y * D P I Y I A K G - e H M C P G A P L V Y I P V L F S V E F K F * C I D I R Y I Y L K E - f M C A H A P R C F M Y L F W S L F R S N L S V S I L G T Y I Y S K G - HgiEII EciI Eco57I | | | GACCATGCTGGGTGCTTCTCCTATTCCGGCGGAGCGATATGTCTGAAGTTGCCGTTGATTTCTTCACGGAAGCGGACGGACTTGGGGCCGGGGCAAACTC 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 CTGGTACGACCCACGAAGAGGATAAGGCCGCCTCGCTATACAGACTTCAACGGCAACTAAAGAAGTGCCTTCGCCTGCCTGAACCCCGGCCCCGTTTGAG a T M L G A S P I P A E R Y V * S C R * F L H G S G R T W G R G K L - b P C W V L L L F R R S D M S E V A V D F F T E A D G L G A G A N S - c D H A G C F S Y S G G A I C L K L P L I S S R K R T D L G P G Q T Q - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d S W A P H K E * E P P A I H R F N G N I E E R F R V S K P G P C V - e R G H Q T S R R N R R L S I D S T A T S K K V S A S P S P A P A F E - f V M S P A E G I G A S R Y T Q L Q R Q N R * P L P R V Q P R P L S - BclI PinAI RcaI | | | AACTAAAAGATGATCAGTACCGGTGTACTATCTTGGACCGAAAATTAGCGTTGCTAGGTTTTTCTTGTCGATCTGATGACTCAATGGCTAGTCATGATCC 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 TTGATTTTCTACTAGTCATGGCCACATGATAGAACCTGGCTTTTAATCGCAACGATCCAAAAAGAACAGCTAGACTACTGAGTTACCGATCAGTACTAGG a N * K M I S T G V L S W T E N * R C * V F L V D L M T Q W L V M I R - b T K R * S V P V Y Y L G P K I S V A R F F L S I * * L N G * S * S - c L K D D Q Y R C T I L D R K L A L L G F S C R S D D S M A S H D P - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d * S F S S * Y R H V I K S R F N A N S P K E Q R D S S E I A L * S G - e V L L H D T G T Y * R P G F I L T A L N K K D I Q H S L P * D H D - f L * F I I L V P T S D Q V S F * R Q * T K R T S R I V * H S T M I - BsaI BssSI AceIII BpmI | Bpu1102I | DrdII | PstI | | | | | | | GCGCCATGCCAGTCCGGTCTCCCCAGCGTGAACTCCAGGCTCGGGCTGCTCAGCTCGTGGTTCGACACTTGCGAGCCGGTTGATCTGCAGGGCTCCATGT 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 CGCGGTACGGTCAGGCCAGAGGGGTCGCACTTGAGGTCCGAGCCCGACGAGTCGAGCACCAAGCTGTGAACGCTCGGCCAACTAGACGTCCCGAGGTACA a A M P V R S P Q R E L Q A R A A Q L V V R H L R A G * S A G L H V - b A P C Q S G L P S V N S R L G L L S S W F D T C E P V D L Q G S M F - c R H A S P V S P A * T P G S G C S A R G S T L A S R L I C R A P C - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d R W A L G T E G A H V G P E P Q E A R P E V S A L R N I Q L A G H - e A G H W D P R G L T F E L S P S S L E H N S V Q S G T S R C P E M - f R A M G T R D G W R S S W A R A A * S T T R C K R A P Q D A P S W T - EagI NotI BglI| BspMI|| SmaI ||| SrfI ||| HindIII BbsI | |||BsrBI EcoRI BstXI EagI SpeI BamHI SacI KpnI | | | ||| | | | | | | | | | TCTCCATTGGAGAAGACCTGCCCGGGCGGCCGCTCGAAAGCCGAATTCCAGCACACTGGCGGCCGTTACTAGTGGATCCGAGCTCGGTACCAAGCTTGGC 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 AGAGGTAACCTCTTCTGGACGGGCCCGCCGGCGAGCTTTCGGCTTAAGGTCGTGTGACCGCCGGCAATGATCACCTAGGCTCGAGCCATGGTTCGAACCG a L H W R R P A R A A A R K P N S S T L A A V T S G S E L G T K L G - b S I G E D L P G R P L E S R I P A H W R P L L V D P S S V P S L A - c S P L E K T C P G G R S K A E F Q H T G G R Y * W I R A R Y Q A W R - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d E G N S F V Q G P P R E F A S N W C V P P R * * H I R A R Y W A Q - e N E M P S S R G P R G S S L R I G A C Q R G N S T S G L E T G L K A - f R W Q L L G A R A A A R F G F E L V S A A T V L P D S S P V L S P - BsrBI | GTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCC 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 CATTAGTACCAGTATCGACAAAGGACACACTTTAACAATAGGCGAGTGTTAAGGTGTGTTGTATGCTCGGCCTTCGTATTTCACATTTCGGACCCCACGG a V I M V I A V S C V K L L S A H N S T Q H T S R K H K V * S L G C L - b * S W S * L F P V * N C Y P L T I P H N I R A G S I K C K A W G A - c N H G H S C F L C E I V I R S Q F H T T Y E P E A * S V K P G V P - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d R L * P * L Q K R H S I T I R E C N W V V Y S G S A Y L T F G P T G - e Y D H D Y S N G T H F Q * G S V I G C L M R A P L M F H L A Q P A - f T I M T M A T E Q T F N N D A * L E V C C V L R F C L T Y L R P H - VspI PvuII VspI | | | TAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCG 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 ATTACTCACTCGATTGAGTGTAATTAACGCAACGCGAGTGACGGGCGAAAGGTCAGCCCTTTGGACAGCACGGTCGACGTAATTACTTAGCCGGTTGCGC a M S E L T H I N C V A L T A R F P V G K P V V P A A L M N R P T R - b * * V S * L T L I A L R S L P A F Q S G N L S C Q L H * * I G Q R A - c N E * A N S H * L R C A H C P L S S R E T C R A S C I N E S A N A - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d L S H A L E C * N R Q A * Q G S E L R S V Q R A L Q M L S D A L A - e * H T L * S V N I A N R E S G A K W D P F R D H W S C * H I P W R - f R I L S S V * M L Q T A S V A R K G T P F G T T G A A N I F R G V R - BsrBI | CGGGGAGAAGGCGGTTTGCGTATTGGGCGCTCTTTCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGGCGTTTCGGCTGGGGGGAGCGGTAT 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+-- 892 GCCCCTCTTCCGCCAAACGCATAACCCGCGAGAAAGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCCGCAAAGCCGACCCCCCTCGCCATA a G E K A V C V L G A L S L P R S L T R C A R A F R L G G A V - b G R R R F A Y W A L F R F L A H * L A A L G R F G W G E R Y - c R G E G G L R I G R S F A S S L T D S L R S G V S A G G S G - 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+-- 892 d R P S P P K R I P R E K A E E S V S E S R E P T E A P P L P I - e A P L L R N A Y Q A S K R K R A * Q S A A S P R K P Q P S R Y - f P S F A T Q T N P A R E S G R E S V R Q A R A N R S P P A T - Enzymes that do cut: AceIII ApaI BamHI BbsI BclI BglI BpmI Bpu1102I BsaI BsaBI BspMI BsrBI BssSI BstXI DraI DrdII EagI EciI Eco57I EcoRI EcoRV FseI HgiEII HindIII KpnI NgoAIV NotI NsiI PinAI PstI PvuII RcaI SacI SmaI SpeI SphI SrfI VspI XhoI Enzymes that do not cut: AatII AflII AhdI AlwNI ApaBI ApaLI AscI AvrII Bce83I BcgI BfiI BglII BplI Bpu10I BsaXI BsbI BseRI BsgI BsiWI BsmI BsmBI BspEI BspGI BspLU11I BsrDI BsrGI BssHII Bst1107I BstEII Bsu36I ClaI DraIII DrdI EarI Eco47III EcoNI FspI HpaI MfeI MluI MscI NarI NcoI NdeI NheI NruI NspV PacI Pfl1108I PflMI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SanDI SapI ScaI SexAI SfiI SgfI SgrAI SnaBI Sse8387I Sse8647I SspI StuI SwaI Tth111I XbaI XcmI XmnI