(Linear) MAP of: ./Fgr-S_1_B02_T7.seq check: 3344 from: 1 to: 905 Fgr-S_1_B02_T7 905 28 649 ABI Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * July 30, 2001 22:34 .. XhoI EagI EcoRV SphI| NotI BsaBI | EcoRI EarI SanDI NsiI ||BsrBI| BstXI | | PstI|Bpu1102I EagI SapI | | || || | | | || | | | CGGATTGAATTGGGACCCTCTANATGCATGCTCGAGCGGCCGCCAATGTGATGGATATCTGCAGAATTCGGCTTAGCGTGGTCGCGGCCGAGGTGAAGAG 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 GCCTAACTTAACCCTGGGAGATNTACGTACGAGCTCGCCGGCGGTTACACTACCTATAGACGTCTTAAGCCGAATCGCACCAGCGCCGGCTCCACTTCTC a R I E L G P S ? C M L E R P P M * W I S A E F G L A W S R P R * R A - b G L N W D P L ? A C S S G R Q C D G Y L Q N S A * R G R G R G E E - c D * I G T L ? M H A R A A A N V M D I C R I R L S V V A A E V K S - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d I S N P G E ? H M S S R G G I H H I D A S N P K A H D R G L H L - e S Q I P V R * ? C A R A A A L T I S I Q L I R S L T T A A S T F L - f P N F Q S G R ? A H E L P R W H S P Y R C F E A * R P R P R P S S - StuI BglI |EcoNI | | | CCGGTGAAGAAGGAGGCTGAATCCCAGATTGCCAGGAAGGCCTAGAGAAGGAAGCTCTACATAACCTATCTGCCTATTCGTTAGAGCAGTTTGTAGCATC 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 GGCCACTTCTTCCTCCGACTTAGGGTCTAACGGTCCTTCCGGATCTCTTCCTTCGAGATGTATTGGATAGACGGATAAGCAATCTCGTCAAACATCGTAG a G E E G G * I P D C Q E G L E K E A L H N L S A Y S L E Q F V A S - b P V K K E A E S Q I A R K A * R R K L Y I T Y L P I R * S S L * H L - c R * R R R L N P R L P G R P R E G S S T * P I C L F V R A V C S I - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d A P S S P P Q I G S Q W S P R S F S A R C L R D A * E N S C N T A D - e R H L L L S F G L N G P L G L S P L E V Y G I Q R N T L A T Q L M - f G T F F S A S D W I A L F A * L L F S * M V * R G I R * L L K Y C - NheI BsmBI | | TGGTCTGGCTGAGTCGAAATTTTTGGCCTGTTATCTGTCGTCTAAATACTGCTAGCTCATACTTTGTGAAACCTTTTGATGATCGTCTCATGTACCTGCC 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 ACCAGACCGACTCAGCTTTAAAAACCGGACAATAGACAGCAGATTTATGACGATCGAGTATGAAACACTTTGGAAAACTACTAGCAGAGTACATGGACGG a G L A E S K F L A C Y L S S K Y C * L I L C E T F * * S S H V P A - b V W L S R N F W P V I C R L N T A S S Y F V K P F D D R L M Y L P - c W S G * V E I F G L L S V V * I L L A H T L * N L L M I V S C T C P - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d P R A S D F N K A Q * R D D L Y Q * S M S Q S V K Q H D D * T G A - e Q D P Q T S I K P R N D T T * I S S A * V K H F R K I I T E H V Q - f R T Q S L R F K Q G T I Q R R F V A L E Y K T F G K S S R R M Y R G - EagI NotI BglI| BspMI|| SmaI ||| HindIII SrfI |||BsrBI EcoRI BstXI EagI SpeI BamHI SacI KpnI | | ||| | | | | | | | | | CGGGCGGCCGCTCGAAGCCGAATTCCAGCACACTGGCGGCCGTTACTAGTGGATCCGAGCTCGGTACCAAGCTTGGCGTAATCATGGTCATAGCTGTTTC 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 GCCCGCCGGCGAGCTTCGGCTTAAGGTCGTGTGACCGCCGGCAATGATCACCTAGGCTCGAGCCATGGTTCGAACCGCATTAGTACCAGTATCGACAAAG a R A A A R S R I P A H W R P L L V D P S S V P S L A * S W S * L F P - b G R P L E A E F Q H T G G R Y * W I R A R Y Q A W R N H G H S C F - c G G R S K P N S S T L A A V T S G S E L G T K L G V I M V I A V S - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d R A A A R L R I G A C Q R G N S T S G L E T G L K A Y D H D Y S N - e G P P R E F G F E L V S A A T V L P D S S P V L S P T I M T M A T E - f P R G S S A S N W C V P P R * * H I R A R Y W A Q R L * P * L Q K - BsrBI VspI | | CTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATT 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 GACACACTTTAACAATAGGCGAGTGTTAAGGTGTGTTGTATGCTCGGCCTTCGTATTTCACATTTCGGACCCCACGGATTACTCACTCGATTGAGTGTAA a V * N C Y P L T I P H N I R A G S I K C K A W G A * * V S * L T L - b L C E I V I R S Q F H T T Y E P E A * S V K P G V P N E * A N S H * - c C V K L L S A H N S T Q H T S R K H K V * S L G C L M S E L T H I - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d G T H F Q * G S V I G C L M R A P L M F H L A Q P A * H T L * S V N - e Q T F N N D A * L E V C C V L R F C L T Y L R P H R I L S S V * M - f R H S I T I R E C N W V V Y S G S A Y L T F G P T G L S H A L E C - PvuII VspI BsaXI | | | AATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATT 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 TTAACGCAACGCGAGTGACGGGCGAAAGGTCAGCCCTTTGGACAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCCCTCTCCGCCAAACGCATAA a I A L R S L P A F Q S G N L S C Q L H * * I G Q R A G R G G L R I - b L R C A H C P L S S R E T C R A S C I N E S A N A R G E A V C V L - c N C V A L T A R F P V G K P V V P A A L M N R P T R G E R R F A Y W - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d I A N R E S G A K W D P F R D H W S C * H I P W R A P L P P K R I - e L Q T A S V A R K G T P F G T T G A A N I F R G V R P S L R N A Y - f * N R Q A * Q G S E L R S V Q R A L Q M L S D A L A R P S A T Q T N - EarI Bst1107I SapI BsrBI AceIII | | | | | GGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCCGGTATACGGTTATCCA 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 CCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGGCCATATGCCAATAGGT a G R S S A S S L T D S L R S V V R L R R A V S A H S K A G I R L S T - b G A L P L P R S L T R C A R S F G C G E R Y Q L T Q R P V Y G Y P - c A L F R F L A H * L A A L G R S A A A S G I S S L K G R Y T V I H - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d P R E E A E E S V S E S R E T T R S R R A T D A * E F A P I R N D - e Q A S K R K R A * Q S A A S P R E A A A L P I L E S L P R Y V T I W - f P A R G S G R E S V R Q A R D N P Q P S R Y * S V * L G T Y P * G - BspLU11I | CAGGATCAGGTGATGACGCACGAAAGAACATGTGAGCAAAAGGCCAGCANCAAGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGGTTTTACGATAGCT 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 GTCCTAGTCCACTACTGCGTGCTTTCTTGTACACTCGTTTTCCGGTCGTNGTTCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCCAAAATGCTATCGA a G S G D D A R K N M * A K G Q ? Q A R N R K K A A L L A V L R * L - b Q D Q V M T H E R T C E Q K A S ? K P G T V K R P R C W R F Y D S F - c R I R * * R T K E H V S K R P A ? S Q E P * K G R V A G G F T I A - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d V P D P S S A R F F M H A F P W C ? A L F R L F A A N S A T K R Y S - e L I L H H R V F S C T L L L G A ? L W S G Y F P R T A P P K V I A - f C S * T I V C S L V H S C F A L ? L G P V T F L G R Q Q R N * S L - BssSI Bpu10I SacI | | | | TCGCNCCCCTGACGAGCATACAAAAATCGACGCTCAGGTTAGAGTGGCGAAACCCCACGGGACTATTAAGATACCAGCCCGTGCCCCCTGGAGCTCCCTC 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 AGCGNGGGGACTGCTCGTATGTTTTTAGCTGCGAGTCCAATCTCACCGCTTTGGGGTGCCCTGATAATTCTATGGTCGGGCACGGGGGACCTCGAGGGAG a R ? P D E H T K I D A Q V R V A K P H G T I K I P A R A P W S S L - b A P L T S I Q K S T L R L E W R N P T G L L R Y Q P V P P G A P S - c S ? P * R A Y K N R R S G * S G E T P R D Y * D T S P C P L E L P R - 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 d R ? G S S C V F I S A * T L T A F G W P V I L I G A R A G Q L E R - e E ? G Q R A Y L F R R E P * L P S V G R S * * S V L G H G R S S G - f K A G R V L M C F D V S L N S H R F G V P S N L Y W G T G G P A G E - GTGCC 901 ----- 905 CACGG a V - b C - c A - 901 ----- 905 d T G - e R A - f H - Enzymes that do cut: AceIII BamHI BglI Bpu10I Bpu1102I BsaBI BsaXI BsmBI BspLU11I BspMI BsrBI BssSI Bst1107I BstXI EagI EarI EcoNI EcoRI EcoRV HindIII KpnI NheI NotI NsiI PstI PvuII SacI SanDI SapI SmaI SpeI SphI SrfI StuI VspI XhoI Enzymes that do not cut: AatII AflII AhdI AlwNI ApaI ApaBI ApaLI AscI AvrII BbsI Bce83I BcgI BclI BfiI BglII BplI BpmI BsaI BsbI BseRI BsgI BsiWI BsmI BspEI BspGI BsrDI BsrGI BssHII BstEII Bsu36I ClaI DraI DraIII DrdI DrdII EciI Eco47III Eco57I FseI FspI HgiEII HpaI MfeI MluI MscI NarI NcoI NdeI NgoAIV NruI NspV PacI Pfl1108I PflMI PinAI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SexAI SfiI SgfI SgrAI SnaBI Sse8387I Sse8647I SspI SwaI Tth111I XbaI XcmI XmnI