(Linear) MAP of: ./Fgr-S_1_B04_T7.seq check: 4943 from: 1 to: 903 Fgr-S_1_B04_T7 903 31 632 ABI Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * July 30, 2001 22:34 .. EagI EcoRV XhoI NotI BsaBI | EcoRI NspV SanDI SphI|BsrBI| BstXI | | PstI|Bpu1102I EagI HindIII | | || || | | | || | | | GGGATTCGAATTGGGACCCCTCTANATGGCATGCTCGAGCGGCCGCCAGTGTGATGGATATCTGCAGAATTCGGCTTAGCGTGGTCGCGGCCGAGGTAAG 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 CCCTAAGCTTAACCCTGGGGAGATNTACCGTACGAGCTCGCCGGCGGTCACACTACCTATAGACGTCTTAAGCCGAATCGCACCAGCGCCGGCTCCATTC a G I R I G T P L ? G M L E R P P V * W I S A E F G L A W S R P R * A - b G F E L G P L ? M A C S S G R Q C D G Y L Q N S A * R G R G R G K - c D S N W D P S ? W H A R A A A S V M D I C R I R L S V V A A E V S - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d P N S N P G R * ? A H E L P R W H S P Y R C F E A * R P R P R P L - e I R I P V G R ? P M S S R G G T H H I D A S N P K A H D R G L Y - f S E F Q S G E ? H C A R A A A L T I S I Q L I R S L T T A A S T L - ApaBI | CTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGTAATTGCGATACTCTCTGGGAACTATCATTGTTCGGCAAGTTATGCTGCTCGCAGCCGATAACTACATG 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 GAAAAAAAAAAAAAAAAAAAAAAAAAAACTCATTAACGCTATGAGAGACCCTTGATAGTAACAAGCCGTTCAATACGACGAGCGTCGGCTATTGATGTAC a F F F F F F F F L S N C D T L W E L S L F G K L C C S Q P I T T C - b L F F F F F F F F * V I A I L S G N Y H C S A S Y A A R S R * L H A - c F F F F F F F F F E * L R Y S L G T I I V R Q V M L L A A D N Y M - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d S K K K K K K K K Q T I A I S E P F * * Q E A L * A A R L R Y S C - e A K K K K K K K K K L L Q S V R Q S S D N N P L N H Q E C G I V V H - f K K K K K K K K K S Y N R Y E R P V I M T R C T I S S A A S L * M - BsrDI BsaBI MscI| | || CAGTAAGGTACAAAGTCTGATACACAGTGCTGAAACGAAGATAAGTATCAGAGCTTGATATTGCTAGGGAAGTATCACAGGTTCAGAGAGCAATGGCCAC 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 GTCATTCCATGTTTCAGACTATGTGTCACGACTTTGCTTCTATTCATAGTCTCGAACTATAACGATCCCTTCATAGTGTCCAAGTCTCTCGTTACCGGTG a S K V Q S L I H S A E T K I S I R A * Y C * G S I T G S E S N G H - b V R Y K V * Y T V L K R R * V S E L D I A R E V S Q V Q R A M A T - c Q * G T K S D T Q C * N E D K Y Q S L I L L G K Y H R F R E Q W P H - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d A T L Y L T Q Y V T S F R L Y T D S S S I A L S T D C T * L A I A V - e L L T C L R I C L A S V F I L I L A Q Y Q * P L I V P E S L L P W - f C Y P V F D S V C H Q F S S L Y * L K I N S P F Y * L N L S C H G - BsbI AceIII | EarI | | PvuII AlwNI SapI | | AceIII | DraIII | XmnI BpmI| | | Bpu10I | | PstI | | | || | | | | | | ACAGTGCCTGATCGAAGTAGTTCAGCTCTTCTGAGTGTTGCCTCCCTCCAGCTCTTTCCTCAGCTGCAGTGAGAAATCATCGTTCACGTCGTCATCATCC 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 TGTCACGGACTAGCTTCATCAAGTCGAGAAGACTCACAACGGAGGGAGGTCGAGAAAGGAGTCGACGTCACTCTTTAGTAGCAAGTGCAGCAGTAGTAGG a T V P D R S S S A L L S V A S L Q L F P Q L Q * E I I V H V V I I P - b Q C L I E V V Q L F * V L P P S S S F L S C S E K S S F T S S S S - c S A * S K * F S S S E C C L P P A L S S A A V R N H R S R R H H P - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d C H R I S T T * S K Q T N G G E L E K R L Q L S F D D N V D D D D - e V T G S R L L E A R R L T A E R W S K G * S C H S I M T * T T M M - f C L A Q D F Y N L E E S H Q R G G A R E E A A T L F * R E R R * * G - EagI NotI BglI| BspMI|| SmaI ||| HindIII BfiI BsrDI SrfI |||BsrBI EcoRI BstXI SpeI BamHI SacI KpnI | | | | ||| | | | | | | | | CAGTCATCCTCCCATTGCACCTGCCCGGGCGGCCGCTCGAAAGCCGAATTCCAGCACACTGGCGGGCCGTTACTAGTGGATCCGAGCTCGGTACCAAGCT 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 GTCAGTAGGAGGGTAACGTGGACGGGCCCGCCGGCGAGCTTTCGGCTTAAGGTCGTGTGACCGCCCGGCAATGATCACCTAGGCTCGAGCCATGGTTCGA a V I L P L H L P G R P L E S R I P A H W R A V T S G S E L G T K L - b Q S S S H C T C P G G R S K A E F Q H T G G P L L V D P S S V P S L - c S H P P I A P A R A A A R K P N S S T L A G R Y * W I R A R Y Q A - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d W D D E W Q V Q G P P R E F A S N W C V P P G N S T S G L E T G L - e G T M R G N C R G P R G S S L R I G A C Q R A T V L P D S S P V L S - f L * G G M A G A R A A A R F G F E L V S A P R * * H I R A R Y W A - BsrBI | TGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGG 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 ACCGCATTAGTACCAGTATCGACAAAGGACACACTTTAACAATAGGCGAGTGTTAAGGTGTGTTGTATGCTCGGCCTTCGTATTTCACATTTCGGACCCC a G V I M V I A V S C V K L L S A H N S T Q H T S R K H K V * S L G - b A * S W S * L F P V * N C Y P L T I P H N I R A G S I K C K A W G - c W R N H G H S C F L C E I V I R S Q F H T T Y E P E A * S V K P G V - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d K A Y D H D Y S N G T H F Q * G S V I G C L M R A P L M F H L A Q P - e P T I M T M A T E Q T F N N D A * L E V C C V L R F C L T Y L R P - f Q R L * P * L Q K R H S I T I R E C N W V V Y S G S A Y L T F G P - VspI PvuII VspI | | | TGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAA 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 ACGGATTACTCACTCGATTGAGTGTAATTAACGCAACGCGAGTGACGGGCGAAAGGTCAGCCCTTTGGACAGCACGGTCGACGTAATTACTTAGCCGGTT a C L M S E L T H I N C V A L T A R F P V G K P V V P A A L M N R P T - b A * * V S * L T L I A L R S L P A F Q S G N L S C Q L H * * I G Q - c P N E * A N S H * L R C A H C P L S S R E T C R A S C I N E S A N - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d A * H T L * S V N I A N R E S G A K W D P F R D H W S C * H I P W - e H R I L S S V * M L Q T A S V A R K G T P F G T T G A A N I F R G - f T G L S H A L E C * N R Q A * Q G S E L R S V Q R A L Q M L S D A L - EarI SapI | CGCGCGGGGAGAAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGNCGTTCGGCTGCGGCAAGCGGTATCAGCT 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 GCGCGCCCCTCTTCCGCCAAACGCATAACCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCNGCAAGCCGACGCCGTTCGCCATAGTCGA a R G E K A V C V L G A L P L P R S L T R C A R ? F G C G K R Y Q L - b R A G R R R F A Y W A L F R F L A H * L A A L G R S A A A S G I S S - c A R G E G G L R I G R S S A S S L T D S L R S ? V R L R Q A V S A - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d R A P L L R N A Y Q A S K R K R A * Q S A A S P R E A A A L P I L - e V R P S F A T Q T N P A R G S G R E S V R Q A R ? N P Q P L R Y * S - f A R P S P P K R I P R E E A E E S V S E S R E ? T R S R C A T D A - AceIII | CACTCAAAGGCCGTAATACGGTATCCACAGATCAGGGGGATACGCAGGAAAGAACCTGTGACCAAAAGGCAGCAAAGGCCAGGAAACCGTAAAAGGGCCG 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 GTGAGTTTCCGGCATTATGCCATAGGTGTCTAGTCCCCCTATGCGTCCTTTCTTGGACACTGGTTTTCCGTCGTTTCCGGTCCTTTGGCATTTTCCCGGC a T Q R P * Y G I H R S G G Y A G K N L * P K G S K G Q E T V K G P - b L K G R N T V S T D Q G D T Q E R T C D Q K A A K A R K P * K G R - c H S K A V I R Y P Q I R G I R R K E P V T K R Q Q R P G N R K R A G - 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 d E S L P R L V T D V S * P S V C S L V Q S W F A A F A L F G Y F P R - e V * L G Y Y P I W L D P P Y A P F F R H G F P L L P W S V T F P G - f * E F A T I R Y G C I L P I R L F S G T V L L C C L G P F R L L A - GTG 901 --- 903 CAC a V - b - c - 901 --- 903 d H - e T - f P - Enzymes that do cut: AceIII AlwNI ApaBI BamHI BfiI BglI BpmI Bpu10I Bpu1102I BsaBI BsbI BspMI BsrBI BsrDI BstXI DraIII EagI EarI EcoRI EcoRV HindIII KpnI MscI NotI NspV PstI PvuII SacI SanDI SapI SmaI SpeI SphI SrfI VspI XhoI XmnI Enzymes that do not cut: AatII AflII AhdI ApaI ApaLI AscI AvrII BbsI Bce83I BcgI BclI BglII BplI BsaI BsaXI BseRI BsgI BsiWI BsmI BsmBI BspEI BspGI BspLU11I BsrGI BssHII BssSI Bst1107I BstEII Bsu36I ClaI DraI DrdI DrdII EciI Eco47III Eco57I EcoNI FseI FspI HgiEII HpaI MfeI MluI NarI NcoI NdeI NgoAIV NheI NruI NsiI PacI Pfl1108I PflMI PinAI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SexAI SfiI SgfI SgrAI SnaBI Sse8387I Sse8647I SspI StuI SwaI Tth111I XbaI XcmI