(Linear) MAP of: ./Fgr-S_1_B05_T7.seq check: 2155 from: 1 to: 1031 Fgr-S_1_B05_T7 1031 171 314 ABI Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * July 30, 2001 22:34 .. BspLU11I BssSI | | GCGACTGAGAGCGCAGGCGAGGGGAGAGGACGGTGTNTATNNNGNAAGCGTATTCTATAATGAAGTCGTCTTACATGTCACGAGNCGGNCGCCACTGTGA 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 CGCTGACTCTCGCGTCCGCTCCCCTCTCCTGCCACANATANNNCNTTCGCATAAGATATTACTTCAGCAGAATGTACAGTGCTCNGCCNGCGGTGACACT a A T E S A G E G R G R C ? ? ? K R I L * * S R L T C H E ? ? A T V I - b R L R A Q A R G E D G V Y ? ? S V F Y N E V V L H V T ? R ? P L * - c D * E R R R G E R T V ? ? ? ? A Y S I M K S S Y M S R ? G R H C D - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d V S L A P S P L P R H ? ? ? ? R I R Y H L R R V H * S ? ? A V T - e S Q S R L R P S L V T ? I ? ? A Y E I I F D D * M D R ? P R W Q S - f R S L A C A L P S S P T ? ? ? L T N * L S T T K C T V L R ? G S H - EcoRV EcoRI BsaBI | PstI| EagI ApaI BsaXI BsmBI | | || | | | | TAGATATCTGCAGAATTCGGCTTTTAATCGGCCGCCCCGACAGGTACAAACAAATTGGGGCCCGAAGATTCTACAAAGATTGAATACTTCGTCTCCTGGA 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 ATCTATAGACGTCTTAAGCCGAAAATTAGCCGGCGGGGCTGTCCATGTTTGTTTAACCCCGGGCTTCTAAGATGTTTCTAACTTATGAAGCAGAGGACCT a D I C R I R L L I G R P D R Y K Q I G A R R F Y K D * I L R L L E - b * I S A E F G F * S A A P T G T N K L G P E D S T K I E Y F V S W N - c R Y L Q N S A F N R P P R Q V Q T N W G P K I L Q R L N T S S P G - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d I S I Q L I R S K I P R G S L Y L C I P A R L N * L S Q I S R R R S - e L Y R C F E A K L R G G R C T C V F Q P G F I R C L N F V E D G P - f Y I D A S N P K * D A A G V P V F L N P G S S E V F I S Y K T E Q - NcoI DrdII MscI| BsmI | || | ACCAATTTATAAGTCGTCGAATGGGCCACTGGCCATGGAATGCTCAGTGATAAAATTCTTAATCTTTTGCAGGGCCTCCACAGCAATATCAGTTGGGGTT 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 TGGTTAAATATTCAGCAGCTTACCCGGTGACCGGTACCTTACGAGTCACTATTTTAAGAATTAGAAAACGTCCCGGAGGTGTCGTTATAGTCAACCCCAA a P I Y K S S N G P L A M E C S V I K F L I F C R A S T A I S V G V - b Q F I S R R M G H W P W N A Q * * N S * S F A G P P Q Q Y Q L G L - c T N L * V V E W A T G H G M L S D K I L N L L Q G L H S N I S W G * - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d G I * L D D F P G S A M S H E T I F N K I K Q L A E V A I D T P T - e V L K Y T T S H A V P W P I S L S L I R L R K C P R W L L I L Q P - f F W N I L R R I P W Q G H F A * H Y F E * D K A P G G C C Y * N P N - EarI RcaI SapI Bpu1102I | | | AGCTGAGAGACATCATCATGACCCTTTTTAGCTGCAAGCTCTTCAAAAGAAACCCTCACATCGGCGTATGCGTAAGCATCCCCTCTTTGCTCAGCGAGCA 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 TCGACTCTCTGTAGTAGTACTGGGAAAAATCGACGTTCGAGAAGTTTTCTTTGGGAGTGTAGCCGCATACGCATTCGTAGGGGAGAAACGAGTCGCTCGT a S * E T S S * P F L A A S S S K E T L T S A Y A * A S P L C S A S T - b A E R H H H D P F * L Q A L Q K K P S H R R M R K H P L F A Q R A - c L R D I I M T L F S C K L F K R N P H I G V C V S I P S L L S E H - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d L Q S V D D H G K K A A L E E F S V R V D A Y A Y A D G R Q E A L - e * S L S M M M V R K L Q L S K L L F G * M P T H T L M G E K S L S C - f A S L C * * S G K * S C A R * F F G E C R R I R L C G R K A * R A - EagI HindIII EagI Bpu1102I EcoRI BstXI NotI SpeI BamHI SacI KpnI | | | | | | | | | | | CGCTGAGTTTCGTGAAAGCCTGACCTCGGCCGCGACCACGCTAAGCCGAATTCCAGCACACTGGCGGCCGCTACTAGTGGATCCGAGCTCGGTACCAAGC 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 GCGACTCAAAGCACTTTCGGACTGGAGCCGGCGCTGGTGCGATTCGGCTTAAGGTCGTGTGACCGCCGGCGATGATCACCTAGGCTCGAGCCATGGTTCG a L S F V K A * P R P R P R * A E F Q H T G G R Y * W I R A R Y Q A - b R * V S * K P D L G R D H A K P N S S T L A A A T S G S E L G T K L - c A E F R E S L T S A A T T L S R I P A H W R P L L V D P S S V P S - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d V S L K T F A Q G R G R G R * A S N W C V P P R * * H I R A R Y W A - e A S N R S L R V E A A V V S L R I G A C Q R G S S T S G L E T G L - f R Q T E H F G S R P R S W A L G F E L V S A A A V L P D S S P V L - BsrBI EcoRV | | | TTGGCGTAATCATGGTCATAGCTGGTCCCTGTGCGAAATTGATATCCGCTCACAATTCCACACAACATACGACCCGGAAGCATAAAGGGTAAAGCCTGGG 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 AACCGCATTAGTACCAGTATCGACCAGGGACACGCTTTAACTATAGGCGAGTGTTAAGGTGTGTTGTATGCTGGGCCTTCGTATTTCCCATTTCGGACCC a W R N H G H S W S L C E I D I R S Q F H T T Y D P E A * R V K P G - b G V I M V I A G P C A K L I S A H N S T Q H T T R K H K G * S L G - c L A * S W S * L V P V R N * Y P L T I P H N I R P G S I K G K A W G - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d Q R L * P * L Q D R H S I S I R E C N W V V Y S G S A Y L T F G P - e K A Y D H D Y S T G T R F Q Y G S V I G C L M R G P L M F P L A Q - f S P T I M T M A P G Q A F N I D A * L E V C C V V R F C L P Y L R P - BseRI PvuII | | GTGCCTAATGAATGAACTAACTCACATTAACTGCTATTGCTCCTCACTGGCCCGCTTTGCAGATCGGGAACAGCTGAACGTGCCAGGTTGCATTAAATGA 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 CACGGATTACTTACTTGATTGAGTGTAATTGACGATAACGAGGAGTGACCGGGCGAAACGTCTAGCCCTTGTCGACTTGCACGGTCCAACGTAATTTACT a V P N E * T N S H * L L L L L T G P L C R S G T A E R A R L H * M K - b C L M N E L T H I N C Y C S S L A R F A D R E Q L N V P G C I K * - c A * * M N * L T L T A I A P H W P A L Q I G N S * T C Q V A L N E - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d T G L S H V L E C * S S N S R V P G S Q L D P V A S R A L N C * I - e P A * H I F * S V N V A I A G * Q G A K C I P F L Q V H W T A N F S - f H R I F S S V * M L Q * Q E E S A R K A S R S C S F T G P Q M L H - MfeI BsbI | | AATCAAACAAAATGTCGGGTGAAAAGCCGATTATACAATATGGGGAAGGTCTTACCAATTGCATCAATAGATGAACTCCGCTAGGCGTAAAAACAACAAC 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 TTAGTTTGTTTTACAGCCCACTTTTCGGCTAATATGTTATACCCCTTCCAGAATGGTTAACGTAGTTATCTACTTGAGGCGATCCGCATTTTTGTTGTTG a S N K M S G E K P I I Q Y G E G L T N C I N R * T P L G V K T T T - b N Q T K C R V K S R L Y N M G K V L P I A S I D E L R * A * K Q Q H - c I K Q N V G * K A D Y T I W G R S Y Q L H Q * M N S A R R K N N N - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d F D F L I D P S F G I I C Y P S P R V L Q M L L H V G S P T F V V V - e I L C F T P H F A S * V I H P L D * W N C * Y I F E A L R L F L L - f F * V F H R T F L R N Y L I P F T K G I A D I S S S R * A Y F C C - ACTATCATAAAAAACCGTAATTACCCCAGACCTCATAAGAAGAAGAATACAGGAAATTTAACAGAAAAACGAGGGGAAAAACTACCAGGAAAGAAGAACT 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 TGATAGTATTTTTTGGCATTAATGGGGTCTGGAGTATTCTTCTTCTTATGTCCTTTAAATTGTCTTTTTGCTCCCCTTTTTGATGGTCCTTTCTTCTTGA a L S * K T V I T P D L I R R R I Q E I * Q K N E G K N Y Q E R R T - b Y H K K P * L P Q T S * E E E Y R K F N R K T R G K T T R K E E L - c T I I K N R N Y P R P H K K K N T G N L T E K R G E K L P G K K N L - 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 d S D Y F V T I V G S R M L L L I C S I * C F F S P F F * W S L L V - e V I M F F R L * G L G * L F F F V P F K V S F R P S F S G P F F F - f C * * L F G Y N G W V E Y S S S Y L F N L L F V L P F V V L F S S S - EarI BsaI BsaXI SapI | | | TATGTAAACCGAAATGAAGCCCCACTGAAAAGGTACAGGTTAAAACCCGTGTAANGAAGGAGACCATGTGATATATATAGAGAGTAATAAGAAGAGCCTA 901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1000 ATACATTTGGCTTTACTTCGGGGTGACTTTTCCATGTCCAATTTTGGGCACATTNCTTCCTCTGGTACACTATATATATCTCTCATTATTCTTCTCGGAT a Y V N R N E A P L K R Y R L K P V * ? R R P C D I Y R E * * E E P K - b M * T E M K P H * K G T G * N P C ? E G D H V I Y I E S N K K S L - c C K P K * S P T E K V Q V K T R V ? K E T M * Y I * R V I R R A * - 901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1000 d * T F R F S A G S F L Y L N F G T Y ? L L G H S I Y L S Y Y S S G - e K H L G F H L G V S F T C T L V R T ? F S V M H Y I Y L T I L L A * - f I Y V S I F G W Q F P V P * F G H L S P S W T I Y I S L L L F L R - AAGGAGTGATCCTGACCCCCTCTTAATATAC 1001 ---------+---------+---------+- 1031 TTCCTCACTAGGACTGGGGGAGAATTATATG a G V I L T P S * Y - b K E * S * P P L N I - c R S D P D P L L I Y - 1001 ---------+---------+---------+- 1031 d L P T I R V G E * Y V - e L L S G S G R K I Y - f F S H D Q G G R L I - Enzymes that do cut: ApaI BamHI Bpu1102I BsaI BsaBI BsaXI BsbI BseRI BsmI BsmBI BspLU11I BsrBI BssSI BstXI DrdII EagI EarI EcoRI EcoRV HindIII KpnI MfeI MscI NcoI NotI PstI PvuII RcaI SacI SapI SpeI Enzymes that do not cut: AatII AceIII AflII AhdI AlwNI ApaBI ApaLI AscI AvrII BbsI Bce83I BcgI BclI BfiI BglI BglII BplI BpmI Bpu10I BsgI BsiWI BspEI BspGI BspMI BsrDI BsrGI BssHII Bst1107I BstEII Bsu36I ClaI DraI DraIII DrdI EciI Eco47III Eco57I EcoNI FseI FspI HgiEII HpaI MluI NarI NdeI NgoAIV NheI NruI NsiI NspV PacI Pfl1108I PflMI PinAI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SanDI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SphI SrfI Sse8387I Sse8647I SspI StuI SwaI Tth111I VspI XbaI XcmI XhoI XmnI