(Linear) MAP of: ./Fgr-S_1_B09_T7.seq check: 6919 from: 1 to: 888 Fgr-S_1_B09_T7 888 26 720 ABI Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * July 30, 2001 22:34 .. EagI XhoI NotI EcoRV EcoRI ApaI SphI|BsrBI| BsaBI | PstI|Bpu1102I EagI | || || | | || | | AGGATTGAATTGGGCCCTCTATAGCATGCTCGAGCGGCCGCCGTGTGATGGATATCTGCAGAATTCGGCTTAGCGTGGTCGCGGCCGAGGTACTTTTTCA 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 TCCTAACTTAACCCGGGAGATATCGTACGAGCTCGCCGGCGGCACACTACCTATAGACGTCTTAAGCCGAATCGCACCAGCGCCGGCTCCATGAAAAAGT a R I E L G P L * H A R A A A V * W I S A E F G L A W S R P R Y F F S - b G L N W A L Y S M L E R P P C D G Y L Q N S A * R G R G R G T F S - c D * I G P S I A C S S G R R V M D I C R I R L S V V A A E V L F Q - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d P N F Q A R * L M S S R G G H S P Y R C F E A * R P R P R P V K E - e I S N P G R Y C A R A A A T H H I D A S N P K A H D R G L Y K K - f S Q I P G E I A H E L P R R T I S I Q L I R S L T T A A S T S K * - Eco57I | GCACAATATACAATGGTCAAAATATAAATTTTTCTGATAACCATGAACATTTTGACCTTATTCATTATGTATTCGATATACTTCAGTTTAGTTGAATTTC 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 CGTGTTATATGTTACCAGTTTTATATTTAAAAAGACTATTGGTACTTGTAAAACTGGAATAAGTAATACATAAGCTATATGAAGTCAAATCAACTTAAAG a T I Y N G Q N I N F S D N H E H F D L I H Y V F D I L Q F S * I S - b A Q Y T M V K I * I F L I T M N I L T L F I M Y S I Y F S L V E F L - c H N I Q W S K Y K F F * * P * T F * P Y S L C I R Y T S V * L N F - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d A C Y V I T L I Y I K R I V M F M K V K N M I Y E I Y K L K T S N - e L V I Y L P * F I F K E S L W S C K S R I * * T N S I S * N L Q I E - f C L I C H D F Y L N K Q Y G H V N Q G * E N H I R Y V E T * N F K - TTTATGAAGCCGATTATGAGCGCACTAATTTCCTCTGGCTTTTCTTCATTTATGAAATGCCCCACATCTTTCATTATCACCACGTCATCTAGTAATGGAA 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 AAATACTTCGGCTAATACTCGCGTGATTAAAGGAGACCGAAAAGAAGTAAATACTTTACGGGGTGTAGAAAGTAATAGTGGTGCAGTAGATCATTACCTT a L * S R L * A H * F P L A F L H L * N A P H L S L S P R H L V M E - b Y E A D Y E R T N F L W L F F I Y E M P H I F H Y H H V I * * W N - c F M K P I M S A L I S S G F S S F M K C P T S F I I T T S S S N G T - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d R * S A S * S R V L K R Q S K K M * S I G W M K * * * W T M * Y H F - e K H L R N H A C * N G R A K R * K H F A G C R E N D G R * R T I S - f K I F G I I L A S I E E P K E E N I F H G V D K M I V V D D L L P - EagI NotI BglI| BspMI|| SmaI ||| BsaI Bce83I SspI BglII SrfI ||| | | | | | ||| CAAACTTCTTGAGACCACCTTTGTTAATGAAAACTTTTGCCCCTGCAATATTGTATGTTAGATCTAAGTTGCCAACTGTGAACTTCACCTGCCCGGGCGG 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 GTTTGAAGAACTCTGGTGGAAACAATTACTTTTGAAAACGGGGACGTTATAACATACAATCTAGATTCAACGGTTGACACTTGAAGTGGACGGGCCCGCC a Q T S * D H L C * * K L L P L Q Y C M L D L S C Q L * T S P A R A A - b K L L E T T F V N E N F C P C N I V C * I * V A N C E L H L P G R - c N F L R P P L L M K T F A P A I L Y V R S K L P T V N F T C P G G - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d L S R S V V K T L S F K Q G Q L I T H * I * T A L Q S S * R G P R - e C V E Q S W R Q * H F S K G R C Y Q I N S R L Q W S H V E G A R A - f V F K K L G G K N I F V K A G A I N Y T L D L N G V T F K V Q G P P - HindIII BsrBI EcoRI BstXI EagI SpeI BamHI SacI KpnI | | | | | | | | | | CCGCTCGAAAGCCGAATTCCAGCACACTGGCGGCCGTTACTAGTGGATCCGAGCTCGGTACCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGT 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 GGCGAGCTTTCGGCTTAAGGTCGTGTGACCGCCGGCAATGATCACCTAGGCTCGAGCCATGGTTCGAACCGCATTAGTACCAGTATCGACAAAGGACACA a A R K P N S S T L A A V T S G S E L G T K L G V I M V I A V S C V - b P L E S R I P A H W R P L L V D P S S V P S L A * S W S * L F P V * - c R S K A E F Q H T G G R Y * W I R A R Y Q A W R N H G H S C F L C - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d G S S L R I G A C Q R G N S T S G L E T G L K A Y D H D Y S N G T - e A A R F G F E L V S A A T V L P D S S P V L S P T I M T M A T E Q T - f R E F A S N W C V P P R * * H I R A R Y W A Q R L * P * L Q K R H - BsrBI VspI | | GAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGC 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 CTTTAACAATAGGCGAGTGTTAAGGTGTGTTGTATGCTCGGCCTTCGTATTTCACATTTCGGACCCCACGGATTACTCACTCGATTGAGTGTAATTAACG a K L L S A H N S T Q H T S R K H K V * S L G C L M S E L T H I N C - b N C Y P L T I P H N I R A G S I K C K A W G A * * V S * L T L I A - c E I V I R S Q F H T T Y E P E A * S V K P G V P N E * A N S H * L R - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d H F Q * G S V I G C L M R A P L M F H L A Q P A * H T L * S V N I A - e F N N D A * L E V C C V L R F C L T Y L R P H R I L S S V * M L Q - f S I T I R E C N W V V Y S G S A Y L T F G P T G L S H A L E C * N - PvuII VspI BsaXI | | | GTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGC 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 CAACGCGAGTGACGGGCGAAAGGTCAGCCCTTTGGACAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCCCTCTCCGCCAAACGCATAACCCGCG a V A L T A R F P V G K P V V P A A L M N R P T R G E R R F A Y W A L - b L R S L P A F Q S G N L S C Q L H * * I G Q R A G R G G L R I G R - c C A H C P L S S R E T C R A S C I N E S A N A R G E A V C V L G A - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d N R E S G A K W D P F R D H W S C * H I P W R A P L P P K R I P R - e T A S V A R K G T P F G T T G A A N I F R G V R P S L R N A Y Q A - f R Q A * Q G S E L R S V Q R A L Q M L S D A L A R P S A T Q T N P A - EarI Bst1107I SapI BsrBI AceIII | | | | | TCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCTGCGAGCGGTATCAGCTCACTCAAAGGACGGTATACGGTTATCCACAGAAT 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 AGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGACGCTCGCCATAGTCGAGTGAGTTTCCTGCCATATGCCAATAGGTGTCTTA a F R F L A H * L A A L G R S A A A S G I S S L K G R Y T V I H R I - b S S A S S L T D S L R S V V R L L R A V S A H S K D G I R L S T E S - c L P L P R S L T R C A R S F G C C E R Y Q L T Q R T V Y G Y P Q N - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d E E A E E S V S E S R E T T R S S R A T D A * E F S P I R N D V S - e S K R K R A * Q S A A S P R E A A A L P I L E S L P R Y V T I W L I - f R G S G R E S V R Q A R D N P Q Q S R Y * S V * L V T Y P * G C F - BspLU11I | CAGGTGATGACGCACGAAAGAACATGTGAGCAAAGGCCAGCAAATGCCAGNAACCGTAAAAGGCCGCGTTGCCTGGCGTTTTTCATAG 801 ---------+---------+---------+---------+---------+---------+---------+---------+-------- 888 GTCCACTACTGCGTGCTTTCTTGTACACTCGTTTCCGGTCGTTTACGGTCNTTGGCATTTTCCGGCGCAACGGACCGCAAAAAGTATC a R * * R T K E H V S K G Q Q M P ? T V K G R V A W R F S * - b G D D A R K N M * A K A S K C Q ? P * K A A L P G V F H - c Q V M T H E R T C E Q R P A N A ? N R K R P R C L A F F I - 801 ---------+---------+---------+---------+---------+---------+---------+---------+-------- 888 d D P S S A R F F M H A F A L L H W ? G Y F A A N G P T K * L - e L H H R V F S C T L L P W C I G ? V T F P R T A Q R K E Y - f * T I V C S L V H S C L G A F A L ? R L L G R Q R A N K M - Enzymes that do cut: AceIII ApaI BamHI Bce83I BglI BglII Bpu1102I BsaI BsaBI BsaXI BspLU11I BspMI BsrBI Bst1107I BstXI EagI EarI Eco57I EcoRI EcoRV HindIII KpnI NotI PstI PvuII SacI SapI SmaI SpeI SphI SrfI SspI VspI XhoI Enzymes that do not cut: AatII AflII AhdI AlwNI ApaBI ApaLI AscI AvrII BbsI BcgI BclI BfiI BplI BpmI Bpu10I BsbI BseRI BsgI BsiWI BsmI BsmBI BspEI BspGI BsrDI BsrGI BssHII BssSI BstEII Bsu36I ClaI DraI DraIII DrdI DrdII EciI Eco47III EcoNI FseI FspI HgiEII HpaI MfeI MluI MscI NarI NcoI NdeI NgoAIV NheI NruI NsiI NspV PacI Pfl1108I PflMI PinAI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SanDI ScaI SexAI SfiI SgfI SgrAI SnaBI Sse8387I Sse8647I StuI SwaI Tth111I XbaI XcmI XmnI