(Linear) MAP of: ./Fgr-S_1_B11_T7.seq check: 9535 from: 1 to: 932 Fgr-S_1_B11_T7 932 102 573 ABI Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * July 30, 2001 22:34 .. EciI EagI | | CGGGCGCGGGCGGGCGGGGCGGGCGAGGGCGGAGCNGTTTCTTTGNNNGNAGCNATGTTTAGGATCTCTGATTGCTGCTCGACGGCCGCCATGTGTAGGN 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 GCCCGCGCCCGCCCGCCCCGCCCGCTCCCGCCTCGNCAAAGAAACNNNCNTCGNTACAAATCCTAGAGACTAACGACGAGCTGCCGGCGGTACACATCCN a R A R A G G A G E G G A V S L ? ? A M F R I S D C C S T A A M C R ? - b G R G R A G R A R A E ? F L ? ? ? ? C L G S L I A A R R P P C V G - c G A G G R G G R G R S ? F F ? ? S ? V * D L * L L L D G R H V * ? - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d A R A P P A P S P P A T E K ? ? A ? N L I E S Q Q E V A A M H L - e P A P P R P P R P R L ? K K ? ? L ? T * S R Q N S S S P R W T Y ? - f P R P R A P R A L A S ? N R Q ? ? ? H K P D R I A A R R G G H T P - EcoRI PstI|Bpu1102I EagI Bst1107I || | | | AATCTGCAGAATTCGGCTTAGCGTGGTCGCGGCCGAGGTATCGTTAGTTTGTTACTAATTCTTTCTTTTTACCGAGTATACATGATTTTTTCCCCTTTCG 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 TTAGACGTCTTAAGCCGAATCGCACCAGCGCCGGCTCCATAGCAATCAAACAATGATTAAGAAAGAAAAATGGCTCATATGTACTAAAAAAGGGGAAAGC a S A E F G L A W S R P R Y R * F V T N S F F L P S I H D F F P F R - b N L Q N S A * R G R G R G I V S L L L I L S F Y R V Y M I F S P F D - c I C R I R L S V V A A E V S L V C Y * F F L F T E Y T * F F P L S - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d ? D A S N P K A H D R G L Y R * N T V L E K K K G L I C S K K G K R - e I Q L I R S L T T A A S T D N T Q * * N K R K V S Y V H N K G R E - f ? R C F E A * R P R P R P I T L K N S I R E K * R T Y M I K E G K - EagI NotI BglI| BspMI|| SmaI ||| SrfI |||BsrBI EcoRI BstXI EagI | ||| | | | | ATTGCTAGGGAGATACTACCGTGCCCCGGTATATGATGATTTTACGAGTACCTGCCCGGGCGGCCGCTCGAAAGCCGAATTCCAGCACACTGGCGGCCGT 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 TAACGATCCCTCTATGATGGCACGGGGCCATATACTACTAAAATGCTCATGGACGGGCCCGCCGGCGAGCTTTCGGCTTAAGGTCGTGTGACCGCCGGCA a L L G R Y Y R A P V Y D D F T S T C P G G R S K A E F Q H T G G R - b C * G D T T V P R Y M M I L R V P A R A A A R K P N S S T L A A V - c I A R E I L P C P G I * * F Y E Y L P G R P L E S R I P A H W R P L - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d N S P L Y * R A G T Y S S K V L V Q G P P R E F A S N W C V P P R - e I A L S I S G H G P I H H N * S Y R G P R G S S L R I G A C Q R G - f S Q * P S V V T G R Y I I I K R T G A R A A A R F G F E L V S A A T - HindIII SpeI BamHI SacI KpnI | BsrBI | | | | | | TACTAGTGGATCCGAGCTCGGTACCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACG 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 ATGATCACCTAGGCTCGAGCCATGGTTCGAACCGCATTAGTACCAGTATCGACAAAGGACACACTTTAACAATAGGCGAGTGTTAAGGTGTGTTGTATGC a Y * W I R A R Y Q A W R N H G H S C F L C E I V I R S Q F H T T Y E - b T S G S E L G T K L G V I M V I A V S C V K L L S A H N S T Q H T - c L V D P S S V P S L A * S W S * L F P V * N C Y P L T I P H N I R - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d * * H I R A R Y W A Q R L * P * L Q K R H S I T I R E C N W V V Y - e N S T S G L E T G L K A Y D H D Y S N G T H F Q * G S V I G C L M R - f V L P D S S P V L S P T I M T M A T E Q T F N N D A * L E V C C V - VspI | AGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTG 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 TCGGCCTTCGTATTTCACATTTCGGACCCCACGGATTACTCACTCGATTGAGTGTAATTAACGCAACGCGAGTGACGGGCGAAAGGTCAGCCCTTTGGAC a P E A * S V K P G V P N E * A N S H * L R C A H C P L S S R E T C - b S R K H K V * S L G C L M S E L T H I N C V A L T A R F P V G K P V - c A G S I K C K A W G A * * V S * L T L I A L R S L P A F Q S G N L - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d S G S A Y L T F G P T G L S H A L E C * N R Q A * Q G S E L R S V Q - e A P L M F H L A Q P A * H T L * S V N I A N R E S G A K W D P F R - f L R F C L T Y L R P H R I L S S V * M L Q T A S V A R K G T P F G - EarI PvuII VspI BsaXI SapI | | | | TCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGG 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 AGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCCCTCTCCGCCAAACGCATAACCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCC a R A S C I N E S A N A R G E A V C V L G A L P L P R S L T R C A R - b V P A A L M N R P T R G E R R F A Y W A L F R F L A H * L A A L G - c S C Q L H * * I G Q R A G R G G L R I G R S S A S S L T D S L R S V - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d R A L Q M L S D A L A R P S A T Q T N P A R G S G R E S V R Q A R - e D H W S C * H I P W R A P L P P K R I P R E E A E E S V S E S R E - f T T G A A N I F R G V R P S L R N A Y Q A S K R K R A * Q S A A S P - BsrBI AceIII BspLU11I | | | TCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCATGAAAGAACATGTGAGCAAAAGG 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 AGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTACTTTCTTGTACACTCGTTTTCC a S F G C G E R Y Q L T Q R R * Y G Y P Q N Q G I T H E R T C E Q K A - b R S A A A S G I S S L K G G N T V I H R I R G * R M K E H V S K R - c V R L R R A V S A H S K A V I R L S T E S G D N A * K N M * A K G - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d D N P Q P S R Y * S V * L R Y Y P * G C F * P I V C S L V H S C F - e T T R S R R A T D A * E F A T I R N D V S D P S L A H F F M H A F P - f R E A A A L P I L E S L P P L V T I W L I L P Y R M F S C T L L L - BsaXI EciI Bce83I DrdI | | | | CCAGCAAAAGGCCATGAACCGTACAAAGGCCGCGTTGCTGGCGTTTATACATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAG 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 GGTCGTTTTCCGGTACTTGGCATGTTTCCGGCGCAACGACCGCAAATATGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTC a S K R P * T V Q R P R C W R L Y I G S A P L T S I T K I D A Q V R - b P A K G H E P Y K G R V A G V Y T * A P P P * R A S Q K S T L K S E - c Q Q K A M N R T K A A L L A F I H R L R P P D E H H K N R R S S Q - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d A L L L G H V T C L G R Q Q R K Y M P E A G R V L M V F I S A * T L - e W C F A M F R V F A A N S A N I C L S R G G S S C * L F R R E L * - f G A F P W S G Y L P R T A P T * V Y A G G G Q R A D C F D V S L D - BssSI | AAGTGGCGAAACCCGACAGGACTATTAAGATACCAGGCGTNTACCCCCTGGAAAGCTCCCTCGTGCGCTCTACTGTTCCCGACCCTGCCGCTTACCGGGA 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 TTCACCGCTTTGGGCTGTCCTGATAATTCTATGGTCCGCANATGGGGGACCTTTCGAGGGAGCACGCGAGATGACAAGGGCTGGGACGGCGAATGGCCCT a S G E T R Q D Y * D T R R ? P P G K L P R A L Y C S R P C R L P G - b V A K P D R T I K I P G V Y P L E S S L V R S T V P D P A A Y R E - c K W R N P T G L L R Y Q A ? T P W K A P S C A L L F P T L P L T G N - 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 d L P S V R C S * * S V L R ? G G P F S G R A S * Q E R G Q R K G P - e F H R F G V P S N L Y W A ? V G Q F A G E H A R S N G V R G S V P - f S T A F G S L V I L I G P T ? G R S L E R T R E V T G S G A A * R S - DrdII EciI Bsu36I | | | ACCATGTCCGCCTTTTCTCCCTTAGGCAAAGC 901 ---------+---------+---------+-- 932 TGGTACAGGCGGAAAAGAGGGAATCCGTTTCG a T M S A F S P L G K - b P C P P F L P * A K - c H V R L F S L R Q S - 901 ---------+---------+---------+-- 932 d V M D A K E G K P L A - e F W T R R K E R L C L - f G H G G K R G * A F - Enzymes that do cut: AceIII BamHI Bce83I BglI Bpu1102I BsaXI BspLU11I BspMI BsrBI BssSI Bst1107I BstXI Bsu36I DrdI DrdII EagI EarI EciI EcoRI HindIII KpnI NotI PstI PvuII SacI SapI SmaI SpeI SrfI VspI Enzymes that do not cut: AatII AflII AhdI AlwNI ApaI ApaBI ApaLI AscI AvrII BbsI BcgI BclI BfiI BglII BplI BpmI Bpu10I BsaI BsaBI BsbI BseRI BsgI BsiWI BsmI BsmBI BspEI BspGI BsrDI BsrGI BssHII BstEII ClaI DraI DraIII Eco47III Eco57I EcoNI EcoRV FseI FspI HgiEII HpaI MfeI MluI MscI NarI NcoI NdeI NgoAIV NheI NruI NsiI NspV PacI Pfl1108I PflMI PinAI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SanDI ScaI SexAI SfiI SgfI SgrAI SnaBI SphI Sse8387I Sse8647I SspI StuI SwaI Tth111I XbaI XcmI XhoI XmnI