(Linear) MAP of: ./Fgr-S_1_B12_T7.seq check: 4564 from: 1 to: 889 Fgr-S_1_B12_T7 889 60 186 ABI Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * July 30, 2001 22:34 .. EagI NotI EcoRV BsrBI| BsaBI | EcoRI SanDI XhoI || BstXI | | PstI|Bpu1102I EagI | | || | | | || | | CATTGGNNGAAGGAATACCGATAAGGGACCCTCTAATAGCTGACTCGAGCGGCCGCCAATGTGATGGATATCTGCAGAATTCGGCTTAGCGTGGTCGCGG 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 GTAACCNNCTTCCTTATGGCTATTCCCTGGGAGATTATCGACTGAGCTCGCCGGCGGTTACACTACCTATAGACGTCTTAAGCCGAATCGCACCAGCGCC a H W ? K E Y R * G T L * * L T R A A A N V M D I C R I R L S V V A A - b I G ? R N T D K G P S N S * L E R P P M * W I S A E F G L A W S R - c L ? E G I P I R D P L I A D S S G R Q C D G Y L Q N S A * R G R G - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d N ? S P I G I L S G R I A S E L P R W H S P Y R C F E A * R P R P - e M P ? L F V S L P G E L L Q S S R G G I H H I D A S N P K A H D R - f Q ? F S Y R Y P V R * Y S V R A A A L T I S I Q L I R S L T T A - BseRI | CCGAGGTCAGCCGTGCTAATTCTAGTCGTTGGGATACGTCCTTGGAGGGGTTTCTTCCTTGTTAAATCCTTCTCCTCCATTGGTTTTTTCATACAGTGCA 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 GGCTCCAGTCGGCACGATTAAGATCAGCAACCCTATGCAGGAACCTCCCCAAAGAAGGAACAATTTAGGAAGAGGAGGTAACCAAAAAAGTATGTCACGT a E V S R A N S S R W D T S L E G F L P C * I L L L H W F F H T V H - b P R S A V L I L V V G I R P W R G F F L V K S F S S I G F F I Q C M - c R G Q P C * F * S L G Y V L G G V S S L L N P S P P L V F S Y S A - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d R P * G H * N * D N P Y T R P P T E E K N F G E G G N T K E Y L A - e G L D A T S I R T T P I R G Q L P K K R T L D K E E M P K K M C H - f A S T L R A L E L R Q S V D K S P N R G Q * I R R R W Q N K * V T C - NsiI SphI | BclI EcoRV | | | | TGCATCACTGTAAGGATGATCATATATGATAGGAACAGTTCATGTGTAGGAGTTATAGGGGGATGNTGATGGTGTAGTGATGCGGGGATGTATGGATATC 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 ACGTAGTGACATTCCTACTAGTATATACTATCCTTGTCAAGTACACATCCTCAATATCCCCCTACNACTACCACATCACTACGCCCCTACATACCTATAG a A S L * G * S Y M I G T V H V * E L * G D ? D G V V M R G C M D I - b H H C K D D H I * * E Q F M C R S Y R G M ? M V * * C G D V W I S - c C I T V R M I I Y D R N S S C V G V I G G ? * W C S D A G M Y G Y L - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d H M V T L I I M Y S L F L E H T P T I P P H ? H H L S A P I Y P Y - e M C * Q L S S * I H Y S C N M H L L * L P I ? I T Y H H P S T H I D - f A D S Y P H D Y I I P V T * T Y S N Y P S ? S P T T I R P H I S I - BsrBI EcoRI BcgI EagI | | | | TCATATTGCTTCTAGTTAATGTGAACACGAATGTTAGCAGAACCTACCCGGACTGGCCGCTCGAAAGCCGAATTCCAGCCCACTGACGGCCGATTCTAGT 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 AGTATAACGAAGATCAATTACACTTGTGCTTACAATCGTCTTGGATGGGCCTGACCGGCGAGCTTTCGGCTTAAGGTCGGGTGACTGCCGGCTAAGATCA a S Y C F * L M * T R M L A E P T R T G R S K A E F Q P T D G R F * C - b H I A S S * C E H E C * Q N L P G L A A R K P N S S P L T A D S S - c I L L L V N V N T N V S R T Y P D W P L E S R I P A H * R P I L V - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d R M N S R T L T F V F T L L V * G S Q G S S L R I G A W Q R G I R T - e * I A E L * H S C S H * C F R G P S A A R F G F E L G S V A S E L - f E Y Q K * N I H V R I N A S G V R V P R E F A S N W G V S P R N * - HindIII KpnI | BcgI | | SacI | | | NcoI BsrBI | | | | | | GCATCCGAGCTCGGTACCAAGCTTGACCTAACCATGGACATAGCTGTTTCCTGTCTGAAACTGATCTCCGCTCACAATGACACACAACATACGATCCAGA 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 CGTAGGCTCGAGCCATGGTTCGAACTGGATTGGTACCTGTATCGACAAAGGACAGACTTTGACTAGAGGCGAGTGTTACTGTGTGTTGTATGCTAGGTCT a I R A R Y Q A * P N H G H S C F L S E T D L R S Q * H T T Y D P E - b A S E L G T K L D L T M D I A V S C L K L I S A H N D T Q H T I Q K - c H P S S V P S L T * P W T * L F P V * N * S P L T M T H N I R S R - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d C G L E T G L K V * G H V Y S N G T Q F Q D G S V I V C L M R D L - e A D S S P V L S S R V M S M A T E Q R F S I E A * L S V C C V I W - f H M R A R Y W A Q G L W P C L Q K R D S V S R R E C H C V V Y S G S - VspI Eco47III | | AGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAATGAGCTAACTCACATTAATTACTTAGCGCTCTCTGTCCGCTTTCCAGTCGGTAAACCTGTCGTGCC 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 TCGTATTTCACATTTCGGACCCCACGGATTACTTACTCGATTGAGTGTAATTAATGAATCGCGAGAGACAGGCGAAAGGTCAGCCATTTGGACAGCACGG a A * S V K P G V P N E * A N S H * L L S A L C P L S S R * T C R A - b H K V * S L G C L M N E L T H I N Y L A L S V R F P V G K P V V P - c S I K C K A W G A * * M S * L T L I T * R S L S A F Q S V N L S C Q - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d L M F H L A Q P A * H I L * S V N I V * R E R D A K W D T F R D H - e F C L T Y L R P H R I F S S V * M L * K A S E T R K G T P L G T T G - f A Y L T F G P T G L S H A L E C * N S L A R Q G S E L R Y V Q R A - VspI BsrBI BsgI | | | AACTGCATTAATGAATCGGCCCACGCGCGTAGATGAGCGGTTAGCGTATTGGGCACAGTTCCGCTTCCTCTCTCACTGACTCGCTGCACCTCGTCATTCG 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 TTGACGTAATTACTTAGCCGGGTGCGCGCATCTACTCGCCAATCGCATAACCCGTGTCAAGGCGAAGGAGAGAGTGACTGAGCGACGTGGAGCAGTAAGC a N C I N E S A H A R R * A V S V L G T V P L P L S L T R C T S S F G - b T A L M N R P T R V D E R L A Y W A Q F R F L S H * L A A P R H S - c L H * * I G P R A * M S G * R I G H S S A S S L T D S L H L V I R - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d W S C * H I P G R A Y I L P * R I P C L E A E E R V S E S C R T M R - e V A N I F R G V R T S S R N A Y Q A C N R K R E * Q S A A G R * E - f L Q M L S D A W A R L H A T L T N P V T G S G R E S V R Q V E D N - BspLU11I BsaI PmlI Pfl1108I | BclI | | | | | GCTGCTGCAATCGGTATCATCTCACTCAGAGACCGTTATACGGTTATCCACAGGATCACGTGATGACGCTACGAAAAACATGTGATCACAAAGCCACCAA 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 CGACGACGTTAGCCATAGTAGAGTGAGTCTCTGGCAATATGCCAATAGGTGTCCTAGTGCACTACTGCGATGCTTTTTGTACACTAGTGTTTCGGTGGTT a C C N R Y H L T Q R P L Y G Y P Q D H V M T L R K T C D H K A T N - b A A A I G I I S L R D R Y T V I H R I T * * R Y E K H V I T K P P T - c L L Q S V S S H S E T V I R L S T G S R D D A T K N M * S Q S H Q - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d S S C D T D D * E S V T I R N D V P D R S S A V F F M H D C L W W - e A A A I P I M E S L S R * V T I W L I V H H R * S F C T I V F G G - f P Q Q L R Y * R V * L G N Y P * G C S * T I V S R F V H S * L A V L - RcaI | CATGCCAGCAACCGTTCATGGGCGGCTTGCTACCGTTAATAACTAGGCTTCGCCGCCCTGACCATCATGACAAAATCGACGATCAAATA 801 ---------+---------+---------+---------+---------+---------+---------+---------+--------- 889 GTACGGTCGTTGGCAAGTACCCGCCGAACGATGGCAATTATTGATCCGAAGCGGCGGGACTGGTAGTACTGTTTTAGCTGCTAGTTTAT a M P A T V H G R L A T V N N * A S P P * P S * Q N R R S N - b C Q Q P F M G G L L P L I T R L R R P D H H D K I D D Q I - c H A S N R S W A A C Y R * * L G F A A L T I M T K S T I K - 801 ---------+---------+---------+---------+---------+---------+---------+---------+--------- 889 d C A L L R E H A A Q * R * Y S P K A A R V M M V F D V I L Y - e V H W C G N M P P K S G N I V L S R R G S W * S L I S S * I - f M G A V T * P R S A V T L L * A E G G Q G D H C F R R D F - Enzymes that do cut: BcgI BclI Bpu1102I BsaI BsaBI BseRI BsgI BspLU11I BsrBI BstXI EagI Eco47III EcoRI EcoRV HindIII KpnI NcoI NotI NsiI Pfl1108I PmlI PstI RcaI SacI SanDI SphI VspI XhoI Enzymes that do not cut: AatII AceIII AflII AhdI AlwNI ApaI ApaBI ApaLI AscI AvrII BamHI BbsI Bce83I BfiI BglI BglII BplI BpmI Bpu10I BsaXI BsbI BsiWI BsmI BsmBI BspEI BspGI BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstEII Bsu36I ClaI DraI DraIII DrdI DrdII EarI EciI Eco57I EcoNI FseI FspI HgiEII HpaI MfeI MluI MscI NarI NdeI NgoAIV NheI NruI NspV PacI PflMI PinAI PmeI PshAI Psp1406I PvuI PvuII RsrII SacII SalI SapI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI Sse8387I Sse8647I SspI StuI SwaI Tth111I XbaI XcmI XmnI