(Linear) MAP of: ./Fgr-S_1_C04_T7.seq check: 4009 from: 1 to: 898 Fgr-S_1_C04_T7 898 43 584 ABI Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * July 30, 2001 22:34 .. BssHII XhoI | EcoRV SphI| | BsaBI | EcoRI SanDI NsiI || | BstXI | | PstI|Bpu1102I EagI | | || | | | | || | | CGGATTTGAATTGGGACCCTCTANATGCATGCTCGAGCGCGCCGCCAGTGTGATGGATATCTGCAGAATTCGGCTTAGCGTGGTCGCGGCCGAGGTCCAT 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 GCCTAAACTTAACCCTGGGAGATNTACGTACGAGCTCGCGCGGCGGTCACACTACCTATAGACGTCTTAAGCCGAATCGCACCAGCGCCGGCTCCAGGTA a R I * I G T L ? M H A R A R R Q C D G Y L Q N S A * R G R G R G P S - b G F E L G P S ? C M L E R A A S V M D I C R I R L S V V A A E V H - c D L N W D P L ? A C S S A P P V * W I S A E F G L A W S R P R S I - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d S K F Q S G R ? A H E L A G G T H H I D A S N P K A H D R G L D M - e P N S N P G E ? H M S S R A A L T I S I Q L I R S L T T A A S T W - f I Q I P V R * ? C A R A R R W H S P Y R C F E A * R P R P R P G - XcmI BseRI| BcgI EcoRI BcgI AceIII BsrDI || | | | | | || CACCAAAGGCAGGAGAGCGAATTCTTTGCCGCCACCCGTTCCTTGAATCCAAGAGGGCTTATGTTGTCCCCCAGCATGTTGAGGAGCTGCCACATTGCTA 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 GTGGTTTCCGTCCTCTCGCTTAAGAAACGGCGGTGGGCAAGGAACTTAGGTTCTCCCGAATACAACAGGGGGTCGTACAACTCCTCGACGGTGTAACGAT a P K A G E R I L C R H P F L E S K R A Y V V P Q H V E E L P H C Y - b H Q R Q E S E F F A A T R S L N P R G L M L S P S M L R S C H I A T - c T K G R R A N S L P P P V P * I Q E G L C C P P A C * G A A T L L - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d V L P L L A F E K G G G T G Q I W S P K H Q G G A H Q P A A V N S - e * W L C S L S N K A A V R E K F G L P S I N D G L M N L L Q W M A - f D G F A P S R I R Q R W G N R S D L L A * T T G W C T S S S G C Q * - EagI AceIII BplI AceIII | | | | | CTGGCCTGGGAGTTCAGATAAACCTAGAGCAGAGCTGCCCTCGCTGGTGAAGATCAGAAGCCGCTGTATGCAACAGCTCGAAAAACTGCGGCCGGATCAC 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 GACCGGACCCTCAAGTCTATTTGGATCTCGTCTCGACGGGAGCGACCACTTCTAGTCTTCGGCGACATACGTTGTCGAGCTTTTTGACGCCGGCCTAGTG a W P G S S D K P R A E L P S L V K I R S R C M Q Q L E K L R P D H - b G L G V Q I N L E Q S C P R W * R S E A A V C N S S K N C G R I T - c L A W E F R * T * S R A A L A G E D Q K P L Y A T A R K T A A G S H - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d S A Q S N L Y V * L L A A R A P S S * F G S Y A V A R F V A A P D - e V P R P T * I F R S C L Q G R Q H L D S A A T H L L E F F Q P R I V - f Q G P L E S L G L A S S G E S T F I L L R Q I C C S S F S R G S * - EagI NotI BglI| BspMI|| SmaI ||| BsbI HgiEII SrfI |||BsrBI EcoRI BstXI EagI | | | ||| | | | | ATCAGGCGGCTAAATCCAACACCTTACAAGGTGAGTGTGAGTGCAAAGCTGTACCTGCCCGGGCGGCCGCTCGAAAGCCGAATTCCAGCACACTGGCGGC 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 TAGTCCGCCGATTTAGGTTGTGGAATGTTCCACTCACACTCACGTTTCGACATGGACGGGCCCGCCGGCGAGCTTTCGGCTTAAGGTCGTGTGACCGCCG a I R R L N P T P Y K V S V S A K L Y L P G R P L E S R I P A H W R P - b S G G * I Q H L T R * V * V Q S C T C P G G R S K A E F Q H T G G - c Q A A K S N T L Q G E C E C K A V P A R A A A R K P N S S T L A A - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d C * A A L D L V K C P S H S H L A T G A R A A A R F G F E L V S A A - e D P P * I W C R V L H T H T C L Q V Q G P P R E F A S N W C V P P - f M L R S F G V G * L T L T L A F S Y R G P R G S S L R I G A C Q R - HindIII SpeI BamHI SacI KpnI | BsrBI | | | | | | CGTTACTAGTGGATCCGAGCTCGGTACCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACAT 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 GCAATGATCACCTAGGCTCGAGCCATGGTTCGAACCGCATTAGTACCAGTATCGACAAAGGACACACTTTAACAATAGGCGAGTGTTAAGGTGTGTTGTA a L L V D P S S V P S L A * S W S * L F P V * N C Y P L T I P H N I - b R Y * W I R A R Y Q A W R N H G H S C F L C E I V I R S Q F H T T Y - c V T S G S E L G T K L G V I M V I A V S C V K L L S A H N S T Q H - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d T V L P D S S P V L S P T I M T M A T E Q T F N N D A * L E V C C - e R * * H I R A R Y W A Q R L * P * L Q K R H S I T I R E C N W V V - f G N S T S G L E T G L K A Y D H D Y S N G T H F Q * G S V I G C L M - VspI BspGI | | ACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTGTCCAGTCGGGAAAC 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 TGCTCGGCCTTCGTATTTCACATTTCGGACCCCACGGATTACTCACTCGATTGAGTGTAATTAACGCAACGCGAGTGACGGGCGACAGGTCAGCCCTTTG a R A G S I K C K A W G A * * V S * L T L I A L R S L P A V Q S G N - b E P E A * S V K P G V P N E * A N S H * L R C A H C P L S S R E T - c T S R K H K V * S L G C L M S E L T H I N C V A L T A R C P V G K P - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d V L R F C L T Y L R P H R I L S S V * M L Q T A S V A R Q G T P F - e Y S G S A Y L T F G P T G L S H A L E C * N R Q A * Q G S D L R S V - f R A P L M F H L A Q P A * H T L * S V N I A N R E S G A T W D P F - EarI PvuII VspI SapI | | | CTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGAGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCT 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 GACAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCTCTCTCCGCCAAACGCATAACCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGA a L S C Q L H * * I G Q R A E R G G L R I G R S S A S S L T D S L R S - b C R A S C I N E S A N A R R E A V C V L G A L P L P R S L T R C A - c V V P A A L M N R P T R G E R R F A Y W A L F R F L A H * L A A L - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d G T T G A A N I F R G V R P S L R N A Y Q A S K R K R A * Q S A A S - e Q R A L Q M L S D A L A R L S A T Q T N P A R G S G R E S V R Q A - f R D H W S C * H I P W R A S L P P K R I P R E E A E E S V S E S R - Bst1107I BsrBI AceIII | BspLU11I | | | | CGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGCCGGTATACGGTTATCCACAGGATCNGGGGATAACGCAGGAAAGAACATGTGAGCAAAA 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 GCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCGGCCATATGCCAATAGGTGTCCTAGNCCCCTATTGCGTCCTTTCTTGTACACTCGTTTT a V V R L R R A V S A H S K P V Y G Y P Q D ? G I T Q E R T C E Q K - b R S F G C G E R Y Q L T Q S R Y T V I H R I ? G * R R K E H V S K K - c G R S A A A S G I S S L K A G I R L S T G S G D N A G K N M * A K - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d P R E A A A L P I L E S L A P I R N D V P D P S L A P F F M H A F - e R D N P Q P S R Y * S V * L R Y V T I W L I ? P Y R L F S C T L L - f E T T R S R R A T D A * E F G T Y P * G C S ? P I V C S L V H S C F - AGCCAGCAAAAGGCAGGAACCGTNAAAAGGCCGGGTGNTTGGCGGTTTTACCATAGGCTCGGCCCCCTGACGAGCATACCAAAAATGACGCTCAGTTA 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+-------- 898 TCGGTCGTTTTCCGTCCTTGGCANTTTTCCGGCCCACNAACCGCCAAAATGGTATCCGAGCCGGGGGACTGCTCGTATGGTTTTTACTGCGAGTCAAT a A S K R Q E P ? K G R V ? G G F T I G S A P * R A Y Q K * R S V - b P A K G R N R ? K A G ? L A V L P * A R P P D E H T K N D A Q L - c S Q Q K A G T V K R P G ? W R F Y H R L G P L T S I P K M T L S - 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+-------- 898 d L W C F A P V T ? L G P ? Q R N * W L S P G R V L M G F I V S L * - e F G A F P L F R ? F A P H ? A T K G Y A R G G S S C V L F S A * N - f A L L L C S G ? F P R T ? P P K V M P E A G Q R A Y W F H R E T - Enzymes that do cut: AceIII BamHI BcgI BglI BplI Bpu1102I BsaBI BsbI BseRI BspGI BspLU11I BspMI BsrBI BsrDI BssHII Bst1107I BstXI EagI EarI EcoRI EcoRV HgiEII HindIII KpnI NotI NsiI PstI PvuII SacI SanDI SapI SmaI SpeI SphI SrfI VspI XcmI XhoI Enzymes that do not cut: AatII AflII AhdI AlwNI ApaI ApaBI ApaLI AscI AvrII BbsI Bce83I BclI BfiI BglII BpmI Bpu10I BsaI BsaXI BsgI BsiWI BsmI BsmBI BspEI BsrGI BssSI BstEII Bsu36I ClaI DraI DraIII DrdI DrdII EciI Eco47III Eco57I EcoNI FseI FspI HpaI MfeI MluI MscI NarI NcoI NdeI NgoAIV NheI NruI NspV PacI Pfl1108I PflMI PinAI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SexAI SfiI SgfI SgrAI SnaBI Sse8387I Sse8647I SspI StuI SwaI Tth111I XbaI XmnI