(Linear) MAP of: ./Fgr-S_1_F02_T7.seq check: 4371 from: 1 to: 958 Fgr-S_1_F02_T7 958 85 536 ABI Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * July 30, 2001 22:34 .. BglI FseI | EagI | | NgoAIV | | | EagI | | | |BglI EciI PflMI | | | | | | | | GGGCTGCGGCCGGCCGGGCTGGCGAGGGCGGAGCGNGTGCCTTGCCNGNAGCNGATCTGTAGTGACATCTATTTGTGCTCGGACGCCGCCATGTGATGGT 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 CCCGACGCCGGCCGGCCCGACCGCTCCCGCCTCGCNCACGGAACGGNCNTCGNCTAGACATCACTGTAGATAAACACGAGCCTGCGGCGGTACACTACCA a G L R P A G L A R A E R V P C ? ? ? I C S D I Y L C S D A A M * W Y - b G C G R P G W R G R S ? C L A ? S ? S V V T S I C A R T P P C D G - c A A A G R A G E G G A ? A L P ? A D L * * H L F V L G R R H V M V - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d A A A P R A P S P P A ? A K G ? A S R Y H C R N T S P R R W T I T - e P Q P R G P Q R P R L ? H R A ? L ? D T T V D I Q A R V G G H S P - f S R G A P S A L A S R T G Q ? ? ? I Q L S M * K H E S A A M H H - EcoRI PstI|Bpu1102I EagI KpnI || | | | ATCTGCAGAATTCGGCTTAGCGTGGTCGCGGCCGAGGTACCCTTGTTGCCCTTGTCCTGGTTGTTGCACTTGTCCTATTTGTTGGCCTTGTCCTGGTTGT 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 TAGACGTCTTAAGCCGAATCGCACCAGCGCCGGCTCCATGGGAACAACGGGAACAGGACCAACAACGTGAACAGGATAAACAACCGGAACAGGACCAACA a L Q N S A * R G R G R G T L V A L V L V V A L V L F V G L V L V V - b I C R I R L S V V A A E V P L L P L S W L L H L S Y L L A L S W L L - c S A E F G L A W S R P R Y P C C P C P G C C T C P I C W P C P G C - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d D A S N P K A H D R G L Y G Q Q G Q G P Q Q V Q G I Q Q G Q G P Q - e I Q L I R S L T T A A S T G K N G K D Q N N C K D * K N A K D Q N - f Y R C F E A * R P R P R P V R T A R T R T T A S T R N T P R T R T T - TGCGCTTGTCCTGACTGCTGCGGGGACGTTGGGCAGTGCCCTTGTTGCCCTTGTCCTGGTTGTTGCCCTTGTCCTGGCTGCTGCGGAGAAGTTGGGTAGT 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 ACGCGAACAGGACTGACGACGCCCCTGCAACCCGTCACGGGAACAACGGGAACAGGACCAACAACGGGAACAGGACCGACGACGCCTCTTCAACCCATCA a A L V L T A A G T L G S A L V A L V L V V A L V L A A A E K L G S - b R L S * L L R G R W A V P L L P L S W L L P L S W L L R R S W V V - c C A C P D C C G D V G Q C P C C P C P G C C P C P G C C G E V G * Y - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d Q A Q G S Q Q P S T P C H G Q Q G Q G P Q Q G Q G P Q Q P S T P Y - e N R K D Q S S R P R Q A T G K N G K D Q N N G K D Q S S R L L Q T T - f A S T R V A A P V N P L A R T A R T R T T A R T R A A A S F N P L - EagI NotI BglI| BspMI|| SmaI ||| SrfI |||BsrBI EcoRI | ||| | | ACCCTTGTTGCCCTTGTCCTAGTTGTTGCCCTTGTCCTGGCTGCTGTGGAGAAGTTGGATAGTACCTGCCCGGGCGGCCGCTCGAAAGCCGAATTCCAGC 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 TGGGAACAACGGGAACAGGATCAACAACGGGAACAGGACCGACGACACCTCTTCAACCTATCATGGACGGGCCCGCCGGCGAGCTTTCGGCTTAAGGTCG a T L V A L V L V V A L V L A A V E K L D S T C P G G R S K A E F Q H - b P L L P L S * L L P L S W L L W R S W I V P A R A A A R K P N S S - c P C C P C P S C C P C P G C C G E V G * Y L P G R P L E S R I P A - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d Y G Q Q G Q G L Q Q G Q G P Q Q P S T P Y Y R G P R G S S L R I G A - e G K N G K D * N N G K D Q S S H L L Q I T G A R A A A R F G F E L - f V R T A R T R T T A R T R A A T S F N S L V Q G P P R E F A S N W - HindIII BstXI EagI SpeI BamHI SacI KpnI | BsrBI | | | | | | | | ACACTGGCGGCCGTTACTAGTGGATCCGAGCTCGGTACCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATT 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 TGTGACCGCCGGCAATGATCACCTAGGCTCGAGCCATGGTTCGAACCGCATTAGTACCAGTATCGACAAAGGACACACTTTAACAATAGGCGAGTGTTAA a T G G R Y * W I R A R Y Q A W R N H G H S C F L C E I V I R S Q F - b T L A A V T S G S E L G T K L G V I M V I A V S C V K L L S A H N S - c H W R P L L V D P S S V P S L A * S W S * L F P V * N C Y P L T I - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d C Q R G N S T S G L E T G L K A Y D H D Y S N G T H F Q * G S V I - e V S A A T V L P D S S P V L S P T I M T M A T E Q T F N N D A * L - f C V P P R * * H I R A R Y W A Q R L * P * L Q K R H S I T I R E C N - VspI | CCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGNGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCC 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 GGTGTGTTGTATGCTCGGCCTTCGTATTTCACATTTCGGACNCCACGGATTACTCACTCGATTGAGTGTAATTAACGCAACGCGAGTGACGGGCGAAAGG a H T T Y E P E A * S V K P ? V P N E * A N S H * L R C A H C P L S - b T Q H T S R K H K V * S L ? C L M S E L T H I N C V A L T A R F P - c P H N I R A G S I K C K A ? G A * * V S * L T L I A L R S L P A F Q - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d G C L M R A P L M F H L A Q P A * H T L * S V N I A N R E S G A K - e E V C C V L R F C L T Y L R ? H R I L S S V * M L Q T A S V A R K G - f W V V Y S G S A Y L T F G ? T G L S H A L E C * N R Q A * Q G S E - EarI PvuII VspI BsaXI SapI | | | | AGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCCTCGTCACTGA 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 TCAGCCCTTTGGACAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCCCTCTCCGCCAAACGCATAACCCGCGAGAAGGCGAAGGGAGCAGTGACT a S R E T C R A S C I N E S A N A R G E A V C V L G A L P L P S S L T - b V G K P V V P A A L M N R P T R G E R R F A Y W A L F R F P R H * - c S G N L S C Q L H * * I G Q R A G R G G L R I G R S S A S L V T D - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d W D P F R D H W S C * H I P W R A P L P P K R I P R E E A E R T V S - e T P F G T T G A A N I F R G V R P S L R N A Y Q A S K R K G R * Q - f L R S V Q R A L Q M L S D A L A R P S A T Q T N P A R G S G E D S - AceIII | CTCGCTGTGCTCGGTCGTTCGGCTGCGGCAAGCGGGATCAGCTCACTCAAAGGCGTTAATCCGGTTTTCCACAGAATCCGGGGGATTACGCAGGAAAAAC 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 GAGCGACACGAGCCAGCAAGCCGACGCCGTTCGCCCTAGTCGAGTGAGTTTCCGCAATTAGGCCAAAAGGTGTCTTAGGCCCCCTAATGCGTCCTTTTTG a R C A R S F G C G K R D Q L T Q R R * S G F P Q N P G D Y A G K T - b L A V L G R S A A A S G I S S L K G V N P V F H R I R G I T Q E K P - c S L C S V V R L R Q A G S A H S K A L I R F S T E S G G L R R K N - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d E S H E T T R S R C A P D A * E F A N I R N E V S D P P N R L F F - e S A T S P R E A A A L P I L E S L P T L G T K W L I R P I V C S F - f V R Q A R D N P Q P L R S * S V * L R * D P K G C F G P S * A P F V - DrdII | CTGTGAACCAAAGGGCAACAAAAGGCCAGGAACGGGAAAAAGGCCGGTTGGCTGGGTTTTTTACTTAGGTTCCCCCCCCCTTACAAATCTTCCCAAAAAT 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 GACACTTGGTTTCCCGTTGTTTTCCGGTCCTTGCCCTTTTTCCGGCCAACCGACCCAAAAAATGAATCCAAGGGGGGGGGAATGTTTAGAAGGGTTTTTA a C E P K G N K R P G T G K R P V G W V F Y L G S P P L T N L P K N - b V N Q R A T K G Q E R E K G R L A G F F T * V P P P L Q I F P K I - c L * T K G Q Q K A R N G K K A G W L G F L L R F P P P Y K S S Q K S - 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 d R H V L P C C F A L F P F F A P Q S P K K S L N G G G * L D E W F - e G T F W L A V F P W S R S F P R N A P N K V * T G G G K C I K G F I - f Q S G F P L L L G P V P F L G T P Q T K * K P E G G R V F R G L F - AflII | CCCCCCTTAAGTTCAAAAGTGGGCGAAAACCCTCACCGGACTTTTATAGAATCCCCCT 901 ---------+---------+---------+---------+---------+-------- 958 GGGGGGAATTCAAGTTTTCACCCGCTTTTGGGAGTGGCCTGAAAATATCTTAGGGGGA a P P L S S K V G E N P H R T F I E S P - b P P * V Q K W A K T L T G L L * N P P - c P L K F K S G R K P S P D F Y R I P - 901 ---------+---------+---------+---------+---------+-------- 958 d D G R L N L L P R F G E G S K * L I G R - e G G * T * F H A F V R V P S K Y F G G - f G G K L E F T P S F G * R V K I S D G - Enzymes that do cut: AceIII AflII BamHI BglI Bpu1102I BsaXI BspMI BsrBI BstXI DrdII EagI EarI EciI EcoRI FseI HindIII KpnI NgoAIV NotI PflMI PstI PvuII SacI SapI SmaI SpeI SrfI VspI Enzymes that do not cut: AatII AhdI AlwNI ApaI ApaBI ApaLI AscI AvrII BbsI Bce83I BcgI BclI BfiI BglII BplI BpmI Bpu10I BsaI BsaBI BsbI BseRI BsgI BsiWI BsmI BsmBI BspEI BspGI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstEII Bsu36I ClaI DraI DraIII DrdI Eco47III Eco57I EcoNI EcoRV FspI HgiEII HpaI MfeI MluI MscI NarI NcoI NdeI NheI NruI NsiI NspV PacI Pfl1108I PinAI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SanDI ScaI SexAI SfiI SgfI SgrAI SnaBI SphI Sse8387I Sse8647I SspI StuI SwaI Tth111I XbaI XcmI XhoI XmnI