(Linear) MAP of: ./Fgr-S_2_B04_T7.seq check: 5048 from: 1 to: 909 Fgr-S_2_B04_T7 909 42 615 ABI Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * July 30, 2001 22:34 .. XhoI EcoRV BspMI BglI SphI| BsaBI | EcoRI EagI | SmaI NspV SanDI NsiI || EagI BstXI | | PstI|Bpu1102I NotI | SrfI | | | || | | | | || | | | | GGGGATTCGAATTGGGACCCTCTATATGCATGCTCGAGNCGGCCGCCAGTGTGATGGATATCTGCAGAATTCGGCTTAGCGTGGTCGCGGCCGCCCGGGC 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 CCCCTAAGCTTAACCCTGGGAGATATACGTACGAGCTCNGCCGGCGGTCACACTACCTATAGACGTCTTAAGCCGAATCGCACCAGCGCCGGCGGGCCCG a G D S N W D P L Y A C S ? R P P V * W I S A E F G L A W S R P P G Q - b G I R I G T L Y M H A R ? G R Q C D G Y L Q N S A * R G R G R P G - c G F E L G P S I C M L E ? A A S V M D I C R I R L S V V A A A R A - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d P I R I P V R * I C A R ? P R W H S P Y R C F E A * R P R P R G P - e S E F Q S G R Y A H E L R G G T H H I D A S N P K A H D R G G P - f P N S N P G E I H M S S ? A A L T I S I Q L I R S L T T A A A R A - AGGTACTATCCAACTTCTCCACAGCAGCCAGGACAAGGGCAACAACTAGGACAAGGGCAACAAGGGTACTACCCAACTTCTCCGCAGCAGCCAGGACAAG 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 TCCATGATAGGTTGAAGAGGTGTCGTCGGTCCTGTTCCCGTTGTTGATCCTGTTCCCGTTGTTCCCATGATGGGTTGAAGAGGCGTCGTCGGTCCTGTTC a V L S N F S T A A R T R A T T R T R A T R V L P N F S A A A R T R - b R Y Y P T S P Q Q P G Q G Q Q L G Q G Q Q G Y Y P T S P Q Q P G Q G - c G T I Q L L H S S Q D K G N N * D K G N K G T T Q L L R S S Q D K - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d L Y * G V E G C C G P C P C C S P C P C C P Y * G V E G C C G P C - e C T S D L K E V A A L V L A V V L V L A V L T S G L K E A A A L V L - f P V I W S R W L L W S L P L L * S L P L L P V V W S R R L L W S L - GGCAACAACCAGGACAAGGGCAACAAGGGCACTGCCCAACGTCCCCGCAGCAGTCAGGACAAGCGCAACAACCAGGACAAGGCCAACAAATAGGACAAGT 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 CCGTTGTTGGTCCTGTTCCCGTTGTTCCCGTGACGGGTTGCAGGGGCGTCGTCAGTCCTGTTCGCGTTGTTGGTCCTGTTCCGGTTGTTTATCCTGTTCA a A T T R T R A T R A L P N V P A A V R T S A T T R T R P T N R T S - b Q Q P G Q G Q Q G H C P T S P Q Q S G Q A Q Q P G Q G Q Q I G Q V - c G N N Q D K G N K G T A Q R P R S S Q D K R N N Q D K A N K * D K C - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d P C C G P C P C C P C Q G V D G C C D P C A C C G P C P W C I P C T - e A V V L V L A V L A S G L T G A A T L V L A V V L V L G V F L V L - f P L L W S L P L L P V A W R G R L L * S L R L L W S L A L L Y S L - EagI KpnI | Bpu1102I EcoRI BstXI EagI SpeI BamHI SacI | | | | | | | | | GCAACAACCAGGACAAGGGCAACAAGGGTACCTCGGCCGCGACCACGCTAAGCCGAATTCCAGCACACTGGCGGCCGTTACTAGTGGATCCGAGCTCGGT 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 CGTTGTTGGTCCTGTTCCCGTTGTTCCCATGGAGCCGGCGCTGGTGCGATTCGGCTTAAGGTCGTGTGACCGCCGGCAATGATCACCTAGGCTCGAGCCA a A T T R T R A T R V P R P R P R * A E F Q H T G G R Y * W I R A R Y - b Q Q P G Q G Q Q G Y L G R D H A K P N S S T L A A V T S G S E L G - c N N Q D K G N K G T S A A T T L S R I P A H W R P L L V D P S S V - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d C C G P C P C C P Y R P R S W A L G F E L V S A A T V L P D S S P - e A V V L V L A V L T G R G R G R * A S N W C V P P R * * H I R A R - f H L L W S L P L L P V E A A V V S L R I G A C Q R G N S T S G L E T - HindIII KpnI | BsrBI | | | ACCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAA 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 TGGTTCGAACCGCATTAGTACCAGTATCGACAAAGGACACACTTTAACAATAGGCGAGTGTTAAGGTGTGTTGTATGCTCGGCCTTCGTATTTCACATTT a Q A W R N H G H S C F L C E I V I R S Q F H T T Y E P E A * S V K - b T K L G V I M V I A V S C V K L L S A H N S T Q H T S R K H K V * S - c P S L A * S W S * L F P V * N C Y P L T I P H N I R A G S I K C K - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d V L S P T I M T M A T E Q T F N N D A * L E V C C V L R F C L T Y - e Y W A Q R L * P * L Q K R H S I T I R E C N W V V Y S G S A Y L T F - f G L K A Y D H D Y S N G T H F Q * G S V I G C L M R A P L M F H L - VspI PvuII VspI | | | GCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAA 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 CGGACCCCACGGATTACTCACTCGATTGAGTGTAATTAACGCAACGCGAGTGACGGGCGAAAGGTCAGCCCTTTGGACAGCACGGTCGACGTAATTACTT a P G V P N E * A N S H * L R C A H C P L S S R E T C R A S C I N E - b L G C L M S E L T H I N C V A L T A R F P V G K P V V P A A L M N - c A W G A * * V S * L T L I A L R S L P A F Q S G N L S C Q L H * * I - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d L R P H R I L S S V * M L Q T A S V A R K G T P F G T T G A A N I F - e G P T G L S H A L E C * N R Q A * Q G S E L R S V Q R A L Q M L S - f A Q P A * H T L * S V N I A N R E S G A K W D P F R D H W S C * H - EarI BsaXI SapI BsrBI | | | TCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGT 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 AGCCGGTTGCGCGCCCCTCTCCGCCAAACGCATAACCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCA a S A N A R G E A V C V L G A L P L P R S L T R C A R S F G C G E R Y - b R P T R G E R R F A Y W A L F R F L A H * L A A L G R S A A A S G - c G Q R A G R G G L R I G R S S A S S L T D S L R S V V R L R R A V - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d R G V R P S L R N A Y Q A S K R K R A * Q S A A S P R E A A A L P - e D A L A R P S A T Q T N P A R G S G R E S V R Q A R D N P Q P S R - f I P W R A P L P P K R I P R E E A E E S V S E S R E T T R S R R A T - AceIII BspLU11I | | ATCAGCTCACTCAAAGGCGGNAATACCGTTATTCACAGAATCAGGGGGATAACCCAGGGAAGAACATGTTGACCCAAAGGGCCGCCAAAAGGCCAGAACC 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 TAGTCGAGTGAGTTTCCGCCNTTATGGCAATAAGTGTCTTAGTCCCCCTATTGGGTCCCTTCTTGTACAACTGGGTTTCCCGGCGGTTTTCCGGTCTTGG a Q L T Q R R ? Y R Y S Q N Q G D N P G K N M L T Q R A A K R P E P - b I S S L K G G N T V I H R I R G I T Q G R T C * P K G P P K G Q N P - c S A H S K A ? I P L F T E S G G * P R E E H V D P K G R Q K A R T - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d I L E S L P P ? V T I * L I L P I V W P L V H Q G L P G G F P W F - e Y * S V * L R ? Y R * E C F * P S L G P F F M N V W L A A L L G S G - f D A * E F A ? I G N N V S D P P Y G L S S C T S G F P R W F A L V - BglI AvrII | | CGTAAAAGGGCCGGGTTGGCTGGCCGGTTTTCCCCTAGGGCTCCCGCCCCCCTTGGCCGAACCTTCCCAAAAAATCCGAACCCCTCAAATTCAAAAGGGT 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 GCATTTTCCCGGCCCAACCGACCGGCCAAAAGGGGATCCCGAGGGCGGGGGGAACCGGCTTGGAAGGGTTTTTTAGGCTTGGGGAGTTTAAGTTTTCCCA a V K G P G W L A G F P L G L P P P L A E P S Q K I R T P Q I Q K G - b * K G R V G W P V F P * G S R P P W P N L P K K S E P L K F K R V - c R K R A G L A G R F S P R A P A P L G R T F P K N P N P S N S K G W - 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 d G Y F P R T P Q G T K G * P E R G G Q G F R G L F D S G R L N L L T - e T F P G P Q S A P K G R P S G G G K A S G E W F I R V G * I * F P - f R L L A P N A P R N E G L A G A G R P R V K G F F G F G E F E F P - GGCCGAAAA 901 --------- 909 CCGGCTTTT a G R K - b A E - c P K - 901 --------- 909 d A S F - e P R F - f H G F - Enzymes that do cut: AceIII AvrII BamHI BglI Bpu1102I BsaBI BsaXI BspLU11I BspMI BsrBI BstXI EagI EarI EcoRI EcoRV HindIII KpnI NotI NsiI NspV PstI PvuII SacI SanDI SapI SmaI SpeI SphI SrfI VspI XhoI Enzymes that do not cut: AatII AflII AhdI AlwNI ApaI ApaBI ApaLI AscI BbsI Bce83I BcgI BclI BfiI BglII BplI BpmI Bpu10I BsaI BsbI BseRI BsgI BsiWI BsmI BsmBI BspEI BspGI BsrDI BsrGI BssHII BssSI Bst1107I BstEII Bsu36I ClaI DraI DraIII DrdI DrdII EciI Eco47III Eco57I EcoNI FseI FspI HgiEII HpaI MfeI MluI MscI NarI NcoI NdeI NgoAIV NheI NruI PacI Pfl1108I PflMI PinAI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SexAI SfiI SgfI SgrAI SnaBI Sse8387I Sse8647I SspI StuI SwaI Tth111I XbaI XcmI XmnI