(Linear) MAP of: ./Fgr-S_3_A11_T7.seq check: 457 from: 1 to: 887 Fgr-S_3_A11_T7 887 25 732 ABI Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * July 30, 2001 22:34 .. EagI NotI EcoRV BsrBI| BsaBI | EcoRI XbaI XhoI || BstXI | | PstI|Bpu1102I EagI | | || | | | || | | GGGGGGCTTGGGGCCTCTAGATGCTGCTCGAGCGGCCGCCAGTGTGATGGATATCTGCAGAATTCGGCTTAGCGTGGTCGCGGCCGAGGTGGGATATAAT 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 CCCCCCGAACCCCGGAGATCTACGACGAGCTCGCCGGCGGTCACACTACCTATAGACGTCTTAAGCCGAATCGCACCAGCGCCGGCTCCACCCTATATTA a G G L G A S R C C S S G R Q C D G Y L Q N S A * R G R G R G G I * W - b G G L G P L D A A R A A A S V M D I C R I R L S V V A A E V G Y N - c G A W G L * M L L E R P P V * W I S A E F G L A W S R P R W D I M - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d P S P A E L H Q E L P R W H S P Y R C F E A * R P R P R P P I Y - e P A Q P R * I S S S R G G T H H I D A S N P K A H D R G L H S I I - f P P K P G R S A A R A A A L T I S I Q L I R S L T T A A S T P Y L - BsaXI | GGAGGTAATGTGGATTAGCAGTAGTAATTCCATGTGCTCCATATATAATTGTTTGACCTTGTTTTGTGGATTGACAAGCTGAGATATAATACTTCTCTTT 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 CCTCCATTACACCTAATCGTCATCATTAAGGTACACGAGGTATATATTAACAAACTGGAACAAAACACCTAACTGTTCGACTCTATATTATGAAGAGAAA a R * C G L A V V I P C A P Y I I V * P C F V D * Q A E I * Y F S L - b G G N V D * Q * * F H V L H I * L F D L V L W I D K L R Y N T S L Y - c E V M W I S S S N S M C S I Y N C L T L F C G L T S * D I I L L F - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d H L Y H P N A T T I G H A G Y I I T Q G Q K T S Q C A S I Y Y K E K - e S T I H I L L L L E M H E M Y L Q K V K N Q P N V L Q S I I S R K - f P P L T S * C Y Y N W T S W I Y N N S R T K H I S L S L Y L V E R - SphI NsiI | BsrDI Eco47III | | | | ATTCCATCCCGATGCATGCAGATTTTGAGCAATGGGTTCTTCAAGAGCCTGGTGCATAGGGTTGTGACCAACGCGGAGAAAGAGCGCTTGTCGTTGGTGC 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 TAAGGTAGGGCTACGTACGTCTAAAACTCGTTACCCAAGAAGTTCTCGGACCACGTATCCCAACACTGGTTGCGCCTCTTTCTCGCGAACAGCAACCACG a F H P D A C R F * A M G S S R A W C I G L * P T R R K S A C R W C - b S I P M H A D F E Q W V L Q E P G A * G C D Q R G E R A L V V G A - c I P S R C M Q I L S N G F F K S L V H R V V T N A E K E R L S L V L - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d N W G S A H L N Q A I P E E L A Q H M P N H G V R L F L A Q R Q H - e I G D R H M C I K L L P N K L L R T C L T T V L A S F S R K D N T - f * E M G I C A S K S C H T R * S G P A Y P Q S W R P S L A S T T P A - BsgI Bce83I| SexAI ||BsaI BspMI | || | | TGGTCTATACATTGGAGCTAGAGACAGAGCTTGAGCCGGTGTCAGACCTGGTGGATGACAAGAGACCTGCACGATACATGAAGATCAAGCTCCACGACTA 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 ACCAGATATGTAACCTCGATCTCTGTCTCGAACTCGGCCACAGTCTGGACCACCTACTGTTCTCTGGACGTGCTATGTACTTCTAGTTCGAGGTGCTGAT a W S I H W S * R Q S L S R C Q T W W M T R D L H D T * R S S S T T T - b G L Y I G A R D R A * A G V R P G G * Q E T C T I H E D Q A P R L - c V Y T L E L E T E L E P V S D L V D D K R P A R Y M K I K L H D Y - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d Q D I C Q L * L C L K L R H * V Q H I V L S R C S V H L D L E V V - e S T * V N S S S V S S S G T D S R T S S L L G A R Y M F I L S W S * - f P R Y M P A L S L A Q A P T L G P P H C S V Q V I C S S * A G R S - EagI NotI BglI| BspMI|| SmaI ||| RcaI BglII SrfI |||BsrBI | | | ||| | CATGGAAAAATATCATGACACTTATGCCACAGGGACACTAGCCATCGACGGCGTGAAGATCTGAATATGATTCTGATGTGTACCTGCCCGGGCGGCCGCT 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 GTACCTTTTTATAGTACTGTGAATACGGTGTCCCTGTGATCGGTAGCTGCCGCACTTCTAGACTTATACTAAGACTACACATGGACGGGCCCGCCGGCGA a W K N I M T L M P Q G H * P S T A * R S E Y D S D V Y L P G R P L - b H G K I S * H L C H R D T S H R R R E D L N M I L M C T C P G G R S - c M E K Y H D T Y A T G T L A I D G V K I * I * F * C V P A R A A A - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d V H F F I M V S I G C P C * G D V A H L D S Y S E S T Y R G P R G S - e M S F Y * S V * A V P V S A M S P T F I Q I H N Q H T G A R A A A - f C P F I D H C K H W L S V L W R R R S S R F I I R I H V Q G P P R - HindIII EcoRI BstXI EagI SpeI BamHI SacI KpnI | | | | | | | | | CGAAAGCCGAATTCCAGCACACTGGCGGCCGTTACTAGTGGATCCGAGCTCGGTACCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAAT 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 GCTTTCGGCTTAAGGTCGTGTGACCGCCGGCAATGATCACCTAGGCTCGAGCCATGGTTCGAACCGCATTAGTACCAGTATCGACAAAGGACACACTTTA a E S R I P A H W R P L L V D P S S V P S L A * S W S * L F P V * N - b K A E F Q H T G G R Y * W I R A R Y Q A W R N H G H S C F L C E I - c R K P N S S T L A A V T S G S E L G T K L G V I M V I A V S C V K L - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d S L R I G A C Q R G N S T S G L E T G L K A Y D H D Y S N G T H F - e R F G F E L V S A A T V L P D S S P V L S P T I M T M A T E Q T F - f E F A S N W C V P P R * * H I R A R Y W A Q R L * P * L Q K R H S I - BsrBI VspI | | TGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGC 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 ACAATAGGCGAGTGTTAAGGTGTGTTGTATGCTCGGCCTTCGTATTTCACATTTCGGACCCCACGGATTACTCACTCGATTGAGTGTAATTAACGCAACG a C Y P L T I P H N I R A G S I K C K A W G A * * V S * L T L I A L R - b V I R S Q F H T T Y E P E A * S V K P G V P N E * A N S H * L R C - c L S A H N S T Q H T S R K H K V * S L G C L M S E L T H I N C V A - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d Q * G S V I G C L M R A P L M F H L A Q P A * H T L * S V N I A N - e N N D A * L E V C C V L R F C L T Y L R P H R I L S S V * M L Q T A - f T I R E C N W V V Y S G S A Y L T F G P T G L S H A L E C * N R Q - PvuII VspI BsaXI | | | GCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCNGTTTGCGTATTGGGCGCTCTTC 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 CGAGTGACGGGCGAAAGGTCAGCCCTTTGGACAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCCCTCTCCGNCAAACGCATAACCCGCGAGAAG a S L P A F Q S G N L S C Q L H * * I G Q R A G R G ? L R I G R S S - b A H C P L S S R E T C R A S C I N E S A N A R G E A V C V L G A L P - c L T A R F P V G K P V V P A A L M N R P T R G E R ? F A Y W A L F - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d R E S G A K W D P F R D H W S C * H I P W R A P L P ? K R I P R E E - e S V A R K G T P F G T T G A A N I F R G V R P S L ? N A Y Q A S K - f A * Q G S E L R S V Q R A L Q M L S D A L A R P S A T Q T N P A R - EarI SapI AceIII | | CGCTTNCTCGCTCACTGACTCGCTGCGCTCGGGTCGTTCGCTGCCGCGAGCCGTATCAGCTCACTCAAAGGCGTAATACGGTTATCC 801 ---------+---------+---------+---------+---------+---------+---------+---------+------- 887 GCGAANGAGCGAGTGACTGAGCGACGCGAGCCCAGCAAGCGACGGCGCTCGGCATAGTCGAGTGAGTTTCCGCATTATGCCAATAGG a A ? S L T D S L R S G R S L P R A V S A H S K A * Y G Y - b L ? R S L T R C A R V V R C R E P Y Q L T Q R R N T V I - c R ? L A H * L A A L G S F A A A S R I S S L K G V I R L S - 801 ---------+---------+---------+---------+---------+---------+---------+---------+------- 887 d A ? E S V S E S R E P R E S G R A T D A * E F A Y Y P * G - e R K ? A * Q S A A S P D N A A A L R I L E S L P T I R N D - f G S ? R E S V R Q A R T T R Q R S G Y * S V * L R L V T I - Enzymes that do cut: AceIII BamHI Bce83I BglI BglII Bpu1102I BsaI BsaBI BsaXI BsgI BspMI BsrBI BsrDI BstXI EagI EarI Eco47III EcoRI EcoRV HindIII KpnI NotI NsiI PstI PvuII RcaI SacI SapI SexAI SmaI SpeI SphI SrfI VspI XbaI XhoI Enzymes that do not cut: AatII AflII AhdI AlwNI ApaI ApaBI ApaLI AscI AvrII BbsI BcgI BclI BfiI BplI BpmI Bpu10I BsbI BseRI BsiWI BsmI BsmBI BspEI BspGI BspLU11I BsrGI BssHII BssSI Bst1107I BstEII Bsu36I ClaI DraI DraIII DrdI DrdII EciI Eco57I EcoNI FseI FspI HgiEII HpaI MfeI MluI MscI NarI NcoI NdeI NgoAIV NheI NruI NspV PacI Pfl1108I PflMI PinAI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SanDI ScaI SfiI SgfI SgrAI SnaBI Sse8387I Sse8647I SspI StuI SwaI Tth111I XcmI XmnI