(Linear) MAP of: ./Fgr-S_3_D09_T7.seq check: 4654 from: 1 to: 884 Fgr-S_3_D09_T7 884 25 722 ABI Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * July 30, 2001 22:34 .. EagI NotI EcoRV BsrBI| BsaBI | EcoRI ClaI ApaI XhoI || BstXI | | PstI|Bpu1102I EagI | | | || | | | || | | GGGATCGATTGGGCCCTCTANATGCTGCTCGAGCGGCCGCCAGTGTGATGGATATCTGCAGAATTCGGCTTAGCGTGGTCGCGGCCGAGGTGAGGAAGAC 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 CCCTAGCTAACCCGGGAGATNTACGACGAGCTCGCCGGCGGTCACACTACCTATAGACGTCTTAAGCCGAATCGCACCAGCGCCGGCTCCACTCCTTCTG a G I D W A L ? M L L E R P P V * W I S A E F G L A W S R P R * G R Q - b G S I G P S ? C C S S G R Q C D G Y L Q N S A * R G R G R G E E D - c D R L G P L ? A A R A A A S V M D I C R I R L S V V A A E V R K T - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d I S Q A R * ? S S S R G G T H H I D A S N P K A H D R G L H P L - e S R N P G R ? A A R A A A L T I S I Q L I R S L T T A A S T L F V - f P D I P G E ? H Q E L P R W H S P Y R C F E A * R P R P R P S S S - BbsI SacI BsgI | | | AGGTATGGGAACTCAACCGTGGTGGAGAACACCGAGAAAGGCTGGAAAGAGCTCATCTTCCAGAGGATACGTGCAGTGTTCGGTTTTGGGGTCTGAAAAA 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 TCCATACCCTTGAGTTGGCACCACCTCTTGTGGCTCTTTCCGACCTTTCTCGAGTAGAAGGTCTCCTATGCACGTCACAAGCCAAAACCCCAGACTTTTT a V W E L N R G G E H R E R L E R A H L P E D T C S V R F W G L K K - b R Y G N S T V V E N T E K G W K E L I F Q R I R A V F G F G V * K S - c G M G T Q P W W R T P R K A G K S S S S R G Y V Q C S V L G S E K - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d C T H S S L R P P S C R S L S S L A * R G S S V H L T R N Q P R F F - e P I P V * G H H L V G L F A P F L E D E L P Y T C H E T K P D S F - f L Y P F E V T T S F V S F P Q F S S M K W L I R A T N P K P T Q F - GCATCTTTGAGATCCATCTTCAATGAAGTCGATCCTGCCTTTTCATGGCTATCCATGTTTTGCTGTCTTAGTTTATTCGATGCTGATAGACACACAAAAC 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 CGTAGAAACTCTAGGTAGAAGTTACTTCAGCTAGGACGGAAAAGTACCGATAGGTACAAAACGACAGAATCAAATAAGCTACGACTATCTGTGTGTTTTG a H L * D P S S M K S I L P F H G Y P C F A V L V Y S M L I D T Q N - b I F E I H L Q * S R S C L F M A I H V L L S * F I R C * * T H K T - c A S L R S I F N E V D P A F S W L S M F C C L S L F D A D R H T K L - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d C R Q S G D E I F D I R G K * P * G H K A T K T * E I S I S V C F - e A D K L D M K L S T S G A K E H S D M N Q Q R L K N S A S L C V F - f L M K S I W R * H L R D Q R K M A I W T K S D * N I R H Q Y V C L V - RcaI | TTAGTGAACTTCCGGGAATTGAAAAATACGTGTTACCTTGTATTCATCACGCTGGGTTGTGCTTTGAAGTCGTCACTCATGAAAGTTTGAGCTTGTGACT 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 AATCACTTGAAGGCCCTTAACTTTTTATGCACAATGGAACATAAGTAGTGCGACCCAACACGAAACTTCAGCAGTGAGTACTTTCAAACTCGAACACTGA a L V N F R E L K N T C Y L V F I T L G C A L K S S L M K V * A C D S - b * * T S G N * K I R V T L Y S S R W V V L * S R H S * K F E L V T - c S E L P G I E K Y V L P C I H H A G L C F E V V T H E S L S L * L - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d K T F K R S N F F V H * R T N M V S P Q A K F D D S M F T Q A Q S - e S L S S G P I S F Y T N G Q I * * A P N H K S T T V * S L K L K H S - f * H V E P F Q F I R T V K Y E D R Q T T S Q L R * E H F N S S T V - EagI NotI BglI| BspMI|| SmaI ||| NgoAIV KpnI SrfI |||BsrBI EcoRI | | | ||| | | CATGTAGAGGCCGGCGAAAGATGGTCGCTGAGTGACTATTATTTTTGTTCCTTGCGTCTGTATGACATGGTACCTGCCCGGGCGGCCGCTCGAAAGCCGA 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 GTACATCTCCGGCCGCTTTCTACCAGCGACTCACTGATAATAAAAACAAGGAACGCAGACATACTGTACCATGGACGGGCCCGCCGGCGAGCTTTCGGCT a C R G R R K M V A E * L L F L F L A S V * H G T C P G G R S K A E - b H V E A G E R W S L S D Y Y F C S L R L Y D M V P A R A A A R K P N - c M * R P A K D G R * V T I I F V P C V C M T W Y L P G R P L E S R - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d E H L P R R F I T A S H S N N K N R A D T H C P V Q G P P R E F A S - e M Y L G A F S P R Q T V I I K T G Q T Q I V H Y R G P R G S S L R - f * T S A P S L H D S L S * * K Q E K R R Y S M T G A R A A A R F G - HindIII BstXI EagI SpeI BamHI SacI KpnI | BsrBI | | | | | | | | ATTCCAGCACACTGGCGGCCGTTACTAGTGGATCCGAGCTCGGTACCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGC 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 TAAGGTCGTGTGACCGCCGGCAATGATCACCTAGGCTCGAGCCATGGTTCGAACCGCATTAGTACCAGTATCGACAAAGGACACACTTTAACAATAGGCG a F Q H T G G R Y * W I R A R Y Q A W R N H G H S C F L C E I V I R - b S S T L A A V T S G S E L G T K L G V I M V I A V S C V K L L S A - c I P A H W R P L L V D P S S V P S L A * S W S * L F P V * N C Y P L - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d N W C V P P R * * H I R A R Y W A Q R L * P * L Q K R H S I T I R - e I G A C Q R G N S T S G L E T G L K A Y D H D Y S N G T H F Q * G - f F E L V S A A T V L P D S S P V L S P T I M T M A T E Q T F N N D A - VspI | TCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGNGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCC 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 AGTGTTAAGGTGTGTTGTATGCTCGGCCTTCGTATTTCACATTTCGGACNCCACGGATTACTCACTCGATTGAGTGTAATTAACGCAACGCGAGTGACGG a S Q F H T T Y E P E A * S V K P ? V P N E * A N S H * L R C A H C P - b H N S T Q H T S R K H K V * S L ? C L M S E L T H I N C V A L T A - c T I P H N I R A G S I K C K A ? G A * * V S * L T L I A L R S L P - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d E C N W V V Y S G S A Y L T F G ? T G L S H A L E C * N R Q A * Q - e S V I G C L M R A P L M F H L A Q P A * H T L * S V N I A N R E S G - f * L E V C C V L R F C L T Y L R ? H R I L S S V * M L Q T A S V A - EarI PvuII VspI BsaXI SapI | | | | CGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTNCTCG 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 GCGAAAGGTCAGCCCTTTGGACAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCCCTCTCCGCCAAACGCATAACCCGCGAGAAGGCGAANGAGC a L S S R E T C R A S C I N E S A N A R G E A V C V L G A L P L ? R - b R F P V G K P V V P A A L M N R P T R G E R R F A Y W A L F R ? L A - c A F Q S G N L S C Q L H * * I G Q R A G R G G L R I G R S S A ? S - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d G S E L R S V Q R A L Q M L S D A L A R P S A T Q T N P A R G S ? R - e A K W D P F R D H W S C * H I P W R A P L P P K R I P R E E A ? E - f R K G T P F G T T G A A N I F R G V R P S L R N A Y Q A S K R K ? - BsrBI Bce83I| AceIII || | CTCACTGACTCGCTGCGCTCCGTCGNTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAGGCCGTAATACGGNTATCCACAGATC 801 ---------+---------+---------+---------+---------+---------+---------+---------+---- 884 GAGTGACTGAGCGACGCGAGGCAGCNAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTCCGGCATTATGCCNATAGGTGTCTAG a S L T R C A P S ? G C G E R Y Q L T Q G R N T ? I H R - b H * L A A L R R S A A A S G I S S L K A V I R ? S T D - c L T D S L R S V ? R L R R A V S A H S R P * Y G Y P Q I - 801 ---------+---------+---------+---------+---------+---------+---------+---------+---- 884 d E S V R Q A G D ? P Q P S R Y * S V * P R L V ? I W L D - e S V S E S R E T ? R S R R A T D A * E L G Y Y P ? G C I - f A * Q S A A S R R ? A A A L P I L E S L A T I R ? D V S - Enzymes that do cut: AceIII ApaI BamHI BbsI Bce83I BglI Bpu1102I BsaBI BsaXI BsgI BspMI BsrBI BstXI ClaI EagI EarI EcoRI EcoRV HindIII KpnI NgoAIV NotI PstI PvuII RcaI SacI SapI SmaI SpeI SrfI VspI XhoI Enzymes that do not cut: AatII AflII AhdI AlwNI ApaBI ApaLI AscI AvrII BcgI BclI BfiI BglII BplI BpmI Bpu10I BsaI BsbI BseRI BsiWI BsmI BsmBI BspEI BspGI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstEII Bsu36I DraI DraIII DrdI DrdII EciI Eco47III Eco57I EcoNI FseI FspI HgiEII HpaI MfeI MluI MscI NarI NcoI NdeI NheI NruI NsiI NspV PacI Pfl1108I PflMI PinAI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SanDI ScaI SexAI SfiI SgfI SgrAI SnaBI SphI Sse8387I Sse8647I SspI StuI SwaI Tth111I XbaI XcmI XmnI