(Linear) MAP of: ./Fgr-S_4_B12_T7.seq check: 5169 from: 1 to: 876 Fgr-S_4_B12_T7 876 25 680 ABI Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * July 30, 2001 22:35 .. EagI NotI EcoRV BsrBI| BsaBI | EcoRI ApaI XhoI || BstXI | | PstI|Bpu1102I EagI | | || | | | || | | GGATTCGATTGGGCCCTCTANATGCTGCTCGAGCGGCCGCCAGTGTGATGGATATCTGCAGAATTCGGCTTAGCGTGGTCGCGGCCGAGGTCGCCGCTTG 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 CCTAAGCTAACCCGGGAGATNTACGACGAGCTCGCCGGCGGTCACACTACCTATAGACGTCTTAAGCCGAATCGCACCAGCGCCGGCTCCAGCGGCGAAC a G F D W A L ? M L L E R P P V * W I S A E F G L A W S R P R S P L E - b D S I G P S ? C C S S G R Q C D G Y L Q N S A * R G R G R G R R L - c I R L G P L ? A A R A A A S V M D I C R I R L S V V A A E V A A * - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d S E I P G E ? H Q E L P R W H S P Y R C F E A * R P R P R P R R K - e N S Q A R * ? S S S R G G T H H I D A S N P K A H D R G L D G S - f I R N P G R ? A A R A A A L T I S I Q L I R S L T T A A S T A A Q - HindIII NheI PinAI | | | AACAATAGCATCCTCACGGGCACGGACTTGTGAATCAGCATGAATAAGAAAGAAGCTTCAAAGGCTAGCAGGAAATTTCTATCTTTTACAGACCGGTAGA 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 TTGTTATCGTAGGAGTGCCCGTGCCTGAACACTTAGTCGTACTTATTCTTTCTTCGAAGTTTCCGATCGTCCTTTAAAGATAGAAAATGTCTGGCCATCT a Q * H P H G H G L V N Q H E * E R S F K G * Q E I S I F Y R P V D - b N N S I L T G T D L * I S M N K K E A S K A S R K F L S F T D R * I - c T I A S S R A R T C E S A * I R K K L Q R L A G N F Y L L Q T G R - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d F L L M R V P V S K H I L M F L F S A E F A L L F N R D K V S R Y - e S C Y C G * P C P S T F * C S Y S L L K L P * C S I E I K * L G T S - f V I A D E R A R V Q S D A H I L F F S * L S A P F K * R K C V P L - BglII SpeI BsgI | | | | TTCTCCATTTTCTTCCTATATATATGTCTCTCGTAAGGATTAGTATGTAGACTTGTGTGCTTGTACTAGTTGCTAGTGATGCTTTAGATCTTAGCAACTG 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 AAGAGGTAAAAGAAGGATATATATACAGAGAGCATTCCTAATCATACATCTGAACACACGAACATGATCAACGATCACTACGAAATCTAGAATCGTTGAC a S P F S S Y I Y V S R K D * Y V D L C A C T S C * * C F R S * Q L - b L H F L P I Y M S L V R I S M * T C V L V L V A S D A L D L S N C - c F S I F F L Y I C L S * G L V C R L V C L Y * L L V M L * I L A T A - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d I R W K R G I Y I D R T L I L I Y V Q T S T S T A L S A K S R L L Q - e E G N E E * I Y T E R L S * Y T S K H A Q V L Q * H H K L D * C S - f N E M K K R Y I H R E Y P N T H L S T H K Y * N S T I S * I K A V - NsiI NdeI | | CACCTAGCTACCTCATGGTTATGTATGCATACCTAGTATGTGTATGTAACAGGGAAGCATATGGAGTGGTTTTGCCTCTTTGGTGCATTTGAGACAACTA 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 GTGGATCGATGGAGTACCAATACATACGTATGGATCATACACATACATTGTCCCTTCGTATACCTCACCAAAACGGAGAAACCACGTAAACTCTGTTGAT a H L A T S W L C M H T * Y V Y V T G K H M E W F C L F G A F E T T R - b T * L P H G Y V C I P S M C M * Q G S I W S G F A S L V H L R Q L - c P S Y L M V M Y A Y L V C V C N R E A Y G V V L P L W C I * D N * - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d V * S G * P * T H M G L I H I Y C P L M H L P K A E K T C K L C S - e C R A V E H N H I C V * Y T Y T V P F C I S H N Q R K P A N S V V - f A G L * R M T I Y A Y R T H T H L L S A Y P T T K G R Q H M Q S L * - EagI NotI BglI| BspMI|| SmaI ||| HindIII SrfI |||BsrBI EcoRI BstXI EagI SpeI BamHI SacI KpnI | | ||| | | | | | | | | | GATGCCTACTGTACCTGCCCGGGCGGCCGCTCGAAAGCCGAATTCCAGCACACTGGCGGCCGTTACTAGTGGATCCGAGCTCGGTACCAAGCTTGGCGTA 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 CTACGGATGACATGGACGGGCCCGCCGGCGAGCTTTCGGCTTAAGGTCGTGTGACCGCCGGCAATGATCACCTAGGCTCGAGCCATGGTTCGAACCGCAT a C L L Y L P G R P L E S R I P A H W R P L L V D P S S V P S L A * - b D A Y C T C P G G R S K A E F Q H T G G R Y * W I R A R Y Q A W R N - c M P T V P A R A A A R K P N S S T L A A V T S G S E L G T K L G V - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d S A * Q V Q G P P R E F A S N W C V P P R * * H I R A R Y W A Q R - e L H R S Y R G P R G S S L R I G A C Q R G N S T S G L E T G L K A Y - f I G V T G A R A A A R F G F E L V S A A T V L P D S S P V L S P T - BsrBI | ATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAA 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 TAGTACCAGTATCGACAAAGGACACACTTTAACAATAGGCGAGTGTTAAGGTGTGTTGTATGCTCGGCCTTCGTATTTCACATTTCGGACCCCACGGATT a S W S * L F P V * N C Y P L T I P H N I R A G S I K C K A W G A * - b H G H S C F L C E I V I R S Q F H T T Y E P E A * S V K P G V P N - c I M V I A V S C V K L L S A H N S T Q H T S R K H K V * S L G C L M - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d L * P * L Q K R H S I T I R E C N W V V Y S G S A Y L T F G P T G L - e D H D Y S N G T H F Q * G S V I G C L M R A P L M F H L A Q P A * - f I M T M A T E Q T F N N D A * L E V C C V L R F C L T Y L R P H R - VspI PvuII VspI | | | TGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGG 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 ACTCACTCGATTGAGTGTAATTAACGCAACGCGAGTGACGGGCGAAAGGTCAGCCCTTTGGACAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCC a * V S * L T L I A L R S L P A F Q S G N L S C Q L H * * I G Q R A G - b E * A N S H * L R C A H C P L S S R E T C R A S C I N E S A N A R - c S E L T H I N C V A L T A R F P V G K P V V P A A L M N R P T R G - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d S H A L E C * N R Q A * Q G S E L R S V Q R A L Q M L S D A L A R - e H T L * S V N I A N R E S G A K W D P F R D H W S C * H I P W R A - f I L S S V * M L Q T A S V A R K G T P F G T T G A A N I F R G V R P - EarI SapI BsrBI | | GGAGAGGCNNGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGNTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCA 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 CCTCTCCGNNCAAACGCATAACCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCNAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGT a R G ? F A Y W A L F R F L A H * L A A L G S F G C G E R Y Q L T ? - b G E A ? L R I G R S S A S S L T D S L R S ? R S A A A S G I S S L ? - c E R ? V C V L G A L P L P R S L T R C A R ? V R L R R A V S A H S - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d P S A ? K R I P R E E A E E S V S E S R E ? R E A A A L P I L E S - e P L P ? N A Y Q A S K R K R A * Q S A A S P ? N P Q P S R Y * S V * - f S L ? T Q T N P A R G S G R E S V R Q A R ? T R S R R A T D A * E - Bst1107I AceIII | BspLU11I | | | NAGGGCGGTATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAGGC 801 ---------+---------+---------+---------+---------+---------+---------+------ 876 NTCCCGCCATATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTCCG a R A V Y G Y P Q N Q G I T Q E R T C E Q K A S K G - b G R Y T V I H R I R G * R R K E H V S K R P A K - c ? G G I R L S T E S G D N A G K N M * A K G Q Q R - 801 ---------+---------+---------+---------+---------+---------+---------+------ 876 d ? P R Y V T I W L I L P Y R L F S C T L L L G A F A - e L A T Y P * G C F * P I V C S L V H S C F A L L P - f ? P P I R N D V S D P S L A P F F M H A F P W C L - Enzymes that do cut: AceIII ApaI BamHI BglI BglII Bpu1102I BsaBI BsgI BspLU11I BspMI BsrBI Bst1107I BstXI EagI EarI EcoRI EcoRV HindIII KpnI NdeI NheI NotI NsiI PinAI PstI PvuII SacI SapI SmaI SpeI SrfI VspI XhoI Enzymes that do not cut: AatII AflII AhdI AlwNI ApaBI ApaLI AscI AvrII BbsI Bce83I BcgI BclI BfiI BplI BpmI Bpu10I BsaI BsaXI BsbI BseRI BsiWI BsmI BsmBI BspEI BspGI BsrDI BsrGI BssHII BssSI BstEII Bsu36I ClaI DraI DraIII DrdI DrdII EciI Eco47III Eco57I EcoNI FseI FspI HgiEII HpaI MfeI MluI MscI NarI NcoI NgoAIV NruI NspV PacI Pfl1108I PflMI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SanDI ScaI SexAI SfiI SgfI SgrAI SnaBI SphI Sse8387I Sse8647I SspI StuI SwaI Tth111I XbaI XcmI XmnI