(Linear) MAP of: ./Fgr-S_4_F08_T7.seq check: 1319 from: 1 to: 837 Fgr-S_4_F08_T7 837 5 742 ABI Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * July 30, 2001 22:35 .. EagI NotI EcoRV XbaI BsrBI| BsaBI | EcoRI ApaI | XhoI || BstXI | | PstI|Bpu1102I EagI | | | || | | | || | | GAATTGGGCCCTCTAGATGCTGCTCGAGCGGCCGCCAGTGTGATGGATATCTGCAGAATTCGGCTTAGCGTGGTCGCGGCCGAGGTACTCACGGTTGCTT 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 CTTAACCCGGGAGATCTACGACGAGCTCGCCGGCGGTCACACTACCTATAGACGTCTTAAGCCGAATCGCACCAGCGCCGGCTCCATGAGTGCCAACGAA a E L G P L D A A R A A A S V M D I C R I R L S V V A A E V L T V A F - b N W A L * M L L E R P P V * W I S A E F G L A W S R P R Y S R L L - c I G P S R C C S S G R Q C D G Y L Q N S A * R G R G R G T H G C F - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d F Q A R * I S S S R G G T H H I D A S N P K A H D R G L Y E R N S - e N P G R S A A R A A A L T I S I Q L I R S L T T A A S T S V T A - f I P G E L H Q E L P R W H S P Y R C F E A * R P R P R P V * P Q K - BspGI AlwNI PstI | BcgI BcgI | | | | | TTCTCCCTCTTTTCCCTTTTTCTATACCGGATTGTCGCAACCAGATGCTGCAGTCCAGGAGCTAGAGATCCCGAGGATGATGCTACATGGAGTTCGGCTT 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 AAGAGGGAGAAAAGGGAAAAAGATATGGCCTAACAGCGTTGGTCTACGACGTCAGGTCCTCGATCTCTAGGGCTCCTACTACGATGTACCTCAAGCCGAA a L P L F P F S I P D C R N Q M L Q S R S * R S R G * C Y M E F G F - b F S L F S L F L Y R I V A T R C C S P G A R D P E D D A T W S S A S - c S P S F P F F Y T G L S Q P D A A V Q E L E I P R M M L H G V R L - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d K E R K E R K R Y R I T A V L H Q L G P A L S G S S S A V H L E A - e K R G R K G K E I G S Q R L W I S C D L L * L D R P H H * M S N P K - f E G E K G K K * V P N D C G S A A T W S S S I G L I I S C P T R S - AflII BseRI StuI NheI EcoNI | | | | CGAGGAGTAGTTGGGAGGTCCCAGGCAGGAGGCCTTGCCTTTTCGATTGTTGCTACTTTTGTGCTAGCCTTCTTAAGGCAAATCTGTTTAACTTATGTCT 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 GCTCCTCATCAACCCTCCAGGGTCCGTCCTCCGGAACGGAAAAGCTAACAACGATGAAAACACGATCGGAAGAATTCCGTTTAGACAAATTGAATACAGA a E E * L G G P R Q E A L P F R L L L L L C * P S * G K S V * L M S - b R S S W E V P G R R P C L F D C C Y F C A S L L K A N L F N L C L - c R G V V G R S Q A G G L A F S I V A T F V L A F L R Q I C L T Y V C - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d E L L L Q S T G P L L G Q R K S Q Q * K Q A L R R L A F R N L K H R - e S S Y N P P G L C S A K G K R N N S S K H * G E * P L D T * S I D - f R P T T P L D W A P P R A K E I T A V K T S A K K L C I Q K V * T - BglI MfeI XhoI | | | | GTACTCAGACATTGTTGCTTCCGCTGACTCGTCTATGATCGAGCAATTGTATTCGAGCCCTCGAGGCCCCTGGCTTGTATTATGATGCTTGTATGACTTA 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 CATGAGTCTGTAACAACGAAGGCGACTGAGCAGATACTAGCTCGTTAACATAAGCTCGGGAGCTCCGGGGACCGAACATAATACTACGAACATACTGAAT a V L R H C C F R * L V Y D R A I V F E P S R P L A C I M M L V * L I - b Y S D I V A S A D S S M I E Q L Y S S P R G P W L V L * C L Y D L - c T Q T L L L P L T R L * S S N C I R A L E A P G L Y Y D A C M T Y - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d Y E S M T A E A S E D I I S C N Y E L G R P G Q S T N H H K Y S K - e T S L C Q Q K R Q S T * S R A I T N S G E L G R A Q I I I S T H S - f Q V * V N N S G S V R R H D L L Q I R A R S A G P K Y * S A Q I V * - BsbI EcoRV BsaBI | | | TTTATGTTGTAGAGTTGTGTTGTGATATCTTCCCGTGAGTCCCTGATCTTGATCGTACACATTTGCGTGCATGATTAGTGTATGGTCAAATCGGGGGCGT 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 AAATACAACATCTCAACACAACACTATAGAAGGGCACTCAGGGACTAGAACTAGCATGTGTAAACGCACGTACTAATCACATACCAGTTTAGCCCCCGCA a Y V V E L C C D I F P * V P D L D R T H L R A * L V Y G Q I G G V - b F M L * S C V V I S S R E S L I L I V H I C V H D * C M V K S G A S - c L C C R V V L * Y L P V S P * S * S Y T F A C M I S V W S N R G R - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d N I N Y L Q T T I D E R S D R I K I T C M Q T C S * H I T L D P A - e I * T T S N H Q S I K G H T G S R S R V C K R A H N T Y P * I P P T - f K H Q L T T N H Y R G T L G Q D Q D Y V N A H M I L T H D F R P R - BsrDI | CACAATTCCATTAGCAATGACAGGGAAATTACTATCAGAGAAGTGATAAATAAAGGTTGGGGGCTATGTCTTTTAGAAGAACAATTTGTGGAGGATATGA 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 GTGTTAAGGTAATCGTTACTGTCCCTTTAATGATAGTCTCTTCACTATTTATTTCCAACCCCCGATACAGAAAATCTTCTTGTTAAACACCTCCTATACT a T I P L A M T G K L L S E K * * I K V G G Y V F * K N N L W R I * - b Q F H * Q * Q G N Y Y Q R S D K * R L G A M S F R R T I C G G Y E - c H N S I S N D R E I T I R E V I N K G W G L C L L E E Q F V E D M K - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d D C N W * C H C P F * * * L L S L Y L N P A I D K L L V I Q P P Y S - e V I G N A I V P F N S D S F H Y I F T P P * T K * F F L K H L I H - f * L E M L L S L S I V I L S T I F L P Q P S H R K S S C N T S S I - SspI NdeI | | AAATCTCTGGGATAGCCTAAAAAGGAGATGTGAGGAAATAATATTGCATGGAGGGAGGGACAAACCCATATGGATACTGACTGCTAATAGGGAATTTACT 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 TTTAGAGACCCTATCGGATTTTTCCTCTACACTCCTTTATTATAACGTACCTCCCTCCCTGTTTGGGTATACCTATGACTGACGATTATCCCTTAAATGA a K S L G * P K K E M * G N N I A W R E G Q T H M D T D C * * G I Y C - b N L W D S L K R R C E E I I L H G G R D K P I W I L T A N R E F T - c I S G I A * K G D V R K * Y C M E G G T N P Y G Y * L L I G N L L - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d F R Q S L R F L L H S S I I N C P P L S L G M H I S V A L L S N V - e F D R P Y G L F S I H P F L I A H L S P C V W I S V S Q * Y P I * - f F I E P I A * F P S T L F Y Y Q M S P P V F G Y P Y Q S S I P F K S - GCTAAATCTTTATATCTGCATGTAGTTAAAATTGATGTTGGCTTTCCGCANAAATTCCTGTGGAAAGTTAAAGTGCCTGCGAAAAATAAATTTTTTTTCT 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 CGATTTAGAAATATAGACGTACATCAATTTTAACTACAACCGAAAGGCGTNTTTAAGGACACCTTTCAATTTCACGGACGCTTTTTATTTAAAAAAAAGA a * I F I S A C S * N * C W L S A ? I P V E S * S A C E K * I F F L - b A K S L Y L H V V K I D V G F P ? K F L W K V K V P A K N K F F F F - c L N L Y I C M * L K L M L A F R ? N S C G K L K C L R K I N F F S - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d A L D K Y R C T T L I S T P K G C ? N R H F T L T G A F F L N K K - e Q * I K I D A H L * F Q H Q S E A ? I G T S L * L A Q S F Y I K K R - f S F R * I Q M Y N F N I N A K R ? F E Q P F N F H R R F I F K K E - BsmI BseRI | | TTGGCTCTTNTGACAGAGGAGCATTCTTACCAGAGAT 801 ---------+---------+---------+------- 837 AACCGAGAANACTGTCTCCTCGTAAGAATGGTCTCTA a W L ? * Q R S I L T R D - b G S ? D R G A F L P E - c L A L ? T E E H S Y Q R - 801 ---------+---------+---------+------- 837 d K P E ? S L P A N K G S I - e Q S K ? C L L M R V L S - f K A R ? V S S C E * W L - Enzymes that do cut: AflII AlwNI ApaI BcgI BglI Bpu1102I BsaBI BsbI BseRI BsmI BspGI BsrBI BsrDI BstXI EagI EcoNI EcoRI EcoRV MfeI NdeI NheI NotI PstI SspI StuI XbaI XhoI Enzymes that do not cut: AatII AceIII AhdI ApaBI ApaLI AscI AvrII BamHI BbsI Bce83I BclI BfiI BglII BplI BpmI Bpu10I BsaI BsaXI BsgI BsiWI BsmBI BspEI BspLU11I BspMI BsrGI BssHII BssSI Bst1107I BstEII Bsu36I ClaI DraI DraIII DrdI DrdII EarI EciI Eco47III Eco57I FseI FspI HgiEII HindIII HpaI KpnI MluI MscI NarI NcoI NgoAIV NruI NsiI NspV PacI Pfl1108I PflMI PinAI PmeI PmlI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SanDI SapI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI Sse8387I Sse8647I SwaI Tth111I VspI XcmI XmnI