(Linear) MAP of: ./Fgr-S_7_H03_T7.seq check: 944 from: 1 to: 1064 Fgr-S_7_H03_T7 1064 34 289 ABI Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * July 30, 2001 22:35 .. EagI NotI EcoRV BsrBI| BsaBI | EcoRI NspV XbaI XhoI || BstXI | | PstI|Bpu1102I EagI BplI | | | || | | | || | | | NGGATTCGAATTGGGCCTCTAGAGGCTGCTCGAGCGGCCGCCAGTGTGATGGATATCTGCAGAATTCGGCTTAGCGTGGTCGCGGCCGAGGTACTCTCGT 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 NCCTAAGCTTAACCCGGAGATCTCCGACGAGCTCGCCGGCGGTCACACTACCTATAGACGTCTTAAGCCGAATCGCACCAGCGCCGGCTCCATGAGAGCA a ? I R I G P L E A A R A A A S V M D I C R I R L S V V A A E V L S Y - b G F E L G L * R L L E R P P V * W I S A E F G L A W S R P R Y S R - c D S N W A S R G C S S G R Q C D G Y L Q N S A * R G R G R G T L V - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d I R I P G R S A A R A A A L T I S I Q L I R S L T T A A S T S E - e S E F Q A E L P Q E L P R W H S P Y R C F E A * R P R P R P V R T - f P N S N P R * L S S S R G G T H H I D A S N P K A H D R G L Y E R - VspI BfiI | | ATTAATCACATGAACTGGGAAGAAACCGCATCCCCATCAAAGCGCATCAGCCTGTCCAAAGGAGTAGGCTAGTCACCAAACTTCGGCAGCGAAATCATGC 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 TAATTAGTGTACTTGACCCTTCTTTGGCGTAGGGGTAGTTTCGCGTAGTCGGACAGGTTTCCTCATCCGATCAGTGGTTTGAAGCCGTCGCTTTAGTACG a * S H E L G R N R I P I K A H Q P V Q R S R L V T K L R Q R N H A - b I N H M N W E E T A S P S K R I S L S K G V G * S P N F G S E I M P - c L I T * T G K K P H P H Q S A S A C P K E * A S H Q T S A A K S C - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d Y * D C S S P L F R M G M L A C * G T W L L L S T V L S R C R F * A - e N I V H V P F F G C G W * L A D A Q G F S Y A L * W V E A A F D H - f I L * M F Q S S V A D G D F R M L R D L P T P * D G F K P L S I M - BspMI SmaI | Eco57I BspMI SrfI | | | | | CCGCCCAACAACATCCCATGTAAATAGGAGAGTGAGAAGTTGCTTCAGCATGGTGTATAGATCCTTCACGAAACCAAAATAAAGCCCCGACCTGCCCGGG 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 GGCGGGTTGTTGTAGGGTACATTTATCCTCTCACTCTTCAACGAAGTCGTACCACATATCTAGGAAGTGCTTTGGTTTTATTTCGGGGCTGGACGGGCCC a R P T T S H V N R R V R S C F S M V Y R S F T K P K * S P D L P G - b A Q Q H P M * I G E * E V A S A W C I D P S R N Q N K A P T C P G - c P P N N I P C K * E S E K L L Q H G V * I L H E T K I K P R P A R A - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d R G V V D W T F L L T L L Q K L M T Y L D K V F G F Y L G S R G P - e G G L L M G H L Y S L S F N S * C P T Y I R * S V L I F G R G A R - f G A W C C G M Y I P S H S T A E A H H I S G E R F W F L A G V Q G P - DraIII PmlI | Eco57I | | BspMI | | | BstEII BssHII EarI PstI | | | | | | | | | | | | CAGGTGACCTACAACTGAGATGACGGTCTGAGTCGCTGCGCGCGGACAACCCAAAAATAGCTTGAAACCTCTTCTTAGTAAACCTGCAGAGAACACGTGG 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 GTCCACTGGATGTTGACTCTACTGCCAGACTCAGCGACGCGCGCCTGTTGGGTTTTTATCGAACTTTGGAGAAGAATCATTTGGACGTCTCTTGTGCACC a Q V T Y N * D D G L S R C A R T T Q K * L E T S S * * T C R E H V V - b R * P T T E M T V * V A A R G Q P K N S L K P L L S K P A E N T W - c G D L Q L R * R S E S L R A D N P K I A * N L F L V N L Q R T R G - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d C T V * L Q S S P R L R Q A R V V W F Y S S V E E * Y V Q L S C T - e A P S R C S L H R D S D S R A S L G F I A Q F R K K T F R C L V R P - f L H G V V S I V T Q T A A R P C G L F L K F G R R L L G A S F V H - BsbI EciI MluI XcmI| BssSI Pfl1108I | | || | | TGATTATTCTTCAGGTGATAGGGCGGAGATGAATGAGGGCGACGTGCGTACCACGCGTGTTGTGGGGCACGAGTGATGGGAAGTCGTAGATAATGGGGTC 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 ACTAATAAGAAGTCCACTATCCCGCCTCTACTTACTCCCGCTGCACGCATGGTGCGCACAACACCCCGTGCTCACTACCCTTCAGCATCTATTACCCCAG a I I L Q V I G R R * M R A T C V P R V L W G T S D G K S * I M G S - b * L F F R * * G G D E * G R R A Y H A C C G A R V M G S R R * W G L - c D Y S S G D R A E M N E G D V R T T R V V G H E * W E V V D N G V - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d T I I R * T I P R L H I L A V H T G R T N H P V L S P F D Y I I P D - e S * E E P S L A S I F S P S T R V V R T T P C S H H S T T S L P T - f H N N K L H Y P P S S H P R R A Y W A H Q P A R T I P L R L Y H P - NarI BglI | BpmI BseRI XcmI BfiI | | | | | | | | TGGAGGTGAGGAAGTAAGGGGAGGAGTAGACGCGCTGGCGGCCAGCGTACATCTGGCCCCAGTGGCGCCGAGGGCAACCGCCACTGCCGCCGTTGGGTGC 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 ACCTCCACTCCTTCATTCCCCTCCTCATCTGCGCGACCGCCGGTCGCATGTAGACCGGGGTCACCGCGGCTCCCGTTGGCGGTGACGGCGGCAACCCACG a G G E E V R G G V D A L A A S V H L A P V A P R A T A T A A V G C - b E V R K * G E E * T R W R P A Y I W P Q W R R G Q P P L P P L G A - c W R * G S K G R S R R A G G Q R T S G P S G A E G N R H C R R W V R - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d P P S S T L P P T S A S A A L T C R A G T A G L A V A V A A T P H - e Q L H P L L P L L L R A P P W R V D P G L P A S P L R W Q R R Q T - f R S T L F Y P S S Y V R Q R G A Y M Q G W H R R P C G G S G G N P A - FspI BseRI | | GACGATGATAGGTGCATCCTCCGGCGGCAAGCGTAGAGTCCCCGCAACATGCGCACTTGCACGCATAGACGAGGAGGAACTGGTAGCGCCAGACAAATAC 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 CTGCTACTATCCACGTAGGAGGCCGCCGTTCGCATCTCAGGGGCGTTGTACGCGTGAACGTGCGTATCTGCTCCTCCTTGACCATCGCGGTCTGTTTATG a D D D R C I L R R Q A * S P R N M R T C T H R R G G T G S A R Q I P - b T M I G A S S G G K R R V P A T C A L A R I D E E E L V A P D K Y - c R * * V H P P A A S V E S P Q H A H L H A * T R R N W * R Q T N T - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d S S S L H M R R R C A Y L G R L M R V Q V C L R P P V P L A L C I - e R R H Y T C G G A A L T S D G C C A C K C A Y V L L F Q Y R W V F V - f V I I P A D E P P L R L T G A V H A S A R M S S S S S T A G S L Y - ApaI BcgI| SmaI || BcgI BsrBI BseRI | || | | | CACGCCCCGGGCCCTCCCTCCCCGAAACTGATGCTTGACGTGTGGAGACAGGGAGTGACGGAGACAGCGAGCGGAGCCGCTGACATCTCTTGTACGAGGC 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 GTGCGGGGCCCGGGAGGGAGGGGCTTTGACTACGAACTGCACACCTCTGTCCCTCACTGCCTCTGTCGCTCGCCTCGGCGACTGTAGAGAACATGCTCCG a R P G P S L P E T D A * R V E T G S D G D S E R S R * H L L Y E A - b H A P G P P S P K L M L D V W R Q G V T E T A S G A A D I S C T R Q - c T P R A L P P R N * C L T C G D R E * R R Q R A E P L T S L V R G - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d G R G P G E R G S V S A Q R T S V P L S P S L S R L R Q C R K Y S A - e V G R A R G G R F Q H K V H P S L S H R L C R A S G S V D R T R P - f W A G P G G E G F S I S S T H L C P T V S V A L P A A S M E Q V L - SmaI | AGTCGCGCTCCTCCCGGCCCCCTGGCACCCCGAGCCCCGCTACCCCGCACCTCCCGGGGAGAGTAATCCGAGCTACAGAGTGTACTTAGTAACGTAGTGG 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 TCAGCGCGAGGAGGGCCGGGGGACCGTGGGGCTCGGGGCGATGGGGCGTGGAGGGCCCCTCTCATTAGGCTCGATGTCTCACATGAATCATTGCATCACC a V A L L P A P W H P E P R Y P A P P G E S N P S Y R V Y L V T * W - b S R S S R P P G T P S P A T P H L P G R V I R A T E C T * * R S G - c S R A P P G P L A P R A P L P R T S R G E * S E L Q S V L S N V V A - 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 d T A S R G A G Q C G S G R * G A G G P S L L G L * L T Y K T V Y H - e L R A G G P G R A G R A G S G R V E R P S Y D S S C L T S L L T T - f C D R E E R G G P V G L G A V G C R G P L T I R A V S H V * Y R L P - BseRI ApaLI | BsbI AhdI | | | | CTCCACACACTCGCGGCGTGCACGACACTACTCCTCGCAGCGGCAGCCATTATAATGCGGGTGNGCGTAGTGTTGCACAGTGTCAGTGCTAACGACACAC 901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1000 GAGGTGTGTGAGCGCCGCACGTGCTGTGATGAGGAGCGTCGCCGTCGGTAATATTACGCCCACNCGCATCACAACGTGTCACAGTCACGATTGCTGTGTG a L H T L A A C T T L L L A A A A I I M R V ? V V L H S V S A N D T R - b S T H S R R A R H Y S S Q R Q P L * C G ? A * C C T V S V L T T H - c P H T R G V H D T T P R S G S H Y N A G ? R S V A Q C Q C * R H T - 901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1000 d S W V S A A H V V S S R A A A A M I I R T ? T T N C L T L A L S V - e A G C V R P T C S V V G R L P L W * L A P ? R L T A C H * H * R C V - f E V C E R R A R C * E E C R C G N Y H P H A Y H Q V T D T S V V C - AhdI BsaI AatII| | || GGTCTCTGCGCTGACGTCCGGTCTAGCGAACGCCGTGTGGATGCCAAGGTACAAGCTAAGAGCG 1001 ---------+---------+---------+---------+---------+---------+---- 1064 CCAGAGACGCGACTGCAGGCCAGATCGCTTGCGGCACACCTACGGTTCCATGTTCGATTCTCGC a S L R * R P V * R T P C G C Q G T S * E - b G L C A D V R S S E R R V D A K V Q A K S - c V S A L T S G L A N A V W M P R Y K L R A - 1001 ---------+---------+---------+---------+---------+---------+---- 1064 d R D R R Q R G T * R V G H P H W P V L * S R - e T E A S V D P R A F A T H I G L Y L S L A - f P R Q A S T R D L S R R T S A L T C A L L - Enzymes that do cut: AatII AhdI ApaI ApaLI BcgI BfiI BglI BplI BpmI Bpu1102I BsaI BsaBI BsbI BseRI BspMI BsrBI BssHII BssSI BstEII BstXI DraIII EagI EarI EciI Eco57I EcoRI EcoRV FspI MluI NarI NotI NspV Pfl1108I PmlI PstI SmaI SrfI VspI XbaI XcmI XhoI Enzymes that do not cut: AceIII AflII AlwNI ApaBI AscI AvrII BamHI BbsI Bce83I BclI BglII Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BspEI BspGI BspLU11I BsrDI BsrGI Bst1107I Bsu36I ClaI DraI DrdI DrdII Eco47III EcoNI FseI HgiEII HindIII HpaI KpnI MfeI MscI NcoI NdeI NgoAIV NheI NruI NsiI PacI PflMI PinAI PmeI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SanDI SapI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI Sse8387I Sse8647I SspI StuI SwaI Tth111I XmnI