(Linear) MAP of: ./Fgr-S_9_B11_T7.seq check: 135 from: 1 to: 883 Fgr-S_9_B11_T7 883 22 702 ABI Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * July 30, 2001 22:34 .. EagI NotI EcoRV BsrBI| BsaBI | EcoRI ApaI XhoI || BstXI | | PstI|Bpu1102I EagI KpnI SalI | | || | | | || | | | | CGATATGGGCCCTCTAGTATGCTGCTCGAGCGGCCGCCAGTGTGATGGATATCTGCAGAATTCGGCTTAGCGTGGTCGCGGCCGAGGTACCGCGCCGTCG 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 GCTATACCCGGGAGATCATACGACGAGCTCGCCGGCGGTCACACTACCTATAGACGTCTTAAGCCGAATCGCACCAGCGCCGGCTCCATGGCGCGGCAGC a R Y G P S S M L L E R P P V * W I S A E F G L A W S R P R Y R A V D - b D M G P L V C C S S G R Q C D G Y L Q N S A * R G R G R G T A P S - c I W A L * Y A A R A A A S V M D I C R I R L S V V A A E V P R R R - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d I H A R * Y A A R A A A L T I S I Q L I R S L T T A A S T G R R R - e S I P G R T H Q E L P R W H S P Y R C F E A * R P R P R P V A G D - f Y P G E L I S S S R G G T H H I D A S N P K A H D R G L Y R A T - HindIII | ACAGCAGGGGGCGCACCGTCGATTTTTACCTCTCGGCACGGCGCAACAGTAAAGCCGCATACCGCTTTATGGGAAAGCTTCTGAGTAAGGCGAAGCGCGG 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 TGTCGTCCCCCGCGTGGCAGCTAAAAATGGAGAGCCGTGCCGCGTTGTCATTTCGGCGTATGGCGAAATACCCTTTCGAAGACTCATTCCGCTTCGCGCC a S R G R T V D F Y L S A R R N S K A A Y R F M G K L L S K A K R G - b T A G G A P S I F T S R H G A T V K P H T A L W E S F * V R R S A G - c Q Q G A H R R F L P L G T A Q Q * S R I P L Y G K A S E * G E A R - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d C C P A C R R N K G R P V A C C Y L R M G S * P F A E S Y P S A R - e V A P P A G D I K V E R C P A V T F G C V A K H S L K Q T L R L A - f S L L P R V T S K * R E A R R L L L A A Y R K I P F S R L L A F R P - VspI ApaBI | | GCAAATTCCCCGGCTGATTAATACCGATAAAGCCCCAACATACGCGCGTGCGCTGGCGTTACTAAAGCGCGAAGGAAAATGTCCGCAGAATGTGCATCAC 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 CGTTTAAGGGGCCGACTAATTATGGCTATTTCGGGGTTGTATGCGCGCACGCGACCGCAATGATTTCGCGCTTCCTTTTACAGGCGTCTTACACGTAGTG a Q I P R L I N T D K A P T Y A R A L A L L K R E G K C P Q N V H H - b K F P G * L I P I K P Q H T R V R W R Y * S A K E N V R R M C I T - c A N S P A D * Y R * S P N I R A C A G V T K A R R K M S A E C A S P - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d A F E G A S * Y R Y L G L M R A H A P T V L A R L F I D A S H A D - e P L N G P Q N I G I F G W C V R T R Q R * * L A F S F T R L I H M V - f C I G R S I L V S L A G V Y A R A S A N S F R S P F H G C F T C * - BspMI EagI | NotI | BglI| | BspMI|| | BglI SmaI ||| | SmaI SrfI ||| | SrfI BsrGI | ||| | | | CGGCAGATAAAGTACCTGCCCGGGCGGCCGCCCGGGCAGGTGTACACACTCTCACAAGCACAACCCCTAACTTCTTACCTTTTTTTTGTTTGTAATAAAG 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 GCCGTCTATTTCATGGACGGGCCCGCCGGCGGGCCCGTCCACATGTGTGAGAGTGTTCGTGTTGGGGATTGAAGAATGGAAAAAAAACAAACATTATTTC a R Q I K Y L P G R P P G Q V Y T L S Q A Q P L T S Y L F F V C N K E - b G R * S T C P G G R P G R C T H S H K H N P * L L T F F L F V I K - c A D K V P A R A A A R A G V H T L T S T T P N F L P F F C L * * R - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d G A S L T G A R A A A R A P T C V R V L V V G L K K G K K Q K Y Y L - e P L Y L V Q G P P R G P L H V C E * L C L G * S R V K K K N T I F - f R C I F Y R G P R G G P C T Y V S E C A C G R V E * R K K T Q L L - ScaI BseRI | | AGGCAAGCGGCACAACAAAGCATAAGTACTTATTAGTCTATATATAAATATGTATATATCGGCCCTTCACTACAACAAGCGGCTATCTCCTCACACGGGA 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 TCCGTTCGCCGTGTTGTTTCGTATTCATGAATAATCAGATATATATTTATACATATATAGCCGGGAAGTGATGTTGTTCGCCGATAGAGGAGTGTGCCCT a A S G T T K H K Y L L V Y I * I C I Y R P F T T T S G Y L L T R E - b R Q A A Q Q S I S T Y * S I Y K Y V Y I G P S L Q Q A A I S S H G N - c G K R H N K A * V L I S L Y I N M Y I S A L H Y N K R L S P H T G - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d P L R C L L A Y T S I L R Y I F I Y I D A R * * L L R S D G * V P - e L C A A C C L M L V * * D I Y L Y T Y I P G E S C C A A I E E C P - f S A L P V V F C L Y K N T * I Y I H I Y R G K V V V L P * R R V R S - Eco57I BsrBI BcgI BseRI BsmI | Eco57I EarI | NcoI | BsrBI| | | | | | | | || ACCCTGAAGATGGATGAATGCTCCATGCACCAAAAACCTCTTTCCTCTCTTCAGGGAGCGGCGACGTTCTCCATGGCGAGCGGCGCAATCTCCTCGCCGA 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 TGGGACTTCTACCTACTTACGAGGTACGTGGTTTTTGGAGAAAGGAGAGAAGTCCCTCGCCGCTGCAAGAGGTACCGCTCGCCGCGTTAGAGGAGCGGCT a P * R W M N A P C T K N L F P L F R E R R R S P W R A A Q S P R R - b P E D G * M L H A P K T S F L S S G S G D V L H G E R R N L L A D - c T L K M D E C S M H Q K P L S S L Q G A A T F S M A S G A I S S P M - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d V R F I S S H E M C W F G R E E R * P A A V N E M A L P A I E E G - e F G S S P H I S W A G F V E K R E E P L P S T R W P S R R L R R A S - f G Q L H I F A G H V L F R K G R K L S R R R E G H R A A C D G R R - XcmI PflMI| BcgI BfiI BfiI || | | | || TGACCCCTGGCTCGCTCCCCTCGACCCCCAGTTCCAGAAGCACGTCCTCCCAGTTCTTGGCTGGCCCCTTCCAGGAGAGATCCTGGATCATGCAGTTCTT 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 ACTGGGGACCGAGCGAGGGGAGCTGGGGGTCAAGGTCTTCGTGCAGGAGGGTCAAGAACCGACCGGGGAAGGTCCTCTCTAGGACCTAGTACGTCAAGAA a * P L A R S P R P P V P E A R P P S S W L A P S R R D P G S C S S * - b D P W L A P L D P Q F Q K H V L P V L G W P L P G E I L D H A V L - c T P G S L P S T P S S R S T S S Q F L A G P F Q E R S W I M Q F L - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d I V G P E S G E V G L E L L V D E W N K A P G K W S L D Q I M C N K - e S G Q S A G R S G W N W F C T R G T R P Q G R G P S I R S * A T R - f H G R A R E G R G G T G S A R G G L E Q S A G E L L S G P D H L E - PflMI DraI | | GACCATCTCATGGTAGGCTGGCGTGCCGCACNCTTTAAACGGGNNNNNNNNNANAGGNNNNNNNNNNTNNTGGGGGGNTTTTNGGTTTNTTGGGTTAGTG 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 CTGGTAGAGTACCATCCGACCGCACGGCGTGNGAAATTTGCCCNNNNNNNNNTNTCCNNNNNNNNNNANNACCCCCCNAAAANCCAAANAACCCAATCAC a P S H G R L A C R T L * T G ? ? ? ? ? ? ? ? ? G G ? ? G ? L G * W - b D H L M V G W R A A ? F K R ? ? ? ? R ? ? ? ? ? G G F ? V ? W V S G - c T I S W * A G V P H ? L N G ? ? ? ? G ? ? ? ? W G ? F ? F ? G L V - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d V M E H Y A P T G C ? K F P ? ? ? ? P ? ? ? ? ? P ? K ? N ? P N T - e S W R M T P Q R A A ? K L R ? ? ? ? L ? ? ? ? ? P P ? ? T ? Q T L - f Q G D * P L S A H R V ? * V P ? ? ? ? ? ? ? ? ? P P ? K P K ? P * H - BsbI | GNTTTGNTTGGGGTGGGGGGGTTTGGGTGGTGGGGGGGTGGTTTTNGGTTGTTGAAGGGGGGGNNNTNGTGGTGTTGNTNNAT 801 ---------+---------+---------+---------+---------+---------+---------+---------+--- 883 CNAAACNAACCCCACCCCCCCAAACCCACCACCCCCCCACCAAAANCCAACAACTTCCCCCCCNNNANCACCACAACNANNTA a ? ? L G W G G L G G G G V V ? G C * R G G ? ? G V ? ? - b F ? W G G G V W V V G G W F ? V V E G G ? ? V V L ? ? - c ? L ? G V G G F G W W G G G F ? L L K G G ? ? W C ? ? - 801 ---------+---------+---------+---------+---------+---------+---------+---------+--- 883 d ? K ? P T P P N P H H P P P K ? N N F P P ? ? H H Q ? ? - e P ? ? Q P P P T Q T T P P H N ? T T S P P ? ? T T N ? ? - f ? Q ? P H P P K P P P P T T K P Q Q L P P ? ? P T ? ? - Enzymes that do cut: ApaI ApaBI BcgI BfiI BglI Bpu1102I BsaBI BsbI BseRI BsmI BspMI BsrBI BsrGI BstXI DraI EagI EarI Eco57I EcoRI EcoRV HindIII KpnI NcoI NotI PflMI PstI SalI ScaI SmaI SrfI VspI XcmI XhoI Enzymes that do not cut: AatII AceIII AflII AhdI AlwNI ApaLI AscI AvrII BamHI BbsI Bce83I BclI BglII BplI BpmI Bpu10I BsaI BsaXI BsgI BsiWI BsmBI BspEI BspGI BspLU11I BsrDI BssHII BssSI Bst1107I BstEII Bsu36I ClaI DraIII DrdI DrdII EciI Eco47III EcoNI FseI FspI HgiEII HpaI MfeI MluI MscI NarI NdeI NgoAIV NheI NruI NsiI NspV PacI Pfl1108I PinAI PmeI PmlI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SanDI SapI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI Sse8387I Sse8647I SspI StuI SwaI Tth111I XbaI XmnI