(Linear) MAP of: ./Fgr-S_9_F02_T7.seq check: 5289 from: 1 to: 907 Fgr-S_9_F02_T7 907 11 603 ABI Using Enzyme data from: /home/pmiguel/dat/enzyme.dat FileCheck: 3232 Restriction enzyme data file used by the programs MAP, MAPSORT and MAPPLOT. A complete format description of this file may be found in the Data Files manual. Only one enzyme per recognition specificity is used when the '*' is preSsed to select all default enzymes. In addition, enzymes that are commercially available but are not unique in . . . With 115 enzymes: * July 30, 2001 22:34 .. EagI EcoRV XbaI XhoI NotI BsaBI | EcoRI NspV ApaI | SphI|BsrBI| BstXI | | PstI|Bpu1102I EagI | | | || || | | | || | | NGATTCGAATTGGGCCCTCTAGAGCATGCTCGAGCGGCCGCCAGTGTGATGGATATCTGCAGAATTCGGCTTAGCGTGGTCGCGGCCGAGGTTTTTTTTT 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 NCTAAGCTTAACCCGGGAGATCTCGTACGAGCTCGCCGGCGGTCACACTACCTATAGACGTCTTAAGCCGAATCGCACCAGCGCCGGCTCCAAAAAAAAA a ? F E L G P L E H A R A A A S V M D I C R I R L S V V A A E V F F F - b D S N W A L * S M L E R P P V * W I S A E F G L A W S R P R F F F - c I R I G P S R A C S S G R Q C D G Y L Q N S A * R G R G R G F F F - 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 d I R I P G E L A H E L P R W H S P Y R C F E A * R P R P R P K K K - e S E F Q A R * L M S S R G G T H H I D A S N P K A H D R G L N K K - f N S N P G R S C A R A A A L T I S I Q L I R S L T T A A S T K K - DraI ApaBI | | TTTTTTTTTTAAAATCCAAGGACACTCATAGGATAAGATTTGATAGCAGATCATGCAAATAAGTATATCAGGTATCAAACAAGATGTCCGAGAAGGCCAA 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 AAAAAAAAAATTTTAGGTTCCTGTGAGTATCCTATTCTAAACTATCGTCTAGTACGTTTATTCATATAGTCCATAGTTTGTTCTACAGGCTCTTCCGGTT a F F L K S K D T H R I R F D S R S C K * V Y Q V S N K M S E K A K - b F F F * N P R T L I G * D L I A D H A N K Y I R Y Q T R C P R R P K - c F F F K I Q G H S * D K I * * Q I M Q I S I S G I K Q D V R E G Q - 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 d K K K L I W P C E Y S L I Q Y C I M C I L I D P I L C S T R S P W - e K K K * F G L V S M P Y S K I A S * A F L Y I L Y * V L H G L L G - f K K K K F D L S V * L I L N S L L D H L Y T Y * T D F L I D S F A L - AflII BseRI BsaXI EciI | | | | AACAGAAATTCAGAAACTAGCACTTGATAACAACAGAACCCACTAGCTGCCTTAAGCATCTCATCACTTCTTCTCCTCCTTCTCCGCCTCGGCAGCCTTC 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 TTGTCTTTAAGTCTTTGATCGTGAACTATTGTTGTCTTGGGTGATCGACGGAATTCGTAGAGTAGTGAAGAAGAGGAGGAAGAGGCGGAGCCGTCGGAAG a T E I Q K L A L D N N R T H * L P * A S H H F F S S F S A S A A F - b Q K F R N * H L I T T E P T S C L K H L I T S S P P S P P R Q P S - c N R N S E T S T * * Q Q N P L A A L S I S S L L L L L L R L G S L L - 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 d F L F E S V L V Q Y C C F G S A A K L M E D S R R R R R R R P L R - e F C F N L F * C K I V V S G V L Q R L C R M V E E G G E G G R C G E - f V S I * F S A S S L L L V W * S G * A D * * K K E E K E A E A A K - EagI NotI BglI| BspMI|| SmaI ||| DrdII Bce83I SrfI |||BsrBI EcoRI BstXI EagI SpeI BamHI | | | ||| | | | | | | TTCTGCCTGGCTCCCAACTGGCGCTGGTTCATCCTCTCGACACCTGCCCGGGCGGCCGCTCAAGCCGAATTCCAGCACACTGGCGGCCGTTACTAGTGGA 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 AAGACGGACCGAGGGTTGACCGCGACCAAGTAGGAGAGCTGTGGACGGGCCCGCCGGCGAGTTCGGCTTAAGGTCGTGTGACCGCCGGCAATGATCACCT a F C L A P N W R W F I L S T P A R A A A Q A E F Q H T G G R Y * W I - b S A W L P T G A G S S S R H L P G R P L K P N S S T L A A V T S G - c L P G S Q L A L V H P L D T C P G G R S S R I P A H W R P L L V D - 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 d R R G P E W S A S T * G R S V Q G P P R E L R I G A C Q R G N S T S - e E A Q S G V P A P E D E R C R G P R G S L G F E L V S A A T V L P - f K Q R A G L Q R Q N M R E V G A R A A A * A S N W C V P P R * * H - HindIII SacI KpnI | BsrBI | | | | TCCGAGCTCGGTACCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGC 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 AGGCTCGAGCCATGGTTCGAACCGCATTAGTACCAGTATCGACAAAGGACACACTTTAACAATAGGCGAGTGTTAAGGTGTGTTGTATGCTCGGCCTTCG a R A R Y Q A W R N H G H S C F L C E I V I R S Q F H T T Y E P E A - b S E L G T K L G V I M V I A V S C V K L L S A H N S T Q H T S R K H - c P S S V P S L A * S W S * L F P V * N C Y P L T I P H N I R A G S - 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 d G L E T G L K A Y D H D Y S N G T H F Q * G S V I G C L M R A P L - e D S S P V L S P T I M T M A T E Q T F N N D A * L E V C C V L R F - f I R A R Y W A Q R L * P * L Q K R H S I T I R E C N W V V Y S G S A - VspI PvuII | | ATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGC 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 TATTTCACATTTCGGACCCCACGGATTACTCACTCGATTGAGTGTAATTAACGCAACGCGAGTGACGGGCGAAAGGTCAGCCCTTTGGACAGCACGGTCG a * S V K P G V P N E * A N S H * L R C A H C P L S S R E T C R A S - b K V * S L G C L M S E L T H I N C V A L T A R F P V G K P V V P A - c I K C K A W G A * * V S * L T L I A L R S L P A F Q S G N L S C Q L - 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 d M F H L A Q P A * H T L * S V N I A N R E S G A K W D P F R D H W - e C L T Y L R P H R I L S S V * M L Q T A S V A R K G T P F G T T G A - f Y L T F G P T G L S H A L E C * N R Q A * Q G S E L R S V Q R A L - EarI VspI BsaXI SapI | | | TGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCT 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 ACGTAATTACTTAGCCGGTTGCGCGCCCCTCTCCGCCAAACGCATAACCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGA a C I N E S A N A R G E A V C V L G A L P L P R S L T R C A R S F G C - b A L M N R P T R G E R R F A Y W A L F R F L A H * L A A L G R S A - c H * * I G Q R A G R G G L R I G R S S A S S L T D S L R S V V R L - 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 d S C * H I P W R A P L P P K R I P R E E A E E S V S E S R E T T R S - e A N I F R G V R P S L R N A Y Q A S K R K R A * Q S A A S P R E A - f Q M L S D A L A R P S A T Q T N P A R G S G R E S V R Q A R D N P - BsrBI AceIII BspLU11I | | | GCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATTCACAGAATCAGGGGATAACGCAGGGAAGAACATGTGAGCAAAAAGGCACCAAAAG 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 CGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAAGTGTCTTAGTCCCCTATTGCGTCCCTTCTTGTACACTCGTTTTTCCGTGGTTTTC a G E R Y Q L T Q R R * Y G Y S Q N Q G I T Q G R T C E Q K G T K R - b A A S G I S S L K G G N T V I H R I R G * R R E E H V S K K A P K G - c R R A V S A H S K A V I R L F T E S G D N A G K N M * A K R H Q K - 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 d R R A T D A * E F A T I R N N V S D P S L A P F F M H A F L C W F - e A A L P I L E S L P P L V T I * L I L P Y R L S S C T L L F A G F - f Q P S R Y * S V * L R Y Y P * E C F * P I V C P L V H S C F P V L L - GCCCAGGAACCGTAAAAAGGCCGGGTTGGTTGGCGTTTTTCCATAAGCTCCGGCCCCCTGACAAGCATTACAAAAATCGACGCTCAAATCAGAAGGGGGC 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 CGGGTCCTTGGCATTTTTCCGGCCCAACCAACCGCAAAAAGGTATTCGAGGCCGGGGGACTGTTCGTAATGTTTTTAGCTGCGAGTTTAGTCTTCCCCCG a P R N R K K A G L V G V F P * A P A P * Q A L Q K S T L K S E G G - b P G T V K R P G W L A F F H K L R P P D K H Y K N R R S N Q K G A - c A Q E P * K G R V G W R F S I S S G P L T S I T K I D A Q I R R G R - 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 d A W S G Y F P R T P Q R K E M L E P G R V L M V F I S A * I L L P - e P G P V T F L G P Q N A N K W L S R G G S L C * L F R R E F * F P A - f G L F R L F A P N T P T K G Y A G A G Q C A N C F D V S L D S P P - GAAACCC 901 ------- 907 CTTTGGG a E T - b K P - c N - 901 ------- 907 d R F G - e F G - f S V - Enzymes that do cut: AceIII AflII ApaI ApaBI BamHI Bce83I BglI Bpu1102I BsaBI BsaXI BseRI BspLU11I BspMI BsrBI BstXI DraI DrdII EagI EarI EciI EcoRI EcoRV HindIII KpnI NotI NspV PstI PvuII SacI SapI SmaI SpeI SphI SrfI VspI XbaI XhoI Enzymes that do not cut: AatII AhdI AlwNI ApaLI AscI AvrII BbsI BcgI BclI BfiI BglII BplI BpmI Bpu10I BsaI BsbI BsgI BsiWI BsmI BsmBI BspEI BspGI BsrDI BsrGI BssHII BssSI Bst1107I BstEII Bsu36I ClaI DraIII DrdI Eco47III Eco57I EcoNI FseI FspI HgiEII HpaI MfeI MluI MscI NarI NcoI NdeI NgoAIV NheI NruI NsiI PacI Pfl1108I PflMI PinAI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SanDI ScaI SexAI SfiI SgfI SgrAI SnaBI Sse8387I Sse8647I SspI StuI SwaI Tth111I XcmI XmnI