BLASTX 2.1.3 [Apr-1-2001]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query=
/phillip/projects/Xu/Fgr-S/seq_dir/blast_NcrP/Fgr-S_1_D04_T7.seq
(345 letters)
Database: /phillip/Ncr/Ncr_P
6531 sequences; 3,158,374 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
NCU07175.1 NCU07175.1 hypothetical protein (85579 - 87606) 27 1.8
NCU07496.1 NCU07496.1 hypothetical protein (67709 - 74407) 26 3.0
NCU01546.1 NCU01546.1 hypothetical protein (28347 - 31533) 26 4.0
NCU09362.1 NCU09362.1 predicted protein (5900 - 8962) 26 4.0
NCU09333.1 NCU09333.1 hypothetical protein (31089 - 28765) 25 5.2
NCU08154.1 NCU08154.1 predicted protein (40649 - 42112) 25 5.2
NCU10007.1 NCU10007.1 MALATE SYNTHASE, GLYOXYSOMAL (19263 - 17567) 25 5.2
NCU08132.1 NCU08132.1 hypothetical protein (37900 - 45290) 25 8.8
NCU00046.1 NCU00046.1 hypothetical protein (34261 - 31619) 25 8.8
NCU00695.1 NCU00695.1 predicted protein (424527 - 426137) 25 8.8
NCU00691.1 NCU00691.1 hypothetical protein (411532 - 414017) 25 8.8
NCU06684.1 NCU06684.1 hypothetical protein (17333 - 19505) 25 8.8
NCU08896.1 NCU08896.1 hypothetical protein (57532 - 53806) 25 8.8
NCU09758.1 NCU09758.1 hypothetical protein (15938 - 12305) 25 8.8
>NCU07175.1 NCU07175.1 hypothetical protein (85579 - 87606)
Length = 589
Score = 26.9 bits (58), Expect = 1.8
Identities = 16/36 (44%), Positives = 19/36 (52%), Gaps = 2/36 (5%)
Frame = -1
Query: 138 THDMATKKSPWTGERSETSHA--HGSNGEDEASELR 37
THD + P + + SHA HGSNG DE E R
Sbjct: 3 THDSISSVQP---DLEQPSHAGGHGSNGIDELDESR 35
>NCU07496.1 NCU07496.1 hypothetical protein (67709 - 74407)
Length = 2140
Score = 26.2 bits (56), Expect = 3.0
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = -1
Query: 120 KKSPWTGERSETSHAHGSNGEDEA 49
KK+P E E HGS GED+A
Sbjct: 1289 KKTPSPEEGEEEGEDHGSAGEDDA 1312
>NCU01546.1 NCU01546.1 hypothetical protein (28347 - 31533)
Length = 870
Score = 25.8 bits (55), Expect = 4.0
Identities = 12/33 (36%), Positives = 16/33 (48%)
Frame = -1
Query: 102 GERSETSHAHGSNGEDEASELRRWAHHGPRPRP 4
G+ + +H H S +E S H PRPRP
Sbjct: 670 GKAKKKNHHHKSLLVEEESTTEAKVEHHPRPRP 702
>NCU09362.1 NCU09362.1 predicted protein (5900 - 8962)
Length = 994
Score = 25.8 bits (55), Expect = 4.0
Identities = 9/28 (32%), Positives = 18/28 (64%)
Frame = -1
Query: 120 KKSPWTGERSETSHAHGSNGEDEASELR 37
+ S W+ +S++ HG++G +A+E R
Sbjct: 32 RPSVWSLSKSKSKDKHGASGRGDAAEFR 59
>NCU09333.1 NCU09333.1 hypothetical protein (31089 - 28765)
Length = 732
Score = 25.4 bits (54), Expect = 5.2
Identities = 9/25 (36%), Positives = 15/25 (60%)
Frame = +1
Query: 97 LSCPRRLLGSHVMCARDERKAEMLV 171
L CP+++ ++C RD K +LV
Sbjct: 209 LECPKKIQEKEILCLRDLEKTLILV 233
>NCU08154.1 NCU08154.1 predicted protein (40649 - 42112)
Length = 487
Score = 25.4 bits (54), Expect = 5.2
Identities = 14/33 (42%), Positives = 17/33 (51%), Gaps = 3/33 (9%)
Frame = -3
Query: 337 QEQSGNPTSI---DSENKDYYTPDPSVQPYEQE 248
+ +SG TS D+EN D P P V PY E
Sbjct: 147 ENESGYSTSTGTSDTENNDGGPPPPEVWPYGDE 179
>NCU10007.1 NCU10007.1 MALATE SYNTHASE, GLYOXYSOMAL (19263 - 17567)
Length = 542
Score = 25.4 bits (54), Expect = 5.2
Identities = 13/43 (30%), Positives = 26/43 (60%), Gaps = 7/43 (16%)
Frame = -1
Query: 315 PL*IRKTRITTP-------LIHQSNHTSRNSVLQQRQMNTAEL 208
P+ + +I TP L+H+S + +R ++L++RQ+ AE+
Sbjct: 16 PIEEHQRKILTPQALSFVALLHRSFNQTRKNLLERRQLRQAEI 58
>NCU08132.1 NCU08132.1 hypothetical protein (37900 - 45290)
Length = 2374
Score = 24.6 bits (52), Expect = 8.8
Identities = 11/28 (39%), Positives = 14/28 (49%)
Frame = -1
Query: 132 DMATKKSPWTGERSETSHAHGSNGEDEA 49
D+ T +PW G RSE+ A S A
Sbjct: 1709 DLPTPGAPWAGGRSESPGARDSTASSIA 1736
>NCU00046.1 NCU00046.1 hypothetical protein (34261 - 31619)
Length = 853
Score = 24.6 bits (52), Expect = 8.8
Identities = 13/23 (56%), Positives = 13/23 (56%)
Frame = +2
Query: 35 RRSSLASSSPLDPCACDVSDRSP 103
RRSS A SSP P A RSP
Sbjct: 291 RRSSKAQSSPTGPWAAPSPRRSP 313
>NCU00695.1 NCU00695.1 predicted protein (424527 - 426137)
Length = 536
Score = 24.6 bits (52), Expect = 8.8
Identities = 14/45 (31%), Positives = 19/45 (42%)
Frame = -1
Query: 156 LPLISRTHDMATKKSPWTGERSETSHAHGSNGEDEASELRRWAHH 22
LP + T +A +K TG E H H + D+ R HH
Sbjct: 301 LPSLFSTFSLALEK---TGLAEELGHGHDDDENDDDDNNDRKKHH 342
>NCU00691.1 NCU00691.1 hypothetical protein (411532 - 414017)
Length = 794
Score = 24.6 bits (52), Expect = 8.8
Identities = 9/25 (36%), Positives = 14/25 (56%)
Frame = +2
Query: 176 TASKAKRGSVVSSAVFICLCCRTEF 250
T +R + + + IC+CCR EF
Sbjct: 190 TGRLKRRKTYPALPIVICICCREEF 214
>NCU06684.1 NCU06684.1 hypothetical protein (17333 - 19505)
Length = 613
Score = 24.6 bits (52), Expect = 8.8
Identities = 14/48 (29%), Positives = 21/48 (43%), Gaps = 1/48 (2%)
Frame = -1
Query: 150 LISRTHDMATKKSPWTGERSETSHAHGSNGEDEASELRRW-AHHGPRP 10
+++R D + ERS+ H G + SEL W +G RP
Sbjct: 233 VLTRPMDARADVRAFLSERSKVLHMSGLPHDTTQSELESWFTQYGGRP 280
>NCU08896.1 NCU08896.1 hypothetical protein (57532 - 53806)
Length = 1022
Score = 24.6 bits (52), Expect = 8.8
Identities = 12/32 (37%), Positives = 15/32 (46%)
Frame = -1
Query: 99 ERSETSHAHGSNGEDEASELRRWAHHGPRPRP 4
+ S SH H E +S L + H P PRP
Sbjct: 344 DSSNISHWHSEVAEKGSSLLAKHLAHQPLPRP 375
>NCU09758.1 NCU09758.1 hypothetical protein (15938 - 12305)
Length = 1191
Score = 24.6 bits (52), Expect = 8.8
Identities = 20/53 (37%), Positives = 26/53 (48%), Gaps = 9/53 (16%)
Frame = +2
Query: 8 RGRGPWCAHR--------RSSLASSSPLDPCACDVSDRSPVQGDFLVA-MSCV 139
RG P A+R R SLA SSPL VS+R D LV+ ++C+
Sbjct: 346 RGTSPEIANRLSSATICHRVSLAGSSPLSQAHKLVSERLTKSQDILVSHLACL 398
Database: /phillip/Ncr/Ncr_P
Posted date: May 20, 2002 12:05 PM
Number of letters in database: 3,158,374
Number of sequences in database: 6531
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2060999
Number of Sequences: 6531
Number of extensions: 39606
Number of successful extensions: 182
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 175
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 182
length of query: 115
length of database: 3,158,374
effective HSP length: 42
effective length of query: 72
effective length of database: 2,884,072
effective search space: 207653184
effective search space used: 207653184
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)