BLASTX 2.1.3 [Apr-1-2001]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query=
/phillip/projects/Xu/Fgr-S/seq_dir/blast_NcrP/Fgr-S_3_B04_T7.seq
(327 letters)
Database: /phillip/Ncr/Ncr_P
6531 sequences; 3,158,374 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
NCU08128.1 NCU08128.1 hypothetical protein (23181 - 25240) 32 0.066
NCU01134.1 NCU01134.1 hypothetical protein ( cytochrome p450 rel... 30 0.15
NCU09419.1 NCU09419.1 hypothetical protein (16175 - 17910) 30 0.19
NCU09103.1 NCU09103.1 hypothetical protein (114647 - 112552) 29 0.33
NCU10003.1 NCU10003.1 hypothetical protein (16673 - 14476) 28 0.96
NCU06928.1 NCU06928.1 predicted protein (170505 - 173135) 27 1.2
NCU07378.1 NCU07378.1 hypothetical protein (42120 - 36200) 27 1.2
NCU09565.1 NCU09565.1 hypothetical protein (26616 - 24892) 27 1.2
NCU08112.1 NCU08112.1 hypothetical protein (26652 - 28518) 27 1.2
NCU06834.1 NCU06834.1 hypothetical protein (163075 - 159031) 27 1.6
NCU09636.1 NCU09636.1 hypothetical protein (5049 - 6870) 27 1.6
NCU08716.1 NCU08716.1 related to trichodiene oxygenase cytochrom... 26 2.8
NCU08062.1 NCU08062.1 hypothetical protein (46350 - 48069) 25 4.7
NCU10011.1 NCU10011.1 hypothetical protein (13303 - 11616) 25 6.2
NCU02762.1 NCU02762.1 hypothetical protein (13422 - 6705) 25 6.2
NCU00793.1 NCU00793.1 hypothetical protein (102276 - 99303) 25 8.1
NCU02624.1 NCU02624.1 hypothetical protein (2384 - 655) 25 8.1
NCU06526.1 NCU06526.1 hypothetical protein (11364 - 9643) 25 8.1
>NCU08128.1 NCU08128.1 hypothetical protein (23181 - 25240)
Length = 562
Score = 31.6 bits (70), Expect = 0.066
Identities = 15/35 (42%), Positives = 21/35 (59%)
Frame = -3
Query: 310 QVFGAGRRMCPGMNLGIANVELMLANLVCHFDWQL 206
Q F G R C G +L IA + L+L +V +FD +L
Sbjct: 495 QPFSVGPRNCIGRSLAIAEMRLILTKVVYNFDMKL 529
>NCU01134.1 NCU01134.1 hypothetical protein ( cytochrome p450
related protein [imported] - N
Length = 621
Score = 30.4 bits (67), Expect = 0.15
Identities = 19/69 (27%), Positives = 35/69 (50%), Gaps = 3/69 (4%)
Frame = -3
Query: 304 FGAGRRMCPGMNLGIANVELMLANLVCHFDWQLPLGVERKDIDMTEVF---GLTVHRKEK 134
FG GRR+C G L ++ + +A ++ ++ LP+G + D F G ++ K
Sbjct: 522 FGFGRRVCVGQYLAENSLLIAIAMMLATIEFDLPIGPDGNLKDFEPQFSHKGQSIVLPFK 581
Query: 133 LLLIPKSRM 107
+ + P+S M
Sbjct: 582 VSIKPRSPM 590
>NCU09419.1 NCU09419.1 hypothetical protein (16175 - 17910)
Length = 561
Score = 30.0 bits (66), Expect = 0.19
Identities = 14/39 (35%), Positives = 21/39 (52%)
Frame = -3
Query: 295 GRRMCPGMNLGIANVELMLANLVCHFDWQLPLGVERKDI 179
G R C G A + +LA V F+W+ LG++ KD+
Sbjct: 498 GPRSCIGQGFAKAELRCLLATFVLAFEWK--LGMDEKDV 534
>NCU09103.1 NCU09103.1 hypothetical protein (114647 - 112552)
Length = 523
Score = 29.3 bits (64), Expect = 0.33
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = -3
Query: 307 VFGAGRRMCPGMNLGIANVELMLANL 230
VFG G R+C GM L A + LML+ +
Sbjct: 456 VFGHGARVCLGMQLAYAEMYLMLSKM 481
>NCU10003.1 NCU10003.1 hypothetical protein (16673 - 14476)
Length = 639
Score = 27.7 bits (60), Expect = 0.96
Identities = 13/39 (33%), Positives = 18/39 (45%)
Frame = +3
Query: 138 SFRWTVSPNTSVISMSFLSTPSGSCQSKWHTRFANISST 254
S W+ SP+ V F+S PS + K +A I T
Sbjct: 28 SHTWSPSPDDEVTFQEFISLPSHELEKKKEKGYAKIKQT 66
>NCU06928.1 NCU06928.1 predicted protein (170505 - 173135)
Length = 853
Score = 27.3 bits (59), Expect = 1.2
Identities = 20/60 (33%), Positives = 28/60 (46%)
Frame = +1
Query: 148 GRLAQTPLSYQCPFSQPQAEVASQNGTQGLPT*APHSRSQGSYQGTSSVLLRTPARAAAR 327
G+LA P S P QP A+ A T A + G+ +G S+L +P+ AA R
Sbjct: 241 GKLAVHPTSQTQPTKQPSAQSAPSPAAAAPSTLA---KPAGTPRGIESLLSPSPSLAAPR 297
>NCU07378.1 NCU07378.1 hypothetical protein (42120 - 36200)
Length = 1943
Score = 27.3 bits (59), Expect = 1.2
Identities = 19/57 (33%), Positives = 26/57 (45%), Gaps = 1/57 (1%)
Frame = +1
Query: 124 STIVSPSDGRLAQTPLSYQCPFSQP-QAEVASQNGTQGLPT*APHSRSQGSYQGTSS 291
+T SPS+GRL PL + P +S T G T S + GS +G+ S
Sbjct: 1392 TTASSPSNGRLVMPPLPLSPQRTVPAHTLTSSPRNTSGPATGRARSLTVGSQEGSGS 1448
>NCU09565.1 NCU09565.1 hypothetical protein (26616 - 24892)
Length = 554
Score = 27.3 bits (59), Expect = 1.2
Identities = 13/44 (29%), Positives = 22/44 (49%)
Frame = -3
Query: 304 FGAGRRMCPGMNLGIANVELMLANLVCHFDWQLPLGVERKDIDM 173
FG G+ MCPG + ++A L F+ + G++R + M
Sbjct: 477 FGGGKHMCPGRHFAFTENLALMAALAIGFEIE---GLDRSKLKM 517
>NCU08112.1 NCU08112.1 hypothetical protein (26652 - 28518)
Length = 536
Score = 27.3 bits (59), Expect = 1.2
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = -3
Query: 307 VFGAGRRMCPGMNLGIANVELMLANLVCHFDWQL 206
VFG GR C G + + + + L+ +FD+QL
Sbjct: 466 VFGYGRWQCAGKMVAMLELNKIFCQLMRNFDFQL 499
>NCU06834.1 NCU06834.1 hypothetical protein (163075 - 159031)
Length = 1030
Score = 26.9 bits (58), Expect = 1.6
Identities = 14/27 (51%), Positives = 16/27 (58%), Gaps = 3/27 (11%)
Frame = +2
Query: 254 IRDPKV---HTRAHPPSCSEHLPGRPL 325
+R P V HTR +PP S HLP R L
Sbjct: 111 LRHPPVGDHHTRQYPPVDSHHLPFRSL 137
>NCU09636.1 NCU09636.1 hypothetical protein (5049 - 6870)
Length = 586
Score = 26.9 bits (58), Expect = 1.6
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = -3
Query: 316 PGQVFGAGRRMCPGMNLGIANVELMLANLVCHFD 215
P VFG G R C G L + ++++ LV +F+
Sbjct: 522 PQLVFGLGPRTCWGRRLAYMEMRIVISMLVWNFE 555
>NCU08716.1 NCU08716.1 related to trichodiene oxygenase cytochrome
P450 [MIPS] (67094 - 6509
Length = 529
Score = 26.2 bits (56), Expect = 2.8
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -3
Query: 304 FGAGRRMCPGMNLGIANVELMLANL 230
F G R C G NL +A + L+LA L
Sbjct: 464 FTKGSRSCLGQNLSMAEISLVLAVL 488
>NCU08062.1 NCU08062.1 hypothetical protein (46350 - 48069)
Length = 554
Score = 25.4 bits (54), Expect = 4.7
Identities = 9/29 (31%), Positives = 16/29 (55%)
Frame = -3
Query: 304 FGAGRRMCPGMNLGIANVELMLANLVCHF 218
F G R C G N + ++++L+ LV +
Sbjct: 486 FATGPRSCIGFNFALLEIKVLLSELVYRY 514
>NCU10011.1 NCU10011.1 hypothetical protein (13303 - 11616)
Length = 505
Score = 25.0 bits (53), Expect = 6.2
Identities = 21/77 (27%), Positives = 32/77 (41%), Gaps = 11/77 (14%)
Frame = -3
Query: 307 VFGAGRRMCPGMNLGIANVELMLANLVCHFDWQLPLGVERKDIDMT-----------EVF 161
VF G R C G + A ++ + C FD+ +P E + + V
Sbjct: 426 VFYGGPRTCLGRKMAEAIAAQVIPTMACLFDF-VPTSDEERTSKTSLTLPMEGGLPVTVK 484
Query: 160 GLTVHRKEKLLLIPKSR 110
LT +EKL+ IP+ R
Sbjct: 485 TLTGEEREKLVRIPEIR 501
>NCU02762.1 NCU02762.1 hypothetical protein (13422 - 6705)
Length = 2218
Score = 25.0 bits (53), Expect = 6.2
Identities = 16/53 (30%), Positives = 22/53 (41%), Gaps = 9/53 (16%)
Frame = +3
Query: 93 YFHDYMRDFGINNSFSFRW---------TVSPNTSVISMSFLSTPSGSCQSKW 224
Y +Y +G N FRW T+ VI+ FL TP+ +S W
Sbjct: 1227 YQKEYFEKYGENAFDWFRWVDTGFAIVFTLEALIKVIADGFLWTPNAYFRSTW 1279
>NCU00793.1 NCU00793.1 hypothetical protein (102276 - 99303)
Length = 909
Score = 24.6 bits (52), Expect = 8.1
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = -2
Query: 293 TEDVPWYEPWDRECGAYVGKPCVPF 219
TED ++PW E G V P +PF
Sbjct: 62 TEDRKHHDPWANEDGLKVQIPQMPF 86
>NCU02624.1 NCU02624.1 hypothetical protein (2384 - 655)
Length = 555
Score = 24.6 bits (52), Expect = 8.1
Identities = 11/32 (34%), Positives = 18/32 (55%)
Frame = -3
Query: 304 FGAGRRMCPGMNLGIANVELMLANLVCHFDWQ 209
FGAGR C G ++ +LA +V +F ++
Sbjct: 488 FGAGRHRCIGEQFANVQLQTILAIIVRNFKFR 519
>NCU06526.1 NCU06526.1 hypothetical protein (11364 - 9643)
Length = 523
Score = 24.6 bits (52), Expect = 8.1
Identities = 9/33 (27%), Positives = 18/33 (54%)
Frame = -3
Query: 304 FGAGRRMCPGMNLGIANVELMLANLVCHFDWQL 206
FG G +C G NL + + ++ L+ + ++L
Sbjct: 464 FGGGSHLCIGRNLALLEINKVVPRLLRDYRFEL 496
Database: /phillip/Ncr/Ncr_P
Posted date: May 20, 2002 12:05 PM
Number of letters in database: 3,158,374
Number of sequences in database: 6531
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2294297
Number of Sequences: 6531
Number of extensions: 48976
Number of successful extensions: 164
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 164
length of query: 109
length of database: 3,158,374
effective HSP length: 42
effective length of query: 66
effective length of database: 2,884,072
effective search space: 190348752
effective search space used: 190348752
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)