BLASTX 2.1.3 [Apr-1-2001]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query=
/phillip/projects/Xu/Fgr-S/seq_dir/blast_NcrP/Fgr-S_7_E02_T7.seq
(483 letters)
Database: /phillip/Ncr/Ncr_P
6531 sequences; 3,158,374 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
NCU02003.1 NCU02003.1 ELONGATION FACTOR 1-ALPHA (EF-1-ALPHA) (63... 149 4e-37
NCU09653.1 NCU09653.1 hypothetical protein (31473 - 30796) 29 0.59
NCU08616.1 NCU08616.1 DNA-DIRECTED RNA POLYMERASE I POLYPEPTIDE ... 25 8.5
NCU08148.1 NCU08148.1 hypothetical protein (20843 - 18510) 25 8.5
>NCU02003.1 NCU02003.1 ELONGATION FACTOR 1-ALPHA (EF-1-ALPHA) (63082
- 61334)
Length = 482
Score = 149 bits (376), Expect = 4e-37
Identities = 71/91 (78%), Positives = 80/91 (87%)
Frame = +3
Query: 21 IGNGYAPVLDCHTSHIAVKFAELVTKIDRRSGKELEALPKFLKNGDAGIVKMIPTKPMVV 200
+G GYAPVLDCHT+HIA KFAEL+ KIDRR+GK +EA PKF+K+GDA IVKMIP+KPM V
Sbjct: 374 VGAGYAPVLDCHTAHIACKFAELLEKIDRRTGKAVEASPKFIKSGDAAIVKMIPSKPMCV 433
Query: 201 ETFATYPPLGRFAVRDMRQTVAVGVIKGVEK 293
E F YPPLGRFAVRDMRQTVAVGVIK V+K
Sbjct: 434 EAFTDYPPLGRFAVRDMRQTVAVGVIKAVDK 464
>NCU09653.1 NCU09653.1 hypothetical protein (31473 - 30796)
Length = 225
Score = 29.3 bits (64), Expect = 0.59
Identities = 19/45 (42%), Positives = 26/45 (57%), Gaps = 1/45 (2%)
Frame = -3
Query: 322 LVTLAP-VGSFFSTPLMTPTATVCLMSRTAKRPRGG*VAKVSTTM 191
+ TL P VG+ F P+M + T+C+M+ TA G V V TTM
Sbjct: 133 IATLMPKVGNPFRVPVMVYSLTICVMALTALTLENGRVI-VGTTM 176
>NCU08616.1 NCU08616.1 DNA-DIRECTED RNA POLYMERASE I POLYPEPTIDE 2
(RNA POLYMERASE I SUBUNIT
Length = 1234
Score = 25.4 bits (54), Expect = 8.5
Identities = 11/24 (45%), Positives = 12/24 (49%), Gaps = 3/24 (12%)
Frame = +2
Query: 215 LPSSWSFCCPGHEAN---CGCWCH 277
LP SW F CP H + CG H
Sbjct: 527 LPESWGFLCPVHTPDGSPCGLLNH 550
>NCU08148.1 NCU08148.1 hypothetical protein (20843 - 18510)
Length = 628
Score = 25.4 bits (54), Expect = 8.5
Identities = 10/29 (34%), Positives = 11/29 (37%)
Frame = -1
Query: 189 AWWESSSLCQHHHS*GTWAGPPAPYQIAC 103
AWW +L H H W P Y C
Sbjct: 115 AWWIYGALIHHRHDHLGWLKPTLVYLAIC 143
Database: /phillip/Ncr/Ncr_P
Posted date: May 20, 2002 12:05 PM
Number of letters in database: 3,158,374
Number of sequences in database: 6531
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3105361
Number of Sequences: 6531
Number of extensions: 72739
Number of successful extensions: 221
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 213
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 221
length of query: 161
length of database: 3,158,374
effective HSP length: 42
effective length of query: 118
effective length of database: 2,884,072
effective search space: 340320496
effective search space used: 340320496
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)