The query sequence for this search has been filtered. Filtering
eliminates low complexity regions that commonly give spuriously high
scores that reflect compositional bias rather than significant
position-by-position alignment. Filtering can eliminate these potentially
confounding matches (e.g., hits against proline-rich regions or poly-A
tails) from the blast reports, leaving regions whose blast statistics
reflect the specificity of their pairwise alignment.
BLASTX 2.2.3 [Apr-24-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Fgr-S3_1_C05_T7.seq Fgr-S3_1_C05_T7 LENGTH:313bp ;
DIRECTION:5 ; CLONE:Fgr-S3_1_C05 ; CLONELIB: n/a
(313 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF
922,381 sequences; 289,601,141 total letters
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_510699.1| (NM_078298) ligand-gated ionic channel [Ca... 32 1.4
ref|NP_191447.1| (NM_115750) putative protein [Arabidopsis ... 30 4.0
gb|AAF06652.1|AF190043_1 (AF190043) NADH dehydrogenase subu... 30 5.3
emb|CAA05498.1| (AJ002490) ndhB [Arabidopsis thaliana] 25 5.7
sp|Q9T3G4|NU2C_ARATH NADH-plastoquinone oxidoreductase chai... 25 5.7
sp|Q9XQ96|NU2C_BRANA NADH-plastoquinone oxidoreductase chai... 25 5.7
gb|AAF06653.1|AF190044_1 (AF190044) NADH dehydrogenase subu... 29 9.0
ref|NP_505728.1| (NM_073327) UDP-glucuronosyltransferase [C... 29 9.0
>ref|NP_510699.1| (NM_078298) ligand-gated ionic channel [Caenorhabditis elegans]
pir||T30013 hypothetical protein T21F2.1 - Caenorhabditis elegans
gb|AAA83590.1| (U42836) Similar to ligand-gated ionic channel [Caenorhabditis
elegans]
Length = 398
Score = 32.0 bits (71), Expect = 1.4
Identities = 17/46 (36%), Positives = 25/46 (53%)
Frame = +3
Query: 42 WEQ*HPRFTFLRKYGNVAIEFPRGLPKYRECFLAWTSFHILLFDTK 179
W+Q +FTF R YG ++ +P Y F++W +F I DTK
Sbjct: 182 WDQLEVKFTFRRLYGYYVLQ--AYMPTYLSVFISWIAFWI---DTK 222
>ref|NP_191447.1| (NM_115750) putative protein [Arabidopsis thaliana]
pir||T49171 hypothetical protein T20N10.230 - Arabidopsis thaliana
emb|CAB88305.1| (AL353032) putative protein [Arabidopsis thaliana]
Length = 454
Score = 30.4 bits (67), Expect = 4.0
Identities = 22/66 (33%), Positives = 32/66 (48%)
Frame = +1
Query: 70 SYENMETSRSSFHGDCQNIVSVS*PGHPFTFSCLTRSLLCLVFTKYVDVSVLVILLELTV 249
SY N S +S VS+S P H F+F T +LLCL +T + ++ LE V
Sbjct: 198 SYPNATVSIASLKTLTIKSVSLSGPAHVFSFD--TPNLLCLNYTALFEDDYPLVNLEYLV 255
Query: 250 FYKLNF 267
++ F
Sbjct: 256 EAQIKF 261
>gb|AAF06652.1|AF190043_1 (AF190043) NADH dehydrogenase subunit 5 [Abax ovalis]
Length = 355
Score = 30.0 bits (66), Expect = 5.3
Identities = 16/58 (27%), Positives = 30/58 (51%), Gaps = 4/58 (6%)
Frame = +1
Query: 115 CQNIVSVS*PGHPFTFSCLTRSLLC-LVFTKYVDVSVLVILL---ELTVFYKLNFCYF 276
C NI +++ G PF ++ L+ ++ YV++S+ ++ LTV Y CY+
Sbjct: 122 CMNISNLALCGMPFLAGFYSKDLILEVICMDYVNISIFLLFFISTGLTVCYSFRLCYY 179
>emb|CAA05498.1| (AJ002490) ndhB [Arabidopsis thaliana]
Length = 512
Score = 24.6 bits (52), Expect(2) = 5.7
Identities = 9/14 (64%), Positives = 11/14 (78%)
Frame = +3
Query: 150 SFHILLFDTKFIMP 191
+FH+LLFD FI P
Sbjct: 21 AFHLLLFDGSFIFP 34
Score = 23.9 bits (50), Expect(2) = 5.7
Identities = 9/21 (42%), Positives = 14/21 (65%)
Frame = +2
Query: 224 W*YCLN*LSFIS*TSAILFRW 286
W Y ++ SF+ +A+LFRW
Sbjct: 59 WLYFISSTSFVMSITALLFRW 79
>sp|Q9T3G4|NU2C_ARATH NADH-plastoquinone oxidoreductase chain 2, chloroplast
Length = 512
Score = 24.6 bits (52), Expect(2) = 5.7
Identities = 9/14 (64%), Positives = 11/14 (78%)
Frame = +3
Query: 150 SFHILLFDTKFIMP 191
+FH+LLFD FI P
Sbjct: 21 AFHLLLFDGSFIFP 34
Score = 23.9 bits (50), Expect(2) = 5.7
Identities = 9/21 (42%), Positives = 14/21 (65%)
Frame = +2
Query: 224 W*YCLN*LSFIS*TSAILFRW 286
W Y ++ SF+ +A+LFRW
Sbjct: 59 WLYFISSTSFVMSITALLFRW 79
>sp|Q9XQ96|NU2C_BRANA NADH-plastoquinone oxidoreductase chain 2, chloroplast
gb|AAD28444.1|AF126026_1 (AF126026) NADH dehydrogenase subunit 2 [Brassica napus]
Length = 510
Score = 24.6 bits (52), Expect(2) = 5.7
Identities = 9/14 (64%), Positives = 11/14 (78%)
Frame = +3
Query: 150 SFHILLFDTKFIMP 191
+FH+LLFD FI P
Sbjct: 21 AFHLLLFDGSFIFP 34
Score = 23.9 bits (50), Expect(2) = 5.7
Identities = 9/21 (42%), Positives = 14/21 (65%)
Frame = +2
Query: 224 W*YCLN*LSFIS*TSAILFRW 286
W Y ++ SF+ +A+LFRW
Sbjct: 59 WLYFISSTSFVMSITALLFRW 79
>gb|AAF06653.1|AF190044_1 (AF190044) NADH dehydrogenase subunit 5 [Abax parallelepipedus
inferior]
Length = 355
Score = 29.3 bits (64), Expect = 9.0
Identities = 17/58 (29%), Positives = 29/58 (49%), Gaps = 4/58 (6%)
Frame = +1
Query: 115 CQNIVSVS*PGHPFTFSCLTRSLLC-LVFTKYVDVSVLVILLE---LTVFYKLNFCYF 276
C NI S++ G PF ++ L+ +V YV++ + ++ LTV Y CY+
Sbjct: 122 CMNISSLALCGMPFLAGFYSKDLILEVVCMDYVNIFIFMLFFVSTGLTVCYSFRLCYY 179
>ref|NP_505728.1| (NM_073327) UDP-glucuronosyltransferase [Caenorhabditis elegans]
pir||T19638 hypothetical protein F29F11.2 - Caenorhabditis elegans
emb|CAA98112.1| (Z73905) contains similarity to Pfam domain: PF00201
(UDP-glucoronosyl and UDP-glucosyl transferases),
Score=176.0, E-value=2e-49, N=2 [Caenorhabditis elegans]
emb|CAA98274.1| (Z73974) contains similarity to Pfam domain: PF00201
(UDP-glucoronosyl and UDP-glucosyl transferases),
Score=176.0, E-value=2e-49, N=2 [Caenorhabditis elegans]
Length = 1003
Score = 29.3 bits (64), Expect = 9.0
Identities = 15/41 (36%), Positives = 20/41 (48%)
Frame = +3
Query: 57 PRFTFLRKYGNVAIEFPRGLPKYRECFLAWTSFHILLFDTK 179
P TF+ KY N +EF LP + F W LL D++
Sbjct: 327 PSVTFIWKYENNNVEFAENLPNFH--FFKWVPQTALLADSR 365
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF
Posted date: May 11, 2002 4:54 AM
Number of letters in database: 289,601,141
Number of sequences in database: 922,381
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.267 0.0410 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 171,483,799
Number of Sequences: 922381
Number of extensions: 3254973
Number of successful extensions: 6963
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 6872
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6962
length of database: 289,601,141
effective HSP length: 79
effective length of database: 216,733,042
effective search space used: 5201593008
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)