The query sequence for this search has been filtered. Filtering
eliminates low complexity regions that commonly give spuriously high
scores that reflect compositional bias rather than significant
position-by-position alignment. Filtering can eliminate these potentially
confounding matches (e.g., hits against proline-rich regions or poly-A
tails) from the blast reports, leaving regions whose blast statistics
reflect the specificity of their pairwise alignment.
BLASTX 2.2.3 [Apr-24-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Fgr-S3_1_C20_T7.seq Fgr-S3_1_C20_T7 LENGTH:551bp ;
DIRECTION:5 ; CLONE:Fgr-S3_1_C20 ; CLONELIB: n/a
(551 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF
922,381 sequences; 289,601,141 total letters
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_273254.1| (NC_003112) ribonuclease E [Neisseria meni... 34 0.70
gb|AAF49825.1| (AE003538) CG10191 gene product [Drosophila ... 33 2.0
gb|AAC32042.1| (AF033663) PRP4 protein kinase homolog [Mus ... 32 4.5
gb|AAM19102.1|AF283466_1 (AF283466) PRP4 kinase [Mus musculus] 32 4.5
emb|CAB51045.2| (AJ009579) alkane 1-monooxygenase [Pseudomo... 31 5.9
ref|NP_219374.1| (NC_000919) T. pallidum predicted coding r... 31 7.7
>ref|NP_273254.1| (NC_003112) ribonuclease E [Neisseria meningitidis MC58]
pir||F81225 ribonuclease E NMB0196 [imported] - Neisseria meningitidis (group
B strain MD58)
gb|AAF40653.1| (AE002377) ribonuclease E [Neisseria meningitidis MC58]
Length = 919
Score = 34.3 bits (77), Expect = 0.70
Identities = 22/83 (26%), Positives = 37/83 (44%)
Frame = +1
Query: 70 TWRGTVRAWLTYMIVSLASI*HRTRANMNSSKPKVAQTHKDSHT*PNNKRASQQRMNPSR 249
+W + +AWL + + + P + K S + RA+ +R NP R
Sbjct: 546 SWWDSFKAWLKRIF----------GGSETQAAPAAETSEKRSTANRSGSRANNRRQNPRR 595
Query: 250 SKLQGLKGTMRDISLKGRTQEQR 318
SK +G K +R+++ K QE R
Sbjct: 596 SKREGSKVEVREVAGKTAGQEAR 618
>gb|AAF49825.1| (AE003538) CG10191 gene product [Drosophila melanogaster]
gb|AAL39494.1| (AY069349) LD05671p [Drosophila melanogaster]
Length = 391
Score = 32.7 bits (73), Expect = 2.0
Identities = 10/26 (38%), Positives = 17/26 (64%)
Frame = -1
Query: 128 MDASDTIIYVSQARTVPRQVRWHPWG 51
+D+ + + ++ R PRQ+ WHPWG
Sbjct: 173 VDSGECVRTFTEERAAPRQLAWHPWG 198
>gb|AAC32042.1| (AF033663) PRP4 protein kinase homolog [Mus musculus]
Length = 823
Score = 31.6 bits (70), Expect = 4.5
Identities = 18/56 (32%), Positives = 28/56 (49%)
Frame = +1
Query: 157 SSKPKVAQTHKDSHT*PNNKRASQQRMNPSRSKLQGLKGTMRDISLKGRTQEQRRI 324
S PK+ + S + N R S+Q +PSR+ G + R + K R E+RR+
Sbjct: 170 SLSPKLRDKSRRSRSPLLNDRRSKQSKSPSRTLSPGRRAKSRSLERKRREPERRRL 225
>gb|AAM19102.1|AF283466_1 (AF283466) PRP4 kinase [Mus musculus]
Length = 1007
Score = 31.6 bits (70), Expect = 4.5
Identities = 18/56 (32%), Positives = 28/56 (49%)
Frame = +1
Query: 157 SSKPKVAQTHKDSHT*PNNKRASQQRMNPSRSKLQGLKGTMRDISLKGRTQEQRRI 324
S PK+ + S + N R S+Q +PSR+ G + R + K R E+RR+
Sbjct: 354 SLSPKLRDKSRRSRSPLLNDRRSKQSKSPSRTLSPGRRAKSRSLERKRREPERRRL 409
>emb|CAB51045.2| (AJ009579) alkane 1-monooxygenase [Pseudomonas fluorescens]
Length = 430
Score = 31.2 bits (69), Expect = 5.9
Identities = 15/43 (34%), Positives = 29/43 (66%)
Frame = -3
Query: 444 GFDTLVVYSW*PSLWGNVS*CFHRAMVVPYYSGTLKLDGMYPS 316
G+ T++V+++ P+LW + HR V+ +YSG ++L M+P+
Sbjct: 372 GYATMIVWAYVPALWRRLM--DHR--VLAHYSGDVRLANMHPA 410
>ref|NP_219374.1| (NC_000919) T. pallidum predicted coding region TP0938 [Treponema
pallidum]
sp|O83908|Y938_TREPA Hypothetical lipoprotein TP0938 precursor
pir||F71262 hypothetical protein TP0938 - syphilis spirochete
gb|AAC65898.1| (AE001262) T. pallidum predicted coding region TP0938 [Treponema
pallidum]
Length = 276
Score = 30.8 bits (68), Expect = 7.7
Identities = 16/41 (39%), Positives = 24/41 (58%)
Frame = -1
Query: 314 CSCVRPFREMSRIVPLSPCSLLRLGFIRC*LARLLLGYVWL 192
CSCVR E S +VP+SP S ++ F+ + G+V+L
Sbjct: 29 CSCVRESTERSGVVPISPSSQQKVLFV---FGKKYSGHVYL 66
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF
Posted date: May 11, 2002 4:54 AM
Number of letters in database: 289,601,141
Number of sequences in database: 922,381
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.267 0.0410 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 331,578,751
Number of Sequences: 922381
Number of extensions: 7880114
Number of successful extensions: 18952
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 18354
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 18941
length of database: 289,601,141
effective HSP length: 113
effective length of database: 185,372,088
effective search space used: 12976046160
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)