The query sequence for this search has been filtered. Filtering
eliminates low complexity regions that commonly give spuriously high
scores that reflect compositional bias rather than significant
position-by-position alignment. Filtering can eliminate these potentially
confounding matches (e.g., hits against proline-rich regions or poly-A
tails) from the blast reports, leaving regions whose blast statistics
reflect the specificity of their pairwise alignment.
BLASTX 2.2.3 [Apr-24-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Fgr-S3_1_D05_T7.seq Fgr-S3_1_D05_T7 LENGTH:641bp ;
DIRECTION:5 ; CLONE:Fgr-S3_1_D05 ; CLONELIB: n/a
(641 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF
922,381 sequences; 289,601,141 total letters
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_001812.1| (NM_001821) choroideremia-like Rab escort ... 33 2.8
pir||S38787 CHML protein - human >gi|12043385|emb|CAC19784.... 33 2.8
ref|XP_102551.1| (XM_102551) hypothetical protein XP_102551... 31 8.2
gb|AAG22347.1| (AE003490) CG1627 gene product [Drosophila m... 31 8.2
>ref|NP_001812.1| (NM_001821) choroideremia-like Rab escort protein 2; REP-2, Rab
escort protein 2; choroideremia-like [Homo sapiens]
sp|P26374|RAE2_HUMAN Rab proteins geranylgeranyltransferase component A 2 (Rab escort
protein 2) (REP-2) (Choroideraemia-like protein)
emb|CAA45979.1| (X64728) hCHML [Homo sapiens]
Length = 656
Score = 32.7 bits (73), Expect = 2.8
Identities = 15/35 (42%), Positives = 20/35 (56%), Gaps = 3/35 (8%)
Frame = +1
Query: 337 CAPTGILCCQHYKLSC---SPEDGRCRALIHHSGQ 432
CA G + C +K+ C E GRC+A+I H GQ
Sbjct: 393 CAVFGGIYCLRHKVQCFVVDKESGRCKAIIDHFGQ 427
>pir||S38787 CHML protein - human
emb|CAC19784.1| (AL133390) dJ317G22.3 (choroideremia-like (Rab escort protein 2))
[Homo sapiens]
Length = 656
Score = 32.7 bits (73), Expect = 2.8
Identities = 15/35 (42%), Positives = 20/35 (56%), Gaps = 3/35 (8%)
Frame = +1
Query: 337 CAPTGILCCQHYKLSC---SPEDGRCRALIHHSGQ 432
CA G + C +K+ C E GRC+A+I H GQ
Sbjct: 393 CAVFGGIYCLRHKVQCFVVDKESGRCKAIIDHFGQ 427
>ref|XP_102551.1| (XM_102551) hypothetical protein XP_102551 [Homo sapiens]
Length = 212
Score = 31.2 bits (69), Expect = 8.2
Identities = 23/74 (31%), Positives = 31/74 (41%), Gaps = 1/74 (1%)
Frame = +3
Query: 270 KPGRSRQAWTLSFLQSAMR*PCM-CSDWHLVLPALQAILLA*RWXVSRIDTSLWPRAAFQ 446
+P W S Q PCM C DWH +LP A +A W T + P +
Sbjct: 124 QPAGDASTWRFSEPQM----PCMSCVDWHDILPVCVASKVA-EW-----GTLVGPGGVSE 173
Query: 447 NDIESLMTRLNKDP 488
DI+ ++ RL P
Sbjct: 174 EDIKGILARLGLLP 187
>gb|AAG22347.1| (AE003490) CG1627 gene product [Drosophila melanogaster]
Length = 751
Score = 31.2 bits (69), Expect = 8.2
Identities = 15/45 (33%), Positives = 26/45 (57%)
Frame = +3
Query: 36 PRGDLSEEG*GDSEQATGKHVSSEKRPGSLSESTRQEALPYARSY 170
P + + G + TG V++++ P SLS STRQ+A+ ++ Y
Sbjct: 627 PSNNTLQSAGGGAGLTTGLEVTAQRVPPSLSGSTRQDAVAISKGY 671
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF
Posted date: May 11, 2002 4:54 AM
Number of letters in database: 289,601,141
Number of sequences in database: 922,381
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.267 0.0410 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 335,540,204
Number of Sequences: 922381
Number of extensions: 6660511
Number of successful extensions: 16455
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 15864
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 16455
length of database: 289,601,141
effective HSP length: 115
effective length of database: 183,527,326
effective search space used: 17985677948
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)