The query sequence for this search has been filtered. Filtering
eliminates low complexity regions that commonly give spuriously high
scores that reflect compositional bias rather than significant
position-by-position alignment. Filtering can eliminate these potentially
confounding matches (e.g., hits against proline-rich regions or poly-A
tails) from the blast reports, leaving regions whose blast statistics
reflect the specificity of their pairwise alignment.
BLASTX 2.2.3 [Apr-24-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Fgr-S3_1_D07_T7.seq Fgr-S3_1_D07_T7 LENGTH:250bp ;
DIRECTION:5 ; CLONE:Fgr-S3_1_D07 ; CLONELIB: n/a
(250 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF
922,381 sequences; 289,601,141 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AAH12494.1|AAH12494 (BC012494) Unknown (protein for MGC:... 30 7.5
pir||T37125 hypothetical protein SCJ4.42c - Streptomyces co... 30 7.5
dbj|BAB12135.1| (AB047611) hypothetical protein [Macaca fas... 29 9.8
ref|NP_495639.1| (NM_063238) Glutamate receptor [Caenorhabd... 29 9.8
ref|NP_561294.1| (NC_003366) probable myosin-crossreactive ... 29 9.8
ref|XP_010289.5| (XM_010289) DNA segment, numerous copies, ... 29 9.8
gb|AAK19738.2|AF346629_1 (AF346629) channel-kinase 1 [Homo ... 29 9.8
ref|NP_036212.1| (NM_012080) DNA segment, numerous copies, ... 29 9.8
gb|AAK44211.1| (AY032950) LTRPC7 [Homo sapiens] 29 9.8
>gb|AAH12494.1|AAH12494 (BC012494) Unknown (protein for MGC:21172) [Homo sapiens]
Length = 214
Score = 29.6 bits (65), Expect = 7.5
Identities = 19/67 (28%), Positives = 30/67 (44%), Gaps = 2/67 (2%)
Frame = +3
Query: 51 LPEEQRWTIQVQVPGTTFMPVTEPIEFSFDAPTWSLRKIMA--SHIVMGRVGEVYHNDLD 224
+P ++ I ++ G F T SFD T ++ + SHIV+G EV H D
Sbjct: 78 MPGAEKLIIHLRKHGIPFALATSSRSASFDMKTSRHKEFFSLFSHIVLGDDPEVQHGKPD 137
Query: 225 DAVYQIC 245
++ C
Sbjct: 138 PDIFLAC 144
>pir||T37125 hypothetical protein SCJ4.42c - Streptomyces coelicolor
emb|CAB52976.1| (AL109950) conserved hypothetical protein [Streptomyces coelicolor
A3(2)]
Length = 434
Score = 29.6 bits (65), Expect = 7.5
Identities = 16/55 (29%), Positives = 25/55 (45%)
Frame = +3
Query: 54 PEEQRWTIQVQVPGTTFMPVTEPIEFSFDAPTWSLRKIMASHIVMGRVGEVYHND 218
P+E W I + V G T TE W+L ++A+ G++ E +H D
Sbjct: 351 PDEHIWPIAIAVQGLTSNDTTE--------RRWALTTLLATDAGTGQMHESFHKD 397
>dbj|BAB12135.1| (AB047611) hypothetical protein [Macaca fascicularis]
Length = 640
Score = 29.3 bits (64), Expect = 9.8
Identities = 16/47 (34%), Positives = 24/47 (51%), Gaps = 5/47 (10%)
Frame = +3
Query: 123 IEFSFDAPTWSLRKIMASHIVMGRVGEVYHNDL-----DDAVYQICG 248
I + +AP+W+L K + H GEVY ++ D + QICG
Sbjct: 240 ILYPHEAPSWTLAKDIVFHPYWMIFGEVYAYEIDVCANDSVIPQICG 286
>ref|NP_495639.1| (NM_063238) Glutamate receptor [Caenorhabditis elegans]
gb|AAA81054.2| (U39849) C. elegans GLR-4 protein (corresponding sequence C06A8.9)
[Caenorhabditis elegans]
Length = 950
Score = 29.3 bits (64), Expect = 9.8
Identities = 14/40 (35%), Positives = 20/40 (50%)
Frame = -3
Query: 200 SNTSHDDVRCHDLS*TPSWSIKRKLYGLGDWHERRSWNLN 81
SN DD+ H + S +K LG+W E+R WN +
Sbjct: 353 SNGERDDIMYHGVGRINSQFVK-----LGNWSEKRGWNFD 387
>ref|NP_561294.1| (NC_003366) probable myosin-crossreactive antigen [Clostridium
perfringens]
dbj|BAB80084.1| (AP003186) probable myosin-crossreactive antigen [Clostridium
perfringens]
Length = 597
Score = 29.3 bits (64), Expect = 9.8
Identities = 13/60 (21%), Positives = 29/60 (47%), Gaps = 3/60 (5%)
Frame = -3
Query: 242 NLVDSIIEIIVV---DFSNTSHDDVRCHDLS*TPSWSIKRKLYGLGDWHERRSWNLNLNR 72
+L + E +++ + + S D++ +L T W++ R ++ +WH L +NR
Sbjct: 153 DLAKELAEFVLIPDEELQDKSIDEIFSEELLNTDFWTLWRTMFAFENWHSALEMKLYMNR 212
>ref|XP_010289.5| (XM_010289) DNA segment, numerous copies, expressed probes (GS1
gene) [Homo sapiens]
Length = 214
Score = 29.3 bits (64), Expect = 9.8
Identities = 19/67 (28%), Positives = 30/67 (44%), Gaps = 2/67 (2%)
Frame = +3
Query: 51 LPEEQRWTIQVQVPGTTFMPVTEPIEFSFDAPTWSLRKIMA--SHIVMGRVGEVYHNDLD 224
+P ++ I ++ G F T SFD T ++ + SHIV+G EV H D
Sbjct: 78 MPGAEKLIIHLRKHGIPFALATSSGSASFDMKTSRHKEFFSLFSHIVLGDDPEVQHGKPD 137
Query: 225 DAVYQIC 245
++ C
Sbjct: 138 PDIFLAC 144
>gb|AAK19738.2|AF346629_1 (AF346629) channel-kinase 1 [Homo sapiens]
Length = 1864
Score = 29.3 bits (64), Expect = 9.8
Identities = 16/47 (34%), Positives = 24/47 (51%), Gaps = 5/47 (10%)
Frame = +3
Query: 123 IEFSFDAPTWSLRKIMASHIVMGRVGEVYHNDL-----DDAVYQICG 248
I + +AP+W+L K + H GEVY ++ D + QICG
Sbjct: 1021 ILYPHEAPSWTLAKDIVFHPYWMIFGEVYAYEIDVCANDSVIPQICG 1067
>ref|NP_036212.1| (NM_012080) DNA segment, numerous copies, expressed probes (GS1
gene) [Homo sapiens]
sp|Q08623|GS1_HUMAN GS1 protein
gb|AAA58622.1| (M86934) Gene from Xp22.3 which escapes X-inactivation. Function
unknown [Homo sapiens]
Length = 214
Score = 29.3 bits (64), Expect = 9.8
Identities = 19/67 (28%), Positives = 30/67 (44%), Gaps = 2/67 (2%)
Frame = +3
Query: 51 LPEEQRWTIQVQVPGTTFMPVTEPIEFSFDAPTWSLRKIMA--SHIVMGRVGEVYHNDLD 224
+P ++ I ++ G F T SFD T ++ + SHIV+G EV H D
Sbjct: 78 MPGAEKLIIHLRKHGIPFALATSSGSASFDMKTSRHKEFFSLFSHIVLGDDPEVQHGKPD 137
Query: 225 DAVYQIC 245
++ C
Sbjct: 138 PDIFLAC 144
>gb|AAK44211.1| (AY032950) LTRPC7 [Homo sapiens]
Length = 1865
Score = 29.3 bits (64), Expect = 9.8
Identities = 16/47 (34%), Positives = 24/47 (51%), Gaps = 5/47 (10%)
Frame = +3
Query: 123 IEFSFDAPTWSLRKIMASHIVMGRVGEVYHNDL-----DDAVYQICG 248
I + +AP+W+L K + H GEVY ++ D + QICG
Sbjct: 1021 ILYPHEAPSWTLAKDIVFHPYWMIFGEVYAYEIDVCANDSVIPQICG 1067
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF
Posted date: May 11, 2002 4:54 AM
Number of letters in database: 289,601,141
Number of sequences in database: 922,381
Lambda K H
0.318 0.135 0.00
Gapped
Lambda K H
0.267 0.0410 4.94e-324
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 145,423,800
Number of Sequences: 922381
Number of extensions: 2872090
Number of successful extensions: 8941
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 8811
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8936
length of database: 289,601,141
effective HSP length: 58
effective length of database: 236,103,043
effective search space used: 5666473032
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)